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P14229 (T2SM_SERMA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme SmaI

Short name=R.SmaI
EC=3.1.21.4
Alternative name(s):
Endonuclease SmaI
Type II restriction enzyme SmaI
Gene names
Name:smaIR
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CCCGGG and cleaves after C-3.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Potassium.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 247247Type-2 restriction enzyme SmaI
PRO_0000077362

Sequences

Sequence LengthMass (Da)Tools
P14229 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: DBE6CD66362DCF93

FASTA24728,782
        10         20         30         40         50         60 
MSRDDQLFTL WGKLNDRQKD NFLKWMKAFD VEKTYQKTSG DIFNDDFFDI FGDRLITHHF 

        70         80         90        100        110        120 
SSTQALTKTL FEHAFNDSLN ESGVISSLAE SRTNPGHDIT IDSIKVALKT EAAKNISKSY 

       130        140        150        160        170        180 
IHVSKWMELG KGEWILELLL ERFLEHLENY ERIFTLRYFK ISEYKFSYQL VEIPKSLLLE 

       190        200        210        220        230        240 
AKNAKLEIMS GSKQSPKPGY GYVLDENENK KFSLYFDGGA ERKLQIKHLN LEHCIVHGVW 


DFILPPP 

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References

[1]"Cloning, characterization and heterologous expression of the SmaI restriction-modification system."
Heidmann S., Seifert W., Kessler C., Domdey H.
Nucleic Acids Res. 17:9783-9796(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BMTU 1373.
[2]"Characterization of the SmaI restriction and modification enzymes."
Dunbar J.C., Withers B.
Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X16458 Genomic DNA. Translation: CAA34478.1.
M98769 Genomic DNA. Translation: AAA26569.1.
PIRS06035.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1704. SmaI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2SM_SERMA
AccessionPrimary (citable) accession number: P14229
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries