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Protein

Type-2 restriction enzyme TaqI

Gene

taqIR

Organism
Thermus aquaticus
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence TCGA and cleaves after T-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi1801. TaqI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme TaqI (EC:3.1.21.4)
Short name:
R.TaqI
Alternative name(s):
Endonuclease TaqI
Type II restriction enzyme TaqI
Gene namesi
Name:taqIR
OrganismiThermus aquaticus
Taxonomic identifieri271 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Type-2 restriction enzyme TaqIPRO_0000077369Add
BLAST

Family & Domainsi

Family and domain databases

InterProiIPR019073. Restrct_endonuc_II_TaqI.
[Graphical view]
PfamiPF09573. RE_TaqI. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14386-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTQAQKAL ETFERFLASL DLESYQQKYR PIKTVEQDLP RELNPLPDLY
60 70 80 90 100
EHYWKALEDN PSFLGFEEFF DHWWEKRLRP LDEFIRKYFW GCSYAFVRLG
110 120 130 140 150
LEARLYRTAV SIWTQFHFCY RWNASCELPL EAAPELDAQG IDALIHTSGS
160 170 180 190 200
STGIQIKKET YRSEAKSENR FLRKQRGTAL IEIPYTLQTP EELEEKAKRA
210 220 230 240 250
RVNGETYRLW AKVAHHLDRL ENGFVIFRES YVKSIELFLQ KNAPTLSGLI
260
RWDRVAQEAL TAP
Length:263
Mass (Da):30,993
Last modified:April 1, 1993 - v2
Checksum:i0CE846093E2DC327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74796 Genomic DNA. Translation: AAA27505.1.
Y00499 Genomic DNA. Translation: CAA68552.1. Sequence problems.
PIRiJN0258.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74796 Genomic DNA. Translation: AAA27505.1.
Y00499 Genomic DNA. Translation: CAA68552.1. Sequence problems.
PIRiJN0258.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1801. TaqI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR019073. Restrct_endonuc_II_TaqI.
[Graphical view]
PfamiPF09573. RE_TaqI. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequencing and expression of the Taq I restriction-modification system."
    Slatko B.E., Benner J.S., Moran L.S., Jager-Quinton T., Simcox T.G., van Cott E.M., Wilson G.G.
    Nucleic Acids Res. 15:9781-9796(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YT1.
  2. "The corrected nucleotide sequences of the TaqI restriction and modification enzymes reveal a thirteen-codon overlap."
    Barany F., Slatko B., Danzitz M., Cowburn D., Schildkraut I., Wilson G.G.
    Gene 112:91-95(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
    Strain: YT1.

Entry informationi

Entry nameiT2TA_THEAQ
AccessioniPrimary (citable) accession number: P14386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.