P14419 (PA21B_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase A2
EC=3.1.1.4

Alternative name(s):
Group IB phospholipase A2
Phosphatidylcholine 2-acylhydrolase 1B

Gene names

Name:

PLA2G1B

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	123 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Catalytic activity	Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the phospholipase A2 family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW phospholipid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 123	123	Phospholipase A2		PRO_0000161588
Sites
	Active site	48	1	By similarity
	Active site	99	1	By similarity
	Metal binding	28	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	30	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	32	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	49	1	Calcium By similarity
Amino acid modifications
	Disulfide bond	11 ↔ 77		By similarity
	Disulfide bond	27 ↔ 123		By similarity
	Disulfide bond	29 ↔ 45		By similarity
	Disulfide bond	44 ↔ 105		By similarity
	Disulfide bond	51 ↔ 98		By similarity
	Disulfide bond	61 ↔ 91		By similarity
	Disulfide bond	84 ↔ 96		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14419 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: F69878C15DF6C4F9	FASTA	123	13,817
	10 20 30 40 50 60 ALWQFNGMIK CKIPSSEPLL DFNNYGCYCG LGGSGTPVDD LDRCCQTHDN CYKQAKKLDS 70 80 90 100 110 120 CKVLVDNPYT NSYSYSCSNK QITCSSENNA CEAFICNCDR NAAICFSEVP YNNEHKNLDK KYC

References

[1]	"The primary structure and some kinetic properties of phospholipase A2 from sheep pancreas." Verheij H.M., Raykova D., Dijkman R., Lenting H.B.M., de Haas G.H. Recl. Trav. Chim. Pays-Bas 107:203-207(1988) Cited for: PROTEIN SEQUENCE. Tissue: Pancreas.
+	Additional computationally mapped references.

Cross-references

3D structure databases
ProteinModelPortal	P14419.
SMR	P14419. Positions 1-123.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008137.
Family and domain databases
InterPro	IPR016090. PLipase_A2. IPR013090. PLipase_A2_AS. IPR001211. PLipase_A2_euk. [Graphical view]
Gene3D	G3DSA:1.20.90.10. Phospholipase_A2. 1 hit.
PANTHER	PTHR11716. Phospholipase_A2. 1 hit.
Pfam	PF00068. Phospholip_A2_1. 1 hit. [Graphical view]
PRINTS	PR00389. PHPHLIPASEA2.
SMART	SM00085. PA2c. 1 hit. [Graphical view]
SUPFAM	SSF48619. PhospholipaseA2. 1 hit.
PROSITE	PS00119. PA2_ASP. 1 hit. PS00118. PA2_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA21B_SHEEP

Accession

Primary (citable) accession number: P14419

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	October 19, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program