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P14250

- GUN3_FIBSS

UniProt

P14250 - GUN3_FIBSS

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Protein

Endoglucanase 3

Gene
cel-3, Fisuc_2230, FSU_2772
Organism
Fibrobacter succinogenes (strain ATCC 19169 / S85)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Exhibits both endoglucanase and cellobiosidase activities.

Catalytic activityi

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei448 – 4481Proton donor By similarity
Active sitei597 – 5971Nucleophile By similarity

GO - Molecular functioni

  1. cellulase activity Source: UniProtKB-EC

GO - Biological processi

  1. cellulose catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation

Protein family/group databases

CAZyiGH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoglucanase 3 (EC:3.2.1.4)
Alternative name(s):
Cellulase 3
Endo-1,4-beta-glucanase 3
Short name:
EG3
Gene namesi
Name:cel-3
Ordered Locus Names:Fisuc_2230, FSU_2772
OrganismiFibrobacter succinogenes (strain ATCC 19169 / S85)
Taxonomic identifieri59374 [NCBI]
Taxonomic lineageiBacteriaFibrobacteresFibrobacteralesFibrobacteraceaeFibrobacter
ProteomesiUP000000517: Chromosome, UP000001497: Chromosome

Subcellular locationi

Membrane; Lipid-anchor Reviewed prediction

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Propeptidei24 – 265242PRO_0000007861Add
BLAST
Chaini266 – 658393Endoglucanase 3PRO_0000007862Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi24 – 241N-palmitoyl cysteine Reviewed prediction
Lipidationi24 – 241S-diacylglycerol cysteine Reviewed prediction

Post-translational modificationi

May be a lipoprotein and may be glycosylated.

Keywords - PTMi

Lipoprotein, Palmitate, Zymogen

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi59374.Fisuc_2230.

Structurei

3D structure databases

ProteinModelPortaliP14250.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG2730.
OMAiDEANGHF.
OrthoDBiEOG6NPM7N.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR005087. CBM_fam11.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF03425. CBM_11. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14250-1 [UniParc]FASTAAdd to Basket

« Hide

MQLKNFYPKM SVLGIATVMA LTACGDENTQ ALFANNPVPG AENQVPVSSS    50
DMSPTSSDAV IDPTSSSAAV VDPSTLPAEG PITMPEGLGT LVDDFEDGDN 100
LSKIGDYWYT YNDNDNGGAS IITTPLNEEE NIIPGRVNNG SNYALQVNYT 150
LDRGDYEYDP YVGWGVQVAP DEANGHFGGL TYWYKGGAHE VHIEITDVED 200
YDVHLAKFPA SRTWKQAVVR FKDLVQGGWG KEIPFDAKHI MAISFQAKGN 250
KSKLVTDSLF IDNIYLQDSS EVEKDQPDME IKDPVIPVVE FTEAEITVTN 300
PLQEKAMKYL NKGVNFTNWL ENADGKFKSF ELGESDVKIL ADNGFKSLRL 350
PIDLDLYATN RDAFIAGTDT ELKFDDDTLF LVLDSFVEWT AKYNMSFVID 400
YHEYDNSYNT TSAKDPNYIK MMAETWKHVA AHYAESPRED LFFELLNEPD 450
MSDGKVTAAT WTTAAQAMID AIRTVDTKHT ILFGDAQWYS ITLLAKRTPF 500
TDDNIIYVIH TYEPFAFTHQ GGSWTDYATI HDIPFPYDPA KWSTVSGDFG 550
VNKSTKSYVK TNIKNYYKTG SKEAILEQIL KAKKWAATNN VPVIINEFGA 600
LNLRSTAESR LNYLTAMREI CDTLQIPWTH WGYTGNFSVI ENGKLIEGLD 650
KALGVGSK 658
Length:658
Mass (Da):73,424
Last modified:February 1, 1996 - v2
Checksum:i1C96E64C3F7109A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29047 Genomic DNA. Translation: AAA24893.1.
CP001792 Genomic DNA. Translation: ACX75816.1.
CP002158 Genomic DNA. Translation: ADL25000.1.
PIRiA33598.
RefSeqiWP_014546871.1. NC_017448.1.
YP_003250298.1. NC_013410.1.
YP_005822586.1. NC_017448.1.

Genome annotation databases

EnsemblBacteriaiACX75816; ACX75816; Fisuc_2230.
ADL25000; ADL25000; FSU_2772.
GeneIDi12434275.
8522862.
KEGGifsc:FSU_2772.
fsu:Fisuc_2230.
PATRICi32144235. VBIFibSuc28982_2179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M29047 Genomic DNA. Translation: AAA24893.1 .
CP001792 Genomic DNA. Translation: ACX75816.1 .
CP002158 Genomic DNA. Translation: ADL25000.1 .
PIRi A33598.
RefSeqi WP_014546871.1. NC_017448.1.
YP_003250298.1. NC_013410.1.
YP_005822586.1. NC_017448.1.

3D structure databases

ProteinModelPortali P14250.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 59374.Fisuc_2230.

Protein family/group databases

CAZyi GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACX75816 ; ACX75816 ; Fisuc_2230 .
ADL25000 ; ADL25000 ; FSU_2772 .
GeneIDi 12434275.
8522862.
KEGGi fsc:FSU_2772.
fsu:Fisuc_2230.
PATRICi 32144235. VBIFibSuc28982_2179.

Phylogenomic databases

eggNOGi COG2730.
OMAi DEANGHF.
OrthoDBi EOG6NPM7N.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR005087. CBM_fam11.
IPR008979. Galactose-bd-like.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF03425. CBM_11. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 2 hits.
PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
PS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3."
    McGavin M.J., Forsberg C.W., Crosby B., Bell A.W., Dignard D., Thomas D.Y.
    J. Bacteriol. 171:5587-5595(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 266-287.
  2. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
    US DOE Joint Genome Institute
    Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D., Goodwin L., Pitluck S., Chertkov O., Detter J.C., Han C., Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Weimer P.J.
    , Stevenson D.M., Boyum J., Brumm P.I., Mead D.
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19169 / S85.
  3. "Complete sequence of Fibrobacter succinogenes subsp. succinogenes S85."
    Durkin A.S., Nelson K.E., Morrison M., Forsberg C.W., Wilson D.B., Russell J.B., Cann I.K.O., Mackie R.I., White B.A.
    Submitted (AUG-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 19169 / S85.

Entry informationi

Entry nameiGUN3_FIBSS
AccessioniPrimary (citable) accession number: P14250
Secondary accession number(s): C9RK83, D9S6Q5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: September 3, 2014
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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