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Protein

Mitochondrial brown fat uncoupling protein 1

Gene

UCP1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.By similarity

Enzyme regulationi

Has no constitutive proton transporter activity and has to be activated by long-chain fatty acids/LCFAs. Inhibited by purine nucleotides. Both purine nucleotides and LCFAs bind the cytosolic side of the transporter and directly compete to activate or inhibit it. Activated by noradrenaline and reactive oxygen species.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial brown fat uncoupling protein 1Curated
Short name:
UCP 1Curated
Alternative name(s):
Solute carrier family 25 member 7By similarity
ThermogeninBy similarity
Gene namesi
Name:UCP1By similarity
Synonyms:SLC25A7By similarity, UCP1 Publication
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 10Mitochondrial intermembraneBy similarity9
Transmembranei11 – 32Helical; Name=1Sequence analysisAdd BLAST22
Topological domaini33 – 73Mitochondrial matrixBy similarityAdd BLAST41
Transmembranei74 – 96Helical; Name=2Sequence analysisAdd BLAST23
Topological domaini97 – 115Mitochondrial intermembraneBy similarityAdd BLAST19
Transmembranei116 – 132Helical; Name=3Sequence analysisAdd BLAST17
Topological domaini133 – 177Mitochondrial matrixBy similarityAdd BLAST45
Transmembranei178 – 194Helical; Name=4Sequence analysisAdd BLAST17
Topological domaini195 – 211Mitochondrial intermembraneBy similarityAdd BLAST17
Transmembranei212 – 231Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini232 – 265Mitochondrial matrixBy similarityAdd BLAST34
Transmembranei266 – 288Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini289 – 306Mitochondrial intermembraneBy similarityAdd BLAST18

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000906612 – 306Mitochondrial brown fat uncoupling protein 1Add BLAST305

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei253Cysteine sulfenic acid (-SOH)By similarity1

Post-translational modificationi

May undergo sulfenylation upon cold exposure. May increase the sensitivity of UCP1 thermogenic function to the activation by noradrenaline probably through structural effects.By similarity
May undergo ubiquitin-mediated proteasomal degradation.By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiP14271.

Expressioni

Tissue specificityi

Brown adipose tissue.1 Publication

Interactioni

Subunit structurei

Most probably functions as a monomer. Binds one purine nucleotide per monomer. However, has also been suggested to function as a homodimer or a homotetramer. Tightly associates with cardiolipin in the mitochondrion inner membrane; may stabilize and regulate its activity.By similarity

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000001977.

Structurei

3D structure databases

ProteinModelPortaliP14271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 102Solcar 1Add BLAST92
Repeati110 – 200Solcar 2Add BLAST91
Repeati209 – 294Solcar 3Add BLAST86

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP14271.
KOiK08769.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14271-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGTTTTDVP PTMGVKIFSA GVAACLADVI TFPLDTAKVR QQIQGEFPIT
60 70 80 90 100
SGIRYKGVLG TITTLAKTEG PLKLYSGLPA GLQRQISFAS LRIGLYDTVQ
110 120 130 140 150
EFFTSGEETP SLGSKISAGL TTGGVAVFIG QPTEVVKVRL QAQSHLHGLK
160 170 180 190 200
PRYTGTYNAY RIIATTESLT SLWKGTTPNL LRNVIINCTE LVTYDLMKGA
210 220 230 240 250
LVRNEILADD VPCHFVSALI AGFCTTLLSS PVDVVKTRFI NSPPGQYASV
260 270 280 290 300
PNCAMTMFTK EGPTAFFKGF VPSFLRLGSW NVIMFVCFEK LKGELMRSRQ

TVDCAT
Length:306
Mass (Da):33,083
Last modified:January 1, 1990 - v1
Checksum:i58343CAD94C910F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14696 mRNA. Translation: CAA32826.1.
PIRiA32446.
RefSeqiNP_001164548.1. NM_001171077.1.
UniGeneiOcu.2093.

Genome annotation databases

GeneIDi100328618.
KEGGiocu:100328618.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14696 mRNA. Translation: CAA32826.1.
PIRiA32446.
RefSeqiNP_001164548.1. NM_001171077.1.
UniGeneiOcu.2093.

3D structure databases

ProteinModelPortaliP14271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9986.ENSOCUP00000001977.

Proteomic databases

PRIDEiP14271.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328618.
KEGGiocu:100328618.

Organism-specific databases

CTDi7350.

Phylogenomic databases

eggNOGiKOG0753. Eukaryota.
ENOG410XRV1. LUCA.
HOGENOMiHOG000165140.
HOVERGENiHBG009528.
InParanoidiP14271.
KOiK08769.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002030. Mit_uncoupling.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00784. MTUNCOUPLING.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUCP1_RABIT
AccessioniPrimary (citable) accession number: P14271
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.