Reviewed, UniProtKB/Swiss-Prot P14403 (POLG_JAEVN)

Names and origin

Protein names	Recommended name:     Genome polyprotein Cleaved into the following 7 chains:     1- Recommended name:             Protein C         Alternative name(s):             Core protein             Capsid protein     2- Recommended name:             Small envelope protein M         Alternative name(s):             Matrix protein     3- Recommended name:             Envelope protein E     4- Recommended name:             Non-structural protein 1                 Short name=NS1     5- Recommended name:             Non-structural protein 2A                 Short name=NS2A     6- Recommended name:             Flavivirin protease NS2B regulatory subunit     7- Recommended name:             Flavivirin protease NS3 catalytic subunit               EC=3.4.21.91
Organism	Japanese encephalitis virus (strain Nakayama)
Taxonomic identifier	11076 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › Japanese encephalitis virus group
Virus host	Culex tritaeniorhynchus (Mosquito) [TaxID: 7178] Ardeidae (herons) [TaxID: 8899] Homo sapiens (Human) [TaxID: 9606] Equus caballus (Horse) [TaxID: 9796] Sus scrofa (Pig) [TaxID: 9823] Bos taurus (Bovine) [TaxID: 9913] Culex gelidus [TaxID: 308713]

Protein attributes

Sequence length	1440 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. NS5 may play a role in the viral RNA replication. The NS2B/NS3 protease complex processes the viral polyprotein.
Catalytic activity	Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.
Subunit structure	NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter By similarity.
Subcellular location	Protein C: Virion Potential. Host membrane; Single-pass membrane protein Potential. Small envelope protein M: Virion Potential. Host membrane; Single-pass membrane protein Potential. Envelope protein E: Virion Potential. Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins By similarity.
Miscellaneous	The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E By similarity.

Ontologies

Keywords
Cellular component	Capsid protein Core protein Envelope protein Membrane Virion
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 53	›53	Protein C		PRO_0000037869
	Propeptide	54 – 146	93			PRO_0000037870
	Chain	147 – 222	76	Small envelope protein M		PRO_0000037871
	Chain	223 – 794	572	Envelope protein E		PRO_0000037872
	Chain	795 – 1136	342	Non-structural protein 1		PRO_0000037873
	Chain	1137 – 1301	165	Non-structural protein 2A		PRO_0000037874
	Chain	1302 – 1432	131	Flavivirin protease NS2B regulatory subunit		PRO_0000037875
	Chain	1433 – ›1440	›8	Flavivirin protease NS3 catalytic subunit		PRO_0000037876
Regions
	Transmembrane	36 – 56	21	Potential
	Transmembrane	181 – 201	21	Potential
	Transmembrane	208 – 224	17	Potential
	Transmembrane	675 – 695	21	Potential
	Transmembrane	702 – 722	21	Potential
	Transmembrane	1106 – 1126	21	Potential
	Transmembrane	1148 – 1168	21	Potential
	Transmembrane	1179 – 1199	21	Potential
	Transmembrane	1201 – 1221	21	Potential
	Transmembrane	1238 – 1258	21	Potential
	Transmembrane	1270 – 1290	21	Potential
	Transmembrane	1303 – 1323	21	Potential
	Transmembrane	1327 – 1347	21	Potential
	Transmembrane	1405 – 1425	21	Potential
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	376	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	852	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	929	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	225 ↔ 252		By similarity
	Disulfide bond	282 ↔ 338		By similarity
	Disulfide bond	296 ↔ 327		By similarity
	Disulfide bond	314 ↔ 343		By similarity
	Disulfide bond	412 ↔ 509		By similarity
	Disulfide bond	526 ↔ 557		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	1440	1

