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Protein

Fumarate hydratase class I, anaerobic

Gene

fumB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible hydration of fumarate to (S)-malate. Functions in the generation of fumarate for use as an anaerobic electron acceptor. To a lesser extent, also displays D-tartrate dehydratase activity, but is not able to convert (R)-malate, L-tartrate or meso-tartrate. Is required for anaerobic growth on D-tartrate.4 Publications

Catalytic activityi

(S)-malate = fumarate + H2O.1 Publication
(S,S)-tartrate = oxaloacetate + H2O.1 Publication

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Kineticsi

  1. KM=300 µM for (S)-malate1 Publication
  2. KM=320 µM for fumarate1 Publication
  3. KM=800 µM for D-tartrate1 Publication
  1. Vmax=490 µmol/min/mg enzyme for (S)-malate dehydration1 Publication
  2. Vmax=1430 µmol/min/mg enzyme for fumarate hydration1 Publication
  3. Vmax=9.2 µmol/min/mg enzyme for D-tartrate dehydration1 Publication

GO - Molecular functioni

  • 4 iron, 4 sulfur cluster binding Source: EcoCyc
  • D(-)-tartrate dehydratase activity Source: EcoCyc
  • fumarate hydratase activity Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • cellular response to DNA damage stimulus Source: EcoliWiki
  • single-species biofilm formation Source: EcoCyc
  • tricarboxylic acid cycle Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:FUMB-MONOMER.
ECOL316407:JW4083-MONOMER.
MetaCyc:FUMB-MONOMER.
SABIO-RKP14407.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class I, anaerobic1 Publication (EC:4.2.1.21 Publication)
Alternative name(s):
D-tartrate dehydratase1 Publication (EC:4.2.1.811 Publication)
Fumarase B1 Publication
Gene namesi
Name:fumB1 Publication
Ordered Locus Names:b4122, JW4083
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10357. fumB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Disruption of this gene seriously impairs growth under anaerobic conditions on D-tartrate when glycerol is supplied as an electron donor (D-tartrate fermentation). Cells lacking this gene also lose most (about 79%) of D-tartrate dehydratase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001956601 – 548Fumarate hydratase class I, anaerobicAdd BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei192N6-acetyllysine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP14407.
PRIDEiP14407.

Expressioni

Inductioni

Is mainly expressed during anaerobic growth. Is under the control of the Fnr transcriptional regulator.1 Publication

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

BioGridi4262150. 8 interactors.
IntActiP14407. 6 interactors.
STRINGi511145.b4122.

Structurei

3D structure databases

ProteinModelPortaliP14407.
SMRiP14407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-I fumarase family.Curated

Phylogenomic databases

eggNOGiENOG4107GFK. Bacteria.
COG1838. LUCA.
COG1951. LUCA.
HOGENOMiHOG000009338.
InParanoidiP14407.
KOiK01676.
OMAiWAPNTET.
PhylomeDBiP14407.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14407-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKPFIYQA PFPMGKDNTE YYLLTSDYVS VADFDGETIL KVEPEALTLL
60 70 80 90 100
AQQAFHDASF MLRPAHQKQV AAILHDPEAS ENDKYVALQF LRNSEIAAKG
110 120 130 140 150
VLPTCQDTGT AIIVGKKGQR VWTGGGDEET LSKGVYNTYI EDNLRYSQNA
160 170 180 190 200
ALDMYKEVNT GTNLPAQIDL YAVDGDEYKF LCVAKGGGSA NKTYLYQETK
210 220 230 240 250
ALLTPGKLKN FLVEKMRTLG TAACPPYHIA FVIGGTSAET NLKTVKLASA
260 270 280 290 300
HYYDELPTEG NEHGQAFRDV QLEQELLEEA QKLGLGAQFG GKYFAHDIRV
310 320 330 340 350
IRLPRHGASC PVGMGVSCSA DRNIKAKINR EGIWIEKLEH NPGQYIPQEL
360 370 380 390 400
RQAGEGEAVK VDLNRPMKEI LAQLSQYPVS TRLSLTGTII VGRDIAHAKL
410 420 430 440 450
KELIDAGKEL PQYIKDHPIY YAGPAKTPAG YPSGSLGPTT AGRMDSYVDL
460 470 480 490 500
LQSHGGSMIM LAKGNRSQQV TDACHKHGGF YLGSIGGPAA VLAQQSIKHL
510 520 530 540
ECVAYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIVN KQCANCTK
Length:548
Mass (Da):60,105
Last modified:July 19, 2003 - v2
Checksum:i73685337862496B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti50L → V in AAA23827 (PubMed:2656658).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27058 Genomic DNA. Translation: AAA23827.1.
U14003 Genomic DNA. Translation: AAA97022.1.
U00096 Genomic DNA. Translation: AAC77083.1.
AP009048 Genomic DNA. Translation: BAE78124.1.
PIRiA65222. B44511.
RefSeqiNP_418546.1. NC_000913.3.
WP_000066707.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77083; AAC77083; b4122.
BAE78124; BAE78124; BAE78124.
GeneIDi948642.
KEGGiecj:JW4083.
eco:b4122.
PATRICi32123809. VBIEscCol129921_4253.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27058 Genomic DNA. Translation: AAA23827.1.
U14003 Genomic DNA. Translation: AAA97022.1.
U00096 Genomic DNA. Translation: AAC77083.1.
AP009048 Genomic DNA. Translation: BAE78124.1.
PIRiA65222. B44511.
RefSeqiNP_418546.1. NC_000913.3.
WP_000066707.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP14407.
SMRiP14407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262150. 8 interactors.
IntActiP14407. 6 interactors.
STRINGi511145.b4122.

Proteomic databases

PaxDbiP14407.
PRIDEiP14407.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77083; AAC77083; b4122.
BAE78124; BAE78124; BAE78124.
GeneIDi948642.
KEGGiecj:JW4083.
eco:b4122.
PATRICi32123809. VBIEscCol129921_4253.

Organism-specific databases

EchoBASEiEB0352.
EcoGeneiEG10357. fumB.

Phylogenomic databases

eggNOGiENOG4107GFK. Bacteria.
COG1838. LUCA.
COG1951. LUCA.
HOGENOMiHOG000009338.
InParanoidiP14407.
KOiK01676.
OMAiWAPNTET.
PhylomeDBiP14407.

Enzyme and pathway databases

BioCyciEcoCyc:FUMB-MONOMER.
ECOL316407:JW4083-MONOMER.
MetaCyc:FUMB-MONOMER.
SABIO-RKP14407.

Miscellaneous databases

PROiP14407.

Family and domain databases

Gene3Di3.20.130.10. 1 hit.
InterProiIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamiPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMiSSF117457. SSF117457. 1 hit.
TIGRFAMsiTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUMB_ECOLI
AccessioniPrimary (citable) accession number: P14407
Secondary accession number(s): P78139, Q2M6I2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 19, 2003
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.