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P14386

- T2TA_THEAQ

UniProt

P14386 - T2TA_THEAQ

Protein

Type-2 restriction enzyme TaqI

Gene

taqIR

Organism
Thermus aquaticus
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 62 (16 Apr 2014)
      Sequence version 2 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence TCGA and cleaves after T-1.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Protein family/group databases

    REBASEi1801. TaqI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme TaqI (EC:3.1.21.4)
    Short name:
    R.TaqI
    Alternative name(s):
    Endonuclease TaqI
    Type II restriction enzyme TaqI
    Gene namesi
    Name:taqIR
    OrganismiThermus aquaticus
    Taxonomic identifieri271 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 263263Type-2 restriction enzyme TaqIPRO_0000077369Add
    BLAST

    Family & Domainsi

    Family and domain databases

    InterProiIPR019073. Restrct_endonuc_II_TaqI.
    [Graphical view]
    PfamiPF09573. RE_TaqI. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14386-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTQAQKAL ETFERFLASL DLESYQQKYR PIKTVEQDLP RELNPLPDLY    50
    EHYWKALEDN PSFLGFEEFF DHWWEKRLRP LDEFIRKYFW GCSYAFVRLG 100
    LEARLYRTAV SIWTQFHFCY RWNASCELPL EAAPELDAQG IDALIHTSGS 150
    STGIQIKKET YRSEAKSENR FLRKQRGTAL IEIPYTLQTP EELEEKAKRA 200
    RVNGETYRLW AKVAHHLDRL ENGFVIFRES YVKSIELFLQ KNAPTLSGLI 250
    RWDRVAQEAL TAP 263
    Length:263
    Mass (Da):30,993
    Last modified:April 1, 1993 - v2
    Checksum:i0CE846093E2DC327
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74796 Genomic DNA. Translation: AAA27505.1.
    Y00499 Genomic DNA. Translation: CAA68552.1. Sequence problems.
    PIRiJN0258.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M74796 Genomic DNA. Translation: AAA27505.1 .
    Y00499 Genomic DNA. Translation: CAA68552.1 . Sequence problems.
    PIRi JN0258.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1801. TaqI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR019073. Restrct_endonuc_II_TaqI.
    [Graphical view ]
    Pfami PF09573. RE_TaqI. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequencing and expression of the Taq I restriction-modification system."
      Slatko B.E., Benner J.S., Moran L.S., Jager-Quinton T., Simcox T.G., van Cott E.M., Wilson G.G.
      Nucleic Acids Res. 15:9781-9796(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: YT1.
    2. "The corrected nucleotide sequences of the TaqI restriction and modification enzymes reveal a thirteen-codon overlap."
      Barany F., Slatko B., Danzitz M., Cowburn D., Schildkraut I., Wilson G.G.
      Gene 112:91-95(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
      Strain: YT1.

    Entry informationi

    Entry nameiT2TA_THEAQ
    AccessioniPrimary (citable) accession number: P14386
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: April 1, 1993
    Last modified: April 16, 2014
    This is version 62 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3