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P14306

- CPYI_YEAST

UniProt

P14306 - CPYI_YEAST

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Protein

Carboxypeptidase Y inhibitor

Gene

TFS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor of carboxypeptidase Y.1 Publication

GO - Molecular functioni

  1. lipid binding Source: SGD
  2. peptidase inhibitor activity Source: SGD
  3. phospholipid binding Source: SGD
  4. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  1. regulation of proteolysis Source: SGD
  2. regulation of Ras protein signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32303-MONOMER.

Protein family/group databases

MEROPSiI51.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase Y inhibitor
Short name:
CPY inhibitor
Alternative name(s):
CDC25 suppressor 1
I(C)
Ic
Protein DKA1
Protein NSP1
Gene namesi
Name:TFS1
Synonyms:DKA1, NSP1
Ordered Locus Names:YLR178C
ORF Names:L9470.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR178c.
SGDiS000004168. TFS1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. fungal-type vacuole lumen Source: SGD
  3. fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 219219Carboxypeptidase Y inhibitorPRO_0000204753Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP14306.
PaxDbiP14306.
PeptideAtlasiP14306.

Expressioni

Gene expression databases

GenevestigatoriP14306.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi31449. 29 interactions.
DIPiDIP-669N.
IntActiP14306. 3 interactions.
MINTiMINT-2786931.
STRINGi4932.YLR178C.

Structurei

Secondary structure

1
219
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43
Helixi7 – 1711
Helixi20 – 234
Beta strandi33 – 419
Beta strandi44 – 463
Helixi54 – 574
Beta strandi62 – 687
Beta strandi89 – 9911
Beta strandi108 – 11912
Beta strandi133 – 1397
Beta strandi144 – 1474
Beta strandi161 – 1699
Helixi176 – 1783
Helixi187 – 1893
Beta strandi192 – 1943
Helixi197 – 2026
Turni203 – 2053
Beta strandi207 – 21610

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WPXX-ray2.70B1-219[»]
ProteinModelPortaliP14306.
SMRiP14306. Positions 1-219.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14306.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1881.
GeneTreeiENSGT00740000116453.
HOGENOMiHOG000237655.
InParanoidiP14306.
KOiK06910.
OMAiAPSNTDH.
OrthoDBiEOG70612X.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR001858. Phosphotidylethanolamine-bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14306-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNQAIDFAQA SIDSYKKHGI LEDVIHDTSF QPSGILAVEY SSSAPVAMGN
60 70 80 90 100
TLPTEKARSK PQFQFTFNKQ MQKSVPQANA YVPQDDDLFT LVMTDPDAPS
110 120 130 140 150
KTDHKWSEFC HLVECDLKLL NEATHETSGA TEFFASEFNT KGSNTLIEYM
160 170 180 190 200
GPAPPKGSGP HRYVFLLYKQ PKGVDSSKFS KIKDRPNWGY GTPATGVGKW
210
AKENNLQLVA SNFFYAETK
Length:219
Mass (Da):24,357
Last modified:July 1, 1993 - v2
Checksum:i37D4FEC17677A573
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti56 – 561K → N in CAA44015. (PubMed:1745232)Curated
Sequence conflicti165 – 1651F → L in CAA33456. (PubMed:2693892)Curated
Sequence conflicti211 – 2199SNFFYAETK → VQFLLCGNEIGIYIYICICI YFLDFSAFHLTFYYFCFIYV FVTNGQMFVGTNVYVKQNT(PubMed:2693892)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15409 Genomic DNA. Translation: CAA33456.1.
X62105 Genomic DNA. Translation: CAA44015.1.
U17246 Genomic DNA. Translation: AAB67471.1.
BK006945 Genomic DNA. Translation: DAA09498.1.
PIRiS18843.
RefSeqiNP_013279.1. NM_001182065.1.

Genome annotation databases

EnsemblFungiiYLR178C; YLR178C; YLR178C.
GeneIDi850875.
KEGGisce:YLR178C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15409 Genomic DNA. Translation: CAA33456.1 .
X62105 Genomic DNA. Translation: CAA44015.1 .
U17246 Genomic DNA. Translation: AAB67471.1 .
BK006945 Genomic DNA. Translation: DAA09498.1 .
PIRi S18843.
RefSeqi NP_013279.1. NM_001182065.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1WPX X-ray 2.70 B 1-219 [» ]
ProteinModelPortali P14306.
SMRi P14306. Positions 1-219.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31449. 29 interactions.
DIPi DIP-669N.
IntActi P14306. 3 interactions.
MINTi MINT-2786931.
STRINGi 4932.YLR178C.

Protein family/group databases

MEROPSi I51.001.

Proteomic databases

MaxQBi P14306.
PaxDbi P14306.
PeptideAtlasi P14306.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR178C ; YLR178C ; YLR178C .
GeneIDi 850875.
KEGGi sce:YLR178C.

Organism-specific databases

CYGDi YLR178c.
SGDi S000004168. TFS1.

Phylogenomic databases

eggNOGi COG1881.
GeneTreei ENSGT00740000116453.
HOGENOMi HOG000237655.
InParanoidi P14306.
KOi K06910.
OMAi APSNTDH.
OrthoDBi EOG70612X.

Enzyme and pathway databases

BioCyci YEAST:G3O-32303-MONOMER.

Miscellaneous databases

EvolutionaryTracei P14306.
NextBioi 967218.

Gene expression databases

Genevestigatori P14306.

Family and domain databases

Gene3Di 3.90.280.10. 1 hit.
InterProi IPR001858. Phosphotidylethanolamine-bd_CS.
IPR008914. PtdEtn-bd_prot_PEBP.
[Graphical view ]
Pfami PF01161. PBP. 1 hit.
[Graphical view ]
SUPFAMi SSF49777. SSF49777. 1 hit.
PROSITEi PS01220. PBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of NSP1, a locus encoding a component of a CDC25-dependent, nutrient-responsive pathway in Saccharomyces cerevisiae."
    Tripp M.L., Bouchard R.A., Pinon R.
    Mol. Microbiol. 3:1319-1327(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: X241.
  2. "TFS1: a suppressor of cdc25 mutations in Saccharomyces cerevisiae."
    Robinson L.C., Tatchell K.
    Mol. Gen. Genet. 230:241-250(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "A high-affinity inhibitor of yeast carboxypeptidase Y is encoded by TFS1 and shows homology to a family of lipid binding proteins."
    Bruun A.W., Svendsen I., Sorensen S.O., Kielland-Brandt M.C., Winther J.R.
    Biochemistry 37:3351-3357(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Overexpression and functional characterization of a serine carboxypeptidase inhibitor (I(C)) from Saccharomyces cerevisiae."
    Mima J., Suzuki H., Takahashi M., Hayashi R.
    J. Biochem. 132:967-973(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, ACETYLATION AT MET-1.
  7. "N-terminal acetyl group is essential for the inhibitory function of carboxypeptidase Y inhibitor (I(C))."
    Mima J., Kondo T., Hayashi R.
    FEBS Lett. 532:207-210(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCPYI_YEAST
AccessioniPrimary (citable) accession number: P14306
Secondary accession number(s): D6VYI2, P30312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3