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P14229

- T2SM_SERMA

UniProt

P14229 - T2SM_SERMA

Protein

Type-2 restriction enzyme SmaI

Gene

smaIR

Organism
Serratia marcescens
Status
Reviewed - Annotation score: 2 out of 5- Protein predictedi
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    • History
      Entry version 61 (16 Apr 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence CCCGGG and cleaves after C-3.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.
    Potassium.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1704. SmaI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme SmaI (EC:3.1.21.4)
    Short name:
    R.SmaI
    Alternative name(s):
    Endonuclease SmaI
    Type II restriction enzyme SmaI
    Gene namesi
    Name:smaIR
    OrganismiSerratia marcescens
    Taxonomic identifieri615 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 247247Type-2 restriction enzyme SmaIPRO_0000077362Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P14229-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRDDQLFTL WGKLNDRQKD NFLKWMKAFD VEKTYQKTSG DIFNDDFFDI    50
    FGDRLITHHF SSTQALTKTL FEHAFNDSLN ESGVISSLAE SRTNPGHDIT 100
    IDSIKVALKT EAAKNISKSY IHVSKWMELG KGEWILELLL ERFLEHLENY 150
    ERIFTLRYFK ISEYKFSYQL VEIPKSLLLE AKNAKLEIMS GSKQSPKPGY 200
    GYVLDENENK KFSLYFDGGA ERKLQIKHLN LEHCIVHGVW DFILPPP 247
    Length:247
    Mass (Da):28,782
    Last modified:January 1, 1990 - v1
    Checksum:iDBE6CD66362DCF93
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16458 Genomic DNA. Translation: CAA34478.1.
    M98769 Genomic DNA. Translation: AAA26569.1.
    PIRiS06035.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16458 Genomic DNA. Translation: CAA34478.1 .
    M98769 Genomic DNA. Translation: AAA26569.1 .
    PIRi S06035.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1704. SmaI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning, characterization and heterologous expression of the SmaI restriction-modification system."
      Heidmann S., Seifert W., Kessler C., Domdey H.
      Nucleic Acids Res. 17:9783-9796(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BMTU 1373.
    2. "Characterization of the SmaI restriction and modification enzymes."
      Dunbar J.C., Withers B.
      Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiT2SM_SERMA
    AccessioniPrimary (citable) accession number: P14229
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: April 16, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3