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P14246 (GTR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 2, facilitated glucose transporter member 2
Alternative name(s):
Glucose transporter type 2, liver
Short name=GLUT-2
Gene names
Name:Slc2a2
Synonyms:Glut-2, Glut2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length523 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na+/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

In embryo, expressed in endoderm layer of yolk sac and liver primordium. Ref.7

Post-translational modification

N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells. Ref.8

Sequence similarities

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily. [View classification]

Ontologies

Keywords
   Biological processSugar transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processendocrine pancreas development

Traceable author statement. Source: Reactome

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionD-glucose transmembrane transporter activity

Inferred from direct assay. Source: UniProtKB

dehydroascorbic acid transporter activity

Inferred from direct assay. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 523523Solute carrier family 2, facilitated glucose transporter member 2
PRO_0000050347

Regions

Topological domain1 – 1010Cytoplasmic Potential
Transmembrane11 – 3121Helical; Name=1; Potential
Topological domain32 – 9766Extracellular Potential
Transmembrane98 – 11821Helical; Name=2; Potential
Topological domain119 – 1268Cytoplasmic Potential
Transmembrane127 – 14721Helical; Name=3; Potential
Topological domain148 – 15710Extracellular Potential
Transmembrane158 – 17821Helical; Name=4; Potential
Topological domain179 – 1868Cytoplasmic Potential
Transmembrane187 – 20721Helical; Name=5; Potential
Topological domain208 – 2169Extracellular Potential
Transmembrane217 – 23721Helical; Name=6; Potential
Topological domain238 – 30265Cytoplasmic Potential
Transmembrane303 – 32321Helical; Name=7; Potential
Topological domain324 – 33714Extracellular Potential
Transmembrane338 – 35821Helical; Name=8; Potential
Topological domain359 – 3679Cytoplasmic Potential
Transmembrane368 – 38821Helical; Name=9; Potential
Topological domain389 – 40113Extracellular Potential
Transmembrane402 – 42221Helical; Name=10; Potential
Topological domain423 – 43210Cytoplasmic Potential
Transmembrane433 – 45321Helical; Name=11; Potential
Topological domain454 – 4607Extracellular Potential
Transmembrane461 – 48121Helical; Name=12; Potential
Topological domain482 – 52342Cytoplasmic Potential

Amino acid modifications

Modified residue5201Phosphoserine Ref.9
Glycosylation621N-linked (GlcNAc...) Probable

Experimental info

Sequence conflict1061G → D in CAA33719. Ref.2
Sequence conflict1811T → N in CAA34855. Ref.1
Sequence conflict3271S → T in CAA33719. Ref.2
Sequence conflict3511F → L in CAA34855. Ref.1
Sequence conflict4321P → S in CAA34855. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14246 [UniParc].

Last modified November 16, 2001. Version 3.
Checksum: F12B5376CBDB5F63

FASTA52357,107
        10         20         30         40         50         60 
MSEDKITGTL AFTVFTAVLS SFQFGYDIGV INAPQEVIIS HYRHVLGVPL DDRKAAINYD 

        70         80         90        100        110        120 
VNGTDTPLTV TPAYTTPAPW DEEETEGSAH IVTMLWSLSV SSFAVGGMVA SFFGGWLGDK 

       130        140        150        160        170        180 
LGRIKAMLAA NSLSLTGALL MGCSKFGPAH ALIIAGRSVS GLYCGLISGL VPMYIGEIAP 

       190        200        210        220        230        240 
TTLRGALGTL HQLALVTGIL ISQIAGLSFI LGNQDHWHIL LGLSAVPALL QCLLLLFCPE 

       250        260        270        280        290        300 
SPRYLYIKLE EEVRAKKSLK RLRGTEDVTK DINEMKKEKE EASTEQKVSV IQLFTDANYR 

       310        320        330        340        350        360 
QPILVALMLH MAQQFSGING IFYYSTSIFQ TAGISQPVYA TIGVGAINMI FTAVSVLLVE 

       370        380        390        400        410        420 
KAGRRTLFLT GMIGMFFCTI FMSVGLVLLD KFAWMSYVSM TAIFLFVSFF EIGPGPIPWF 

       430        440        450        460        470        480 
MVAEFFSQGP RPTALALAAF SNWVCNFVIA LCFQYIADFL GPYVFFLFAG VVLVFTLFTF 