Sequences

	Sequence		Length	Mass (Da)
	P14403-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 4D489A365A3C2E6E	FASTA	1,440	158,185
	10 20 30 40 50 60 SVAMKHLTSF KRELGTLIDA VNKRGRKQNK RGGNEGSIMW LASLAVVIAC AGAMKLSNFQ 70 80 90 100 110 120 GKLLMTVNNT DIADVIVIPN PSKGENRCWV RAIDVGYMCE DTITYECPKL TMGNDPEDVD 130 140 150 160 170 180 CWCDNQEVYV QYGRCTRTRH SKRSRRSVSV QTHGESSLVN KKEAWLDSTK ATRYLMKTEN 190 200 210 220 230 240 WIVRNPGYAF LAAILGWMLG SNNGQRRWYF TILLLLVAPA YSFNCLGMGN RDFIEGASGA 250 260 270 280 290 300 TWVDLVLEGD SCLTIMANDK PTLDVRMINI EAVQLAEVRS YCYHASVTDI STVARCPTTG 310 320 330 340 350 360 EAHNEKRADS SYVCKQGFTD RGWGNGCGLF GKGSIDTCAK FSCTSKAIGR TIQPENIKYE 370 380 390 400 410 420 VGIFVHGTTT SENHGNYSAQ VGASQAAKFT VTPNAPSITL KLGDYGEVTL DCEPRSGLNT 430 440 450 460 470 480 EAFYVMTVGS KSFLVHREWF HDLALPWTPP SSTAWRNREL LMEFEEAHAT KQSVVALGSQ 490 500 510 520 530 540 EGGLHQALAG AIVVEYSSSV KLTSGHLKCR LKMDKLALKG TTYGMCTEKF SFAKNPADTG 550 560 570 580 590 600 HGTVVIELSY SGSDGPCKIP IVSVASLNDM TPVGRLVTVN PFVATSSANS KVLVEMEPPF 610 620 630 640 650 660 GDSYIVVGRG DKQINHHWHK AGSTLGKAFS TTLKGAQRLA ALGDTAWDFG SIGGVFNSIG 670 680 690 700 710 720 KAVHQVFGGA FRTLFGGMSW ITQGLMGALL LWMGVNARDR SIALAFLATG GVLVFLATNV 730 740 750 760 770 780 HADTGCAIDI TRKEMRCGSG IFVHNDVEAW VDRYKYLPET PRSLAKIVHK AHKEGVCGVR 790 800 810 820 830 840 SVTRLEHQMW EAVRDELNVL LKENAVDLSV VVNKPVGRYR SAPKRLSMTQ EKFEMGWKAW 850 860 870 880 890 900 GKSILFAPEL ANSTFVVDGP ETKECPDEHR AWNSIEIEDF GFGITSTRVW LKIREESTDE 910 920 930 940 950 960 CDGAIIGTAV KGHVAVHSDL SYWIESRYND TWKLERAVFG EVKSCTWPET HTLWGDGVEE 970 980 990 1000 1010 1020 SELIIPHTIA GPKSKHNRRE GYKTQNQGPW DENGIVLDFD YCPGTKVTIT EDCGKRGPSV 1030 1040 1050 1060 1070 1080 RTTTDSGKLI TDWCCRSCSL PPLRFRTENG CWYGMEIRPV RHDETTLVRS QVDAFNGEMV 1090 1100 1110 1120 1130 1140 DPFQLGLLVM FLATQEVLRK RWTARLTIPA VLGALLVLML GGITYTDLAR YVVLVAAAFA 1150 1160 1170 1180 1190 1200 EANSGGDVLH LALIAVFKIQ PAFLVMNMLS TRWTNQENVV LVLGAAFFHL ASVDLQIGVH 1210 1220 1230 1240 1250 1260 GILNAAAIAW MIVRAITFPT TSSVTMPVLA LLTPGMRALY LDTYRIILLV IGICSLLQER 1270 1280 1290 1300 1310 1320 KKTMAKKKGA VLLGLALTST GWFSPTTIAA GLMVCNPNKK RGWPATEFLS AVGLMFAIVG 1330 1340 1350 1360 1370 1380 GLAELDIESM SIPFMLAGLM AVSYVVSGKA TDMWLERAAD ISWEMDAAIT GSSRRLDVKL 1390 1400 1410 1420 1430 1440 DDDGDFHLID DPGVPWKVWV LRMSCIGLAA LTPWAIVPAA FGYWLTLKTT KRGGVFWDTP

References

[1]	"Partial nucleotide sequence of the Japanese encephalitis virus genome." McAda P.C., Mason P.W., Schmaljohn C.S., Dalrymple J.M., Mason T.L., Fournier M.J. Virology 158:348-360(1987) [PubMed: 3035787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16574 Genomic RNA. Translation: AAA46251.1.
PIR	GNWVJF. A27844.
3D structure databases
HSSP	HSSP built from PDB template 1OKE based on UniProtKB Q88653.
SMR	P14403. Positions 223-621.
ModBase	Search...
Family and domain databases
InterPro	IPR000069. Env_glycoprot_M_flavivir. IPR013756. Flav_glyE_cen_2. IPR011999. Flav_glyE_cen_dm. IPR013754. Flav_glyE_dim. IPR001122. Flavi_capsidC. IPR001157. Flavi_NS1. IPR000752. Flavi_NS2A. IPR000487. Flavi_NS2B. IPR002535. Flavi_propep. IPR000336. Flv_glyE_Ig-like. [Graphical view]
Gene3D	G3DSA:3.30.67.10. Flav_glyE_cen_2. 1 hit. G3DSA:2.60.98.10. Flav_glyE_dim. 1 hit. G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.
Pfam	PF01003. Flavi_capsid. 1 hit. PF02832. Flavi_glycop_C. 1 hit. PF00869. Flavi_glycoprot. 1 hit. PF01004. Flavi_M. 1 hit. PF00948. Flavi_NS1. 1 hit. PF01005. Flavi_NS2A. 1 hit. PF01002. Flavi_NS2B. 1 hit. PF01570. Flavi_propep. 1 hit. [Graphical view]
ProDom	PD001496. Flavi_NS1. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

POLG_JAEVN

Accession

Primary (citable) accession number: P14403
Secondary accession number(s): P08769

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)