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Protein

DNA mismatch repair protein PMS1

Gene

PMS1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA mismatch repair (MMR), correcting base-base mismatches and insertion-deletion loops (IDLs) resulting from DNA replication, DNA damage or from recombination events between non-identical sequences during meiosis. Component of the MutLalpha heterodimer that forms a ternary complex with the MutS heterodimers, which initially recognize the DNA mismatches. This complex is thought to be responsible for directing the downsteam MMR events, including strand discrimination, excision, and resynthesis. Plays a major role in maintaining the genetic stability of simple sequence repeats and in the repair of heteroduplex sites present in meiotic recombination intermediates.3 Publications

Kineticsi

  1. KM=1.5 mM for ATP1 Publication

    GO - Molecular functioni

    • ATPase activity Source: SGD
    • ATP binding Source: SGD
    • mismatched DNA binding Source: InterPro

    GO - Biological processi

    • meiotic mismatch repair Source: SGD
    • mismatch repair Source: SGD
    Complete GO annotation...

    Keywords - Biological processi

    DNA damage, DNA repair

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33111-MONOMER.
    ReactomeiR-SCE-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA mismatch repair protein PMS1
    Alternative name(s):
    Postmeiotic segregation protein 1
    Gene namesi
    Name:PMS1
    Ordered Locus Names:YNL082W
    ORF Names:N2317
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XIV

    Organism-specific databases

    EuPathDBiFungiDB:YNL082W.
    SGDiS000005026. PMS1.

    Subcellular locationi

    GO - Cellular componenti

    • MutLalpha complex Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi30E → A: Reduces ATPase activity by 62%. Displays 60-fold increase in spontaneous mutation accumulation. 2 Publications1
    Mutagenesisi34N → A: Reduces ATPase activity by 84%. Displays 11000-fold increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi95F → A: Displays an increase in spontaneous mutation accumulation. Does not impair heterodimer formation. 1 Publication1
    Mutagenesisi97G → A: Displays an increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi297K → E: Displays a 60-fold increase in spontaneous mutation accumulation. 1 Publication1
    Mutagenesisi851G → E: Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation. 1 Publication1
    Mutagenesisi857H → R: Confers a strong defect in the repair of primer strand-specific 1-bp loops during DNA replication, but not during meoitic recombination. Does not impair heterodimer formation. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002455711 – 873DNA mismatch repair protein PMS1Add BLAST873

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei393PhosphoserineCombined sources1
    Modified residuei566PhosphoserineCombined sources1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP14242.
    PRIDEiP14242.

    PTM databases

    iPTMnetiP14242.

    Interactioni

    Subunit structurei

    Heterodimer of MLH1 and PMS1, called MutLalpha, which is the major MMR MutL activity correcting base-base mismatches as well as IDLs. The heterodimer binds double strand DNA independently of a mismatch with positive cooperativity and has more than one DNA binding site. Forms a ternary complex with either the MSH2-MSH6 (MutSalpha) or the MSH2-MSH3 heterodimer (MutSbeta), which recognize and bind to mismatch DNA. Ternary complex formation is promoted by ATP binding.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MLH1P389207EBI-13561,EBI-11003

    Protein-protein interaction databases

    BioGridi35741. 53 interactors.
    DIPiDIP-2416N.
    IntActiP14242. 5 interactors.
    MINTiMINT-625253.

