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Protein

Pertactin autotransporter

Gene

prn

Organism
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Agglutinogen that binds to eukaryotic cells; a process mediated by the R-G-D sequence. Pertactin may have a role in bacterial adhesion, and thus play a role in virulence. May contribute to the disease state of whooping cough.

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Virulence

Enzyme and pathway databases

BioCyciBPER257313:BP1054-MONOMER.

Protein family/group databases

TCDBi1.B.12.2.1. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Pertactin autotransporter
Alternative name(s):
P.93
Cleaved into the following 2 chains:
Gene namesi
Name:prn
Synonyms:omp69A
Ordered Locus Names:BP1054
OrganismiBordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Taxonomic identifieri257313 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaeBordetella
Proteomesi
  • UP000002676 Componenti: Chromosome

Subcellular locationi

Pertactin translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3434Add
BLAST
Chaini35 – 910876Pertactin autotransporterPRO_0000002705Add
BLAST
Chaini35 – 631597Outer membrane protein P.69PRO_0000002706Add
BLAST
Chaini632 – 910279Pertactin translocatorPRO_0000002707Add
BLAST

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei631 – 6322CleavageBy similarity

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi257313.BP1054.

Structurei

Secondary structure

1
910
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi40 – 434Combined sources
Beta strandi50 – 534Combined sources
Beta strandi60 – 667Combined sources
Beta strandi68 – 714Combined sources
Beta strandi78 – 9215Combined sources
Beta strandi94 – 974Combined sources
Beta strandi104 – 1074Combined sources
Beta strandi112 – 12413Combined sources
Beta strandi126 – 1294Combined sources
Turni133 – 1353Combined sources
Beta strandi139 – 1457Combined sources
Beta strandi148 – 1547Combined sources
Beta strandi156 – 1594Combined sources
Beta strandi162 – 1665Combined sources
Beta strandi170 – 1756Combined sources
Beta strandi183 – 1864Combined sources
Beta strandi199 – 2057Combined sources
Beta strandi207 – 2104Combined sources
Beta strandi217 – 23721Combined sources
Beta strandi239 – 24810Combined sources
Beta strandi250 – 26011Combined sources
Beta strandi266 – 2705Combined sources
Beta strandi281 – 2833Combined sources
Beta strandi294 – 30916Combined sources
Beta strandi311 – 3133Combined sources
Beta strandi316 – 3249Combined sources
Beta strandi328 – 34720Combined sources
Beta strandi354 – 3563Combined sources
Beta strandi359 – 3646Combined sources
Beta strandi368 – 37710Combined sources
Beta strandi383 – 3875Combined sources
Beta strandi392 – 3987Combined sources
Beta strandi403 – 4053Combined sources
Helixi407 – 4093Combined sources
Beta strandi413 – 4186Combined sources
Beta strandi422 – 4254Combined sources
Beta strandi430 – 4378Combined sources
Beta strandi439 – 4424Combined sources
Beta strandi446 – 4549Combined sources
Beta strandi458 – 4614Combined sources
Beta strandi472 – 4809Combined sources
Beta strandi483 – 4897Combined sources
Helixi490 – 4923Combined sources
Beta strandi497 – 5048Combined sources
Beta strandi506 – 5149Combined sources
Beta strandi525 – 5295Combined sources
Beta strandi537 – 5404Combined sources
Helixi543 – 5453Combined sources
Beta strandi546 – 5494Combined sources
Beta strandi552 – 5576Combined sources
Beta strandi560 – 5667Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DABX-ray2.50A35-573[»]
ProteinModelPortaliP14283.
SMRiP14283. Positions 35-573.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14283.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati266 – 27051
Repeati271 – 27552
Repeati276 – 28053
Repeati281 – 28554; approximate
Repeati286 – 29055; approximate
Domaini642 – 910269AutotransporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni266 – 290255 X 5 AA tandem repeats of G-G-A-V-PAdd
BLAST
Regioni579 – 593155 X 3 AA tandem repeats of P-Q-PAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi260 – 2623Cell attachment site; involved in adhesion to various eukaryotic cell lines

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108P8G. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000115499.
KOiK12681.
OMAiYATYIAN.
OrthoDBiEOG6GR352.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR003992. Pertactin.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01482. PERTACTIN.
PR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNMSLSRIVK AAPLRRTTLA MALGALGAAP AAHADWNNQS IVKTGERQHG
60 70 80 90 100
IHIQGSDPGG VRTASGTTIK VSGRQAQGIL LENPAAELQF RNGSVTSSGQ
110 120 130 140 150
LSDDGIRRFL GTVTVKAGKL VADHATLANV GDTWDDDGIA LYVAGEQAQA
160 170 180 190 200
SIADSTLQGA GGVQIERGAN VTVQRSAIVD GGLHIGALQS LQPEDLPPSR
210 220 230 240 250
VVLRDTNVTA VPASGAPAAV SVLGASELTL DGGHITGGRA AGVAAMQGAV
260 270 280 290 300
VHLQRATIRR GDAPAGGAVP GGAVPGGAVP GGFGPGGFGP VLDGWYGVDV
310 320 330 340 350
SGSSVELAQS IVEAPELGAA IRVGRGARVT VSGGSLSAPH GNVIETGGAR
360 370 380 390 400
RFAPQAAPLS ITLQAGAHAQ GKALLYRVLP EPVKLTLTGG ADAQGDIVAT
410 420 430 440 450
ELPSIPGTSI GPLDVALASQ ARWTGATRAV DSLSIDNATW VMTDNSNVGA
460 470 480 490 500
LRLASDGSVD FQQPAEAGRF KVLTVNTLAG SGLFRMNVFA DLGLSDKLVV
510 520 530 540 550
MQDASGQHRL WVRNSGSEPA SANTLLLVQT PLGSAATFTL ANKDGKVDIG
560 570 580 590 600
TYRYRLAANG NGQWSLVGAK APPAPKPAPQ PGPQPPQPPQ PQPEAPAPQP
610 620 630 640 650
PAGRELSAAA NAAVNTGGVG LASTLWYAES NALSKRLGEL RLNPDAGGAW
660 670 680 690 700
GRGFAQRQQL DNRAGRRFDQ KVAGFELGAD HAVAVAGGRW HLGGLAGYTR
710 720 730 740 750
GDRGFTGDGG GHTDSVHVGG YATYIADSGF YLDATLRASR LENDFKVAGS
760 770 780 790 800
DGYAVKGKYR THGVGASLEA GRRFTHADGW FLEPQAELAV FRAGGGAYRA
810 820 830 840 850
ANGLRVRDEG GSSVLGRLGL EVGKRIELAG GRQVQPYIKA SVLQEFDGAG
860 870 880 890 900
TVHTNGIAHR TELRGTRAEL GLGMAAALGR GHSLYASYEY SKGPKLAMPW
910
TFHAGYRYSW
Length:910
Mass (Da):93,453
Last modified:February 1, 1994 - v3
Checksum:iA169871E20A2E7DB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04560 Genomic DNA. Translation: AAA22980.1. Sequence problems.
BX640414 Genomic DNA. Translation: CAE41353.1.
RefSeqiNP_879839.1. NC_002929.2.
WP_010930159.1. NC_002929.2.

Genome annotation databases

EnsemblBacteriaiCAE41353; CAE41353; BP1054.
GeneIDi2664290.
KEGGibpe:BP1054.
PATRICi21155252. VBIBorPer7866_1126.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04560 Genomic DNA. Translation: AAA22980.1. Sequence problems.
BX640414 Genomic DNA. Translation: CAE41353.1.
RefSeqiNP_879839.1. NC_002929.2.
WP_010930159.1. NC_002929.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DABX-ray2.50A35-573[»]
ProteinModelPortaliP14283.
SMRiP14283. Positions 35-573.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi257313.BP1054.

Protein family/group databases

TCDBi1.B.12.2.1. the autotransporter-1 (at-1) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAE41353; CAE41353; BP1054.
GeneIDi2664290.
KEGGibpe:BP1054.
PATRICi21155252. VBIBorPer7866_1126.

Phylogenomic databases

eggNOGiENOG4108P8G. Bacteria.
COG3468. LUCA.
HOGENOMiHOG000115499.
KOiK12681.
OMAiYATYIAN.
OrthoDBiEOG6GR352.

Enzyme and pathway databases

BioCyciBPER257313:BP1054-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP14283.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR003992. Pertactin.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01482. PERTACTIN.
PR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis."
    Charles I.G., Dougan G., Pickard D., Chatfield S., Smith M., Novotny P., Morrissey P., Fairweather N.F.
    Proc. Natl. Acad. Sci. U.S.A. 86:3554-3558(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    Strain: CN2992.
  2. "Cloning, nucleotide sequence and heterologous expression of the protective outer-membrane protein P.68 pertactin from Bordetella bronchiseptica."
    Li J.L., Fairweather N.F., Novotny P., Dougan G., Charles I.G.
    J. Gen. Microbiol. 138:1697-1705(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 264 AND 332.
  3. "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica."
    Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S.
    , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J.
    Nat. Genet. 35:32-40(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tohama I / ATCC BAA-589 / NCTC 13251.
  4. "Crystallization and preliminary X-ray diffraction analysis of P30, the transmembrane domain of pertactin, an autotransporter from Bordetella pertussis."
    Zhu Y., Black I., Roszak A.W., Isaacs N.W.
    Acta Crystallogr. F 63:593-595(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  5. "Structure of Bordetella pertussis virulence factor P.69 pertactin."
    Emsley P., Charles I.G., Fairweather N.F., Isaacs N.W.
    Nature 381:90-92(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

Entry informationi

Entry nameiPERT_BORPE
AccessioniPrimary (citable) accession number: P14283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1994
Last modified: November 11, 2015
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Synthesized only in the presence of low Mg2+ concentration.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.