Reviewed, UniProtKB/Swiss-Prot P14293 (PMPB_CANBO)

Names and origin

Protein names

Recommended name:
    Putative peroxiredoxin-B
    EC=1.11.1.15
Alternative name(s):
    Thioredoxin reductase
    Peroxisomal membrane protein B
    PMP20
    Allergen=Cand b 2

Gene names

Name:

PMPB

Organism

Candida boidinii (Yeast)

Taxonomic identifier

5477 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Its function is very likely to be related to the metabolism of methanol.
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.
Subcellular location	Peroxisome membrane; Peripheral membrane protein.
Induction	By methanol.
Allergenic properties	Causes an allergic reaction in human. Shares common IgE-binding epitopes with allergen Asp f 3 of Aspergillus fumigatus.
Sequence similarities	Belongs to the peroxiredoxin 2 family. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Methanol utilization
Cellular component	Membrane Peroxisome
Disease	Allergen
Domain	Redox-active center
Molecular function	Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro methanol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisomal membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 167	166	Putative peroxiredoxin-B		PRO_0000056605
Regions
	Domain	4 – 167	164	Thioredoxin
	Motif	165 – 167	3	Microbody targeting signal

Sequences

	Sequence		Length	Mass (Da)
	P14293-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: A3909D7C6ACFBFD0	FASTA	167	18,056
	10 20 30 40 50 60 MAPIKRGDRF PTTDDVYYIP PEGGEPGAFE LSKFVKTKKF VVVSVPGAFT PPCTEQHLPG 70 80 90 100 110 120 YIKNLPRILS KGVDFVLVIT QNDPFVLKGW KKELGAADAK KLIFVSDPNL KLTKKLGSTI 130 140 150 160 DLSSIGLGTR SGRLALIVNR SGIVEYAAIE NGGEVDVSTA QKIIAKL

References

[1]	"Two genes encode the major membrane-associated protein of methanol-induced peroxisomes from Candida boidinii." Garrard L.J., Goodman J.M. J. Biol. Chem. 264:13929-13937(1989) [PubMed: 2760051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-26. Strain: ATCC 32195.
[2]	"Allergens of Aspergillus fumigatus and Candida boidinii share IgE-binding epitopes." Hemmann S., Blaser K., Crameri R. Am. J. Respir. Crit. Care Med. 156:1956-1962(1997) [PubMed: 9412580] [Abstract] Cited for: CROSS-REACTIVITY WITH ASP F 3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J04985 Genomic DNA. Translation: AAA34358.1.
PIR	B32646.
3D structure databases
HSSP	HSSP built from PDB template 1NM3 based on UniProtKB P44758.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.11.1.15. 675.
Family and domain databases
InterPro	IPR013740. Redoxin. IPR017936. Thioredoxin-like. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF08534. Redoxin. 1 hit. [Graphical view]
PROSITE	PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMPB_CANBO

Accession

Primary (citable) accession number: P14293

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	March 3, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)