ID   T2TA_THEAQ              Reviewed;         263 AA.
AC   P14386;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 2.
DT   28-JUN-2023, entry version 77.
DE   RecName: Full=Type II restriction enzyme TaqI {ECO:0000303|PubMed:12654995};
DE            Short=R.TaqI;
DE            EC=3.1.21.4;
DE   AltName: Full=Endonuclease TaqI;
DE   AltName: Full=Type-2 restriction enzyme TaqI;
GN   Name=taqIR;
OS   Thermus aquaticus.
OC   Bacteria; Deinococcota; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=271;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-27, PARTIALLY
RP   BLOCKED N-TERMINUS, AND FUNCTION.
RC   STRAIN=ATCC 25104 / DSM 625 / JCM 10724 / NBRC 103206 / NCIMB 11243 /
RC   YT-1;
RX   PubMed=2827113; DOI=10.1093/nar/15.23.9781;
RA   Slatko B.E., Benner J.S., Moran L.S., Jager-Quinton T., Simcox T.G.,
RA   van Cott E.M., Wilson G.G.;
RT   "Cloning, sequencing and expression of the Taq I restriction-modification
RT   system.";
RL   Nucleic Acids Res. 15:9781-9796(1987).
RN   [2]
RP   SEQUENCE REVISION.
RC   STRAIN=ATCC 25104 / DSM 625 / JCM 10724 / NBRC 103206 / NCIMB 11243 /
RC   YT-1;
RX   PubMed=1551602; DOI=10.1016/0378-1119(92)90307-b;
RA   Barany F., Slatko B., Danzitz M., Cowburn D., Schildkraut I., Wilson G.G.;
RT   "The corrected nucleotide sequences of the TaqI restriction and
RT   modification enzymes reveal a thirteen-codon overlap.";
RL   Gene 112:91-95(1992).
RN   [3]
RP   NOMENCLATURE, AND SUBTYPE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC       stranded sequence 5'-TCGA-3' and cleaves after T-1.
CC       {ECO:0000269|PubMed:2827113, ECO:0000303|PubMed:12654995}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC         stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC   -!- PTM: Only 15% of purified enzyme (upon expression in E.coli) can be
CC       sequenced, suggesting the remainder has a blocked N-terminus.
CC       {ECO:0000269|PubMed:2827113}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA68552.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; M74796; AAA27505.1; -; Genomic_DNA.
DR   EMBL; Y00499; CAA68552.1; ALT_FRAME; Genomic_DNA.
DR   PIR; JN0258; JN0258.
DR   AlphaFoldDB; P14386; -.
DR   PRO; PR:P14386; -.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-EC.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   InterPro; IPR019073; Restrct_endonuc_II_TaqI.
DR   Pfam; PF09573; RE_TaqI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Endonuclease; Hydrolase; Nuclease;
KW   Restriction system.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:2827113"
FT   CHAIN           2..263
FT                   /note="Type II restriction enzyme TaqI"
FT                   /id="PRO_0000077369"
SQ   SEQUENCE   263 AA;  30993 MW;  0CE846093E2DC327 CRC64;
     MASTQAQKAL ETFERFLASL DLESYQQKYR PIKTVEQDLP RELNPLPDLY EHYWKALEDN
     PSFLGFEEFF DHWWEKRLRP LDEFIRKYFW GCSYAFVRLG LEARLYRTAV SIWTQFHFCY
     RWNASCELPL EAAPELDAQG IDALIHTSGS STGIQIKKET YRSEAKSENR FLRKQRGTAL
     IEIPYTLQTP EELEEKAKRA RVNGETYRLW AKVAHHLDRL ENGFVIFRES YVKSIELFLQ
     KNAPTLSGLI RWDRVAQEAL TAP
//