P14288 (BGAL_SULAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
Short name=Lactase
EC=3.2.1.23

Gene names

Name:	bgaS
Ordered Locus Names:	Saci_1849

Organism

Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) [Complete proteome] [HAMAP]

Taxonomic identifier

330779 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Caution	Was originally (Ref.1) thought to originate from S.solfataricus strain P1, but the culture was contaminated with S.acidocaldarius.
Biophysicochemical properties	Temperature dependence: Thermostable.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Beta-galactosidase		PRO_0000063866
Sites
	Active site	209	1	Proton donor Potential
	Active site	389	1	Nucleophile By similarity
Experimental info
	Sequence conflict	135	1	Q → H in CAA34074. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P14288 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: A7FF7F4DEEC1C189	FASTA	491	57,143
	10 20 30 40 50 60 MLSFPKGFKF GWSQSGFQSE MGTPGSEDPN SDWHVWVHDR ENIVSQVVSG DLPENGPGYW 70 80 90 100 110 120 GNYKRFHDEA EKIGLNAVRI NVEWSRIFPR PLPKPEMQTG TDKENSPVIS VDLNESKLRE 130 140 150 160 170 180 MDNYANHEAL SHYRQILEDL RNRGFHIVLN MYHWTLPIWL HDPIRVRRGD FTGPTGWLNS 190 200 210 220 230 240 RTVYEFARFS AYVAWKLDDL ASEYATMNEP NVVWGAGYAF PRAGFPPNYL SFRLSEIAKW 250 260 270 280 290 300 NIIQAHARAY DAIKSVSKKS VGIIYANTSY YPLRPQDNEA VEIAERLNRW SFFDSIIKGE 310 320 330 340 350 360 ITSEGQNVRE DLRNRLDWIG VNYYTRTVVT KAESGYLTLP GYGDRCERNS LSLANLPTSD 370 380 390 400 410 420 FGWEFFPEGL YDVLLKYWNR YGLPLYVMEN GIADDADYQR PYYLVSHIYQ VHRALNEGVD 430 440 450 460 470 480 VRGYLHWSLA DNYEWSSGFS MRFGLLKVDY LTKRLYWRPS ALVYREITRS NGIPEELEHL 490 NRVPPIKPLR H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a thermostable beta-galactosidase from Sulfolobus solfataricus." Little S., Cartwright P., Campbell C., Prenneta A., McChesney J., Mountain A., Robinson M. Nucleic Acids Res. 17:7980-7980(1989) [PubMed: 2508066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota." Chen L., Bruegger K., Skovgaard M., Redder P., She Q., Torarinsson E., Greve B., Awayez M., Zibat A., Klenk H.-P., Garrett R.A. J. Bacteriol. 187:4992-4999(2005) [PubMed: 15995215] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15950 Genomic DNA. Translation: CAA34074.1. CP000077 Genomic DNA. Translation: AAY81155.1.
RefSeq	YP_256448.1. NC_007181.1.
3D structure databases
ProteinModelPortal	P14288.
SMR	P14288. Positions 1-491.
ModBase	Search...
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3474772.
GenomeReviews	Gene locus Saci_1849 in contig CP000077_GR.
KEGG	sai:Saci_1849.
NMPDR	fig|330779.3.peg.1225.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG316462.
OMA	YYLVSHI.
ProtClustDB	CLSK865140.
Enzyme and pathway databases
BioCyc	SACI330779:SACI_1849-MONOMER.
Family and domain databases
InterPro	IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01190.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 2 hits. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_SULAC

Accession

Primary (citable) accession number: P14288
Secondary accession number(s): Q4J7S6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	August 30, 2005
Last modified:	November 16, 2011
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program