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P14325 (SYQ_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Vegetative-specific protein H4
Gene names
Name:glnS
Synonyms:cinA, H4
ORF Names:DDB_G0289481
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length779 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Developmental stage

This protein is expressed in growing cells and deactivated upon the initiation of development.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence CAA33446.1 differs from that shown. Reason: Frameshift at positions 145, 155 and 284.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 779779Probable glutamine--tRNA ligase
PRO_0000195862

Experimental info

Sequence conflict2191L → F in CAA33446. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P14325 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: 2249628C92988AD1

FASTA77988,426
        10         20         30         40         50         60 
MSTKPTINKD ELVTLFSQIG LDSSKAKETT NNATLSSNLQ EIIKEAGAES GCEKSVGLLL 

        70         80         90        100        110        120 
YTLATKYPAN AMKHRATLVD YIANKKSVNS INLQACLDYL RRTANEELNV AEFEQSCGVG 

       130        140        150        160        170        180 
VVITREQVAQ AVSDYINKNK SDLLEKRYQF NIGGILMEIK NSLKWANAKD IKEEVDAAIL 

       190        200        210        220        230        240 
SLLGPKTDAD KAPPAKPVKP TTPTAVATTT AATTTTGDLS PIIPAELKPA KEEIKFPDPS 

       250        260        270        280        290        300 
DNIQNTPKLL ADHLKTTGGK IVTRFPPEPN GYLHIGHAKA MHLNFGYAKK NGGKCYLRFD 

       310        320        330        340        350        360 
DTNPEKENQE YIDSIIDSVK WLGHEPCEIT YSSSQFDTLY EMANELIRRG YAYVCHQTAS 

       370        380        390        400        410        420 
EISEGREKMT DSPYRNRTVE ENLKLFEDMR LGKFEEGKAI LRMKGDMKHP NPCMRDLIAY 

       430        440        450        460        470        480 
RIKYHHHPMS GDKWCIYPSY DYTHCLVDSI ENITHSLCTL EFEIRRLTYN WLIDVLGLYR 

       490        500        510        520        530        540 
PVVWEYARLN LTHTVLSKRK IITLVQNKIV NGWDDPRLST LNAFRRKGYT PEAINLLCDT 

       550        560        570        580        590        600 
IGVTRTNGTT ISYELLELCC RQDLDGKATR AMAVFDPIKV VITNYPEDKS EEINAPNIPS 

       610        620        630        640        650        660 
KPEKGTHKID FSRIVYIERS DFRMEDNKDF FGLAPGKEIL LKYAYNIKCE KVIQDADGKV 

       670        680        690        700        710        720 
TELHVTYDKD NSSKKLKTIH WVSSVAGTEP MKAEVRLYEH LFKDSEIGDD WLNNINPNSL 

       730        740        750        760        770 
RIIPNAFIDK TVLASKEYDR YQFERVGYFV VDKDTTSDKM VFNRTVSLKE NKEKSKSRN 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[2]"Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum."
Singleton C.K., Manning S.S., Ken R.
Nucleic Acids Res. 17:9679-9692(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288.
Strain: AX3.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AAFI02000141 Genomic DNA. Translation: EAL62672.1.
X15388 Genomic DNA. Translation: CAA33446.1. Frameshift.
PIRS07563.
RefSeqXP_636180.1. XM_631088.1.

3D structure databases

ProteinModelPortalP14325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0201644.

Proteomic databases

PRIDEP14325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0201644; DDB0201644; DDB_G0289481.
GeneID8627167.
KEGGddi:DDB_G0289481.

Organism-specific databases

dictyBaseDDB_G0289481. glnS.

Phylogenomic databases

eggNOGCOG0008.
OMAEGHVRDW.
PhylomeDBP14325.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP14325.

Entry information

Entry nameSYQ_DICDI
AccessionPrimary (citable) accession number: P14325
Secondary accession number(s): Q54HF7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 4, 2007
Last modified: July 9, 2014
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries