P14359 (SNA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein SNA3

Gene names

Name:	SNA3
Ordered Locus Names:	YJL151C
ORF Names:	J0630

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Vacuole lumen. Cytoplasmic vesicle membrane; Multi-pass membrane protein Potential. Note: Sorted via late endosomes to the vacuolar lumen in a ubiquitin-independent manner. Ref.6 Ref.7
Miscellaneous	Present with 12500 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UPF0057 (PMP3) family.
Caution	It is uncertain whether Met-1 or Met-13 is the initiator.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle Endosome Membrane Vacuole
Domain	Transmembrane Transmembrane helix
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein catabolic process Inferred from mutant phenotype PubMed 19944104. Source: SGD endosome transport via multivesicular body sorting pathway Inferred from mutant phenotype PubMed 22212814. Source: SGD
Cellular_component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell fungal-type vacuole lumen Inferred from direct assay PubMed 12499386PubMed 17182850. Source: SGD integral to membrane Inferred from sequence model Ref.6PubMed 12192589. Source: SGD late endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin protein ligase binding Inferred from direct assay PubMed 17182849PubMed 17332499. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RSP5	P39940	2	EBI-26122,EBI-16219

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 133	133	Protein SNA3		PRO_0000193990
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Potential
	Topological domain	38 – 48	11	Lumenal Potential
	Transmembrane	49 – 69	21	Helical; Potential
	Topological domain	70 – 133	64	Cytoplasmic Potential
Amino acid modifications
	Modified residue	86	1	Phosphothreonine Ref.11
	Cross-link	125		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P14359 [UniParc]. Last modified April 18, 2006. Version 3. Checksum: DBA26AF5B769C02A	FASTA	133	15,199
	10 20 30 40 50 60 MDRDHINDHD HRMSYSINKD DLLLMVLAVF IPPVAVWKRK GMFNRDTLLN LLLFLLLFFP 70 80 90 100 110 120 AIIHACYVVY ETSSERSYDL SRRHATAPAV DRDLEAHPAE ESQAQPPAYD EDDEAGADVP 130 LMDNKQQLSS GRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biosynthesis of inositol in yeast. Primary structure of myo-inositol-1-phosphate synthase (EC 5.5.1.4) and functional analysis of its structural gene, the INO1 locus." Dean-Johnson M., Henry S.A. J. Biol. Chem. 264:1274-1283(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence analysis of a 40.7 kb segment from the left arm of yeast chromosome X reveals 14 known genes and 13 new open reading frames including homologues of genes clustered on the right arm of chromosome XI." Katsoulou C., Tzermia M., Tavernarakis N., Alexandraki D. Yeast 12:787-797(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Sorting of proteins into multivesicular bodies: ubiquitin-dependent and -independent targeting." Reggiori F., Pelham H.R.B. EMBO J. 20:5176-5186(2001) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery." Hitchcock A.L., Auld K., Gygi S.P., Silver P.A. Proc. Natl. Acad. Sci. U.S.A. 100:12735-12740(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-125, MASS SPECTROMETRY.
[10]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-86, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04453 Genomic DNA. Translation: AAA66309.1. Z49427 Genomic DNA. Translation: CAA89447.1. X87371 Genomic DNA. Translation: CAA60804.1. AY692894 Genomic DNA. Translation: AAT92913.1. BK006943 Genomic DNA. Translation: DAA08651.1.
PIR	B30902. A32209.
RefSeq	NP_012384.1. NM_001181584.1.
3D structure databases
ProteinModelPortal	P14359.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1730N.
IntAct	P14359. 49 interactions.
MINT	MINT-409887.
STRING	4932.YJL151C.
Protein family/group databases
TCDB	9.B.12.3.1. (Salt or low Temperature) stress-induced hydrophobic peptide (SHP) family.
Proteomic databases
PaxDb	P14359.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL151C; YJL151C; YJL151C.
GeneID	853290.
KEGG	sce:YJL151C.
Organism-specific databases
CYGD	YJL151c.
SGD	S000003687. SNA3.
Phylogenomic databases
eggNOG	COG0401.
GeneTree	ENSGT00530000069386.
HOGENOM	HOG000154356.
OrthoDB	EOG4PG99V.
Gene expression databases
Genevestigator	P14359.
GermOnline	YJL151C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000612. PMP3. [Graphical view]
PANTHER	PTHR21659. PTHR21659. 1 hit.
Pfam	PF01679. Pmp3. 1 hit. [Graphical view]
PROSITE	PS01309. UPF0057. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973594.

Entry information

Entry name

SNA3_YEAST

Accession

Primary (citable) accession number: P14359
Secondary accession number(s): D6VW35, Q6B236

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	April 18, 2006
Last modified:	April 3, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program