P14290 (ERBS_SACEN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Erythromycin biosynthesis sensory transduction protein eryC1

Gene names

Name:	eryC1
Synonyms:	eryCI
Ordered Locus Names:	SACE_0734

Organism

Saccharopolyspora erythraea (strain NRRL 23338) [Complete proteome] [HAMAP]

Taxonomic identifier

405948 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Saccharopolyspora › Saccharopolyspora erythraea

Protein attributes

Sequence length	366 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Sensor protein that transfers the signal of environmental stimuli to the regulatory region of target genes to activate or repress transcription of erythromycin biosynthesis genes Potential.
Subcellular location	Cell membrane; Peripheral membrane protein Potential.
Sequence similarities	Belongs to the DegT/DnrJ/EryC1 family.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis Transcription Transcription regulation Two-component regulatory system
Cellular component	Cell membrane Membrane
Ligand	DNA-binding Pyridoxal phosphate
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay signal transduction system Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW catalytic activity Inferred from electronic annotation. Source: InterPro pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 366	366	Erythromycin biosynthesis sensory transduction protein eryC1		PRO_0000110012
Regions
	DNA binding	160 – 179	20	H-T-H motif Potential
	Region	53 – 158	106	Hydrophobic
Amino acid modifications
	Modified residue	186	1	N6-(pyridoxal phosphate)lysine By similarity
Experimental info
	Sequence conflict	69	1	L → V in CAA33548. Ref.1
	Sequence conflict	92 – 95	4	AVSR → GVP in CAA33548. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14290 [UniParc]. Last modified February 22, 2012. Version 2. Checksum: DAC755AB6890F279	FASTA	366	39,410
	10 20 30 40 50 60 MDVPFLDLQA AYLELRSDID QACRRVLGSG WYLHGPENEA FEAEFAAYCE NAHCVTVGSG 70 80 90 100 110 120 CDALELSLLA LGVGQGDEVI VPSHTFIATW LAVSRVGAVP VPVEPEGVSH TLDPALVEQA 130 140 150 160 170 180 ITPRTAAILP VHLYGHPADL DALRAIADRH GLALVEDVAQ AVGARHRGHR VGAGSNAAAF 190 200 210 220 230 240 SFYPGKNLGA LGDGGAVVTT DPALAERIRL LRNYGSKQKY VHEVRGTNAR LDELQAAVLR 250 260 270 280 290 300 VKLRHLDDWN ARRTTLAQHY QTELKDVPGI TLPETHPWAD SAWHLFVLRC ENRDHLQRHL 310 320 330 340 350 360 TDAGVQTLIH YPTPVHLSPA YADLGLPPGS FPVAESLAGE VLSLPIGPHL SREAADHVIA TLKAGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of a gene from Saccharopolyspora erythraea (Streptomyces erythraeus) which is involved in erythromycin biosynthesis." Dhillon N., Hale R.S., Cortes J., Leadlay P.F. Mol. Microbiol. 3:1405-1414(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NRRL 23338.
[2]	"Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338." Oliynyk M., Samborskyy M., Lester J.B., Mironenko T., Scott N., Dickens S., Haydock S.F., Leadlay P.F. Nat. Biotechnol. 25:447-453(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NRRL 23338.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15541 Genomic DNA. Translation: CAA33548.1. AM420293 Genomic DNA. Translation: CAM00075.1.
PIR	S06725.
RefSeq	YP_001103001.1. NC_009142.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	405948.SACE_0734.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM00075; CAM00075; SACE_0734.
GeneID	4940595.
KEGG	sen:SACE_0734.
PATRIC	23408856. VBISacEry28377_0748.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0399.
HOGENOM	HOG000230164.
OMA	AAYCEAK.
Family and domain databases
Gene3D	3.40.640.10. 1 hit. 3.90.1150.10. 1 hit.
InterPro	IPR000653. DegT/StrS_aminotransferase. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Pfam	PF01041. DegT_DnrJ_EryC1. 1 hit. [Graphical view]
PIRSF	PIRSF000390. PLP_StrS. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ERBS_SACEN

Accession

Primary (citable) accession number: P14290
Secondary accession number(s): A4F7Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 22, 2012
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program