P14284 (DPOZ_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase zeta catalytic subunit
EC=2.7.7.7

Alternative name(s):
Protein reversionless 3

Gene names

Name:	REV3
Synonyms:	PSO1
Ordered Locus Names:	YPL167C
ORF Names:	P2535

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Nonessential DNA polymerase. Required for DNA damage induced mutagenesis. Involved in DNA repair, mitochondrial DNA repair and translesion synthesis. Translesion synthesis in S.cerevisiae may use a specialized DNA polymerase that is not required for other DNA replicative processes. Has a role in the bypass of abasic (AP) sites. Highly inefficient in incorporating nucleotides opposite the AP site, but efficiently extends from nucleotides, particularly an A, inserted opposite the lesion. Ref.1 Ref.5 Ref.6 Ref.7
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Ref.6
Subunit structure	Forms DNA polymerase zeta with REV7.
Subcellular location	Mitochondrion. Nucleus Potential Ref.7.
Sequence similarities	Belongs to the DNA polymerase type-B family.
Biophysicochemical properties	Kinetic parameters: KM=543 µM for dGTP (insertion opposite abasic site) Ref.6 KM=81 µM for dATP (insertion opposite abasic site) KM=125 µM for dTTP (insertion opposite abasic site) KM=113 µM for dCTP (insertion opposite abasic site) KM=0.11 µM for dGTP (insertion opposite C) KM=267 µM for dATP (insertion opposite C) KM=552 µM for dTTP (insertion opposite C) KM=321 µM for dCTP (insertion opposite C) KM=344 µM for dGTP (insertion opposite G) KM=118 µM for dATP (insertion opposite G) KM=428 µM for dTTP (insertion opposite G) KM=0.14 µM for dCTP (insertion opposite G) KM=6.5 µM for dTTP (extension from G, A, T, or C opposite abasic site) KM=2.3 µM for dTTP (extension from G, A, T, or C opposite abasic site) KM=49 µM for dTTP (extension from G, A, T, or C opposite abasic site) KM=21 µM for dTTP (extension from G, A, T, or C opposite abasic site) KM=0.35 µM for dTTP (extension from G, A, T, or C opposite C) KM=2.3 µM for dTTP (extension from G, A, T, or C opposite C) KM=15 µM for dTTP (extension from G, A, T, or C opposite C) KM=26 µM for dTTP (extension from G, A, T, or C opposite C) KM=6.9 µM for dTTP (extension from G, A, T, or C opposite G) KM=2.1 µM for dTTP (extension from G, A, T, or C opposite G) KM=28.8 µM for dTTP (extension from G, A, T, or C opposite G) KM=0.23 µM for dTTP (extension from G, A, T, or C opposite G)

