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Protein

Transcriptional regulatory protein ZraR

Gene

zraR

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system ZraS/ZraR. When activated by ZraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi169 – 1768ATPPROSITE-ProRule annotation
Nucleotide bindingi232 – 24110ATPPROSITE-ProRule annotation
DNA bindingi421 – 44020H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: EcoliWiki
  • phosphorelay response regulator activity Source: EcoCyc
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • phosphorelay signal transduction system Source: EcoCyc
  • positive regulation of transcription, DNA-templated Source: EcoCyc
  • transcription, DNA-templated Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:HYDG-MONOMER.
ECOL316407:JW3968-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcriptional regulatory protein ZraR
Gene namesi
Name:zraR
Synonyms:hydG
Ordered Locus Names:b4004, JW3968
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10482. zraR.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441Transcriptional regulatory protein ZraRPRO_0000081279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 5614-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Phosphorylated by ZraS.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP14375.
PRIDEiP14375.

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi4262463. 6 interactions.
DIPiDIP-9980N.
STRINGi511145.b4004.

Structurei

3D structure databases

ProteinModelPortaliP14375.
SMRiP14375. Positions 6-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 121115Response regulatoryPROSITE-ProRule annotationAdd
BLAST
Domaini141 – 370230Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 response regulatory domain.PROSITE-ProRule annotation
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058487.
InParanoidiP14375.
KOiK07713.
OMAiVATDSRW.
OrthoDBiEOG6WHNMG.
PhylomeDBiP14375.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14375-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHDNIDILV VDDDISHCTI LQALLRGWGY NVALANSGRQ ALEQVREQVF
60 70 80 90 100
DLVLCDVRMA EMDGIATLKE IKALNPAIPV LIMTAYSSVE TAVEALKTGA
110 120 130 140 150
LDYLIKPLDF DNLQATLEKA LAHTHSIDAE TPAVTASQFG MVGKSPAMQH
160 170 180 190 200
LLSEIALVAP SEATVLIHGD SGTGKELVAR AIHASSARSE KPLVTLNCAA
210 220 230 240 250
LNESLLESEL FGHEKGAFTG ADKRREGRFV EADGGTLFLD EIGDISPMMQ
260 270 280 290 300
VRLLRAIQER EVQRVGSNQI ISVDVRLIAA THRDLAAEVN AGRFRQDLYY
310 320 330 340 350
RLNVVAIEVP SLRQRREDIP LLAGHFLQRF AERNRKAVKG FTPQAMDLLI
360 370 380 390 400
HYDWPGNIRE LENAVERAVV LLTGEYISER ELPLAIASTP IPLGQSQDIQ
410 420 430 440
PLVEVEKEVI LAALEKTGGN KTEAARQLGI TRKTLLAKLS R
Length:441
Mass (Da):48,395
Last modified:October 1, 1993 - v2
Checksum:iA7BAB1372F374DDD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti117 – 1226LEKALA → WKKRS in AAA24004 (PubMed:2666400).Curated
Sequence conflicti161 – 1611S → C in AAA24004 (PubMed:2666400).Curated
Sequence conflicti172 – 1743GTG → AR in AAA24004 (PubMed:2666400).Curated
Sequence conflicti181 – 1822AI → GL in AAA24004 (PubMed:2666400).Curated
Sequence conflicti228 – 2281R → P in AAA24004 (PubMed:2666400).Curated
Sequence conflicti237 – 2382LF → C in AAA24004 (PubMed:2666400).Curated
Sequence conflicti336 – 34611KAVKGFTPQAM → RGKRFYAPGL in AAA24004 (PubMed:2666400).CuratedAdd
BLAST
Sequence conflicti385 – 3851A → G in AAA24004 (PubMed:2666400).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28369 Genomic DNA. Translation: AAA24004.1.
U00006 Genomic DNA. Translation: AAC43102.1.
U00096 Genomic DNA. Translation: AAC76978.1.
AP009048 Genomic DNA. Translation: BAE77315.1.
PIRiG65207. B33862.
RefSeqiNP_418432.1. NC_000913.3.
WP_000148503.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76978; AAC76978; b4004.
BAE77315; BAE77315; BAE77315.
GeneIDi948505.
KEGGiecj:JW3968.
eco:b4004.
PATRICi32123533. VBIEscCol129921_4119.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M28369 Genomic DNA. Translation: AAA24004.1.
U00006 Genomic DNA. Translation: AAC43102.1.
U00096 Genomic DNA. Translation: AAC76978.1.
AP009048 Genomic DNA. Translation: BAE77315.1.
PIRiG65207. B33862.
RefSeqiNP_418432.1. NC_000913.3.
WP_000148503.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP14375.
SMRiP14375. Positions 6-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262463. 6 interactions.
DIPiDIP-9980N.
STRINGi511145.b4004.

Proteomic databases

PaxDbiP14375.
PRIDEiP14375.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76978; AAC76978; b4004.
BAE77315; BAE77315; BAE77315.
GeneIDi948505.
KEGGiecj:JW3968.
eco:b4004.
PATRICi32123533. VBIEscCol129921_4119.

Organism-specific databases

EchoBASEiEB0477.
EcoGeneiEG10482. zraR.

Phylogenomic databases

eggNOGiENOG4105C1W. Bacteria.
COG2204. LUCA.
HOGENOMiHOG000058487.
InParanoidiP14375.
KOiK07713.
OMAiVATDSRW.
OrthoDBiEOG6WHNMG.
PhylomeDBiP14375.

Enzyme and pathway databases

BioCyciEcoCyc:HYDG-MONOMER.
ECOL316407:JW3968-MONOMER.

Miscellaneous databases

PROiP14375.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011006. CheY-like_superfamily.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR027417. P-loop_NTPase.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF02954. HTH_8. 1 hit.
PF00072. Response_reg. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50110. RESPONSE_REGULATORY. 1 hit.
PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12."
    Stoker K., Reijnders W.N.M., Oltmann L.F., Stouthamer A.H.
    J. Bacteriol. 171:4448-4456(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
    Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
    Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "The hydH/G genes from Escherichia coli code for a zinc and lead responsive two-component regulatory system."
    Leonhartsberger S., Huber A., Lottspeich F., Boeck A.
    J. Mol. Biol. 307:93-105(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF N-TERMINUS.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. "Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli."
    Yamamoto K., Hirao K., Oshima T., Aiba H., Utsumi R., Ishihama A.
    J. Biol. Chem. 280:1448-1456(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiZRAR_ECOLI
AccessioniPrimary (citable) accession number: P14375
Secondary accession number(s): Q2M8U1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1993
Last modified: February 17, 2016
This is version 164 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be involved in the regulation of the labile hydrogenase activity.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.