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P14250

- GUN3_FIBSS

UniProt

P14250 - GUN3_FIBSS

Protein

Endoglucanase 3

Gene

cel-3

Organism
Fibrobacter succinogenes (strain ATCC 19169 / S85)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Exhibits both endoglucanase and cellobiosidase activities.

    Catalytic activityi

    Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei448 – 4481Proton donorBy similarity
    Active sitei597 – 5971NucleophileBy similarity

    GO - Molecular functioni

    1. cellulase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellulose catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

    Protein family/group databases

    CAZyiGH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoglucanase 3 (EC:3.2.1.4)
    Alternative name(s):
    Cellulase 3
    Endo-1,4-beta-glucanase 3
    Short name:
    EG3
    Gene namesi
    Name:cel-3
    Ordered Locus Names:Fisuc_2230, FSU_2772
    OrganismiFibrobacter succinogenes (strain ATCC 19169 / S85)
    Taxonomic identifieri59374 [NCBI]
    Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter
    ProteomesiUP000000517: Chromosome, UP000001497: Chromosome

    Subcellular locationi

    Membrane Curated; Lipid-anchor PROSITE-ProRule annotation

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323PROSITE-ProRule annotationAdd
    BLAST
    Propeptidei24 – 2652421 PublicationPRO_0000007861Add
    BLAST
    Chaini266 – 658393Endoglucanase 3PRO_0000007862Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi24 – 241N-palmitoyl cysteinePROSITE-ProRule annotation
    Lipidationi24 – 241S-diacylglycerol cysteinePROSITE-ProRule annotation

    Post-translational modificationi

    May be a lipoprotein and may be glycosylated.

    Keywords - PTMi

    Lipoprotein, Palmitate, Zymogen

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    STRINGi59374.Fisuc_2230.

    Structurei

    3D structure databases

    ProteinModelPortaliP14250.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2730.
    OMAiDEANGHF.
    OrthoDBiEOG6NPM7N.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR005087. CBM_fam11.
    IPR008979. Galactose-bd-like.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF03425. CBM_11. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 2 hits.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P14250-1 [UniParc]FASTAAdd to Basket

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    MQLKNFYPKM SVLGIATVMA LTACGDENTQ ALFANNPVPG AENQVPVSSS    50
    DMSPTSSDAV IDPTSSSAAV VDPSTLPAEG PITMPEGLGT LVDDFEDGDN 100
    LSKIGDYWYT YNDNDNGGAS IITTPLNEEE NIIPGRVNNG SNYALQVNYT 150
    LDRGDYEYDP YVGWGVQVAP DEANGHFGGL TYWYKGGAHE VHIEITDVED 200
    YDVHLAKFPA SRTWKQAVVR FKDLVQGGWG KEIPFDAKHI MAISFQAKGN 250
    KSKLVTDSLF IDNIYLQDSS EVEKDQPDME IKDPVIPVVE FTEAEITVTN 300
    PLQEKAMKYL NKGVNFTNWL ENADGKFKSF ELGESDVKIL ADNGFKSLRL 350
    PIDLDLYATN RDAFIAGTDT ELKFDDDTLF LVLDSFVEWT AKYNMSFVID 400
    YHEYDNSYNT TSAKDPNYIK MMAETWKHVA AHYAESPRED LFFELLNEPD 450
    MSDGKVTAAT WTTAAQAMID AIRTVDTKHT ILFGDAQWYS ITLLAKRTPF 500
    TDDNIIYVIH TYEPFAFTHQ GGSWTDYATI HDIPFPYDPA KWSTVSGDFG 550
    VNKSTKSYVK TNIKNYYKTG SKEAILEQIL KAKKWAATNN VPVIINEFGA 600
    LNLRSTAESR LNYLTAMREI CDTLQIPWTH WGYTGNFSVI ENGKLIEGLD 650
    KALGVGSK 658
    Length:658
    Mass (Da):73,424
    Last modified:February 1, 1996 - v2
    Checksum:i1C96E64C3F7109A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29047 Genomic DNA. Translation: AAA24893.1.
    CP001792 Genomic DNA. Translation: ACX75816.1.
    CP002158 Genomic DNA. Translation: ADL25000.1.
    PIRiA33598.
    RefSeqiWP_014546871.1. NC_017448.1.
    YP_003250298.1. NC_013410.1.
    YP_005822586.1. NC_017448.1.

    Genome annotation databases

    EnsemblBacteriaiACX75816; ACX75816; Fisuc_2230.
    ADL25000; ADL25000; FSU_2772.
    GeneIDi12434275.
    8522862.
    KEGGifsc:FSU_2772.
    fsu:Fisuc_2230.
    PATRICi32144235. VBIFibSuc28982_2179.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M29047 Genomic DNA. Translation: AAA24893.1 .
    CP001792 Genomic DNA. Translation: ACX75816.1 .
    CP002158 Genomic DNA. Translation: ADL25000.1 .
    PIRi A33598.
    RefSeqi WP_014546871.1. NC_017448.1.
    YP_003250298.1. NC_013410.1.
    YP_005822586.1. NC_017448.1.

    3D structure databases

    ProteinModelPortali P14250.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 59374.Fisuc_2230.

    Protein family/group databases

    CAZyi GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACX75816 ; ACX75816 ; Fisuc_2230 .
    ADL25000 ; ADL25000 ; FSU_2772 .
    GeneIDi 12434275.
    8522862.
    KEGGi fsc:FSU_2772.
    fsu:Fisuc_2230.
    PATRICi 32144235. VBIFibSuc28982_2179.

    Phylogenomic databases

    eggNOGi COG2730.
    OMAi DEANGHF.
    OrthoDBi EOG6NPM7N.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR005087. CBM_fam11.
    IPR008979. Galactose-bd-like.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF03425. CBM_11. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 2 hits.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    PS51257. PROKAR_LIPOPROTEIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3."
      McGavin M.J., Forsberg C.W., Crosby B., Bell A.W., Dignard D., Thomas D.Y.
      J. Bacteriol. 171:5587-5595(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 266-287.
    2. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
      US DOE Joint Genome Institute
      Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J.
      , Stevenson D.M., Boyum J., Brumm P.I., Mead D.
      Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19169 / S85.
    3. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
      Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
      Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 19169 / S85.

    Entry informationi

    Entry nameiGUN3_FIBSS
    AccessioniPrimary (citable) accession number: P14250
    Secondary accession number(s): C9RK83, D9S6Q5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 100 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3