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P14306 (CPYI_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxypeptidase Y inhibitor

Short name=CPY inhibitor
Alternative name(s):
CDC25 suppressor 1
I(C)
Ic
Protein DKA1
Protein NSP1
Gene names
Name:TFS1
Synonyms:DKA1, NSP1
Ordered Locus Names:YLR178C
ORF Names:L9470.19
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Specific and potent inhibitor of carboxypeptidase Y. Ref.5

Subunit structure

Monomer.

Subcellular location

Cytoplasm.

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the phosphatidylethanolamine-binding protein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 219219Carboxypeptidase Y inhibitor
PRO_0000204753

Amino acid modifications

Modified residue11N-acetylmethionine Ref.6

Experimental info

Sequence conflict561K → N in CAA44015. Ref.2
Sequence conflict1651F → L in CAA33456. Ref.1
Sequence conflict211 – 2199SNFFYAETK → VQFLLCGNEIGIYIYICICI YFLDFSAFHLTFYYFCFIYV FVTNGQMFVGTNVYVKQNT Ref.1

Secondary structure

.................................... 219
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P14306 [UniParc].

Last modified July 1, 1993. Version 2.
Checksum: 37D4FEC17677A573

FASTA21924,357
        10         20         30         40         50         60 
MNQAIDFAQA SIDSYKKHGI LEDVIHDTSF QPSGILAVEY SSSAPVAMGN TLPTEKARSK 

        70         80         90        100        110        120 
PQFQFTFNKQ MQKSVPQANA YVPQDDDLFT LVMTDPDAPS KTDHKWSEFC HLVECDLKLL 

       130        140        150        160        170        180 
NEATHETSGA TEFFASEFNT KGSNTLIEYM GPAPPKGSGP HRYVFLLYKQ PKGVDSSKFS 

       190        200        210 
KIKDRPNWGY GTPATGVGKW AKENNLQLVA SNFFYAETK 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of NSP1, a locus encoding a component of a CDC25-dependent, nutrient-responsive pathway in Saccharomyces cerevisiae."
Tripp M.L., Bouchard R.A., Pinon R.
Mol. Microbiol. 3:1319-1327(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: X241.
[2]"TFS1: a suppressor of cdc25 mutations in Saccharomyces cerevisiae."
Robinson L.C., Tatchell K.
Mol. Gen. Genet. 230:241-250(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H. expand/collapse author list , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"A high-affinity inhibitor of yeast carboxypeptidase Y is encoded by TFS1 and shows homology to a family of lipid binding proteins."
Bruun A.W., Svendsen I., Sorensen S.O., Kielland-Brandt M.C., Winther J.R.
Biochemistry 37:3351-3357(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae."
Mima J., Suzuki H., Takahashi M., Hayashi R.
J. Biochem. 132:967-973(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, ACETYLATION AT MET-1.
[7]"N-terminal acetyl group is essential for the inhibitory function of carboxypeptidase Y inhibitor (I(C))."
Mima J., Kondo T., Hayashi R.
FEBS Lett. 532:207-210(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15409 Genomic DNA. Translation: CAA33456.1.
X62105 Genomic DNA. Translation: CAA44015.1.
U17246 Genomic DNA. Translation: AAB67471.1.
BK006945 Genomic DNA. Translation: DAA09498.1.
PIRS18843.
RefSeqNP_013279.1. NM_001182065.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1WPXX-ray2.70B1-219[»]
ProteinModelPortalP14306.
SMRP14306. Positions 1-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31449. 28 interactions.
DIPDIP-669N.
IntActP14306. 3 interactions.
MINTMINT-2786931.
STRING4932.YLR178C.

Protein family/group databases

MEROPSI51.001.

Proteomic databases

MaxQBP14306.
PaxDbP14306.
PeptideAtlasP14306.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYLR178C; YLR178C; YLR178C.
GeneID850875.
KEGGsce:YLR178C.

Organism-specific databases

CYGDYLR178c.
SGDS000004168. TFS1.

Phylogenomic databases

eggNOGCOG1881.
GeneTreeENSGT00740000116453.
HOGENOMHOG000237655.
KOK06910.
OMAAPSNTDH.
OrthoDBEOG70612X.

Enzyme and pathway databases

BioCycYEAST:G3O-32303-MONOMER.

Gene expression databases

GenevestigatorP14306.

Family and domain databases

Gene3D3.90.280.10. 1 hit.
InterProIPR001858. Phosphotidylethanolamine-bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view]
PfamPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMSSF49777. SSF49777. 1 hit.
PROSITEPS01220. PBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP14306.
NextBio967218.

Entry information

Entry nameCPYI_YEAST
AccessionPrimary (citable) accession number: P14306
Secondary accession number(s): D6VYI2, P30312
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XII

Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references