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P14407

- FUMB_ECOLI

UniProt

P14407 - FUMB_ECOLI

Protein

Fumarate hydratase class I, anaerobic

Gene

fumB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (19 Jul 2003)
      Previous versions | rss
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    Functioni

    It functions in the generation of fumarate for use as an anaerobic electron acceptor.

    Catalytic activityi

    (S)-malate = fumarate + H2O.

    Cofactori

    Binds 1 4Fe-4S cluster.

    Enzyme regulationi

    Subject to anaerobic repression.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi318 – 3181Iron-sulfur (4Fe-4S)By similarity
    Active sitei397 – 3971Sequence Analysis
    Binding sitei463 – 4631SubstrateSequence Analysis

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: EcoCyc
    2. D(-)-tartrate dehydratase activity Source: EcoCyc
    3. fumarate hydratase activity Source: EcoCyc
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. biofilm formation Source: EcoCyc
    2. cellular response to DNA damage stimulus Source: EcoliWiki
    3. tricarboxylic acid cycle Source: EcoCyc

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:FUMB-MONOMER.
    ECOL316407:JW4083-MONOMER.
    MetaCyc:FUMB-MONOMER.
    SABIO-RKP14407.
    UniPathwayiUPA00223; UER01007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fumarate hydratase class I, anaerobic (EC:4.2.1.2)
    Short name:
    Fumarase
    Gene namesi
    Name:fumB
    Ordered Locus Names:b4122, JW4083
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10357. fumB.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 548548Fumarate hydratase class I, anaerobicPRO_0000195660Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei192 – 1921N6-acetyllysine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP14407.
    PRIDEiP14407.

    Expressioni

    Gene expression databases

    GenevestigatoriP14407.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP14407. 6 interactions.
    STRINGi511145.b4122.

    Structurei

    3D structure databases

    ProteinModelPortaliP14407.
    SMRiP14407. Positions 356-537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the class-I fumarase family.Curated

    Phylogenomic databases

    eggNOGiCOG1951.
    HOGENOMiHOG000009338.
    KOiK01676.
    OMAiDSEGVNA.
    OrthoDBiEOG6TXR10.
    PhylomeDBiP14407.

    Family and domain databases

    Gene3Di3.20.130.10. 1 hit.
    InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view]
    PfamiPF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMiSSF117457. SSF117457. 1 hit.
    TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14407-1 [UniParc]FASTAAdd to Basket

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    MSNKPFIYQA PFPMGKDNTE YYLLTSDYVS VADFDGETIL KVEPEALTLL    50
    AQQAFHDASF MLRPAHQKQV AAILHDPEAS ENDKYVALQF LRNSEIAAKG 100
    VLPTCQDTGT AIIVGKKGQR VWTGGGDEET LSKGVYNTYI EDNLRYSQNA 150
    ALDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCVAKGGGSA NKTYLYQETK 200
    ALLTPGKLKN FLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA 250
    HYYDELPTEG NEHGQAFRDV QLEQELLEEA QKLGLGAQFG GKYFAHDIRV 300
    IRLPRHGASC PVGMGVSCSA DRNIKAKINR EGIWIEKLEH NPGQYIPQEL 350
    RQAGEGEAVK VDLNRPMKEI LAQLSQYPVS TRLSLTGTII VGRDIAHAKL 400
    KELIDAGKEL PQYIKDHPIY YAGPAKTPAG YPSGSLGPTT AGRMDSYVDL 450
    LQSHGGSMIM LAKGNRSQQV TDACHKHGGF YLGSIGGPAA VLAQQSIKHL 500
    ECVAYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIVN KQCANCTK 548
    Length:548
    Mass (Da):60,105
    Last modified:July 19, 2003 - v2
    Checksum:i73685337862496B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti50 – 501L → V in AAA23827. (PubMed:2656658)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27058 Genomic DNA. Translation: AAA23827.1.
    U14003 Genomic DNA. Translation: AAA97022.1.
    U00096 Genomic DNA. Translation: AAC77083.1.
    AP009048 Genomic DNA. Translation: BAE78124.1.
    PIRiA65222. B44511.
    RefSeqiNP_418546.1. NC_000913.3.
    YP_492265.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77083; AAC77083; b4122.
    BAE78124; BAE78124; BAE78124.
    GeneIDi12933156.
    948642.
    KEGGiecj:Y75_p4009.
    eco:b4122.
    PATRICi32123809. VBIEscCol129921_4253.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M27058 Genomic DNA. Translation: AAA23827.1 .
    U14003 Genomic DNA. Translation: AAA97022.1 .
    U00096 Genomic DNA. Translation: AAC77083.1 .
    AP009048 Genomic DNA. Translation: BAE78124.1 .
    PIRi A65222. B44511.
    RefSeqi NP_418546.1. NC_000913.3.
    YP_492265.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P14407.
    SMRi P14407. Positions 356-537.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P14407. 6 interactions.
    STRINGi 511145.b4122.

    Proteomic databases

    PaxDbi P14407.
    PRIDEi P14407.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77083 ; AAC77083 ; b4122 .
    BAE78124 ; BAE78124 ; BAE78124 .
    GeneIDi 12933156.
    948642.
    KEGGi ecj:Y75_p4009.
    eco:b4122.
    PATRICi 32123809. VBIEscCol129921_4253.

    Organism-specific databases

    EchoBASEi EB0352.
    EcoGenei EG10357. fumB.

    Phylogenomic databases

    eggNOGi COG1951.
    HOGENOMi HOG000009338.
    KOi K01676.
    OMAi DSEGVNA.
    OrthoDBi EOG6TXR10.
    PhylomeDBi P14407.

    Enzyme and pathway databases

    UniPathwayi UPA00223 ; UER01007 .
    BioCyci EcoCyc:FUMB-MONOMER.
    ECOL316407:JW4083-MONOMER.
    MetaCyc:FUMB-MONOMER.
    SABIO-RK P14407.

    Miscellaneous databases

    PROi P14407.

    Gene expression databases

    Genevestigatori P14407.

    Family and domain databases

    Gene3Di 3.20.130.10. 1 hit.
    InterProi IPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
    IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
    IPR011167. Fe_dep_fumarate_hydratase.
    IPR020557. Fumarate_lyase_CS.
    [Graphical view ]
    Pfami PF05681. Fumerase. 1 hit.
    PF05683. Fumerase_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001394. Fe_dep_fumar_hy. 1 hit.
    SUPFAMi SSF117457. SSF117457. 1 hit.
    TIGRFAMsi TIGR00722. ttdA_fumA_fumB. 1 hit.
    TIGR00723. ttdB_fumA_fumB. 1 hit.
    PROSITEi PS00163. FUMARATE_LYASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12."
      Bell P.J., Andrews S.C., Sivak M.N., Guest J.R.
      J. Bacteriol. 171:3494-3503(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 50.
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
      Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
      Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-192, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiFUMB_ECOLI
    AccessioniPrimary (citable) accession number: P14407
    Secondary accession number(s): P78139, Q2M6I2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: July 19, 2003
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3