P14241 (L_LYCVW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L
EC=2.7.7.48

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Gene names

Name:

L

Organism

Lymphocytic choriomeningitis virus (strain WE) (LCMV)

Taxonomic identifier

11627 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Arenaviridae › Arenavirus › Old world arenaviruses

Virus host

Homo sapiens (Human) [TaxID: 9606]
Mesocricetus auratus (Golden hamster) [TaxID: 10036]
Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	363 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNA are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N By similarity. Interacts with protein Z; this interaction inhibits viral transcription and replication By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Sequence similarities	Belongs to the arenaviridae RNA polymerase family.

Ontologies

Keywords
Biological process	RNA replication
Cellular component	Host cytoplasm Virion
Ligand	Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular component	host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›363	›363	RNA-directed RNA polymerase L		PRO_0000079199
Experimental info
	Non-terminal residue	363	1

Sequences

	Sequence		Length	Mass (Da)
	P14241 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 7AD2BA6A8DC90A51	FASTA	363	41,607
	10 20 30 40 50 60 MDETIADLRE LCLNYIEQDE RLSRQKLNFL GQREPRMVLI EGLKLLSRCI EIDSADKSGC 70 80 90 100 110 120 IHNHDDKSVE TILIDSGIVC PGLPLIIPDG YKLIDNSLIL LECFVRSTPA SFEKKFIEDT 130 140 150 160 170 180 NKLACIKEDL AVAGITLVPI VDGRCDYDNS FMPEWVNFKF RDLLFKLLEY SSQDEKVFEE 190 200 210 220 230 240 SEYFRLCESL KTTVDKRSGM DSMKILKDAR SFHNDEIMKM CHDGVNPNMS CDDVVFGINS 250 260 270 280 290 300 FFGRFRRDLL NGKLKRNFQK VSPGGLIKEF SELYETLTDN DDILMLSKEP VESCPLMRFI 310 320 330 340 350 360 TAETHGHERG SDANTEYERL LSMLNKVKSL KLLNTRRRQL LNLDVLCPSS LIKQSISKGL END

References

[1]	"Conserved sequences and coding of two strains of lymphocytic choriomeningitis virus (WE and ARM) and Pichinde arenavirus." Romanowski V., Bishop D.H.L. Virus Res. 2:35-51(1985) [PubMed: 3984491] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M22016 Genomic RNA. Translation: AAA46266.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010453. RNA_pol_arenavir. [Graphical view]
Pfam	PF06317. Arena_RNA_pol. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_LYCVW

Accession

Primary (citable) accession number: P14241

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 31, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program