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Protein

MDS1 and EVI1 complex locus protein EVI1

Gene

Mecom

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a transcriptional regulator binding to DNA sequences in the promoter region of target genes and regulating positively or negatively their expression. Oncogene which plays a role in development, cell proliferation and differentiation. May also play a role in apoptosis through regulation of the JNK and TGF-beta signaling. Involved in hematopoiesis.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 44C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri75 – 97C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri103 – 125C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri131 – 154C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri160 – 182C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri188 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri217 – 239C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri724 – 746C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri752 – 775C2H2-type 9PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri781 – 803C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: UniProtKB
  • transcription factor activity, sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  • apoptotic process Source: UniProtKB-KW
  • cell differentiation Source: UniProtKB-KW
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic hindlimb morphogenesis Source: MGI
  • forebrain development Source: MGI
  • hematopoietic stem cell proliferation Source: UniProtKB
  • inflammatory response Source: MGI
  • in utero embryonic development Source: MGI
  • maintenance of cell number Source: MGI
  • negative regulation of JNK cascade Source: UniProtKB
  • negative regulation of programmed cell death Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • neutrophil homeostasis Source: MGI
  • pericardium development Source: MGI
  • positive regulation of brown fat cell differentiation Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • post-embryonic development Source: MGI
  • regulation of cell cycle Source: UniProtKB
  • regulation of cell proliferation Source: MGI
  • renal system development Source: MGI
  • response to bacterium Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Apoptosis, Differentiation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Names & Taxonomyi

Protein namesi
Recommended name:
MDS1 and EVI1 complex locus protein EVI1
Alternative name(s):
Ecotropic virus integration site 1 protein
Short name:
EVI-1
Gene namesi
Name:Mecom
Synonyms:Evi1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:95457. Mecom.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop until 9.5 dpc but die before 11.5 dpc. At 10.5 dpc embryos display multiple malformations associated with hypocellularity and reduced body size. Required for neural, heart and paraxial mesenchyme.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000472741 – 1042MDS1 and EVI1 complex locus protein EVI1Add BLAST1042

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki2Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki367Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei436PhosphoserineCombined sources1
Cross-linki497Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki533Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei538PhosphoserineBy similarity1
Cross-linki547Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei552PhosphoserineCombined sources1
Cross-linki564Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki599Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki689Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei849PhosphoserineBy similarity1
Modified residuei851PhosphoserineCombined sources1
Cross-linki865Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki939Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki944Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationi

May be acetylated by CREBBP and KAT2B.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP14404.
PRIDEiP14404.

PTM databases

iPTMnetiP14404.
PhosphoSitePlusiP14404.

Expressioni

Developmental stagei

Expressed at 8.5 dpc in the anterior section of the primary head folds. Ubiquitously expressed at 9.5 dpc with higher expression in forebrain, mesenchyme of the branchial arches, nasal pits, limb buds and mesonephric ducts. Also detected at 10.5 dpc in hindbrain and lateral region of the neural tube.1 Publication

Gene expression databases

BgeeiENSMUSG00000027684.
CleanExiMM_EVI1.
ExpressionAtlasiP14404. baseline and differential.
GenevisibleiP14404. MM.

Interactioni

Subunit structurei

Homooligomer. Interacts with CTBP1. Interacts with SMAD3 (via MH2 domain); the interaction is direct. Interacts with SMAD4; through interaction with SMAD3. Interacts with CREBBP, KAT2B and histone deacetylases. Interacts with MAPK8 and MAPK9; inhibits JNK signaling (By similarity). Interacts with SUV39H1 (via SET domain); enhances MECOM transcriptional repression activity.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mbd3Q9Z2D84EBI-1994523,EBI-1994548
Mbd3Q9Z2D8-15EBI-1994523,EBI-1994598

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-46516N.
IntActiP14404. 2 interactors.
MINTiMINT-197580.
STRINGi10090.ENSMUSP00000134303.

Structurei

3D structure databases

ProteinModelPortaliP14404.
SMRiP14404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 252Interaction with SUV39H1 and probably MAPK9 and SMAD31 PublicationAdd BLAST252

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi421 – 434Nuclear localization signalSequence analysisAdd BLAST14
Motifi553 – 557CTBP-binding motif 1By similarity5
Motifi584 – 588CTBP-binding motif 2By similarity5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi877 – 928Asp/Glu-rich (acidic)Add BLAST52

Domaini

Both zinc finger regions are required for the transcriptional activation of PBX1.By similarity

Sequence similaritiesi

Contains 10 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 44C2H2-type 1PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri75 – 97C2H2-type 2PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri103 – 125C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri131 – 154C2H2-type 4PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri160 – 182C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri188 – 210C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri217 – 239C2H2-type 7PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri724 – 746C2H2-type 8PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri752 – 775C2H2-type 9PROSITE-ProRule annotationAdd BLAST24
Zinc fingeri781 – 803C2H2-type 10PROSITE-ProRule annotationAdd BLAST23

