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P14288 (BGAL_SULAC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Lactase
EC=3.2.1.23
Gene names
Name:bgaS
Ordered Locus Names:Saci_1849
OrganismSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]
Taxonomic identifier330779 [NCBI]
Taxonomic lineageArchaeaCrenarchaeotaThermoproteiSulfolobalesSulfolobaceaeSulfolobus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family.

Caution

Was originally (Ref.1) thought to originate from S.solfataricus strain P1, but the culture was contaminated with S.acidocaldarius.

Biophysicochemical properties

Temperature dependence:

Thermostable.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491Beta-galactosidase
PRO_0000063866

Sites

Active site2091Proton donor Potential
Active site3891Nucleophile By similarity

Experimental info

Sequence conflict1351Q → H in CAA34074. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14288 [UniParc].

Last modified August 30, 2005. Version 2.
Checksum: A7FF7F4DEEC1C189

FASTA49157,143
        10         20         30         40         50         60 
MLSFPKGFKF GWSQSGFQSE MGTPGSEDPN SDWHVWVHDR ENIVSQVVSG DLPENGPGYW 

        70         80         90        100        110        120 
GNYKRFHDEA EKIGLNAVRI NVEWSRIFPR PLPKPEMQTG TDKENSPVIS VDLNESKLRE 

       130        140        150        160        170        180 
MDNYANHEAL SHYRQILEDL RNRGFHIVLN MYHWTLPIWL HDPIRVRRGD FTGPTGWLNS 

       190        200        210        220        230        240 
RTVYEFARFS AYVAWKLDDL ASEYATMNEP NVVWGAGYAF PRAGFPPNYL SFRLSEIAKW 

       250        260        270        280        290        300 
NIIQAHARAY DAIKSVSKKS VGIIYANTSY YPLRPQDNEA VEIAERLNRW SFFDSIIKGE 

       310        320        330        340        350        360 
ITSEGQNVRE DLRNRLDWIG VNYYTRTVVT KAESGYLTLP GYGDRCERNS LSLANLPTSD 

       370        380        390        400        410        420 
FGWEFFPEGL YDVLLKYWNR YGLPLYVMEN GIADDADYQR PYYLVSHIYQ VHRALNEGVD 

       430        440        450        460        470        480 
VRGYLHWSLA DNYEWSSGFS MRFGLLKVDY LTKRLYWRPS ALVYREITRS NGIPEELEHL 

       490 
NRVPPIKPLR H 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of a thermostable beta-galactosidase from Sulfolobus solfataricus."
Little S., Cartwright P., Campbell C., Prenneta A., McChesney J., Mountain A., Robinson M.
Nucleic Acids Res. 17:7980-7980(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota."
Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A.
J. Bacteriol. 187:4992-4999(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15950 Genomic DNA. Translation: CAA34074.1.
CP000077 Genomic DNA. Translation: AAY81155.1.
RefSeqYP_256448.1. NC_007181.1.

3D structure databases

ProteinModelPortalP14288.
SMRP14288. Positions 1-491.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING330779.Saci_1849.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAY81155; AAY81155; Saci_1849.
GeneID3474772.
KEGGsai:Saci_1849.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
KOK01190.
OMAGEYTEMG.

Enzyme and pathway databases

BioCycSACI330779:GH9J-1818-MONOMER.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR018120. Glyco_hydro_1_AS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 2 hits.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00572. GLYCOSYL_HYDROL_F1_1. 1 hit.
PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_SULAC
AccessionPrimary (citable) accession number: P14288
Secondary accession number(s): Q4J7S6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: August 30, 2005
Last modified: May 14, 2014
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries