P14250 (GUN3_FIBSS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase 3
EC=3.2.1.4

Alternative name(s):
Cellulase 3
Endo-1,4-beta-glucanase 3
Short name=EG3

Gene names

Name:	cel-3
Ordered Locus Names:	Fisuc_2230, FSU_2772

Organism

Fibrobacter succinogenes (strain ATCC 19169 / S85) [Complete proteome] [HAMAP]

Taxonomic identifier

59374 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Fibrobacteres/Acidobacteria group › Fibrobacteres › Fibrobacteria › Fibrobacterales › Fibrobacteraceae › Fibrobacter › Fibrobacter succinogenes › Fibrobacter succinogenes subsp. succinogenes

Protein attributes

Sequence length	658 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exhibits both endoglucanase and cellobiosidase activities.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Subunit structure	Monomer.
Subcellular location	Membrane; Lipid-anchor Potential.
Post-translational modification	May be a lipoprotein and may be glycosylated.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM11 (carbohydrate binding type-11) domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Membrane
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate Zymogen
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 265	242			PRO_0000007861
	Chain	266 – 658	393	Endoglucanase 3		PRO_0000007862
Sites
	Active site	448	1	Proton donor By similarity
	Active site	597	1	Nucleophile By similarity
Amino acid modifications
	Lipidation	24	1	N-palmitoyl cysteine Potential
	Lipidation	24	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	P14250 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 1C96E64C3F7109A6	FASTA	658	73,424
	10 20 30 40 50 60 MQLKNFYPKM SVLGIATVMA LTACGDENTQ ALFANNPVPG AENQVPVSSS DMSPTSSDAV 70 80 90 100 110 120 IDPTSSSAAV VDPSTLPAEG PITMPEGLGT LVDDFEDGDN LSKIGDYWYT YNDNDNGGAS 130 140 150 160 170 180 IITTPLNEEE NIIPGRVNNG SNYALQVNYT LDRGDYEYDP YVGWGVQVAP DEANGHFGGL 190 200 210 220 230 240 TYWYKGGAHE VHIEITDVED YDVHLAKFPA SRTWKQAVVR FKDLVQGGWG KEIPFDAKHI 250 260 270 280 290 300 MAISFQAKGN KSKLVTDSLF IDNIYLQDSS EVEKDQPDME IKDPVIPVVE FTEAEITVTN 310 320 330 340 350 360 PLQEKAMKYL NKGVNFTNWL ENADGKFKSF ELGESDVKIL ADNGFKSLRL PIDLDLYATN 370 380 390 400 410 420 RDAFIAGTDT ELKFDDDTLF LVLDSFVEWT AKYNMSFVID YHEYDNSYNT TSAKDPNYIK 430 440 450 460 470 480 MMAETWKHVA AHYAESPRED LFFELLNEPD MSDGKVTAAT WTTAAQAMID AIRTVDTKHT 490 500 510 520 530 540 ILFGDAQWYS ITLLAKRTPF TDDNIIYVIH TYEPFAFTHQ GGSWTDYATI HDIPFPYDPA 550 560 570 580 590 600 KWSTVSGDFG VNKSTKSYVK TNIKNYYKTG SKEAILEQIL KAKKWAATNN VPVIINEFGA 610 620 630 640 650 LNLRSTAESR LNYLTAMREI CDTLQIPWTH WGYTGNFSVI ENGKLIEGLD KALGVGSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3." McGavin M.J., Forsberg C.W., Crosby B., Bell A.W., Dignard D., Thomas D.Y. J. Bacteriol. 171:5587-5595(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 266-287.
[2]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." US DOE Joint Genome Institute Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J. , Stevenson D.M., Boyum J., Brumm P.I., Mead D. Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.
[3]	"Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85." Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A. Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 19169 / S85.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29047 Genomic DNA. Translation: AAA24893.1. CP001792 Genomic DNA. Translation: ACX75816.1. CP002158 Genomic DNA. Translation: ADL25000.1.
PIR	A33598.
RefSeq	YP_003250298.1. NC_013410.1. YP_005822586.1. NC_017448.1.
3D structure databases
ProteinModelPortal	P14250.
ModBase	Search...
Protein-protein interaction databases
STRING	59374.Fisuc_2230.
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACX75816; ACX75816; Fisuc_2230. ADL25000; ADL25000; FSU_2772.
GeneID	12434275. 8522862.
KEGG	fsc:FSU_2772. fsu:Fisuc_2230.
PATRIC	32144235. VBIFibSuc28982_2179.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2730.
OMA	DEANGHF.
ProtClustDB	CLSK2530672.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR005087. CBM_fam11. IPR008979. Galactose-bd-like. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF03425. CBM_11. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN3_FIBSS

Accession

Primary (citable) accession number: P14250
Secondary accession number(s): C9RK83, D9S6Q5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program