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Protein

Phospholipase A2, membrane associated

Gene

Pla2g2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides (PubMed:3356705). Thought to participate in the regulation of phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Acts as a ligand for integrins. Binds to and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1. Independent of its catalytic activity, activates integrins by binding to a site (site 2) which is distinct from the classical ligand-binding site (site 1) and inducing integrin conformational changes and enhanced ligand binding to site 1. Induces cell proliferation in an integrin-dependent manner (By similarity).By similarity1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation1 Publication

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi48Calcium; via carbonyl oxygenBy similarity1
Metal bindingi50Calcium; via carbonyl oxygenBy similarity1
Metal bindingi52Calcium; via carbonyl oxygenBy similarity1
Active sitei68By similarity1
Metal bindingi69CalciumBy similarity1
Active sitei113By similarity1
Sitei122Important for integrin bindingBy similarity1

GO - Molecular functioni

  • calcium-dependent phospholipase A2 activity Source: RGD
  • calcium ion binding Source: InterPro
  • phospholipase A2 activity Source: RGD
  • phospholipid binding Source: Ensembl

GO - Biological processi

  • arachidonic acid secretion Source: InterPro
  • lipid catabolic process Source: RGD
  • phosphatidic acid metabolic process Source: Ensembl
  • phospholipid metabolic process Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.
R-RNO-1482925. Acyl chain remodelling of PG.
R-RNO-1483166. Synthesis of PA.
R-RNO-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2, membrane associated (EC:3.1.1.41 Publication)
Alternative name(s):
GIIC sPLA2
Group IIA phospholipase A2
Phosphatidylcholine 2-acylhydrolase 2A
Gene namesi
Name:Pla2g2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi620857. Pla2g2a.

Subcellular locationi

GO - Cellular componenti

  • endoplasmic reticulum Source: Ensembl
  • extracellular exosome Source: Ensembl
  • extracellular space Source: Ensembl
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: UniProtKB-SubCell
  • secretory granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3686.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 214 PublicationsAdd BLAST21
ChainiPRO_000002275222 – 146Phospholipase A2, membrane associatedAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 139By similarity
Disulfide bondi49 ↔ 65By similarity
Disulfide bondi64 ↔ 119By similarity
Disulfide bondi70 ↔ 146By similarity
Disulfide bondi71 ↔ 112By similarity
Disulfide bondi80 ↔ 105By similarity
Disulfide bondi98 ↔ 110By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP14423.
PRIDEiP14423.

Expressioni

Gene expression databases

BgeeiENSRNOG00000016945.
GenevisibleiP14423. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022827.

Chemistry databases

BindingDBiP14423.

Structurei

3D structure databases

ProteinModelPortaliP14423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP14423.
KOiK01047.
OMAiKQDSCRS.
OrthoDBiEOG091G0UZ3.
PhylomeDBiP14423.
TreeFamiTF319283.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVLLLLAVV IMAFGSIQVQ GSLLEFGQMI LFKTGKRADV SYGFYGCHCG
60 70 80 90 100
VGGRGSPKDA TDWCCVTHDC CYNRLEKRGC GTKFLTYKFS YRGGQISCST
110 120 130 140
NQDSCRKQLC QCDKAAAECF ARNKKSYSLK YQFYPNKFCK GKTPSC
Length:146
Mass (Da):16,294
Last modified:February 1, 1991 - v2
Checksum:i60DDC9E79BF109F7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22S → D AA sequence (PubMed:2722857).Curated1
Sequence conflicti63W → E AA sequence (PubMed:3235451).Curated1
Sequence conflicti69D → E AA sequence (PubMed:3235451).Curated1
Sequence conflicti78R → S AA sequence (PubMed:3235451).Curated1
Sequence conflicti85L → V in AAA41920 (PubMed:2764915).Curated1
Sequence conflicti121A → S AA sequence (PubMed:3235451).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti135P → L Polymorphism. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00523 mRNA. Translation: BAA00410.1.
M37127 Genomic DNA. Translation: AAA41223.1.
M25148 mRNA. Translation: AAA41920.1.
X51529 Genomic DNA. Translation: CAA35909.1.
PIRiA33394.
A35493.
JU0283.
JX0052.
RefSeqiNP_113786.3. NM_031598.3.
XP_006239208.1. XM_006239146.2.
UniGeneiRn.11346.

Genome annotation databases

EnsembliENSRNOT00000022827; ENSRNOP00000022827; ENSRNOG00000016945.
GeneIDi29692.
KEGGirno:29692.
UCSCiRGD:620857. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00523 mRNA. Translation: BAA00410.1.
M37127 Genomic DNA. Translation: AAA41223.1.
M25148 mRNA. Translation: AAA41920.1.
X51529 Genomic DNA. Translation: CAA35909.1.
PIRiA33394.
A35493.
JU0283.
JX0052.
RefSeqiNP_113786.3. NM_031598.3.
XP_006239208.1. XM_006239146.2.
UniGeneiRn.11346.

3D structure databases

ProteinModelPortaliP14423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022827.

Chemistry databases

BindingDBiP14423.
ChEMBLiCHEMBL3686.

Proteomic databases

PaxDbiP14423.
PRIDEiP14423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022827; ENSRNOP00000022827; ENSRNOG00000016945.
GeneIDi29692.
KEGGirno:29692.
UCSCiRGD:620857. rat.

Organism-specific databases

CTDi5320.
RGDi620857. Pla2g2a.

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP14423.
KOiK01047.
OMAiKQDSCRS.
OrthoDBiEOG091G0UZ3.
PhylomeDBiP14423.
TreeFamiTF319283.

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.
R-RNO-1482925. Acyl chain remodelling of PG.
R-RNO-1483166. Synthesis of PA.
R-RNO-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiP14423.

Gene expression databases

BgeeiENSRNOG00000016945.
GenevisibleiP14423. RN.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2GA_RAT
AccessioniPrimary (citable) accession number: P14423
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1991
Last modified: November 30, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Group II phospholipase A2 is found in many cells and also extracellularly. The membrane-bound and secreted forms are identical and are encoded by a single gene.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.