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P14325

- SYQ_DICDI

UniProt

P14325 - SYQ_DICDI

Protein

Probable glutamine--tRNA ligase

Gene

glnS

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamine-tRNA ligase activity Source: dictyBase

    GO - Biological processi

    1. glutaminyl-tRNA aminoacylation Source: dictyBase

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_221378. Mitochondrial tRNA aminoacylation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable glutamine--tRNA ligase (EC:6.1.1.18)
    Alternative name(s):
    Glutaminyl-tRNA synthetase
    Short name:
    GlnRS
    Vegetative-specific protein H4
    Gene namesi
    Name:glnS
    Synonyms:cinA, H4
    ORF Names:DDB_G0289481
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 5, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0289481. glnS.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 779779Probable glutamine--tRNA ligasePRO_0000195862Add
    BLAST

    Proteomic databases

    PRIDEiP14325.

    Expressioni

    Developmental stagei

    This protein is expressed in growing cells and deactivated upon the initiation of development.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0201644.

    Structurei

    3D structure databases

    ProteinModelPortaliP14325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0008.
    KOiK01886.
    OMAiEGHVRDW.
    PhylomeDBiP14325.

    Family and domain databases

    Gene3Di1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR004514. Gln-tRNA-synth.
    IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
    IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    PF04558. tRNA_synt_1c_R1. 1 hit.
    PF04557. tRNA_synt_1c_R2. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF50715. SSF50715. 1 hit.
    TIGRFAMsiTIGR00440. glnS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14325-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTKPTINKD ELVTLFSQIG LDSSKAKETT NNATLSSNLQ EIIKEAGAES    50
    GCEKSVGLLL YTLATKYPAN AMKHRATLVD YIANKKSVNS INLQACLDYL 100
    RRTANEELNV AEFEQSCGVG VVITREQVAQ AVSDYINKNK SDLLEKRYQF 150
    NIGGILMEIK NSLKWANAKD IKEEVDAAIL SLLGPKTDAD KAPPAKPVKP 200
    TTPTAVATTT AATTTTGDLS PIIPAELKPA KEEIKFPDPS DNIQNTPKLL 250
    ADHLKTTGGK IVTRFPPEPN GYLHIGHAKA MHLNFGYAKK NGGKCYLRFD 300
    DTNPEKENQE YIDSIIDSVK WLGHEPCEIT YSSSQFDTLY EMANELIRRG 350
    YAYVCHQTAS EISEGREKMT DSPYRNRTVE ENLKLFEDMR LGKFEEGKAI 400
    LRMKGDMKHP NPCMRDLIAY RIKYHHHPMS GDKWCIYPSY DYTHCLVDSI 450
    ENITHSLCTL EFEIRRLTYN WLIDVLGLYR PVVWEYARLN LTHTVLSKRK 500
    IITLVQNKIV NGWDDPRLST LNAFRRKGYT PEAINLLCDT IGVTRTNGTT 550
    ISYELLELCC RQDLDGKATR AMAVFDPIKV VITNYPEDKS EEINAPNIPS 600
    KPEKGTHKID FSRIVYIERS DFRMEDNKDF FGLAPGKEIL LKYAYNIKCE 650
    KVIQDADGKV TELHVTYDKD NSSKKLKTIH WVSSVAGTEP MKAEVRLYEH 700
    LFKDSEIGDD WLNNINPNSL RIIPNAFIDK TVLASKEYDR YQFERVGYFV 750
    VDKDTTSDKM VFNRTVSLKE NKEKSKSRN 779
    Length:779
    Mass (Da):88,426
    Last modified:December 4, 2007 - v2
    Checksum:i2249628C92988AD1
    GO

    Sequence cautioni

    The sequence CAA33446.1 differs from that shown. Reason: Frameshift at positions 145, 155 and 284.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti219 – 2191L → F in CAA33446. (PubMed:2602140)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000141 Genomic DNA. Translation: EAL62672.1.
    X15388 Genomic DNA. Translation: CAA33446.1. Frameshift.
    PIRiS07563.
    RefSeqiXP_636180.1. XM_631088.1.

    Genome annotation databases

    EnsemblProtistsiDDB0201644; DDB0201644; DDB_G0289481.
    GeneIDi8627167.
    KEGGiddi:DDB_G0289481.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAFI02000141 Genomic DNA. Translation: EAL62672.1 .
    X15388 Genomic DNA. Translation: CAA33446.1 . Frameshift.
    PIRi S07563.
    RefSeqi XP_636180.1. XM_631088.1.

    3D structure databases

    ProteinModelPortali P14325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0201644.

    Proteomic databases

    PRIDEi P14325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0201644 ; DDB0201644 ; DDB_G0289481 .
    GeneIDi 8627167.
    KEGGi ddi:DDB_G0289481.

    Organism-specific databases

    dictyBasei DDB_G0289481. glnS.

    Phylogenomic databases

    eggNOGi COG0008.
    KOi K01886.
    OMAi EGHVRDW.
    PhylomeDBi P14325.

    Enzyme and pathway databases

    Reactomei REACT_221378. Mitochondrial tRNA aminoacylation.

    Miscellaneous databases

    PROi P14325.

    Family and domain databases

    Gene3Di 1.10.1160.10. 1 hit.
    2.40.240.10. 2 hits.
    3.40.50.620. 2 hits.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR004514. Gln-tRNA-synth.
    IPR007638. Gln-tRNA-synth_Ib_RNA-bd_2.
    IPR007639. Gln-tRNA-synth_Ib_RNA-bd_N.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
    IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
    IPR011035. Ribosomal_L25/Gln-tRNA_synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    PF03950. tRNA-synt_1c_C. 1 hit.
    PF04558. tRNA_synt_1c_R1. 1 hit.
    PF04557. tRNA_synt_1c_R2. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF50715. SSF50715. 1 hit.
    TIGRFAMsi TIGR00440. glnS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    2. "Primary structure and regulation of vegetative specific genes of Dictyostelium discoideum."
      Singleton C.K., Manning S.S., Ken R.
      Nucleic Acids Res. 17:9679-9692(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-288.
      Strain: AX3.

    Entry informationi

    Entry nameiSYQ_DICDI
    AccessioniPrimary (citable) accession number: P14325
    Secondary accession number(s): Q54HF7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 98 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3