Reviewed, UniProtKB/Swiss-Prot P14243 (MTC9_CITFR)

Names and origin

Protein names

Recommended name:
    Modification methylase Cfr9I
      Short name=M.Cfr9I
    EC=2.1.1.113
Alternative name(s):
    N(4)- cytosine-specific methyltransferase Cfr9I

Gene names

Name:

cfr9IM

Organism

Citrobacter freundii

Taxonomic identifier

546 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Citrobacter

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the Cfr9I endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Modification methylase Cfr9I		PRO_0000087927

Sequences

	Sequence		Length	Mass (Da)
	P14243-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 972B291C551CBF2F	FASTA	300	33,781
	10 20 30 40 50 60 MPSKKSSSPL SVEKLHRSEP LELNGATLFE GDALSVLRRL PSGSVRCIVT SPPYWGLRDY 70 80 90 100 110 120 GIDEQIGLES SMTQFLNRLV TIFSEAKRVL TDDGTLWVNI GDGYTSGNRG YRAPDKKNPA 130 140 150 160 170 180 RAMAVRPDTP EGLKPKDLIG IPWRLAFALQ EDGWYLRSDI VWNKPNAMPE SVKDRPTRSH 190 200 210 220 230 240 EFLFMLTKSE KYYYDWEAVR EEKDSGGFRN RRTVWNVNTK PFAGAHFATF PTELIRPCIL 250 260 270 280 290 300 ASTKPGDYVL DPFFGSGTVG VVCQQEDRQY VGIELNPEYV DIAVNRLQGE DTNVIRIAAA

References

[1]	"Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases." Klimasauskas S., Timinskas A., Menkevicius S., Butkiene D., Butkus V., Janulaitis A. Nucleic Acids Res. 17:9823-9832(1989) [PubMed: 2690010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RFL9.
[2]	"Cloning and analysis of translational control for genes encoding the Cfr9I restriction-modification system." Lubys A., Menkevicius S., Timinskas A., Butkus V., Janulaitis A. Gene 141:85-89(1994) [PubMed: 8163180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-300. Strain: RFL9.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X17022 Genomic DNA. Translation: CAA34887.1. X74517 Genomic DNA. Translation: CAA52629.1.
PIR	S07540.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3346. M.Cfr9I.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC9_CITFR

Accession

Primary (citable) accession number: P14243

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)