P14229 (T2SM_SERMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme SmaI
Short name=R.SmaI
EC=3.1.21.4

Alternative name(s):
Endonuclease SmaI
Type II restriction enzyme SmaI

Gene names

Name:

smaIR

Organism

Serratia marcescens

Taxonomic identifier

615 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Serratia

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CCCGGG and cleaves after C-3.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium. Potassium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 247	247	Type-2 restriction enzyme SmaI		PRO_0000077362

Sequences

	Sequence		Length	Mass (Da)
	P14229 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: DBE6CD66362DCF93	FASTA	247	28,782
	10 20 30 40 50 60 MSRDDQLFTL WGKLNDRQKD NFLKWMKAFD VEKTYQKTSG DIFNDDFFDI FGDRLITHHF 70 80 90 100 110 120 SSTQALTKTL FEHAFNDSLN ESGVISSLAE SRTNPGHDIT IDSIKVALKT EAAKNISKSY 130 140 150 160 170 180 IHVSKWMELG KGEWILELLL ERFLEHLENY ERIFTLRYFK ISEYKFSYQL VEIPKSLLLE 190 200 210 220 230 240 AKNAKLEIMS GSKQSPKPGY GYVLDENENK KFSLYFDGGA ERKLQIKHLN LEHCIVHGVW DFILPPP

References

[1]	"Cloning, characterization and heterologous expression of the SmaI restriction-modification system." Heidmann S., Seifert W., Kessler C., Domdey H. Nucleic Acids Res. 17:9783-9796(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BMTU 1373.
[2]	"Characterization of the SmaI restriction and modification enzymes." Dunbar J.C., Withers B. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16458 Genomic DNA. Translation: CAA34478.1. M98769 Genomic DNA. Translation: AAA26569.1.
PIR	S06035.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	1704. SmaI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2SM_SERMA

Accession

Primary (citable) accession number: P14229

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program