       490        500        510        520 
FKVPETKGKS FEEIAAEFRK KSGSAPPRKA AVQMEFLASS ESV

« Hide

References

« Hide 'large scale' references
[1]"Sequence of the mouse liver glucose transporter."
Suzue K., Lodish H.F., Thorens B.
Nucleic Acids Res. 17:10099-10099(1989) [PubMed: 2602116] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[2]"The nucleotide sequence of cDNA for a mouse liver-type glucose transporter protein."
Asano T., Shibasaki Y., Lin J.L., Akanuma Y., Takaku F., Oka Y.
Nucleic Acids Res. 17:6386-6386(1989) [PubMed: 2771649] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
Tissue: Liver.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney and Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[5]"Characterization of the murine high Km glucose transporter GLUT2 gene and its transcriptional regulation by glucose in a differentiated insulin-secreting cell line."
Waeber G., Thompson N., Haefliger J.-A., Nicod P.
J. Biol. Chem. 269:26912-26919(1994) [PubMed: 7929431] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
[6]"Glucose transporter gene expression in early mouse embryos."
Hogan A., Heyner S., Charron M.J., Copeland N.G., Gilbert D.J., Jenkins N.A., Thorens B., Schultz G.A.
Development 113:363-372(1991) [PubMed: 1765007] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 384-496.
[7]"Differential screening of a PCR-generated mouse embryo cDNA library: glucose transporters are differentially expressed in early postimplantation mouse embryos."
Smith D.E., Gridley T.
Development 116:555-561(1992) [PubMed: 1289053] [Abstract]
Cited for: TISSUE SPECIFICITY.
Strain: C57BL/6.
[8]"Dietary and genetic control of glucose transporter 2 glycosylation promotes insulin secretion in suppressing diabetes."
Ohtsubo K., Takamatsu S., Minowa M.T., Yoshida A., Takeuchi M., Marth J.D.
Cell 123:1307-1321(2005) [PubMed: 16377570] [Abstract]
Cited for: GLYCOSYLATION AT ASN-62.
Strain: 129/SvJ.
[9]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-520, MASS SPECTROMETRY.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X16986 mRNA. Translation: CAA34855.1.
X15684 mRNA. Translation: CAA33719.1.
S77926 mRNA. Translation: AAB20847.1.
AK005068 mRNA. Translation: BAB23792.1.
AK165430 mRNA. Translation: BAE38184.1.
BC034675 mRNA. Translation: AAH34675.1.
X78722 Genomic DNA. Translation: CAA55368.1.
IPIIPI00311809.
PIRS06920.
RefSeqNP_112474.2. NM_031197.2.
UniGeneMm.18443.

3D structure databases

ProteinModelPortalP14246.
ModBaseSearch...

Protein-protein interaction databases

STRINGP14246.

PTM databases

PhosphoSiteP14246.

Proteomic databases

PRIDEP14246.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029240; ENSMUSP00000029240; ENSMUSG00000027690.
GeneID20526.
KEGGmmu:20526.
UCSCuc008oua.2. mouse.

Organism-specific databases

CTD6514.
MGIMGI:1095438. Slc2a2.

Phylogenomic databases

eggNOGroNOG12108.
GeneTreeENSGT00600000084007.
HOGENOMHBG744444.
HOVERGENHBG014816.
InParanoidP14246.
OrthoDBEOG4K6G40.
PhylomeDBP14246.

Enzyme and pathway databases

ReactomeREACT_115492. Developmental Biology.
REACT_13641. Regulation of Beta-Cell Development.

Gene expression databases

ArrayExpressP14246.
BgeeP14246.
GenevestigatorP14246.
GermOnlineENSMUSG00000027690. Mus musculus.

Family and domain databases

InterProIPR002440. Glc_transpt_2.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
KOK07593.
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSPR01191. GLUCTRSPORT2.
PR00171. SUGRTRNSPORT.
SUPFAMSSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMsTIGR00879. SP. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio298767.
SOURCESearch...

Entry information

Entry nameGTR2_MOUSE
AccessionPrimary (citable) accession number: P14246
Secondary accession number(s): Q3TNA5, Q9DBA7
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 16, 2001
Last modified: November 16, 2011
This is version 123 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents