Reviewed, UniProtKB/Swiss-Prot P14230 (MTSM_SERMA)

Names and origin

Protein names

Recommended name:
    Modification methylase SmaI
      Short name=M.SmaI
    EC=2.1.1.113
Alternative name(s):
    N-4 cytosine-specific methyltransferase SmaI

Gene names

Name:

smaIM

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA cytosine = S-adenosyl-L-homocysteine + DNA N(4)-methylcytosine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	DNA methylation on cytosine Inferred from electronic annotation. Source: InterPro DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (cytosine-N4-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Modification methylase SmaI		PRO_0000087932

Sequences

	Sequence		Length	Mass (Da)
	P14230-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: A923C42E5DBAB5AF	FASTA	292	33,466
	10 20 30 40 50 60 MKKHSNNLDL FDQTEECLES NLRSCKIIVG DAREAVQGLD SEIFDCVVTS PPYWGLRDYG 70 80 90 100 110 120 NGGQIGAEDN INDYIKDLVD LFRDVRRTLK DDGTLWLNIG DSYTSGGRTW RDKDDKNKGR 130 140 150 160 170 180 AMSYRPPTPE GLKPKDLIGV PWRLAFALQN DGWYLRTDII WNKPNCQPES VRDRPTRSHE 190 200 210 220 230 240 YIFLLSKGKK YYYDWESIKE PASDPKMDKK NRRTVWNINT EPYPGSHFAV FPRAMARLCV 250 260 270 280 290 LAGSRPGGKV LDPFFGSGTT GVVCQELDRE CVGIELNEEY ASLAKERILR RR

References

[1]	"Cloning, characterization and heterologous expression of the SmaI restriction-modification system." Heidmann S., Seifert W., Kessler C., Domdey H. Nucleic Acids Res. 17:9783-9796(1989) [PubMed: 2690008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BMTU 1373.
[2]	"M.Smal is an N4-methylcytosine specific DNA-methylase." Klimasauskas S., Steponaviciene D., Maneliene Z., Petrusyte M., Butkus V., Janulaitis A. Nucleic Acids Res. 18:6607-6609(1990) [PubMed: 2251121] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Characterization of the SmaI restriction and modification enzymes." Dunbar J.C., Withers B. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16458 Genomic DNA. Translation: CAA34479.1. M98769 Genomic DNA. Translation: AAA26570.1.
PIR	S06036.
3D structure databases
HSSP	HSSP built from PDB template 1G60 based on UniProtKB P23192.
ModBase	Search...
Protein family/group databases
REBASE	3505. M.SmaI.
Enzyme and pathway databases
BRENDA	2.1.1.113. 457.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR001091. MeTrfase_CN4. IPR017985. MeTrfase_CN4_CS. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00093. N4_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTSM_SERMA

Accession

Primary (citable) accession number: P14230

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)