P14231 (AT1B2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-2

Alternative name(s):
AMOG
Glial cell adhesion molecule
Sodium/potassium-dependent ATPase subunit beta-2

Gene names

Name:

Atp1b2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Seems to mediate cell adhesion of neurons and astrocytes.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Cell adhesion Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sodium:potassium-exchanging ATPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Sodium/potassium-transporting ATPase subunit beta-2		PRO_0000219105
Regions
	Topological domain	1 – 39	39	Cytoplasmic Potential
	Transmembrane	40 – 67	28	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	68 – 290	223	Extracellular Potential
Amino acid modifications
	Glycosylation	96	1	N-linked (GlcNAc...) Potential
	Glycosylation	118	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	193	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
	Glycosylation	220	1	N-linked (GlcNAc...) Potential
	Glycosylation	238	1	N-linked (GlcNAc...) Ref.7
	Glycosylation	250	1	N-linked (GlcNAc...) Ref.7
	Disulfide bond	129 ↔ 150		By similarity
	Disulfide bond	160 ↔ 177		By similarity
	Disulfide bond	200 ↔ 261		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14231 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 731836AFB23198D4	FASTA	290	33,344
	10 20 30 40 50 60 MVIQKEKKSC GQVVEEWKEF VWNPRTHQFM GRTGTSWAFI LLFYLVFYGF LTAMFSLTMW 70 80 90 100 110 120 VMLQTVSDHT PKYQDRLATP GLMIRPKTEN LDVIVNISDT ESWGQHVQKL NKFLEPYNDS 130 140 150 160 170 180 IQAQKNDVCR PGRYYEQPDN GVLNYPKRAC QFNRTQLGDC SGIGDPTHYG YSTGQPCVFI 190 200 210 220 230 240 KMNRVINFYA GANQSMNVTC VGKRDEDAEN LGHFVMFPAN GSIDLMYFPY YGKKFHVNYT 250 260 270 280 290 QPLVAVKFLN VTPNVEVNVE CRINAANIAT DDERDKFAGR VAFKLRINKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The adhesion molecule on glia (AMOG) is a homologue of the beta subunit of the Na,K-ATPase." Gloor S.M., Antonicek H., Sweadner K., Pagliusi S., Frank R., Moos M., Schachner M. J. Cell Biol. 110:165-174(1990) [PubMed: 1688561] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Brain.
[2]	"Genomic structure of the adhesion molecule on glia (AMOG, Na/K-ATPase beta 2 subunit)." Magyar J.P., Schachner M. Nucleic Acids Res. 18:6695-6696(1990) [PubMed: 1701244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6J. Tissue: Brain.
[3]	Magyar J.P. Submitted (MAY-1992) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[4]	"Evolution of the Na,K- and H,K-ATPase beta subunit gene family: structure of the murine Na,K-ATPase beta 2 subunit gene." Shyjan A.W., Canfield V.A., Levenson R. Genomics 11:435-442(1991) [PubMed: 1663071] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Retina.
[6]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 9-25; 155-181 AND 248-274, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[7]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-118; ASN-238 AND ASN-250, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16645 mRNA. Translation: CAA34638.1. X56007 Genomic DNA. Translation: CAA39482.1. M60897 Genomic DNA. No translation available. BC042467 mRNA. Translation: AAH42467.1. BC058763 mRNA. Translation: AAH58763.1.
IPI	IPI00123704.
PIR	A34057. S14235.
RefSeq	NP_038201.1. NM_013415.5.
UniGene	Mm.235204.
3D structure databases
ProteinModelPortal	P14231.
SMR	P14231. Positions 23-289.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14231. 1 interaction.
STRING	P14231.
PTM databases
PhosphoSite	P14231.
Proteomic databases
PRIDE	P14231.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047889; ENSMUSP00000047353; ENSMUSG00000041329.
GeneID	11932.
KEGG	mmu:11932.
Organism-specific databases
CTD	482.
MGI	MGI:88109. Atp1b2.
Phylogenomic databases
eggNOG	roNOG04706.
HOGENOM	HBG446583.
HOVERGEN	HBG050603.
InParanoid	P14231.
OMA	TCVGKRD.
OrthoDB	EOG46144C.
PhylomeDB	P14231.
Gene expression databases
ArrayExpress	P14231.
Bgee	P14231.
CleanEx	MM_ATP1B2.
Genevestigator	P14231.
GermOnline	ENSMUSG00000041329. Mus musculus.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. PS00391. ATPASE_NA_K_BETA_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280021.
SOURCE	Search...

Entry information

Entry name

AT1B2_MOUSE

Accession

Primary (citable) accession number: P14231

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	February 1, 1996
Last modified:	November 16, 2011
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program