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P14386 (T2TA_THEAQ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme TaqI

Short name=R.TaqI
EC=3.1.21.4
Alternative name(s):
Endonuclease TaqI
Type II restriction enzyme TaqI
Gene names
Name:taqIR
OrganismThermus aquaticus
Taxonomic identifier271 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence TCGA and cleaves after T-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: InterPro

Type II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Type-2 restriction enzyme TaqI
PRO_0000077369

Sequences

Sequence LengthMass (Da)Tools
P14386 [UniParc].

Last modified April 1, 1993. Version 2.
Checksum: 0CE846093E2DC327

FASTA26330,993
        10         20         30         40         50         60 
MASTQAQKAL ETFERFLASL DLESYQQKYR PIKTVEQDLP RELNPLPDLY EHYWKALEDN 

        70         80         90        100        110        120 
PSFLGFEEFF DHWWEKRLRP LDEFIRKYFW GCSYAFVRLG LEARLYRTAV SIWTQFHFCY 

       130        140        150        160        170        180 
RWNASCELPL EAAPELDAQG IDALIHTSGS STGIQIKKET YRSEAKSENR FLRKQRGTAL 

       190        200        210        220        230        240 
IEIPYTLQTP EELEEKAKRA RVNGETYRLW AKVAHHLDRL ENGFVIFRES YVKSIELFLQ 

       250        260 
KNAPTLSGLI RWDRVAQEAL TAP 

« Hide

References

[1]"Cloning, sequencing and expression of the Taq I restriction-modification system."
Slatko B.E., Benner J.S., Moran L.S., Jager-Quinton T., Simcox T.G., van Cott E.M., Wilson G.G.
Nucleic Acids Res. 15:9781-9796(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YT1.
[2]"The corrected nucleotide sequences of the TaqI restriction and modification enzymes reveal a thirteen-codon overlap."
Barany F., Slatko B., Danzitz M., Cowburn D., Schildkraut I., Wilson G.G.
Gene 112:91-95(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
Strain: YT1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M74796 Genomic DNA. Translation: AAA27505.1.
Y00499 Genomic DNA. Translation: CAA68552.1. Sequence problems.
PIRJN0258.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1801. TaqI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR019073. Restrct_endonuc_II_TaqI.
[Graphical view]
PfamPF09573. RE_TaqI. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameT2TA_THEAQ
AccessionPrimary (citable) accession number: P14386
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries