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P14408

- FUMH_RAT

UniProt

P14408 - FUMH_RAT

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Protein

Fumarate hydratase, mitochondrial

Gene

Fh

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate = fumarate + H2O.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei144 – 1441SubstrateBy similarity

GO - Molecular functioni

  1. fumarate hydratase activity Source: RGD

GO - Biological processi

  1. fumarate metabolic process Source: RGD
  2. malate metabolic process Source: RGD
  3. tricarboxylic acid cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

SABIO-RKP14408.
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase, mitochondrial (EC:4.2.1.2)
Short name:
Fumarase
Gene namesi
Name:Fh
Synonyms:Fh1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2614. Fh.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrion Source: RGD
  2. tricarboxylic acid cycle enzyme complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4141MitochondrionAdd
BLAST
Chaini42 – 507466Fumarate hydratase, mitochondrialPRO_0000010325Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei58 – 581N6-acetyllysine; alternateBy similarity
Modified residuei58 – 581N6-succinyllysine; alternateBy similarity
Modified residuei63 – 631N6-acetyllysine; alternateBy similarity
Modified residuei63 – 631N6-succinyllysine; alternateBy similarity
Modified residuei77 – 771N6-acetyllysine; alternateBy similarity
Modified residuei77 – 771N6-succinyllysine; alternateBy similarity
Modified residuei91 – 911N6-acetyllysineBy similarity
Modified residuei112 – 1121N6-acetyllysine; alternateBy similarity
Modified residuei112 – 1121N6-succinyllysine; alternateBy similarity
Modified residuei119 – 1191N6-acetyllysine; alternateBy similarity
Modified residuei119 – 1191N6-succinyllysine; alternateBy similarity
Modified residuei210 – 2101N6-acetyllysineBy similarity
Modified residuei220 – 2201N6-acetyllysine; alternateBy similarity
Modified residuei220 – 2201N6-succinyllysine; alternateBy similarity
Modified residuei289 – 2891N6-acetyllysine; alternateBy similarity
Modified residuei289 – 2891N6-succinyllysine; alternateBy similarity
Modified residuei464 – 4641N6-succinyllysineBy similarity
Modified residuei470 – 4701N6-succinyllysineBy similarity
Modified residuei499 – 4991N6-acetyllysineBy similarity

Post-translational modificationi

Isoform Cytoplasmic is acetylated at position 2.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP14408.
PRIDEiP14408.

PTM databases

PhosphoSiteiP14408.

Expressioni

Gene expression databases

GenevestigatoriP14408.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

IntActiP14408. 1 interaction.
STRINGi10116.ENSRNOP00000004917.

Structurei

3D structure databases

ProteinModelPortaliP14408.
SMRiP14408. Positions 46-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni173 – 1764B siteBy similarity
Regioni183 – 1853Substrate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0114.
HOGENOMiHOG000061736.
HOVERGENiHBG002183.
InParanoidiP14408.
PhylomeDBiP14408.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform Mitochondrial (identifier: P14408-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRAFCLLAR SRRFPRVPSA GAVLSGEAAT LPRCAPNVVR MASQNSFRIE
60 70 80 90 100
YDTFGELKVP TDKYYGAQTV RSTMNFKIGG ATERMPIPVI KAFGILKRAA
110 120 130 140 150
AEVNQEYGLD PKIASAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV
160 170 180 190 200
NEVISNRAIE MLGGELGSKK PVHPNDHVNK SQSSNDTFPT AMHIAAALEV
210 220 230 240 250
HQVLLPGLQK LHDALSAKSK EFAQVIKIGR THTQDAVPLT LGQEFSGYVQ
260 270 280 290 300
QVQYAMERIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL
310 320 330 340 350
PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL
360 370 380 390 400
GELILPENEP GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF
410 420 430 440 450
ELNVFKPMMI KNVLHSARLL GDASVSFTEN CVVGIQANTE RINKLMNESL
460 470 480 490 500
MLVTALNPHI GYDKAAKIAK TAHKNGSTLK KTAIELGYLT AEQFDEWVKP

KDMLGPK
Length:507
Mass (Da):54,464
Last modified:January 1, 1990 - v1
Checksum:i0304255E04C0D7D8
GO
Isoform Cytoplasmic (identifier: P14408-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2 (By similarity).By similarity

Show »
Length:467
Mass (Da):50,170
Checksum:i33CC61C8CC024663
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform Cytoplasmic. CuratedVSP_018968Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04473 mRNA. Translation: AAA41177.1.
PIRiA31424. UFRT.
UniGeneiRn.29782.

Genome annotation databases

UCSCiRGD:2614. rat. [P14408-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J04473 mRNA. Translation: AAA41177.1 .
PIRi A31424. UFRT.
UniGenei Rn.29782.

3D structure databases

ProteinModelPortali P14408.
SMRi P14408. Positions 46-507.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P14408. 1 interaction.
STRINGi 10116.ENSRNOP00000004917.

PTM databases

PhosphoSitei P14408.

Proteomic databases

PaxDbi P14408.
PRIDEi P14408.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2614. rat. [P14408-1 ]

Organism-specific databases

RGDi 2614. Fh.

Phylogenomic databases

eggNOGi COG0114.
HOGENOMi HOG000061736.
HOVERGENi HBG002183.
InParanoidi P14408.
PhylomeDBi P14408.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER01007 .
SABIO-RK P14408.

Miscellaneous databases

PROi P14408.

Gene expression databases

Genevestigatori P14408.

Family and domain databases

Gene3Di 1.10.275.10. 1 hit.
HAMAPi MF_00743. FumaraseC.
InterProi IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view ]
PANTHERi PTHR11444. PTHR11444. 1 hit.
Pfami PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view ]
PRINTSi PR00149. FUMRATELYASE.
SUPFAMi SSF48557. SSF48557. 1 hit.
TIGRFAMsi TIGR00979. fumC_II. 1 hit.
PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single gene."
    Suzuki T., Sato M., Yoshida T., Tuboi S.
    J. Biol. Chem. 264:2581-2586(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. Lubec G., Afjehi-Sadat L., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 266-283 AND 420-441, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus and Spinal cord.

Entry informationi

Entry nameiFUMH_RAT
AccessioniPrimary (citable) accession number: P14408
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 1, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3