Reviewed, UniProtKB/Swiss-Prot P14244 (MTMV_MICVA)

Names and origin

Protein names

Recommended name:
    Modification methylase MvaI
      Short name=M.MvaI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase MvaI

Gene names

Name:

mvaIM

Organism

Micrococcus varians

Taxonomic identifier

1272 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Micrococcaceae › Kocuria

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCWGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the MvaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Modification methylase MvaI		PRO_0000087929

Sequences

	Sequence		Length	Mass (Da)
	P14244-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: B0330A9DF037E93A	FASTA	454	53,072
	10 20 30 40 50 60 MEYLNDKDQH LIDKLSKKIN DNNQYLGFLN TNTKELTHRY HIYPAMMIPQ LAKEFIELTQ 70 80 90 100 110 120 QVKPEIKKLY DPFMGSGTSL VEGLAHGLEV YGTDINPLSQ MMSKAKTTPI EPSKLSRAIS 130 140 150 160 170 180 DLEYSIREMT ILYHEGNYKI SNLPDFDRID FWFKEEVIIS LQLIKNCINE FIEDDLKTFF 190 200 210 220 230 240 MAAFSETVRH VSNTRNNEFK LYRMAPEKLE IWNPNVTEEF LKRVYRNELG NMDFYRQLEN 250 260 270 280 290 300 VGNYSPKTII NKQSNIKLPE EFKDEMFDIV VTSPPYGDSK TTVAYGQFSR LSAQWLDLKI 310 320 330 340 350 360 DDETKINQLD NVMLGGKTDK NIIVNDVLEY LNSPTSKSVF NLISHKDEKR ALEVLQFYVD 370 380 390 400 410 420 LDKSIKETTR VMKPESYQFW VVANRTVKMI SIPTDIIISE LFKKYNVHHL YSFYRKIPNK 430 440 450 RMPSKNSPTN KIGNHSVTMT SEIILMLKNY INKS

References

[1]

"Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases." Klimasauskas S., Timinskas A., Menkevicius S., Butkiene D., Butkus V., Janulaitis A. Nucleic Acids Res. 17:9823-9832(1989) [PubMed: 2690010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL19.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16985 Genomic DNA. Translation: CAA34854.1.
PIR	S07539.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	3450. M.MvaI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 1281.
Family and domain databases
InterPro	IPR017985. MeTrfase_CN4_CS. [Graphical view]
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTMV_MICVA

Accession

Primary (citable) accession number: P14244

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)