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Protein

Type-2 restriction enzyme SmaI

Gene

smaIR

Organism
Serratia marcescens
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CCCGGG and cleaves after C-3.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Protein has several cofactor binding sites:

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BRENDAi3.1.21.4. 5690.

Protein family/group databases

REBASEi1704. SmaI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme SmaI (EC:3.1.21.4)
Short name:
R.SmaI
Alternative name(s):
Endonuclease SmaI
Type II restriction enzyme SmaI
Gene namesi
Name:smaIR
OrganismiSerratia marcescens
Taxonomic identifieri615 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 247247Type-2 restriction enzyme SmaIPRO_0000077362Add
BLAST

Sequencei

Sequence statusi: Complete.

P14229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRDDQLFTL WGKLNDRQKD NFLKWMKAFD VEKTYQKTSG DIFNDDFFDI
60 70 80 90 100
FGDRLITHHF SSTQALTKTL FEHAFNDSLN ESGVISSLAE SRTNPGHDIT
110 120 130 140 150
IDSIKVALKT EAAKNISKSY IHVSKWMELG KGEWILELLL ERFLEHLENY
160 170 180 190 200
ERIFTLRYFK ISEYKFSYQL VEIPKSLLLE AKNAKLEIMS GSKQSPKPGY
210 220 230 240
GYVLDENENK KFSLYFDGGA ERKLQIKHLN LEHCIVHGVW DFILPPP
Length:247
Mass (Da):28,782
Last modified:January 1, 1990 - v1
Checksum:iDBE6CD66362DCF93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16458 Genomic DNA. Translation: CAA34478.1.
M98769 Genomic DNA. Translation: AAA26569.1.
PIRiS06035.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16458 Genomic DNA. Translation: CAA34478.1.
M98769 Genomic DNA. Translation: AAA26569.1.
PIRiS06035.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi1704. SmaI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.21.4. 5690.

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "Cloning, characterization and heterologous expression of the SmaI restriction-modification system."
    Heidmann S., Seifert W., Kessler C., Domdey H.
    Nucleic Acids Res. 17:9783-9796(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BMTU 1373.
  2. "Characterization of the SmaI restriction and modification enzymes."
    Dunbar J.C., Withers B.
    Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiT2SM_SERMA
AccessioniPrimary (citable) accession number: P14229
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 1, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.