    Structurei

    Secondary structure

    1873
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi13 – 20Combined sources8
    Helixi24 – 37Combined sources14
    Beta strandi41 – 48Combined sources8
    Turni49 – 52Combined sources4
    Beta strandi53 – 59Combined sources7
    Helixi66 – 68Combined sources3
    Turni69 – 73Combined sources5
    Beta strandi92 – 95Combined sources4
    Helixi98 – 105Combined sources8
    Beta strandi106 – 116Combined sources11
    Beta strandi120 – 125Combined sources6
    Beta strandi131 – 137Combined sources7
    Beta strandi140 – 149Combined sources10
    Turni150 – 153Combined sources4
    Helixi155 – 162Combined sources8
    Helixi165 – 182Combined sources18
    Beta strandi187 – 193Combined sources7
    Beta strandi199 – 204Combined sources6
    Helixi211 – 219Combined sources9
    Turni221 – 226Combined sources6
    Beta strandi227 – 235Combined sources9
    Helixi237 – 239Combined sources3
    Beta strandi259 – 267Combined sources9
    Beta strandi274 – 285Combined sources12
    Beta strandi288 – 290Combined sources3
    Helixi293 – 304Combined sources12
    Beta strandi314 – 319Combined sources6
    Helixi322 – 324Combined sources3
    Beta strandi330 – 332Combined sources3
    Helixi341 – 361Combined sources21
    Helixi650 – 652Combined sources3
    Helixi653 – 665Combined sources13
    Helixi668 – 672Combined sources5
    Beta strandi675 – 680Combined sources6
    Turni681 – 683Combined sources3
    Beta strandi684 – 690Combined sources7
    Beta strandi695 – 701Combined sources7
    Helixi702 – 717Combined sources16
    Beta strandi722 – 731Combined sources10
    Helixi739 – 742Combined sources4
    Helixi747 – 750Combined sources4
    Beta strandi767 – 773Combined sources7
    Helixi782 – 794Combined sources13
    Helixi806 – 819Combined sources14
    Helixi829 – 838Combined sources10
    Helixi839 – 841Combined sources3
    Beta strandi842 – 844Combined sources3
    Beta strandi853 – 860Combined sources8
    Helixi869 – 871Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3H4LX-ray2.50A/B1-365[»]
    4E4WX-ray2.50B635-873[»]
    4FMNX-ray2.69B635-873[»]
    4FMOX-ray3.04B635-873[»]
    ProteinModelPortaliP14242.
    SMRiP14242.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14242.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni1 – 357DNA- and ATP-bindingAdd BLAST357
    Regioni661 – 873Interaction with MLH1Add BLAST213

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00530000063289.
    InParanoidiP14242.
    KOiK10858.
    OMAiKCCNEVY.
    OrthoDBiEOG092C22BC.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR013507. DNA_mismatch_repair_C.
    IPR014762. DNA_mismatch_repair_CS.
    IPR002099. DNA_mismatch_repair_fam.
    IPR003594. HATPase_C.
    IPR014790. MutL_C.
    IPR028831. Pms1/Pms2/PMS2L.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10073:SF12. PTHR10073:SF12. 2 hits.
    PfamiPF01119. DNA_mis_repair. 1 hit.
    PF08676. MutL_C. 1 hit.
    [Graphical view]
    SMARTiSM01340. DNA_mis_repair. 1 hit.
    SM00853. MutL_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 2 hits.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR00585. mutl. 1 hit.
    PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14242-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTQIHQINDI DVHRITSGQV ITDLTTAVKE LVDNSIDANA NQIEIIFKDY
    60 70 80 90 100
    GLESIECSDN GDGIDPSNYE FLALKHYTSK IAKFQDVAKV QTLGFRGEAL
    110 120 130 140 150
    SSLCGIAKLS VITTTSPPKA DKLEYDMVGH ITSKTTTSRN KGTTVLVSQL
    160 170 180 190 200
    FHNLPVRQKE FSKTFKRQFT KCLTVIQGYA IINAAIKFSV WNITPKGKKN
    210 220 230 240 250
    LILSTMRNSS MRKNISSVFG AGGMRGLEEV DLVLDLNPFK NRMLGKYTDD
    260 270 280 290 300
    PDFLDLDYKI RVKGYISQNS FGCGRNSKDR QFIYVNKRPV EYSTLLKCCN
    310 320 330 340 350
    EVYKTFNNVQ FPAVFLNLEL PMSLIDVNVT PDKRVILLHN ERAVIDIFKT
    360 370 380 390 400
    TLSDYYNRQE LALPKRMCSQ SEQQAQKRLK TEVFDDRSTT HESDNENYHT
    410 420 430 440 450
    ARSESNQSNH AHFNSTTGVI DKSNGTELTS VMDGNYTNVT DVIGSECEVS
    460 470 480 490 500
    VDSSVVLDEG NSSTPTKKLP SIKTDSQNLS DLNLNNFSNP EFQNITSPDK
    510 520 530 540 550
    ARSLEKVVEE PVYFDIDGEK FQEKAVLSQA DGLVFVDNEC HEHTNDCCHQ
    560 570 580 590 600
    ERRGSTDTEQ DDEADSIYAE IEPVEINVRT PLKNSRKSIS KDNYRSLSDG
    610 620 630 640 650
    LTHRKFEDEI LEYNLSTKNF KEISKNGKQM SSIISKRKSE AQENIIKNKD
    660 670 680 690 700
    ELEDFEQGEK YLTLTVSKND FKKMEVVGQF NLGFIIVTRK VDNKYDLFIV
    710 720 730 740 750
    DQHASDEKYN FETLQAVTVF KSQKLIIPQP VELSVIDELV VLDNLPVFEK
    760 770 780 790 800
    NGFKLKIDEE EEFGSRVKLL SLPTSKQTLF DLGDFNELIH LIKEDGGLRR
    810 820 830 840 850
    DNIRCSKIRS MFAMRACRSS IMIGKPLNKK TMTRVVHNLS ELDKPWNCPH
    860 870
    GRPTMRHLME LRDWSSFSKD YEI
    Length:873
    Mass (Da):99,355
    Last modified:July 11, 2006 - v3
    Checksum:i2E0FFBC59B718854
    GO

