Plasma kallikrein precursor - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P14272 (KLKB1_RAT)

Last modified June 16, 2009. Version 88. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Plasma kallikrein
    EC=3.4.21.34
Alternative name(s):
    Plasma prekallikrein
    Kininogenin
    Fletcher factor
Cleaved into the following 2 chains:
    1- Recommended name:
            Plasma kallikrein heavy chain
    2- Recommended name:
            Plasma kallikrein light chain

Gene names

Name:	Klkb1
Synonyms:	Klk3, Pk

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Catalytic activity	Cleaves selectively Arg-\|-Xaa and Lys-\|-Xaa bonds, including Lys-\|-Arg and Arg-\|-Ser bonds in (human) kininogen to release bradykinin.
Subunit structure	The zymogen is activated by factor XIIa, which cleaves the molecule into a light chain, which contains the active site, and a heavy chain, which associates with HMW kininogen. These chains are linked by one or more disulfide bonds.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase S1 family. Plasma kallikrein subfamily. Contains 4 apple domains. Contains 1 peptidase S1 domain.

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Ontologies

Keywords
Biological process	Blood coagulation Fibrinolysis Inflammatory response
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW fibrinolysis Inferred from electronic annotation. Source: UniProtKB-KW inflammatory response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

Chain

20 – 390

371

Plasma kallikrein heavy chain

PRO_0000028025

Chain

391 – 638

248

Plasma kallikrein light chain

PRO_0000028026

Regions

Domain

21 – 104

Apple 1

Domain

111 – 194

Apple 2

Domain

201 – 284

Apple 3

Domain

292 – 375

Apple 4

Domain

391 – 626

236

Peptidase S1

Sites

Active site

434

Charge relay system

Active site

483

Charge relay system

Active site

578

Charge relay system

Amino acid modifications

Glycosylation

127

N-linked (GlcNAc...) Probable

Glycosylation

215

N-linked (GlcNAc...) Potential

Glycosylation

308

N-linked (GlcNAc...) Probable

Glycosylation

396

N-linked (GlcNAc...) Ref.2

Glycosylation

453

N-linked (GlcNAc...) Probable

Glycosylation

459

N-linked (GlcNAc...) Potential

Glycosylation

494

N-linked (GlcNAc...) Probable

Disulfide bond

21 ↔ 104

By similarity

Disulfide bond

47 ↔ 77

By similarity

Disulfide bond

51 ↔ 57

By similarity

Disulfide bond

111 ↔ 194

By similarity

Disulfide bond

137 ↔ 166

By similarity

Disulfide bond

141 ↔ 147

By similarity

Disulfide bond

201 ↔ 284

By similarity

Disulfide bond

227 ↔ 256

By similarity

Disulfide bond

231 ↔ 237

By similarity

Disulfide bond

292 ↔ 375

By similarity

Disulfide bond

318 ↔ 347

By similarity

Disulfide bond

322 ↔ 328

By similarity

Disulfide bond

340 ↔ 345

By similarity

Disulfide bond

383 ↔ 503

By similarity

Disulfide bond

419 ↔ 435

By similarity

Disulfide bond

517 ↔ 584

By similarity

Disulfide bond

548 ↔ 563

By similarity

Disulfide bond

574 ↔ 602

By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P14272-1 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 454BEB27E8CA8F88
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FASTA

638

71,274

        10         20         30         40         50         60 
MILFKQVGYF VSLFATVSCG CLSQLYANTF FRGGDLAAIY TPDAQHCQKM CTFHPRCLLF 

        70         80         90        100        110        120 
SFLAVSPTKE TDKRFGCFMK ESITGTLPRI HRTGAISGHS LKQCGHQLSA CHQDIYEGLD 

       130        140        150        160        170        180 
MRGSNFNISK TDSIEECQKL CTNNIHCQFF TYATKAFHRP EYRKSCLLKR SSSGTPTSIK 

       190        200        210        220        230        240 
PVDNLVSGFS LKSCALSEIG CPMDIFQHFA FADLNVSQVV TPDAFVCRTV CTFHPNCLFF 

       250        260        270        280        290        300 
TFYTNEWETE SQRNVCFLKT SKSGRPSPPI IQENAVSGYS LFTCRKARPE PCHFKIYSGV 

       310        320        330        340        350        360 
AFEGEELNAT FVQGADACQE TCTKTIRCQF FTYSLLPQDC KAEGCKCSLR LSTDGSPTRI 

       370        380        390        400        410        420 
TYEAQGSSGY SLRLCKVVES SDCTTKINAR IVGGTNSSLG EWPWQVSLQV KLVSQNHMCG 

       430        440        450        460        470        480 
GSIIGRQWIL TAAHCFDGIP YPDVWRIYGG ILNLSEITNK TPFSSIKELI IHQKYKMSEG 

       490        500        510        520        530        540 
SYDIALIKLQ TPLNYTEFQK PICLPSKADT NTIYTNCWVT GWGYTKERGE TQNILQKATI 

       550        560        570        580        590        600 
PLVPNEECQK KYRDYVITKQ MICAGYKEGG IDACKGDSGG PLVCKHSGRW QLVGITSWGE 

       610        620        630 
GCARKEQPGV YTKVAEYIDW ILEKIQSSKE RALETSPA

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References

[1]	"Gene structure and chromosomal localization of plasma kallikrein." Beaubien G., Rosinski-Chupin I., Mattei M.-G., Mbikay M., Chretien M., Seidah N.G. Biochemistry 30:1628-1635(1991) [PubMed: 1993180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The cDNA structure of rat plasma kallikrein." Seidah N.G., Ladenheim R., Mbikay M., Hamelin J., Lutfalla G., Rougeon F., Lazure C., Chretien M. DNA 8:563-574(1989) [PubMed: 2598771] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION AT ASN-396.

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Cross-references

Sequence databases
	M62357 M62356 Genomic DNA. Translation: AAA74563.1. M30282 mRNA. Translation: AAA41463.1. M58590 mRNA. Translation: AAA42069.1.
IPI	IPI00203384.
PIR	KQRTPL. A39180.
UniGene	Rn.9880
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
ModBase	Search...
Protein family/group databases
MEROPS	S01.212.
Proteomic databases
PRIDE	P14272.
Genome annotation databases
Ensembl	ENSRNOG00000014118. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	2971. Klk3.
Phylogenomic databases
HOVERGEN	P14272.
Enzyme and pathway databases
BRENDA	3.4.21.34. 248.
Gene expression databases
ArrayExpress	P14272.
GermOnline	ENSRNOG00000014118. Rattus norvegicus.
Family and domain databases
InterPro	IPR000177. Apple. IPR003014. PAN-1_domain. IPR003609. Pan_app. IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00024. PAN_1. 4 hits. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00005. APPLEDOMAIN. PR00722. CHYMOTRYPSIN.
SMART	SM00223. APPLE. 4 hits. SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS00495. APPLE. 4 hits. PS50948. PAN. 4 hits. PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

KLKB1_RAT

Accession

Primary (citable) accession number: P14272

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents