Reviewed, UniProtKB/Swiss-Prot P14234 (FGR_MOUSE)

Names and origin

Protein names

Recommended name:
    Proto-oncogene tyrosine-protein kinase Fgr
    EC=2.7.10.2
Alternative name(s):
    Proto-oncogene c-Fgr
    p55-Fgr

Gene names

Name:

Fgr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Binds PTPNS1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Disease	Proto-oncogene
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	defense response to Gram-positive bacterium Inferred from genetic interaction. Source: MGI protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Proto-oncogene tyrosine-protein kinase Fgr		PRO_0000088092
Regions
	Domain	65 – 126	62	SH3
	Domain	132 – 229	98	SH2
	Domain	251 – 504	254	Protein kinase
	Nucleotide binding	257 – 265	9	ATP By similarity
Sites
	Active site	370	1	Proton acceptor By similarity
	Binding site	279	1	ATP By similarity
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.4
	Modified residue	25	1	Phosphoserine By similarity
	Modified residue	32	1	Phosphotyrosine By similarity
	Modified residue	196	1	Phosphotyrosine By similarity
	Modified residue	400	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	511	1	Phosphotyrosine By similarity
	Modified residue	517	1	Phosphothreonine By similarity
Experimental info
	Sequence conflict	41	1	N → T in CAA36437. Ref.2
	Sequence conflict	212	1	Q → R in CAA36437. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P14234-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: F655BDB4510F3076	FASTA	517	58,868
	10 20 30 40 50 60 MGCVFCKKLE PASKEDVGLE GDFRSQTAEE RYYPDPTQGR NSSVFPQPTS PAFLNTGNMR 70 80 90 100 110 120 SISGTGVTIF VALYDYEART GDDLTFTKGE KFHILNNTEY DWWEARSLSS GHRGYVPSNY 130 140 150 160 170 180 VAPVDSIQAE EWYFGKISRK DAERQLLSSG NPQGAFLIRE SETTKGAYSL SIRDWDQNRG 190 200 210 220 230 240 DHIKHYKIRK LDTGGYYITT RAQFDSIQDL VQHYMEVNDG LCYLLTAPCT TTKPQTLGLA 250 260 270 280 290 300 KDAWEIDRNS IALERRLGTG CFGDVWLGTW NCSTKVAVKT LKPGTMSPKA FLEEAQIMKL 310 320 330 340 350 360 LRHDKLVQLY AVVSEEPIYI VTEFMCYGSL LDFLKDREGQ NLMLPHLVDM AAQVAEGMAY 370 380 390 400 410 420 MERMNYIHRD LRAANILVGE YLICKIADFG LARLIEDNEY NPQQGTKFPI KWTAPEAALF 430 440 450 460 470 480 GRFTVKSDVW SFGILLTELI TKGRVPYPGM NNREVLEQVE HGYHMPCPPG CPASLYEVME 490 500 510 QAWRLDPEER PTFEYLQSFL EDYFTSTEPQ YQPGDQT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of the murine c-fgr proto-oncogene cDNA and induction of c-fgr expression by proliferation and activation factors in normal bone marrow-derived monocytic cells." Yi T.L., Willman C.L. Oncogene 4:1081-1087(1989) [PubMed: 2674853] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: DBA/2J.
[2]	"Molecular cloning and sequencing of the murine c-fgr gene." King F.J., Cole M.D. Oncogene 5:337-344(1990) [PubMed: 2179817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Monocytic leukemia.
[3]	"Negative regulation of phagocytosis in murine macrophages by the Src kinase family member, Fgr." Gresham H.D., Dale B.M., Potter J.W., Chang P.W., Vines C.M., Lowell C.A., Lagenaur C.F., Willman C.L. J. Exp. Med. 191:515-528(2000) [PubMed: 10662797] [Abstract] Cited for: INTERACTION WITH PTPNS1.
[4]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13 AND TYR-400, MASS SPECTROMETRY. Tissue: Macrophage.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16440 mRNA. Translation: CAA34463.1. X52191 mRNA. Translation: CAA36437.1.
IPI	IPI00123708.
PIR	A43807.
UniGene	Mm.271665
3D structure databases
SMR	P14234. Positions 67-515.
ModBase	Search...
Protein-protein interaction databases
STRING	P14234.
PTM databases
PhosphoSite	P14234.
Proteomic databases
PRIDE	P14234.
Genome annotation databases
Ensembl	ENSMUST00000030693; ENSMUSP00000030693; ENSMUSG00000028874; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:95527. Fgr.
Phylogenomic databases
eggNOG	maNOG09357.
HOGENOM	HBG755340.
HOVERGEN	P14234.
InParanoid	P14234.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244.
Gene expression databases
ArrayExpress	P14234.
Bgee	P14234.
Genevestigator	P14234.
GermOnline	ENSMUSG00000028874. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR020473. SH3_region. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR020746. Tyr_prot_kinase_non-rcpt_Fgr. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256. Tyr_prot_kinase. 1 hit. PTHR23256:SF257. Tyr_prot_kinase_non-rcpt_Fgr. 1 hit.
Pfam	PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

FGR_MOUSE

Accession

Primary (citable) accession number: P14234
Secondary accession number(s): Q61404

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	February 9, 2010
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)