P14386 (T2TA_THEAQ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme TaqI
Short name=R.TaqI
EC=3.1.21.4

Alternative name(s):
Endonuclease TaqI
Type II restriction enzyme TaqI

Gene names

Name:

taqIR

Organism

Thermus aquaticus

Taxonomic identifier

271 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Thermales › Thermaceae › Thermus

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence TCGA and cleaves after T-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Type-2 restriction enzyme TaqI		PRO_0000077369

Sequences

	Sequence		Length	Mass (Da)
	P14386 [UniParc]. Last modified April 1, 1993. Version 2. Checksum: 0CE846093E2DC327	FASTA	263	30,993
	10 20 30 40 50 60 MASTQAQKAL ETFERFLASL DLESYQQKYR PIKTVEQDLP RELNPLPDLY EHYWKALEDN 70 80 90 100 110 120 PSFLGFEEFF DHWWEKRLRP LDEFIRKYFW GCSYAFVRLG LEARLYRTAV SIWTQFHFCY 130 140 150 160 170 180 RWNASCELPL EAAPELDAQG IDALIHTSGS STGIQIKKET YRSEAKSENR FLRKQRGTAL 190 200 210 220 230 240 IEIPYTLQTP EELEEKAKRA RVNGETYRLW AKVAHHLDRL ENGFVIFRES YVKSIELFLQ 250 260 KNAPTLSGLI RWDRVAQEAL TAP

References

[1]	"Cloning, sequencing and expression of the Taq I restriction-modification system." Slatko B.E., Benner J.S., Moran L.S., Jager-Quinton T., Simcox T.G., van Cott E.M., Wilson G.G. Nucleic Acids Res. 15:9781-9796(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YT1.
[2]	"The corrected nucleotide sequences of the TaqI restriction and modification enzymes reveal a thirteen-codon overlap." Barany F., Slatko B., Danzitz M., Cowburn D., Schildkraut I., Wilson G.G. Gene 112:91-95(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION. Strain: YT1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M74796 Genomic DNA. Translation: AAA27505.1. Y00499 Genomic DNA. Translation: CAA68552.1. Sequence problems.
PIR	JN0258.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1801. TaqI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR019073. Restrct_endonuc_II_TaqI. [Graphical view]
Pfam	PF09573. RE_TaqI. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T2TA_THEAQ

Accession

Primary (citable) accession number: P14386

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program