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Mitochondrion Nucleus
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-free translesion synthesis Inferred from direct assay. Source: SGD error-prone translesion synthesis Inferred from direct assay. Source: SGD
Cellular component	mitochondrion Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell zeta DNA polymerase complex Inferred from direct assay Ref.5. Source: SGD
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from direct assay Ref.5. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1504	1504	DNA polymerase zeta catalytic subunit		PRO_0000046470
Regions
	Zinc finger	1398 – 1417	20	C4-type Potential
	Zinc finger	1446 – 1473	28	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	P14284 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 54C2C6B664F734F5	FASTA	1,504	172,957
	10 20 30 40 50 60 MSRESNDTIQ SDTVRSSSKS DYFRIQLNNQ DYYMSKPTFL DPSHGESLPL NQFSQVPNIR 70 80 90 100 110 120 VFGALPTGHQ VLCHVHGILP YMFIKYDGQI TDTSTLRHQR CAQVHKTLEV KIRASFKRKK 130 140 150 160 170 180 DDKHDLAGDK LGNLNFVADV SVVKGIPFYG YHVGWNLFYK ISLLNPSCLS RISELIRDGK 190 200 210 220 230 240 IFGKKFEIYE SHIPYLLQWT ADFNLFGCSW INVDRCYFRS PVLNSILDID KLTINDDLQL 250 260 270 280 290 300 LLDRFCDFKC NVLSRRDFPR VGNGLIEIDI LPQFIKNREK LQHRDIHHDF LEKLGDISDI 310 320 330 340 350 360 PVKPYVSSAR DMINELTMQR EELSLKEYKE PPETKRHVSG HQWQSSGEFE AFYKKAQHKT 370 380 390 400 410 420 STFDGQIPNF ENFIDKNQKF SAINTPYEAL PQLWPRLPQI EINNNSMQDK KNDDQVNASF 430 440 450 460 470 480 TEYEICGVDN ENEGVKGSNI KSRSYSWLPE SIASPKDSTI LLDHQTKYHN TINFSMDCAM 490 500 510 520 530 540 TQNMASKRKL RSSVSANKTS LLSRKRKKVM AAGLRYGKRA FVYGEPPFGY QDILNKLEDE 550 560 570 580 590 600 GFPKIDYKDP FFSNPVDLEN KPYAYAGKRF EISSTHVSTR IPVQFGGETV SVYNKPTFDM 610 620 630 640 650 660 FSSWKYALKP PTYDAVQKWY NKVPSMGNKK TESQISMHTP HSKFLYKFAS DVSGKQKRKK 670 680 690 700 710 720 SSVHDSLTHL TLEIHANTRS DKIPDPAIDE VSMIIWCLEE ETFPLDLDIA YEGIMIVHKA 730 740 750 760 770 780 SEDSTFPTKI QHCINEIPVM FYESEFEMFE ALTDLVLLLD PDILSGFEIH NFSWGYIIER 790 800 810 820 830 840 CQKIHQFDIV RELARVKCQI KTKLSDTWGY AHSSGIMITG RHMINIWRAL RSDVNLTQYT 850 860 870 880 890 900 IESAAFNILH KRLPHFSFES LTNMWNAKKS TTELKTVLNY WLSRAQINIQ LLRKQDYIAR 910 920 930 940 950 960 NIEQARLIGI DFHSVYYRGS QFKVESFLIR ICKSESFILL SPGKKDVRKQ KALECVPLVM 970 980 990 1000 1010 1020 EPESAFYKSP LIVLDFQSLY PSIMIGYNYC YSTMIGRVRE INLTENNLGV SKFSLPRNIL 1030 1040 1050 1060 1070 1080 ALLKNDVTIA PNGVVYAKTS VRKSTLSKML TDILDVRVMI KKTMNEIGDD NTTLKRLLNN 1090 1100 1110 1120 1130 1140 KQLALKLLAN VTYGYTSASF SGRMPCSDLA DSIVQTGRET LEKAIDIIEK DETWNAKVVY 1150 1160 1170 1180 1190 1200 GDTDSLFVYL PGKTAIEAFS IGHAMAERVT QNNPKPIFLK FEKVYHPSIL ISKKRYVGFS 1210 1220 1230 1240 1250 1260 YESPSQTLPI FDAKGIETVR RDGIPAQQKI IEKCIRLLFQ TKDLSKIKKY LQNEFFKIQI 1270 1280 1290 1300 1310 1320 GKVSAQDFCF AKEVKLGAYK SEKTAPAGAV VVKRRINEDH RAEPQYKERI PYLVVKGKQG 1330 1340 1350 1360 1370 1380 QLLRERCVSP EEFLEGENLE LDSEYYINKI LIPPLDRLFN LIGINVGNWA QEIVKSKRAS 1390 1400 1410 1420 1430 1440 TTTTKVENIT RVGTSATCCN CGEELTKICS LQLCDDCLEK RSTTTLSFLI KKLKRQKEYQ 1450 1460 1470 1480 1490 1500 TLKTVCRTCS YRYTSDAGIE NDHIASKCNS YDCPVFYSRV KAERYLRDNQ SVQREEALIS LNDW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"REV3, a Saccharomyces cerevisiae gene whose function is required for induced mutagenesis, is predicted to encode a nonessential DNA polymerase." Morrison A., Christensen R.B., Alley J., Beck A.K., Bernstine E.G., Lemontt J.F., Lawrence C.W. J. Bacteriol. 171:5659-5667(1989) [PubMed: 2676986] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"The sequence of 55 kb on the left arm of yeast chromosome XVI identifies a small nuclear RNA, a new putative protein kinase and two new putative regulators." Purnelle B., Coster F., Goffeau A. Yeast 12:1483-1492(1996) [PubMed: 8948103] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI." Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M. , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J. Nature 387:103-105(1997) [PubMed: 9169875] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Thymine-thymine dimer bypass by yeast DNA polymerase zeta." Nelson J.R., Lawrence C.W., Hinkle D.C. Science 272:1646-1649(1996) [PubMed: 8658138] [Abstract] Cited for: FUNCTION, INTERACTION WITH REV7.
[6]	"Roles of yeast DNA polymerases delta and zeta and of Rev1 in the bypass of abasic sites." Haracska L., Unk I., Johnson R.E., Johansson E., Burgers P.M.J., Prakash S., Prakash L. Genes Dev. 15:945-954(2001) [PubMed: 11316789] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
[7]	"Saccharomyces cerevisiae polymerase zeta functions in mitochondria." Zhang H., Chatterjee A., Singh K.K. Genetics 172:2683-2688(2006) [PubMed: 16452144] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29683 Genomic DNA. Translation: AAA34968.1. X96770 Genomic DNA. Translation: CAA65554.1. Z73523 Genomic DNA. Translation: CAA97873.1. BK006949 Genomic DNA. Translation: DAA11267.1.
PIR	A33602.
RefSeq	NP_015158.1. NM_001183981.1.
3D structure databases
ProteinModelPortal	P14284.
SMR	P14284. Positions 666-1395.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14284. 1 interaction.
MINT	MINT-2731570.
STRING	P14284.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YPL167C; YPL167C; YPL167C.
GeneID	855936.
KEGG	sce:YPL167C.
NMPDR	fig|4932.3.peg.6287.
Organism-specific databases
CYGD	YPL167c.
SGD	S000006088. REV3.
Phylogenomic databases
eggNOG	fuNOG05563.
GeneTree	EFGT00050000004684.
HOGENOM	HBG396797.
OMA	DRWGYTH.
OrthoDB	EOG4FN7RT.
Gene expression databases
ArrayExpress	P14284.
Genevestigator	P14284.
GermOnline	YPL167C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006172. DNA-dir_DNA_pol_B. IPR017964. DNA-dir_DNA_pol_B_CS. IPR006133. DNA-dir_DNA_pol_B_exonuc. IPR006134. DNA-dir_DNA_pol_B_multi_dom. IPR023211. DNA_pol_palm_dom. IPR012337. RNaseH-like_dom. [Graphical view]
Gene3D	G3DSA:3.90.1600.10. DNA_pol_palm_dom. 2 hits.
KO	K02350.
Pfam	PF00136. DNA_pol_B. 1 hit. PF03104. DNA_pol_B_exo1. 2 hits. [Graphical view]
PRINTS	PR00106. DNAPOLB.
SMART	SM00486. POLBc. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
PROSITE	PS00116. DNA_POLYMERASE_B. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	980687.

Entry information

Entry name

DPOZ_YEAST

Accession

Primary (citable) accession number: P14284
Secondary accession number(s): D6W3K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	December 14, 2011
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program