Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Carboxypeptidase Y inhibitor

Gene

TFS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specific and potent inhibitor of carboxypeptidase Y.1 Publication

GO - Molecular functioni

  • lipid binding Source: SGD
  • peptidase inhibitor activity Source: SGD
  • phospholipid binding Source: SGD
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

  • regulation of proteolysis Source: SGD
  • regulation of Ras protein signal transduction Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Enzyme and pathway databases

BioCyciYEAST:G3O-32303-MONOMER.

Protein family/group databases

MEROPSiI51.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Carboxypeptidase Y inhibitor
Short name:
CPY inhibitor
Alternative name(s):
CDC25 suppressor 1
I(C)
Ic
Protein DKA1
Protein NSP1
Gene namesi
Name:TFS1
Synonyms:DKA1, NSP1
Ordered Locus Names:YLR178C
ORF Names:L9470.19
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR178C.
SGDiS000004168. TFS1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • fungal-type vacuole lumen Source: SGD
  • fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002047531 – 219Carboxypeptidase Y inhibitorAdd BLAST219

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP14306.
PRIDEiP14306.
TopDownProteomicsiP14306.

PTM databases

iPTMnetiP14306.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi31449. 30 interactors.
DIPiDIP-669N.
IntActiP14306. 3 interactors.
MINTiMINT-2786931.

Structurei

Secondary structure

1219
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Helixi7 – 17Combined sources11
Helixi20 – 23Combined sources4
Beta strandi33 – 41Combined sources9
Beta strandi44 – 46Combined sources3
Helixi54 – 57Combined sources4
Beta strandi62 – 68Combined sources7
Beta strandi89 – 99Combined sources11
Beta strandi108 – 119Combined sources12
Beta strandi133 – 139Combined sources7
Beta strandi144 – 147Combined sources4
Beta strandi161 – 169Combined sources9
Helixi176 – 178Combined sources3
Helixi187 – 189Combined sources3
Beta strandi192 – 194Combined sources3
Helixi197 – 202Combined sources6
Turni203 – 205Combined sources3
Beta strandi207 – 216Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WPXX-ray2.70B1-219[»]
ProteinModelPortaliP14306.
SMRiP14306.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14306.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
InParanoidiP14306.
KOiK06910.
OMAiSGLHRYT.
OrthoDBiEOG092C48WA.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQAIDFAQA SIDSYKKHGI LEDVIHDTSF QPSGILAVEY SSSAPVAMGN
60 70 80 90 100
TLPTEKARSK PQFQFTFNKQ MQKSVPQANA YVPQDDDLFT LVMTDPDAPS
110 120 130 140 150
KTDHKWSEFC HLVECDLKLL NEATHETSGA TEFFASEFNT KGSNTLIEYM
160 170 180 190 200
GPAPPKGSGP HRYVFLLYKQ PKGVDSSKFS KIKDRPNWGY GTPATGVGKW
210
AKENNLQLVA SNFFYAETK
Length:219
Mass (Da):24,357
Last modified:July 1, 1993 - v2
Checksum:i37D4FEC17677A573
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti56K → N in CAA44015 (PubMed:1745232).Curated1
Sequence conflicti165F → L in CAA33456 (PubMed:2693892).Curated1
Sequence conflicti211 – 219SNFFYAETK → VQFLLCGNEIGIYIYICICI YFLDFSAFHLTFYYFCFIYV FVTNGQMFVGTNVYVKQNT (PubMed:2693892).Curated9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15409 Genomic DNA. Translation: CAA33456.1.
X62105 Genomic DNA. Translation: CAA44015.1.
U17246 Genomic DNA. Translation: AAB67471.1.
BK006945 Genomic DNA. Translation: DAA09498.1.
PIRiS18843.
RefSeqiNP_013279.1. NM_001182065.1.

Genome annotation databases

EnsemblFungiiYLR178C; YLR178C; YLR178C.
GeneIDi850875.
KEGGisce:YLR178C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15409 Genomic DNA. Translation: CAA33456.1.
X62105 Genomic DNA. Translation: CAA44015.1.
U17246 Genomic DNA. Translation: AAB67471.1.
BK006945 Genomic DNA. Translation: DAA09498.1.
PIRiS18843.
RefSeqiNP_013279.1. NM_001182065.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WPXX-ray2.70B1-219[»]
ProteinModelPortaliP14306.
SMRiP14306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31449. 30 interactors.
DIPiDIP-669N.
IntActiP14306. 3 interactors.
MINTiMINT-2786931.

Protein family/group databases

MEROPSiI51.001.

PTM databases

iPTMnetiP14306.

Proteomic databases

MaxQBiP14306.
PRIDEiP14306.
TopDownProteomicsiP14306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYLR178C; YLR178C; YLR178C.
GeneIDi850875.
KEGGisce:YLR178C.

Organism-specific databases

EuPathDBiFungiDB:YLR178C.
SGDiS000004168. TFS1.

Phylogenomic databases

GeneTreeiENSGT00840000129869.
HOGENOMiHOG000237655.
InParanoidiP14306.
KOiK06910.
OMAiSGLHRYT.
OrthoDBiEOG092C48WA.

Enzyme and pathway databases

BioCyciYEAST:G3O-32303-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP14306.
PROiP14306.

Family and domain databases

Gene3Di3.90.280.10. 1 hit.
InterProiIPR008914. PEBP.
IPR001858. Phosphotidylethanolamine-bd_CS.
[Graphical view]
PfamiPF01161. PBP. 1 hit.
[Graphical view]
SUPFAMiSSF49777. SSF49777. 1 hit.
PROSITEiPS01220. PBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPYI_YEAST
AccessioniPrimary (citable) accession number: P14306
Secondary accession number(s): D6VYI2, P30312
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1030 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.