P14263 (CP51_CANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lanosterol 14-alpha demethylase
EC=1.14.13.70

Alternative name(s):
CYPLI
Cytochrome P450 51
Cytochrome P450-14DM
Cytochrome P450-LIA1
Sterol 14-alpha demethylase

Gene names

Name:	ERG11
Synonyms:	CYP51

Organism

Candida tropicalis (Yeast)

Taxonomic identifier

5482 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	528 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Membrane Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 528	528	Lanosterol 14-alpha demethylase		PRO_0000052005
Sites
	Metal binding	470	1	Iron (heme axial ligand)
Experimental info
	Sequence conflict	448	1	G → V in AAA34316. Ref.2
	Sequence conflict	500	1	V → G in AAA34316. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14263 [UniParc]. Last modified November 1, 1990. Version 2. Checksum: 83F8E3C1C1E826B9	FASTA	528	60,928
	10 20 30 40 50 60 MAIVDTAIDG INYFLSLSLT QQITILVVFP FIYNIAWQLL YSLRKDRVPM VFYWIPWFGS 70 80 90 100 110 120 AASYGMQPYE FFEKCRLKYG DVFSFMLLGK VMTVYLGPKG HEFIYNAKLS DVSAEEAYTH 130 140 150 160 170 180 LTTPVFGKGV IYDCPNSRLM EQKKFAKFAL TTDSFKTYVP KIREEVLNYF VNDVSFKTKE 190 200 210 220 230 240 RDHGVASVMK TQPEITIFTA SRCLFGDEMR KSFDRSFAQL YADLDKGFTP INFVFPNLPL 250 260 270 280 290 300 PHYWRRDAAQ RKISAHYMKE IKRRRESGDI DPKRDLIDSL LVNSTYKDGV KMTDQEIANL 310 320 330 340 350 360 LIGVLMGGQH TSASTSAWFL LHLAEQPQLQ DDLYEELTNL LKEKGGDLND LTYEDLQKLP 370 380 390 400 410 420 LVNNTIKETL RMHMPLHSIF RKVMNPLRVP NTKYVIPKGH YVLVSAGYAH TSDRWFEHPE 430 440 450 460 470 480 HFNPRRWESD DTKASAVSFN SEDTVDYGFG KISKGVSSPY LPFGGGRHRC IGEQFAYVQL 490 500 510 520 GTILTTYIYN FKWRLNGDKV PDVDYQSMVT LPLEPAEIVW EKRDTCMV

References

[1]	"Primary structure of the cytochrome P450 lanosterol 14 alpha-demethylase gene from Candida tropicalis." Chen C., Kalb V.F., Turi T.G., Loper J.C. DNA 7:617-626(1988) [PubMed: 3068024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 750 / CBS 94 / DSM 11953 / JCM 1541 / NBRC 1400.
[2]	"Isolation of the Candida tropicalis gene for P450 lanosterol demethylase and its expression in Saccharomyces cerevisiae." Chen C., Turi T.G., Sanglard D., Loper J.C. Biochem. Biophys. Res. Commun. 146:1311-1317(1987) [PubMed: 3304292] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 434-528. Strain: ATCC 750 / CBS 94 / DSM 11953 / JCM 1541 / NBRC 1400.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M23673 Genomic DNA. Translation: AAA53284.1. M17595 Genomic DNA. Translation: AAA34316.1.
PIR	A31854.
3D structure databases
ProteinModelPortal	P14263.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
PhylomeDB	P14263.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51_CANTR

Accession

Primary (citable) accession number: P14263

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1990
Last modified:	September 21, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program