P14415 (AT1B2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-2

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-2

Gene names

Name:

ATP1B2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-2 subunit is not known.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro blood coagulation Traceable author statement. Source: Reactome leukocyte migration Traceable author statement. Source: Reactome
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB sodium:potassium-exchanging ATPase activity Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Sodium/potassium-transporting ATPase subunit beta-2		PRO_0000219104
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 67	28	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	68 – 290	223	Extracellular Potential
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Potential
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	159	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Ref.8
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Disulfide bond	129 ↔ 150		By similarity
	Disulfide bond	160 ↔ 177		By similarity
	Disulfide bond	200 ↔ 261		By similarity
Natural variations
	Natural variant	124	1	Q → L. Corresponds to variant rs34745087 [ dbSNP | Ensembl ].		VAR_061031
	Natural variant	199	1	T → A. Corresponds to variant rs2227866 [ dbSNP | Ensembl ].		VAR_030339
Experimental info
	Sequence conflict	51	1	L → P in AAA51805. Ref.1
	Sequence conflict	51	1	L → P no nucleotide entry Ref.6
	Sequence conflict	121	1	I → M in AAA51805. Ref.1
	Sequence conflict	121	1	I → M no nucleotide entry Ref.6
	Sequence conflict	148	1	R → L in AAA51805. Ref.1
	Sequence conflict	148	1	R → L no nucleotide entry Ref.6
	Sequence conflict	248	1	F → L in AAC50873. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14415 [UniParc]. Last modified February 6, 2007. Version 3. Checksum: CC5BFBB6D01347F1	FASTA	290	33,367
	10 20 30 40 50 60 MVIQKEKKSC GQVVEEWKEF VWNPRTHQFM GRTGTSWAFI LLFYLVFYGF LTAMFTLTMW 70 80 90 100 110 120 VMLQTVSDHT PKYQDRLATP GLMIRPKTEN LDVIVNVSDT ESWDQHVQKL NKFLEPYNDS 130 140 150 160 170 180 IQAQKNDVCR PGRYYEQPDN GVLNYPKRAC QFNRTQLGNC SGIGDSTHYG YSTGQPCVFI 190 200 210 220 230 240 KMNRVINFYA GANQSMNVTC AGKRDEDAEN LGNFVMFPAN GNIDLMYFPY YGKKFHVNYT 250 260 270 280 290 QPLVAVKFLN VTPNVEVNVE CRINAANIAT DDERDKFAGR VAFKLRINKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA for the beta 2 subunit isoform of Na+,K(+)-ATPase from human retina." Hernando N., Martin-Vasallo P., Ghosh S., Ghosh P.K., Swaroop A., Coca-Prados M. Biochim. Biophys. Acta 1189:109-111(1994) [PubMed: 8305453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Expression and synthesis of the Na,K-ATPase beta 2 subunit in human retinal pigment epithelium." Ruiz A.C., Bhat S.P., Bok D. Gene 176:237-242(1996) [PubMed: 8918259] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retinal pigment epithelium.
[3]	"Structure and expression of the human Na,K-ATPase beta 2-subunit gene." Avila J., Alvarez de la Rosa D., Gonzalez-Martinez L.M., Lecuona E., Martin-Vasallo P. Gene 208:221-227(1998) [PubMed: 9524271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus.
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"Identification of a putative isoform of the Na,K-ATPase beta subunit. Primary structure and tissue-specific expression." Martin-Vasallo P., Dackowski W., Emanuel J.R., Levenson R. J. Biol. Chem. 264:4613-4618(1989) [PubMed: 2538450] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-290.
[8]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-238, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M81181 mRNA. Translation: AAA51805.1. U45945 mRNA. Translation: AAC50873.1. AF007876 Genomic DNA. Translation: AAC39686.1. AK290143 mRNA. Translation: BAF82832.1. CH471108 Genomic DNA. Translation: EAW90148.1. BC126175 mRNA. Translation: AAI26176.1.
IPI	IPI00293971.
PIR	B32459. JC5107.
RefSeq	NP_001669.3. NM_001678.3.
UniGene	Hs.643540.
3D structure databases
ProteinModelPortal	P14415.
SMR	P14415. Positions 23-289.
ModBase	Search...
Protein-protein interaction databases
STRING	P14415.
PTM databases
PhosphoSite	P14415.
Polymorphism databases
DMDM	125987795.
Proteomic databases
PRIDE	P14415.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000250111; ENSP00000250111; ENSG00000129244.
GeneID	482.
KEGG	hsa:482.
UCSC	uc002gif.1. human.
Organism-specific databases
CTD	482.
GeneCards	GC17P007554.
H-InvDB	HIX0039182.
HGNC	HGNC:805. ATP1B2.
HPA	HPA010698.
MIM	182331. gene.
neXtProt	NX_P14415.
PharmGKB	PA67.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05505.
HOGENOM	HBG446583.
HOVERGEN	HBG050603.
InParanoid	P14415.
OMA	TCVGKRD.
OrthoDB	EOG46144C.
PhylomeDB	P14415.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P14415.
Bgee	P14415.
CleanEx	HS_ATP1B2.
Genevestigator	P14415.
GermOnline	ENSG00000129244. Homo sapiens.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	2001.
SOURCE	Search...

Entry information

Entry name

AT1B2_HUMAN

Accession

Primary (citable) accession number: P14415
Secondary accession number(s): A0AV17 , A8K278, D3DTQ2, O60444

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 110 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.