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Protein

Sensor protein ZraS

Gene

zraS

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ZraS/ZraR. May function as a membrane-associated protein kinase that phosphorylates ZraR in response to high concentrations of zinc or lead in the medium.1 Publication

Caution

Was originally thought to be involved in the regulation of the labile hydrogenase activity.1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • phosphorelay sensor kinase activity Source: EcoCyc

GO - Biological processi

  • cellular response to lead ion Source: EcoCyc
  • cellular response to zinc ion Source: EcoCyc
  • phosphorelay signal transduction system Source: EcoCyc
  • protein autophosphorylation Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:HYDH-MONOMER
BRENDAi2.7.13.3 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein ZraS (EC:2.7.13.3)
Gene namesi
Name:zraS
Synonyms:hydH
Ordered Locus Names:b4003, JW3967
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10008 zraS

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalSequence analysisAdd BLAST21
Transmembranei202 – 222HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000749091 – 465Sensor protein ZraSAdd BLAST465

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei254Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP14377
PRIDEiP14377

Interactioni

Protein-protein interaction databases

BioGridi4263453, 21 interactors
DIPiDIP-9981N
IntActiP14377, 1 interactor
STRINGi316385.ECDH10B_4192

Structurei

3D structure databases

ProteinModelPortaliP14377
SMRiP14377
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 458Histidine kinasePROSITE-ProRule annotationAdd BLAST208

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU Bacteria
ENOG410XNMH LUCA
HOGENOMiHOG000126797
InParanoidiP14377
KOiK07709
OMAiQAIFTPY

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
Gene3Di3.30.565.10, 1 hit
InterProiView protein in InterPro
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR029151 Sensor-like_sf
IPR004358 Sig_transdc_His_kin-like_C
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SUPFAMiSSF103190 SSF103190, 1 hit
SSF47384 SSF47384, 1 hit
SSF55874 SSF55874, 1 hit
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit

Sequencei

Sequence statusi: Complete.

P14377-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFMQRSKDS LAKWLSAILP VVIVGLVGLF AVTVIRDYGR ASEADRQALL
60 70 80 90 100
EKGNVLIRAL ESGSRVGMGM RMHHVQQQAL LEEMAGQPGV LWFAVTDAQG
110 120 130 140 150
IIILHSDPDK VGRALYSPDE MQKLKPEENS RWRLLGKTET TPALEVYRLF
160 170 180 190 200
QPMSAPWRHG MHNMPRCNGK AVPQVDAQQA IFIAVDASDL VATQSGEKRN
210 220 230 240 250
TLIILFALAT VLLASVLSFF WYRRYLRSRQ LLQDEMKRKE KLVALGHLAA
260 270 280 290 300
GVAHEIRNPL SSIKGLAKYF AERAPAGGEA HQLAQVMAKE ADRLNRVVSE
310 320 330 340 350
LLELVKPTHL ALQAVDLNTL INHSLQLVSQ DANSREIQLR FTANDTLPEI
360 370 380 390 400
QADPDRLTQV LLNLYLNAIQ AIGQHGVISV TASESGAGVK ISVTDSGKGI
410 420 430 440 450
AADQLDAIFT PYFTTKAEGT GLGLAVVHNI VEQHGGTIQV ASQEGKGSTF
460
TLWLPVNITR KDPQG
Length:465
Mass (Da):51,032
Last modified:October 1, 1993 - v2
Checksum:i0472AC3494E95EE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti361L → V in AAA24003 (PubMed:2666400).Curated1
Sequence conflicti383 – 387SESGA → TKAG in AAA24003 (PubMed:2666400).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA Translation: AAC43101.1
U00096 Genomic DNA Translation: AAC76977.1
AP009048 Genomic DNA Translation: BAE77316.1
M28369 Genomic DNA Translation: AAA24003.1
PIRiF65207
RefSeqiNP_418431.1, NC_000913.3
WP_001211892.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76977; AAC76977; b4003
BAE77316; BAE77316; BAE77316
GeneIDi948506
KEGGiecj:JW3967
eco:b4003
PATRICifig|1411691.4.peg.2707

Similar proteinsi

Entry informationi

Entry nameiZRAS_ECOLI
AccessioniPrimary (citable) accession number: P14377
Secondary accession number(s): Q2M8U0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1993
Last modified: March 28, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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