Reviewed, UniProtKB/Swiss-Prot P14325 (SYQ_DICDI)

Names and origin

Protein names

Recommended name:
    Probable glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS
    Vegetative-specific protein H4

Gene names

Name:	glnS
Synonyms:	cinA, H4
ORF Names:	DDB_G0289481

Organism

Dictyostelium discoideum (Slime mold) [Complete proteome]

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).
Developmental stage	This protein is expressed in growing cells and deactivated upon the initiation of development.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence CAA33446.1 differs from that shown. Reason: Frameshift at positions 145, 155 and 284.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Probable glutaminyl-tRNA synthetase		PRO_0000195862
Experimental info
	Sequence conflict	219	1	L → F in CAA33446. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14325-1 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 2249628C92988AD1	FASTA	779	88,426
	10 20 30 40 50 60 MSTKPTINKD ELVTLFSQIG LDSSKAKETT NNATLSSNLQ EIIKEAGAES GCEKSVGLLL 70 80 90 100 110 120 YTLATKYPAN AMKHRATLVD YIANKKSVNS INLQACLDYL RRTANEELNV AEFEQSCGVG 130 140 150 160 170 180 VVITREQVAQ AVSDYINKNK SDLLEKRYQF NIGGILMEIK NSLKWANAKD IKEEVDAAIL 190 200 210 220 230 240 SLLGPKTDAD KAPPAKPVKP TTPTAVATTT AATTTTGDLS PIIPAELKPA KEEIKFPDPS 250 260 270 280 290 300 DNIQNTPKLL ADHLKTTGGK IVTRFPPEPN GYLHIGHAKA MHLNFGYAKK NGGKCYLRFD 310 320 330 340 350 360 DTNPEKENQE YIDSIIDSVK WLGHEPCEIT YSSSQFDTLY EMANELIRRG YAYVCHQTAS 370 380 390 400 410 420 EISEGREKMT DSPYRNRTVE ENLKLFEDMR LGKFEEGKAI LRMKGDMKHP NPCMRDLIAY 430 440 450 460 470 480 RIKYHHHPMS GDKWCIYPSY DYTHCLVDSI ENITHSLCTL EFEIRRLTYN WLIDVLGLYR 490 500 510 520 530 540 PVVWEYARLN LTHTVLSKRK IITLVQNKIV NGWDDPRLST LNAFRRKGYT PEAINLLCDT 550 560 570 580 590 600 IGVTRTNGTT ISYELLELCC RQDLDGKATR AMAVFDPIKV VITNYPEDKS EEINAPNIPS 610 620 630 640 650 660 KPEKGTHKID FSRIVYIERS DFRMEDNKDF FGLAPGKEIL LKYAYNIKCE KVIQDADGKV 670 680 690 700 710 720 TELHVTYDKD NSSKKLKTIH WVSSVAGTEP MKAEVRLYEH LFKDSEIGDD WLNNINPNSL 730 740 750 760 770 RIIPNAFIDK TVLASKEYDR YQFERVGYFV VDKDTTSDKM VFNRTVSLKE NKEKSKSRN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[2]	"Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum." Singleton C.K., Manning S.S., Ken R. Nucleic Acids Res. 17:9679-9692(1989) [PubMed: 2602140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288. Strain: AX3.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFI02000141 Genomic DNA. Translation: EAL62672.1. X15388 Genomic DNA. Translation: CAA33446.1. Frameshift.
PIR	S07563.
RefSeq	XP_636180.1.
3D structure databases
HSSP	HSSP built from PDB template 1GTR based on UniProtKB P00962.
ModBase	Search...
Protein-protein interaction databases
STRING	P14325.
Genome annotation databases
GeneID	3387749.
GenomeReviews	Gene locus glnS in contig CM000154_GR.
KEGG	ddi:DDB_0201644.
Organism-specific databases
dictyBase	DDB_G0289481. glnS.
Phylogenomic databases
OMA	RMPTIAG.
Enzyme and pathway databases
BRENDA	6.1.1.18. 424.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR007639. Gln-tRNA-synth_Ic_RNA-bd_1. IPR007638. Gln-tRNA-synth_Ic_RNA-bd_2. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 2 hits. PTHR10119. Glu_tRNA-synt_1c. 2 hits.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. PF04558. tRNA_synt_1c_R1. 1 hit. PF04557. tRNA_synt_1c_R2. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_DICDI

Accession

Primary (citable) accession number: P14325
Secondary accession number(s): Q54HF7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	December 4, 2007
Last modified:	November 3, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)