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P14325

- SYQ_DICDI

UniProt

P14325 - SYQ_DICDI

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Protein

Probable glutamine--tRNA ligase

Gene

glnS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamine-tRNA ligase activity Source: dictyBase

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: dictyBase
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_221378. Mitochondrial tRNA aminoacylation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Vegetative-specific protein H4
Gene namesi
Name:glnS
Synonyms:cinA, H4
ORF Names:DDB_G0289481
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

Organism-specific databases

dictyBaseiDDB_G0289481. glnS.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: dictyBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 779779Probable glutamine--tRNA ligasePRO_0000195862Add
BLAST

Proteomic databases

PRIDEiP14325.

Expressioni

Developmental stagei

This protein is expressed in growing cells and deactivated upon the initiation of development.

Interactioni

Protein-protein interaction databases

STRINGi44689.DDB_0201644.

Structurei

3D structure databases

ProteinModelPortaliP14325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
InParanoidiP14325.
KOiK01886.
OMAiEGHVRDW.
PhylomeDBiP14325.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14325-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTKPTINKD ELVTLFSQIG LDSSKAKETT NNATLSSNLQ EIIKEAGAES
60 70 80 90 100
GCEKSVGLLL YTLATKYPAN AMKHRATLVD YIANKKSVNS INLQACLDYL
110 120 130 140 150
RRTANEELNV AEFEQSCGVG VVITREQVAQ AVSDYINKNK SDLLEKRYQF
160 170 180 190 200
NIGGILMEIK NSLKWANAKD IKEEVDAAIL SLLGPKTDAD KAPPAKPVKP
210 220 230 240 250
TTPTAVATTT AATTTTGDLS PIIPAELKPA KEEIKFPDPS DNIQNTPKLL
260 270 280 290 300
ADHLKTTGGK IVTRFPPEPN GYLHIGHAKA MHLNFGYAKK NGGKCYLRFD
310 320 330 340 350
DTNPEKENQE YIDSIIDSVK WLGHEPCEIT YSSSQFDTLY EMANELIRRG
360 370 380 390 400
YAYVCHQTAS EISEGREKMT DSPYRNRTVE ENLKLFEDMR LGKFEEGKAI
410 420 430 440 450
LRMKGDMKHP NPCMRDLIAY RIKYHHHPMS GDKWCIYPSY DYTHCLVDSI
460 470 480 490 500
ENITHSLCTL EFEIRRLTYN WLIDVLGLYR PVVWEYARLN LTHTVLSKRK
510 520 530 540 550
IITLVQNKIV NGWDDPRLST LNAFRRKGYT PEAINLLCDT IGVTRTNGTT
560 570 580 590 600
ISYELLELCC RQDLDGKATR AMAVFDPIKV VITNYPEDKS EEINAPNIPS
610 620 630 640 650
KPEKGTHKID FSRIVYIERS DFRMEDNKDF FGLAPGKEIL LKYAYNIKCE
660 670 680 690 700
KVIQDADGKV TELHVTYDKD NSSKKLKTIH WVSSVAGTEP MKAEVRLYEH
710 720 730 740 750
LFKDSEIGDD WLNNINPNSL RIIPNAFIDK TVLASKEYDR YQFERVGYFV
760 770
VDKDTTSDKM VFNRTVSLKE NKEKSKSRN
Length:779
Mass (Da):88,426
Last modified:December 4, 2007 - v2
Checksum:i2249628C92988AD1
GO

Sequence cautioni

The sequence CAA33446.1 differs from that shown. Reason: Frameshift at positions 145, 155 and 284.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti219 – 2191L → F in CAA33446. (PubMed:2602140)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000141 Genomic DNA. Translation: EAL62672.1.
X15388 Genomic DNA. Translation: CAA33446.1. Frameshift.
PIRiS07563.
RefSeqiXP_636180.1. XM_631088.1.

Genome annotation databases

EnsemblProtistsiDDB0201644; DDB0201644; DDB_G0289481.
GeneIDi8627167.
KEGGiddi:DDB_G0289481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AAFI02000141 Genomic DNA. Translation: EAL62672.1 .
X15388 Genomic DNA. Translation: CAA33446.1 . Frameshift.
PIRi S07563.
RefSeqi XP_636180.1. XM_631088.1.

3D structure databases

ProteinModelPortali P14325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 44689.DDB_0201644.

Proteomic databases

PRIDEi P14325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi DDB0201644 ; DDB0201644 ; DDB_G0289481 .
GeneIDi 8627167.
KEGGi ddi:DDB_G0289481.

Organism-specific databases

dictyBasei DDB_G0289481. glnS.

Phylogenomic databases

eggNOGi COG0008.
InParanoidi P14325.
KOi K01886.
OMAi EGHVRDW.
PhylomeDBi P14325.

Enzyme and pathway databases

Reactomei REACT_221378. Mitochondrial tRNA aminoacylation.

Miscellaneous databases

PROi P14325.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
PF04558. tRNA_synt_1c_R1. 1 hit.
PF04557. tRNA_synt_1c_R2. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00440. glnS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the social amoeba Dictyostelium discoideum."
    Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
    , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
    Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX4.
  2. "Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum."
    Singleton C.K., Manning S.S., Ken R.
    Nucleic Acids Res. 17:9679-9692(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288.
    Strain: AX3.

Entry informationi

Entry nameiSYQ_DICDI
AccessioniPrimary (citable) accession number: P14325
Secondary accession number(s): Q54HF7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 4, 2007
Last modified: October 29, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3