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Protein

Sensor protein ZraS

Gene

zraS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ZraS/ZraR. May function as a membrane-associated protein kinase that phosphorylates ZraR in response to high concentrations of zinc or lead in the medium.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to lead ion Source: EcoCyc
  • cellular response to zinc ion Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:HYDH-MONOMER.
ECOL316407:JW3967-MONOMER.
BRENDAi2.7.13.3. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein ZraS (EC:2.7.13.3)
Gene namesi
Name:zraS
Synonyms:hydH
Ordered Locus Names:b4003, JW3967
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10008. zraS.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749091 – 465Sensor protein ZraSAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei254Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP14377.
PRIDEiP14377.

Interactioni

Protein-protein interaction databases

BioGridi4263453. 7 interactors.
DIPiDIP-9981N.
IntActiP14377. 1 interactor.
STRINGi511145.b4003.

Structurei

3D structure databases

ProteinModelPortaliP14377.
SMRiP14377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 458Histidine kinasePROSITE-ProRule annotationAdd BLAST208

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000126797.
InParanoidiP14377.
KOiK07709.
OMAiQAIFTPY.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFMQRSKDS LAKWLSAILP VVIVGLVGLF AVTVIRDYGR ASEADRQALL
60 70 80 90 100
EKGNVLIRAL ESGSRVGMGM RMHHVQQQAL LEEMAGQPGV LWFAVTDAQG
110 120 130 140 150
IIILHSDPDK VGRALYSPDE MQKLKPEENS RWRLLGKTET TPALEVYRLF
160 170 180 190 200
QPMSAPWRHG MHNMPRCNGK AVPQVDAQQA IFIAVDASDL VATQSGEKRN
210 220 230 240 250
TLIILFALAT VLLASVLSFF WYRRYLRSRQ LLQDEMKRKE KLVALGHLAA
260 270 280 290 300
GVAHEIRNPL SSIKGLAKYF AERAPAGGEA HQLAQVMAKE ADRLNRVVSE
310 320 330 340 350
LLELVKPTHL ALQAVDLNTL INHSLQLVSQ DANSREIQLR FTANDTLPEI
360 370 380 390 400
QADPDRLTQV LLNLYLNAIQ AIGQHGVISV TASESGAGVK ISVTDSGKGI
410 420 430 440 450
AADQLDAIFT PYFTTKAEGT GLGLAVVHNI VEQHGGTIQV ASQEGKGSTF
460
TLWLPVNITR KDPQG
Length:465
Mass (Da):51,032
Last modified:October 1, 1993 - v2
Checksum:i0472AC3494E95EE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361L → V in AAA24003 (PubMed:2666400).Curated1
Sequence conflicti383 – 387SESGA → TKAG in AAA24003 (PubMed:2666400).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43101.1.
U00096 Genomic DNA. Translation: AAC76977.1.
AP009048 Genomic DNA. Translation: BAE77316.1.
M28369 Genomic DNA. Translation: AAA24003.1.
PIRiF65207.
RefSeqiNP_418431.1. NC_000913.3.
WP_001211892.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76977; AAC76977; b4003.
BAE77316; BAE77316; BAE77316.
GeneIDi948506.
KEGGiecj:JW3967.
eco:b4003.
PATRICi32123531. VBIEscCol129921_4118.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43101.1.
U00096 Genomic DNA. Translation: AAC76977.1.
AP009048 Genomic DNA. Translation: BAE77316.1.
M28369 Genomic DNA. Translation: AAA24003.1.
PIRiF65207.
RefSeqiNP_418431.1. NC_000913.3.
WP_001211892.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP14377.
SMRiP14377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263453. 7 interactors.
DIPiDIP-9981N.
IntActiP14377. 1 interactor.
STRINGi511145.b4003.

Proteomic databases

PaxDbiP14377.
PRIDEiP14377.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76977; AAC76977; b4003.
BAE77316; BAE77316; BAE77316.
GeneIDi948506.
KEGGiecj:JW3967.
eco:b4003.
PATRICi32123531. VBIEscCol129921_4118.

Organism-specific databases

EchoBASEiEB0008.
EcoGeneiEG10008. zraS.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000126797.
InParanoidiP14377.
KOiK07709.
OMAiQAIFTPY.

Enzyme and pathway databases

BioCyciEcoCyc:HYDH-MONOMER.
ECOL316407:JW3967-MONOMER.
BRENDAi2.7.13.3. 2026.

Miscellaneous databases

PROiP14377.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZRAS_ECOLI
AccessioniPrimary (citable) accession number: P14377
Secondary accession number(s): Q2M8U0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1993
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in the regulation of the labile hydrogenase activity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.