Reviewed,
UniProtKB/Swiss-Prot P14250 (GUN3_FIBSU)
Last modified
October 13, 2009.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Endoglucanase 3 EC=3.2.1.4 Alternative name(s): Endo-1,4-beta-glucanase 3 Short name=EG3 Cellulase 3 | ||
| Gene names |
| ||
| Organism | Fibrobacter succinogenes (Bacteroides succinogenes) | ||
| Taxonomic identifier | 833 [NCBI] | ||
| Taxonomic lineage | Bacteria › Fibrobacteres › Fibrobacterales › Fibrobacteraceae › Fibrobacter |
Protein attributes
| Sequence length | 658 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Exhibits both endoglucanase and cellobiosidase activities. |
| Catalytic activity | Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. |
| Subunit structure | Monomer. |
| Subcellular location | Membrane; Lipid-anchor Potential. |
| Post-translational modification | May be a lipoprotein and may be glycosylated. |
| Sequence similarities | Belongs to the glycosyl hydrolase 5 (cellulase A) family. Contains 1 CBM11 (carbohydrate binding type-11) domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism Cellulose degradation Polysaccharide degradation |
| Cellular component | Membrane |
| Domain | Signal |
| Molecular function | Glycosidase Hydrolase |
| PTM | Lipoprotein Palmitate Zymogen |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | cation binding Inferred from electronic annotation. Source: InterPro cellulase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 23 | 23 | Potential | ||||||
| Propeptide | 24 – 265 | 242 | PRO_0000007861 | ||||||
| Chain | 266 – 658 | 393 | Endoglucanase 3 | PRO_0000007862 | |||||
Sites | |||||||||
| Active site | 448 | 1 | Proton donor By similarity | ||||||
| Active site | 597 | 1 | Nucleophile By similarity | ||||||
Amino acid modifications | |||||||||
| Lipidation | 24 | 1 | N-palmitoyl cysteine Potential | ||||||
| Lipidation | 24 | 1 | S-diacylglycerol cysteine Potential | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Structure of the cel-3 gene from Fibrobacter succinogenes S85 and characteristics of the encoded gene product, endoglucanase 3." McGavin M.J., Forsberg C.W., Crosby B., Bell A.W., Dignard D., Thomas D.Y. J. Bacteriol. 171:5587-5595(1989) [PubMed: 2676979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 266-287. Strain: S85 / ATCC 19169. |
Cross-references
Sequence databases | |
|---|---|
| M29047 Genomic DNA. Translation: AAA24893.1. | |
| PIR | A33598. |
3D structure databases | |
| HSSP | HSSP built from PDB template 1CEO based on UniProtKB P07985. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH5. Glycoside Hydrolase Family 5. |
Enzyme and pathway databases | |
| BRENDA | 3.2.1.4. 15235. |
Family and domain databases | |
| InterPro | IPR005087. Carb-bd_dom_fam11. IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view] |
| Gene3D | G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. |
| Pfam | PF03425. CBM_11. 1 hit. PF00150. Cellulase. 1 hit. [Graphical view] |
| PROSITE | PS00659. GLYCOSYL_HYDROL_F5. 1 hit. PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | GUN3_FIBSU | ||||||||
| Accession | Primary (citable) accession number: P14250 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

Clusters with


