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P14408 (FUMH_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase, mitochondrial

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:Fh
Synonyms:Fh1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length507 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate = fumarate + H2O. HAMAP-Rule MF_00743

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP-Rule MF_00743

Subunit structure

Homotetramer.

Subcellular location

Isoform Mitochondrial: Mitochondrion HAMAP-Rule MF_00743.

Isoform Cytoplasmic: Cytoplasm HAMAP-Rule MF_00743.

Post-translational modification

Isoform Cytoplasmic is acetylated at position 2 By similarity. HAMAP-Rule MF_00743

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.

Sequence similarities

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Mitochondrial (identifier: P14408-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Cytoplasmic (identifier: P14408-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.
Note: Initiator Met-1 is removed. Contains a N-acetylalanine at position 2 (By similarity).

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4141Mitochondrion HAMAP-Rule MF_00743
Chain42 – 507466Fumarate hydratase, mitochondrial HAMAP-Rule MF_00743
PRO_0000010325

Regions

Region173 – 1764B site By similarity
Region183 – 1853Substrate binding By similarity

Sites

Binding site1441Substrate By similarity

Amino acid modifications

Modified residue581N6-acetyllysine; alternate By similarity
Modified residue581N6-succinyllysine; alternate By similarity
Modified residue631N6-acetyllysine; alternate By similarity
Modified residue631N6-succinyllysine; alternate By similarity
Modified residue771N6-acetyllysine; alternate By similarity
Modified residue771N6-succinyllysine; alternate By similarity
Modified residue911N6-acetyllysine By similarity
Modified residue1121N6-acetyllysine; alternate By similarity
Modified residue1121N6-succinyllysine; alternate By similarity
Modified residue1191N6-acetyllysine; alternate By similarity
Modified residue1191N6-succinyllysine; alternate By similarity
Modified residue2101N6-acetyllysine By similarity
Modified residue2201N6-acetyllysine; alternate By similarity
Modified residue2201N6-succinyllysine; alternate By similarity
Modified residue2891N6-acetyllysine; alternate By similarity
Modified residue2891N6-succinyllysine; alternate By similarity
Modified residue4641N6-succinyllysine By similarity
Modified residue4701N6-succinyllysine By similarity
Modified residue4991N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 4040Missing in isoform Cytoplasmic.
VSP_018968

Sequences

Sequence LengthMass (Da)Tools
Isoform Mitochondrial [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 0304255E04C0D7D8

FASTA50754,464
        10         20         30         40         50         60 
MNRAFCLLAR SRRFPRVPSA GAVLSGEAAT LPRCAPNVVR MASQNSFRIE YDTFGELKVP 

        70         80         90        100        110        120 
TDKYYGAQTV RSTMNFKIGG ATERMPIPVI KAFGILKRAA AEVNQEYGLD PKIASAIMKA 

       130        140        150        160        170        180 
ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKK PVHPNDHVNK 

       190        200        210        220        230        240 
SQSSNDTFPT AMHIAAALEV HQVLLPGLQK LHDALSAKSK EFAQVIKIGR THTQDAVPLT 

       250        260        270        280        290        300 
LGQEFSGYVQ QVQYAMERIK AAMPRIYELA AGGTAVGTGL NTRIGFAEKV AAKVAALTGL 

       310        320        330        340        350        360 
PFVTAPNKFE ALAAHDALVE LSGAMNTTAC SLMKIANDIR FLGSGPRSGL GELILPENEP 

       370        380        390        400        410        420 
GSSIMPGKVN PTQCEAMTMV AAQVMGNHVA VTVGGSNGHF ELNVFKPMMI KNVLHSARLL 

       430        440        450        460        470        480 
GDASVSFTEN CVVGIQANTE RINKLMNESL MLVTALNPHI GYDKAAKIAK TAHKNGSTLK 

       490        500 
KTAIELGYLT AEQFDEWVKP KDMLGPK 

« Hide

Isoform Cytoplasmic [UniParc].

Checksum: 33CC61C8CC024663
Show »

FASTA46750,170

References

[1]"Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single gene."
Suzuki T., Sato M., Yoshida T., Tuboi S.
J. Biol. Chem. 264:2581-2586(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Liver.
[2]Lubec G., Afjehi-Sadat L., Chen W.-Q.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 266-283 AND 420-441, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: Sprague-Dawley.
Tissue: Hippocampus and Spinal cord.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04473 mRNA. Translation: AAA41177.1.
PIRUFRT. A31424.
UniGeneRn.29782.

3D structure databases

ProteinModelPortalP14408.
SMRP14408. Positions 46-507.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP14408. 1 interaction.
STRING10116.ENSRNOP00000004917.

PTM databases

PhosphoSiteP14408.

Proteomic databases

PaxDbP14408.
PRIDEP14408.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:2614. rat. [P14408-1]

Organism-specific databases

RGD2614. Fh.

Phylogenomic databases

eggNOGCOG0114.
HOGENOMHOG000061736.
HOVERGENHBG002183.
InParanoidP14408.
PhylomeDBP14408.

Enzyme and pathway databases

SABIO-RKP14408.
UniPathwayUPA00223; UER01007.

Gene expression databases

GenevestigatorP14408.

Family and domain databases

Gene3D1.10.275.10. 1 hit.
HAMAPMF_00743. FumaraseC.
InterProIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERPTHR11444. PTHR11444. 1 hit.
PfamPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSPR00149. FUMRATELYASE.
SUPFAMSSF48557. SSF48557. 1 hit.
TIGRFAMsTIGR00979. fumC_II. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP14408.

Entry information

Entry nameFUMH_RAT
AccessionPrimary (citable) accession number: P14408
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways