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P14407 (FUMB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Fumarate hydratase class I, anaerobic

Short name=Fumarase
EC=4.2.1.2
Gene names
Name:fumB
Ordered Locus Names:b4122, JW4083
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length548 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

It functions in the generation of fumarate for use as an anaerobic electron acceptor.

Catalytic activity

(S)-malate = fumarate + H2O.

Cofactor

Binds 1 4Fe-4S cluster.

Enzyme regulation

Subject to anaerobic repression.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the class-I fumarase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 548548Fumarate hydratase class I, anaerobic
PRO_0000195660

Sites

Active site3971 Potential
Metal binding3181Iron-sulfur (4Fe-4S) By similarity
Binding site4631Substrate Potential

Amino acid modifications

Modified residue1921N6-acetyllysine Ref.5

Experimental info

Sequence conflict501L → V in AAA23827. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14407 [UniParc].

Last modified July 19, 2003. Version 2.
Checksum: 73685337862496B7

FASTA54860,105
        10         20         30         40         50         60 
MSNKPFIYQA PFPMGKDNTE YYLLTSDYVS VADFDGETIL KVEPEALTLL AQQAFHDASF 

        70         80         90        100        110        120 
MLRPAHQKQV AAILHDPEAS ENDKYVALQF LRNSEIAAKG VLPTCQDTGT AIIVGKKGQR 

       130        140        150        160        170        180 
VWTGGGDEET LSKGVYNTYI EDNLRYSQNA ALDMYKEVNT GTNLPAQIDL YAVDGDEYKF 

       190        200        210        220        230        240 
LCVAKGGGSA NKTYLYQETK ALLTPGKLKN FLVEKMRTLG TAACPPYHIA FVIGGTSAET 

       250        260        270        280        290        300 
NLKTVKLASA HYYDELPTEG NEHGQAFRDV QLEQELLEEA QKLGLGAQFG GKYFAHDIRV 

       310        320        330        340        350        360 
IRLPRHGASC PVGMGVSCSA DRNIKAKINR EGIWIEKLEH NPGQYIPQEL RQAGEGEAVK 

       370        380        390        400        410        420 
VDLNRPMKEI LAQLSQYPVS TRLSLTGTII VGRDIAHAKL KELIDAGKEL PQYIKDHPIY 

       430        440        450        460        470        480 
YAGPAKTPAG YPSGSLGPTT AGRMDSYVDL LQSHGGSMIM LAKGNRSQQV TDACHKHGGF 

       490        500        510        520        530        540 
YLGSIGGPAA VLAQQSIKHL ECVAYPELGM EAIWKIEVED FPAFILVDDK GNDFFQQIVN 


KQCANCTK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12."
Bell P.J., Andrews S.C., Sivak M.N., Guest J.R.
J. Bacteriol. 171:3494-3503(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 50.
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli."
Zhang J., Sprung R., Pei J., Tan X., Kim S., Zhu H., Liu C.F., Grishin N.V., Zhao Y.
Mol. Cell. Proteomics 8:215-225(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-192, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: K12 / JW1106 and K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M27058 Genomic DNA. Translation: AAA23827.1.
U14003 Genomic DNA. Translation: AAA97022.1.
U00096 Genomic DNA. Translation: AAC77083.1.
AP009048 Genomic DNA. Translation: BAE78124.1.
PIRB44511. A65222.
RefSeqNP_418546.1. NC_000913.3.
YP_492265.1. NC_007779.1.

3D structure databases

ProteinModelPortalP14407.
SMRP14407. Positions 356-537.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP14407. 6 interactions.
STRING511145.b4122.

Proteomic databases

PaxDbP14407.
PRIDEP14407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77083; AAC77083; b4122.
BAE78124; BAE78124; BAE78124.
GeneID12933156.
948642.
KEGGecj:Y75_p4009.
eco:b4122.
PATRIC32123809. VBIEscCol129921_4253.

Organism-specific databases

EchoBASEEB0352.
EcoGeneEG10357. fumB.

Phylogenomic databases

eggNOGCOG1951.
HOGENOMHOG000009338.
KOK01676.
OMAKVECIDF.
OrthoDBEOG6TXR10.
ProtClustDBPRK15391.

Enzyme and pathway databases

BioCycEcoCyc:FUMB-MONOMER.
ECOL316407:JW4083-MONOMER.
MetaCyc:FUMB-MONOMER.
SABIO-RKP14407.
UniPathwayUPA00223; UER01007.

Gene expression databases

GenevestigatorP14407.

Family and domain databases

Gene3D3.20.130.10. 1 hit.
InterProIPR004646. Fe-S_hydro-lyase_TtdA-typ_cat.
IPR004647. Fe-S_hydro-lyase_TtdB-typ_cat.
IPR011167. Fe_dep_fumarate_hydratase.
IPR020557. Fumarate_lyase_CS.
[Graphical view]
PfamPF05681. Fumerase. 1 hit.
PF05683. Fumerase_C. 1 hit.
[Graphical view]
PIRSFPIRSF001394. Fe_dep_fumar_hy. 1 hit.
SUPFAMSSF117457. SSF117457. 1 hit.
TIGRFAMsTIGR00722. ttdA_fumA_fumB. 1 hit.
TIGR00723. ttdB_fumA_fumB. 1 hit.
PROSITEPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP14407.

Entry information

Entry nameFUMB_ECOLI
AccessionPrimary (citable) accession number: P14407
Secondary accession number(s): P78139, Q2M6I2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 19, 2003
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene