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Protein

Plasma kallikrein

Gene

Klkb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Catalytic activityi

Cleaves selectively Arg-|-Xaa and Lys-|-Xaa bonds, including Lys-|-Arg and Arg-|-Ser bonds in (human) kininogen to release bradykinin.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei434Charge relay system1
Active sitei483Charge relay system1
Active sitei578Charge relay system1

GO - Molecular functioni

GO - Biological processi

  • blood coagulation, intrinsic pathway Source: InterPro
  • fibrinolysis Source: UniProtKB-KW
  • inflammatory response Source: UniProtKB-KW
  • liver regeneration Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processBlood coagulation, Fibrinolysis, Hemostasis, Inflammatory response

Enzyme and pathway databases

BRENDAi3.4.21.34 5301

Protein family/group databases

MEROPSiS01.212

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma kallikrein (EC:3.4.21.34)
Alternative name(s):
Fletcher factor
Kininogenin
Plasma prekallikrein
Cleaved into the following 2 chains:
Gene namesi
Name:Klkb1
Synonyms:Klk3, Pk
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi67382 Klkb1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002802520 – 390Plasma kallikrein heavy chainAdd BLAST371
ChainiPRO_0000028026391 – 638Plasma kallikrein light chainAdd BLAST248

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi21 ↔ 104By similarity
Disulfide bondi47 ↔ 77By similarity
Disulfide bondi51 ↔ 57By similarity
Disulfide bondi111 ↔ 194By similarity
Glycosylationi127N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi137 ↔ 166By similarity
Disulfide bondi141 ↔ 147By similarity
Disulfide bondi201 ↔ 284By similarity
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi227 ↔ 256By similarity
Disulfide bondi231 ↔ 237By similarity
Disulfide bondi292 ↔ 375By similarity
Glycosylationi308N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi318 ↔ 347By similarity
Disulfide bondi322 ↔ 328By similarity
Disulfide bondi340 ↔ 345By similarity
Disulfide bondi383 ↔ 503By similarity
Glycosylationi396N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi419 ↔ 435By similarity
Glycosylationi453N-linked (GlcNAc...) asparagineCurated1
Glycosylationi459N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi494N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi517 ↔ 584By similarity
Disulfide bondi548 ↔ 563By similarity
Disulfide bondi574 ↔ 602By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP14272
PRIDEiP14272

PTM databases

iPTMnetiP14272
PhosphoSitePlusiP14272

Interactioni

Subunit structurei

Forms a heterodimer with SERPINA5. The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000019237

Structurei

3D structure databases

ProteinModelPortaliP14272
SMRiP14272
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 104Apple 1PROSITE-ProRule annotationAdd BLAST84
Domaini111 – 194Apple 2PROSITE-ProRule annotationAdd BLAST84
Domaini201 – 284Apple 3PROSITE-ProRule annotationAdd BLAST84
Domaini292 – 375Apple 4PROSITE-ProRule annotationAdd BLAST84
Domaini391 – 626Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Belongs to the peptidase S1 family. Plasma kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOGENOMiHOG000112467
HOVERGENiHBG000399
InParanoidiP14272
PhylomeDBiP14272

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR000177 Apple
IPR003609 Pan_app
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR034813 Plasma_kallikrein
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR44459:SF2 PTHR44459:SF2, 1 hit
PfamiView protein in Pfam
PF00024 PAN_1, 4 hits
PF00089 Trypsin, 1 hit
PRINTSiPR00005 APPLEDOMAIN
PR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00223 APPLE, 4 hits
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS00495 APPLE, 4 hits
PS50948 PAN, 4 hits
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14272-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILFKQVGYF VSLFATVSCG CLSQLYANTF FRGGDLAAIY TPDAQHCQKM
60 70 80 90 100
CTFHPRCLLF SFLAVSPTKE TDKRFGCFMK ESITGTLPRI HRTGAISGHS
110 120 130 140 150
LKQCGHQLSA CHQDIYEGLD MRGSNFNISK TDSIEECQKL CTNNIHCQFF
160 170 180 190 200
TYATKAFHRP EYRKSCLLKR SSSGTPTSIK PVDNLVSGFS LKSCALSEIG
210 220 230 240 250
CPMDIFQHFA FADLNVSQVV TPDAFVCRTV CTFHPNCLFF TFYTNEWETE
260 270 280 290 300
SQRNVCFLKT SKSGRPSPPI IQENAVSGYS LFTCRKARPE PCHFKIYSGV
310 320 330 340 350
AFEGEELNAT FVQGADACQE TCTKTIRCQF FTYSLLPQDC KAEGCKCSLR
360 370 380 390 400
LSTDGSPTRI TYEAQGSSGY SLRLCKVVES SDCTTKINAR IVGGTNSSLG
410 420 430 440 450
EWPWQVSLQV KLVSQNHMCG GSIIGRQWIL TAAHCFDGIP YPDVWRIYGG
460 470 480 490 500
ILNLSEITNK TPFSSIKELI IHQKYKMSEG SYDIALIKLQ TPLNYTEFQK
510 520 530 540 550
PICLPSKADT NTIYTNCWVT GWGYTKERGE TQNILQKATI PLVPNEECQK
560 570 580 590 600
KYRDYVITKQ MICAGYKEGG IDACKGDSGG PLVCKHSGRW QLVGITSWGE
610 620 630
GCARKEQPGV YTKVAEYIDW ILEKIQSSKE RALETSPA
Length:638
Mass (Da):71,274
Last modified:January 1, 1990 - v1
Checksum:i454BEB27E8CA8F88
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62357
, M62358, M62346, M62347, M62349, M62350, M62351, M62352, M62353, M62354, M62355, M62356 Genomic DNA Translation: AAA74563.1
M30282 mRNA Translation: AAA41463.1
M58590 mRNA Translation: AAA42069.1
PIRiA39180 KQRTPL
UniGeneiRn.9880

Similar proteinsi

Entry informationi

Entry nameiKLKB1_RAT
AccessioniPrimary (citable) accession number: P14272
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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