    Sequence cautioni

    The sequence AAA34885 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00521 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00533 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00545 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00551 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00563 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence AAM00569 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA60176 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA61428 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA95956 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence CAA95957 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti695Y → S in AAA34885 (PubMed:2676974).Curated1
    Sequence conflicti861L → I in AAA34885 (PubMed:2676974).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural varianti41N → S in strain: SK1 and YJM421. 2 Publications1
    Natural varianti112I → T in strain: SK1 and YJM421. 2 Publications1
    Natural varianti384F → V in strain: SK1, YJM320, YJM339 and YJM421. 2 Publications1
    Natural varianti392E → V in strain: YJM320. 1 Publication1
    Natural varianti400T → S in strain: SK1, YJM320, YJM339 and YJM421. 2 Publications1
    Natural varianti401A → S in strain: YJM320 and YJM421. 1 Publication1
    Natural varianti416T → TCEGT in strain: SK1, YJM320, YJM339 and YJM421. 1
    Natural varianti458D → Y in strain: EAY1068. 1 Publication1
    Natural varianti475D → N in strain: YJM339. 1 Publication1
    Natural varianti513Y → F in strain: SK1. 1 Publication1
    Natural varianti564A → V in strain: YJM320. 1 Publication1
    Natural varianti768K → R in strain: YJM320. 1 Publication1
    Natural varianti818R → K in strain: SK1 and YJM320; forms a non-functional heterodimer with MHL1 from strain S288c, resulting in an accumulation of mutations in spore progeny of crosses between these strains. 2 Publications1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M29688 Genomic DNA. Translation: AAA34885.1. Different initiation.
    AF458969 Genomic DNA. Translation: AAM00521.1. Different initiation.
    AF458971 Genomic DNA. Translation: AAM00533.1. Different initiation.
    AF458973 Genomic DNA. Translation: AAM00545.1. Different initiation.
    AF458974 Genomic DNA. Translation: AAM00551.1. Different initiation.
    AF458976 Genomic DNA. Translation: AAM00563.1. Different initiation.
    AF458977 Genomic DNA. Translation: AAM00569.1. Different initiation.
    DQ115393 Genomic DNA. Translation: AAZ22526.1.
    DQ356628 Genomic DNA. Translation: ABC86932.1.
    DQ356629 Genomic DNA. Translation: ABC86933.1.
    DQ356630 Genomic DNA. Translation: ABC86934.1.
    DQ356631 Genomic DNA. Translation: ABC86935.1.
    DQ356632 Genomic DNA. Translation: ABC86936.1.
    X86470 Genomic DNA. Translation: CAA60176.1. Different initiation.
    Z71357 Genomic DNA. Translation: CAA95956.1. Different initiation.
    Z71358 Genomic DNA. Translation: CAA95957.1. Different initiation.
    X89016 Genomic DNA. Translation: CAA61428.1. Different initiation.
    BK006947 Genomic DNA. Translation: DAA10463.1.
    PIRiS53896.
    RefSeqiNP_014317.4. NM_001182920.3.

    Genome annotation databases

    EnsemblFungiiYNL082W; YNL082W; YNL082W.
    GeneIDi855642.
    KEGGisce:YNL082W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M29688 Genomic DNA. Translation: AAA34885.1. Different initiation.
    AF458969 Genomic DNA. Translation: AAM00521.1. Different initiation.
    AF458971 Genomic DNA. Translation: AAM00533.1. Different initiation.
    AF458973 Genomic DNA. Translation: AAM00545.1. Different initiation.
    AF458974 Genomic DNA. Translation: AAM00551.1. Different initiation.
    AF458976 Genomic DNA. Translation: AAM00563.1. Different initiation.
    AF458977 Genomic DNA. Translation: AAM00569.1. Different initiation.
    DQ115393 Genomic DNA. Translation: AAZ22526.1.
    DQ356628 Genomic DNA. Translation: ABC86932.1.
    DQ356629 Genomic DNA. Translation: ABC86933.1.
    DQ356630 Genomic DNA. Translation: ABC86934.1.
    DQ356631 Genomic DNA. Translation: ABC86935.1.
    DQ356632 Genomic DNA. Translation: ABC86936.1.
    X86470 Genomic DNA. Translation: CAA60176.1. Different initiation.
    Z71357 Genomic DNA. Translation: CAA95956.1. Different initiation.
    Z71358 Genomic DNA. Translation: CAA95957.1. Different initiation.
    X89016 Genomic DNA. Translation: CAA61428.1. Different initiation.
    BK006947 Genomic DNA. Translation: DAA10463.1.
    PIRiS53896.
    RefSeqiNP_014317.4. NM_001182920.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3H4LX-ray2.50A/B1-365[»]
    4E4WX-ray2.50B635-873[»]
    4FMNX-ray2.69B635-873[»]
    4FMOX-ray3.04B635-873[»]
    ProteinModelPortaliP14242.
    SMRiP14242.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi35741. 53 interactors.
    DIPiDIP-2416N.
    IntActiP14242. 5 interactors.
    MINTiMINT-625253.

    PTM databases

    iPTMnetiP14242.

    Proteomic databases

    MaxQBiP14242.
    PRIDEiP14242.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYNL082W; YNL082W; YNL082W.
    GeneIDi855642.
    KEGGisce:YNL082W.

    Organism-specific databases

    EuPathDBiFungiDB:YNL082W.
    SGDiS000005026. PMS1.

    Phylogenomic databases

    GeneTreeiENSGT00530000063289.
    InParanoidiP14242.
    KOiK10858.
    OMAiKCCNEVY.
    OrthoDBiEOG092C22BC.

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33111-MONOMER.
    ReactomeiR-SCE-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha).

    Miscellaneous databases

    EvolutionaryTraceiP14242.
    PROiP14242.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    InterProiIPR013507. DNA_mismatch_repair_C.
    IPR014762. DNA_mismatch_repair_CS.
    IPR002099. DNA_mismatch_repair_fam.
    IPR003594. HATPase_C.
    IPR014790. MutL_C.
    IPR028831. Pms1/Pms2/PMS2L.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10073:SF12. PTHR10073:SF12. 2 hits.
    PfamiPF01119. DNA_mis_repair. 1 hit.
    PF08676. MutL_C. 1 hit.
    [Graphical view]
    SMARTiSM01340. DNA_mis_repair. 1 hit.
    SM00853. MutL_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 2 hits.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR00585. mutl. 1 hit.
    PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPMS1_YEAST
    AccessioniPrimary (citable) accession number: P14242
    Secondary accession number(s): D6W197
    , Q2I044, Q2I045, Q45TY4, Q8TG48, Q8TG50, Q8TG54, Q8TG57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: July 11, 2006
    Last modified: November 2, 2016
    This is version 170 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 521 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.