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063676.
HOGENOMiHOG000231144.
HOVERGENiHBG005619.
InParanoidiP14404.
PhylomeDBiP14404.
TreeFamiTF315309.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR030413. Evi1/Prdm16.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR24393. PTHR24393. 1 hit.
PfamiPF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 10 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage and alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P14404-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSEEDPHEP MAPDIHEERQ HRCEDCDQLF ESKAELADHQ KFPCSTPHSA
60 70 80 90 100
FSMVEEDLQQ NLESESDLRE IHGNQDCKEC DRVFPDLQSL EKHMLSHTEE
110 120 130 140 150
REYKCDQCPK AFNWKSNLIR HQMSHDSGKH YECENCAKVF TDPSNLQRHI
160 170 180 190 200
RSQHVGARAH ACPECGKTFA TSSGLKQHKH IHSSVKPFIC EVCHKSYTQF
210 220 230 240 250
SNLCRHKRMH ADCRTQIKCK DCGQMFSTTS SLNKHRRFCE GKNHFAAGGF
260 270 280 290 300
FGQGISLPGT PAMDKTSMVN MSHANPGLAD YFGTNRHPAG LTFPTAPGFS
310 320 330 340 350
FSFPGLFPSG LYHRPPLIPA SPPVKGLSST EQSNKCQSPL LTHPQILPAT
360 370 380 390 400
QDILKALSKH PPVGDNKPVE LLPERSSEER PLEKISDQSE SSDLDDVSTP
410 420 430 440 450
SGSDLETTSG SDLESDLESD KEKCKENGKM FKDKVSPLQN LASITNKKEH
460 470 480 490 500
NNHSVFSASV EEQSAVSGAV NDSIKAIASI AEKYFGSTGL VGLQDKKVGA
510 520 530 540 550
LPYPSMFPLP FFPAFSQSMY PFPDRDLRSL PLKMEPQSPS EVKKLQKGSS
560 570 580 590 600
ESPFDLTTKR KDEKPLTSGP SKPSGTPATS QDQPLDLSMG SRGRASGTKL
610 620 630 640 650
TEPRKNHVFG EKKGSNMDTR PSSDGSLQHA RPTPFFMDPI YRVEKRKLTD
660 670 680 690 700
PLEALKEKYL RPSPGFLFHP QMSAIENMAE KLESFSALKP EASELLQSVP
710 720 730 740 750
SMFSFRAPPN TLPENLLRKG KERYTCRYCG KIFPRSANLT RHLRTHTGEQ
760 770 780 790 800
PYRCKYCDRS FSISSNLQRH VRNIHNKEKP FKCHLCDRCF GQQTNLDRHL
810 820 830 840 850
KKHENGNMSG TATSSPHSEL ESAGAILDDK EDAYFTEIRN FIGNSNHGSQ
860 870 880 890 900
SPRNMEERMN GSHFKDKKAL ATSQNSDLLD DEEVEDEVLL DEEDEDNDIP
910 920 930 940 950
GKPRKELGVT RLDEEIPEDD YEEAGALEMS CKASPVRYKE EDYKSGLSAL
960 970 980 990 1000
DHIRHFTDSL KMREMEENQY TDAELSSISS SHVPEELKQT LHRKSKSQAY
1010 1020 1030 1040
AMMLSLSDKD SLHPTSHSSS NVWHSMARAA AESSAIQSIS HV
Length:1,042
Mass (Da):116,848
Last modified:January 1, 1990 - v1
Checksum:i8DEDF164F536D2FE
GO
Isoform 2 (identifier: Q9Z1L8-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry Q9Z1L8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by alternative promoter usage. May be due to intron retention.
Length:129
Mass (Da):14,329
GO

Sequence cautioni

The sequence AAA40581 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21829 Genomic DNA. Translation: AAA40581.1. Different initiation.
PIRiA31591.
UniGeneiMm.426829.
Mm.56965.

Genome annotation databases

EnsembliENSMUST00000108270; ENSMUSP00000103905; ENSMUSG00000027684. [P14404-1]
UCSCiuc033hsp.1. mouse. [P14404-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21829 Genomic DNA. Translation: AAA40581.1. Different initiation.
PIRiA31591.
UniGeneiMm.426829.
Mm.56965.

3D structure databases

ProteinModelPortaliP14404.
SMRiP14404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46516N.
IntActiP14404. 2 interactors.
MINTiMINT-197580.
STRINGi10090.ENSMUSP00000134303.

PTM databases

iPTMnetiP14404.
PhosphoSitePlusiP14404.

Proteomic databases

PaxDbiP14404.
PRIDEiP14404.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000108270; ENSMUSP00000103905; ENSMUSG00000027684. [P14404-1]
UCSCiuc033hsp.1. mouse. [P14404-1]

Organism-specific databases

MGIiMGI:95457. Mecom.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00530000063676.
HOGENOMiHOG000231144.
HOVERGENiHBG005619.
InParanoidiP14404.
PhylomeDBiP14404.
TreeFamiTF315309.

Enzyme and pathway databases

ReactomeiR-MMU-3214841. PKMTs methylate histone lysines.

Miscellaneous databases

ChiTaRSiMecom. mouse.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027684.
CleanExiMM_EVI1.
ExpressionAtlasiP14404. baseline and differential.
GenevisibleiP14404. MM.

Family and domain databases

Gene3Di3.30.160.60. 9 hits.
InterProiIPR030413. Evi1/Prdm16.
IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PANTHERiPTHR24393. PTHR24393. 1 hit.
PfamiPF00096. zf-C2H2. 3 hits.
PF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 10 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 8 hits.
PS50157. ZINC_FINGER_C2H2_2. 10 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEVI1_MOUSE
AccessioniPrimary (citable) accession number: P14404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.