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P14250 (GUN3_FIBSS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoglucanase 3

EC=3.2.1.4
Alternative name(s):
Cellulase 3
Endo-1,4-beta-glucanase 3
Short name=EG3
Gene names
Name:cel-3
Ordered Locus Names:Fisuc_2230, FSU_2772
OrganismFibrobacter succinogenes (strain ATCC 19169 / S85) [Complete proteome] [HAMAP]
Taxonomic identifier59374 [NCBI]
Taxonomic lineageBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exhibits both endoglucanase and cellobiosidase activities.

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Subunit structure

Monomer.

Subcellular location

Membrane; Lipid-anchor Potential.

Post-translational modification

May be a lipoprotein and may be glycosylated.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM11 (carbohydrate binding type-11) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   Cellular componentMembrane
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMLipoprotein
Palmitate
Zymogen
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Propeptide24 – 265242
PRO_0000007861
Chain266 – 658393Endoglucanase 3
PRO_0000007862

Sites

Active site4481Proton donor By similarity
Active site5971Nucleophile By similarity

Amino acid modifications

Lipidation241N-palmitoyl cysteine Potential
Lipidation241S-diacylglycerol cysteine Potential

Sequences

Sequence LengthMass (Da)Tools
P14250 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 1C96E64C3F7109A6

FASTA65873,424
        10         20         30         40         50         60 
MQLKNFYPKM SVLGIATVMA LTACGDENTQ ALFANNPVPG AENQVPVSSS DMSPTSSDAV 

        70         80         90        100        110        120 
IDPTSSSAAV VDPSTLPAEG PITMPEGLGT LVDDFEDGDN LSKIGDYWYT YNDNDNGGAS 

       130        140        150        160        170        180 
IITTPLNEEE NIIPGRVNNG SNYALQVNYT LDRGDYEYDP YVGWGVQVAP DEANGHFGGL 

       190        200        210        220        230        240 
TYWYKGGAHE VHIEITDVED YDVHLAKFPA SRTWKQAVVR FKDLVQGGWG KEIPFDAKHI 

       250        260        270        280        290        300 
MAISFQAKGN KSKLVTDSLF IDNIYLQDSS EVEKDQPDME IKDPVIPVVE FTEAEITVTN 

       310        320        330        340        350        360 
PLQEKAMKYL NKGVNFTNWL ENADGKFKSF ELGESDVKIL ADNGFKSLRL PIDLDLYATN 

       370        380        390        400        410        420 
RDAFIAGTDT ELKFDDDTLF LVLDSFVEWT AKYNMSFVID YHEYDNSYNT TSAKDPNYIK 

       430        440        450        460        470        480 
MMAETWKHVA AHYAESPRED LFFELLNEPD MSDGKVTAAT WTTAAQAMID AIRTVDTKHT 

       490        500        510        520        530        540 
ILFGDAQWYS ITLLAKRTPF TDDNIIYVIH TYEPFAFTHQ GGSWTDYATI HDIPFPYDPA 

       550        560        570        580        590        600 
KWSTVSGDFG VNKSTKSYVK TNIKNYYKTG SKEAILEQIL KAKKWAATNN VPVIINEFGA 

       610        620        630        640        650 
LNLRSTAESR LNYLTAMREI CDTLQIPWTH WGYTGNFSVI ENGKLIEGLD KALGVGSK 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3."
McGavin M.J., Forsberg C.W., Crosby B., Bell A.W., Dignard D., Thomas D.Y.
J. Bacteriol. 171:5587-5595(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 266-287.
[2]"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
US DOE Joint Genome Institute
Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J. expand/collapse author list , Stevenson D.M., Boyum J., Brumm P.I., Mead D.
Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19169 / S85.
[3]"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 19169 / S85.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29047 Genomic DNA. Translation: AAA24893.1.
CP001792 Genomic DNA. Translation: ACX75816.1.
CP002158 Genomic DNA. Translation: ADL25000.1.
PIRA33598.
RefSeqYP_003250298.1. NC_013410.1.
YP_005822586.1. NC_017448.1.

3D structure databases

ProteinModelPortalP14250.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING59374.Fisuc_2230.

Protein family/group databases

CAZyGH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACX75816; ACX75816; Fisuc_2230.
ADL25000; ADL25000; FSU_2772.
GeneID12434275.
8522862.
KEGGfsc:FSU_2772.
fsu:Fisuc_2230.
PATRIC32144235. VBIFibSuc28982_2179.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2730.
OMADEANGHF.
OrthoDBEOG6NPM7N.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR005087. CBM_fam11.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF03425. CBM_11. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN3_FIBSS
AccessionPrimary (citable) accession number: P14250
Secondary accession number(s): C9RK83, D9S6Q5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: May 14, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries