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Protein

Glycophorin-A

Gene

Gypa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Glycophorin A is the major intrinsic membrane sialoglycoprotein of the erythrocyte. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane (By similarity).By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

GO - Biological processi

  • cytoskeletal anchoring at plasma membrane Source: MGI
  • regulation of response to osmotic stress Source: MGI
Complete GO annotation...

Keywords - Ligandi

Sialic acid

Names & Taxonomyi

Protein namesi
Recommended name:
Glycophorin-A
Alternative name(s):
CD_antigen: CD235a
Gene namesi
Name:Gypa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:95880. Gypa.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 108108ExtracellularAdd
BLAST
Transmembranei109 – 13123Helical; Signal-anchor for type III membrane proteinAdd
BLAST
Topological domaini132 – 16837CytoplasmicAdd
BLAST

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

The amount of O-linked oligosaccharide chains in the erythrocyte membrane decreases to 60% compared to that of the wild-type mice. Erythrocytes lacking GPA are more sensitive to hypo-osmotic stress.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Glycophorin-APRO_0000149048Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei155 – 1551PhosphoserineBy similarity
Modified residuei160 – 1601PhosphothreonineCombined sources
Modified residuei165 – 1651PhosphoserineBy similarity
Modified residuei166 – 1661PhosphoserineCombined sources

Post-translational modificationi

The N-terminal extracellular domain is heavily glycosylated on serine and threonine residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP14220.

PTM databases

PhosphoSiteiP14220.
UniCarbKBiP14220.

Expressioni

Gene expression databases

BgeeiP14220.
CleanExiMM_GYPA.
ExpressionAtlasiP14220. baseline and differential.
GenevisibleiP14220. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070836.

Structurei

3D structure databases

ProteinModelPortaliP14220.
SMRiP14220. Positions 100-137.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycophorin-A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410JM5B. Eukaryota.
ENOG4111AH6. LUCA.
InParanoidiP14220.
KOiK06575.
OMAiPNEESSN.
OrthoDBiEOG7ZWD4D.
TreeFamiTF338555.

Family and domain databases

InterProiIPR001195. Glycophorin.
IPR018938. Glycophorin_CS.
[Graphical view]
PANTHERiPTHR13813. PTHR13813. 1 hit.
PIRSFiPIRSF002466. Glycophorin. 1 hit.
PROSITEiPS00312. GLYCOPHORIN_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14220-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTESTAAVTT SGHSLTTTFH IPSSQHYQEE HSPSLSGSDS LLQITTPVVA
60 70 80 90 100
STVGNPNQHS ATMSTPAIHV STYHTAPTEV SAAFEEQPVS PHIGGMPSPI
110 120 130 140 150
QHDFPALVMI LIILGVMAGI IGTILLISYC ISRMTKKSSV DIQSPEGGDN
160
SVPLSSIEQT PNEESSNV
Length:168
Mass (Da):17,664
Last modified:November 1, 1995 - v2
Checksum:i2DF15DDA3A587EC6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 10040ATMST…MPSPI → SAAFEEQPVSPHIGGMPSPI ATMSTPAIHVSTYHTAPTEV AA sequence (PubMed:2753361).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26385 mRNA. Translation: AAA37709.1.
M73815 Genomic DNA. Translation: AAA37708.1.
CCDSiCCDS40399.1.
PIRiJN0073.
RefSeqiNP_034499.3. NM_010369.3.
UniGeneiMm.13123.

Genome annotation databases

EnsembliENSMUST00000063359; ENSMUSP00000070836; ENSMUSG00000051839.
GeneIDi14934.
KEGGimmu:14934.
UCSCiuc009miz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M26385 mRNA. Translation: AAA37709.1.
M73815 Genomic DNA. Translation: AAA37708.1.
CCDSiCCDS40399.1.
PIRiJN0073.
RefSeqiNP_034499.3. NM_010369.3.
UniGeneiMm.13123.

3D structure databases

ProteinModelPortaliP14220.
SMRiP14220. Positions 100-137.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000070836.

PTM databases

PhosphoSiteiP14220.
UniCarbKBiP14220.

Proteomic databases

PaxDbiP14220.

Protocols and materials databases

DNASUi14934.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063359; ENSMUSP00000070836; ENSMUSG00000051839.
GeneIDi14934.
KEGGimmu:14934.
UCSCiuc009miz.1. mouse.

Organism-specific databases

CTDi2993.
MGIiMGI:95880. Gypa.

Phylogenomic databases

eggNOGiENOG410JM5B. Eukaryota.
ENOG4111AH6. LUCA.
InParanoidiP14220.
KOiK06575.
OMAiPNEESSN.
OrthoDBiEOG7ZWD4D.
TreeFamiTF338555.

Miscellaneous databases

NextBioi287251.
PROiP14220.
SOURCEiSearch...

Gene expression databases

BgeeiP14220.
CleanExiMM_GYPA.
ExpressionAtlasiP14220. baseline and differential.
GenevisibleiP14220. MM.

Family and domain databases

InterProiIPR001195. Glycophorin.
IPR018938. Glycophorin_CS.
[Graphical view]
PANTHERiPTHR13813. PTHR13813. 1 hit.
PIRSFiPIRSF002466. Glycophorin. 1 hit.
PROSITEiPS00312. GLYCOPHORIN_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the cDNA clone for mouse glycophorin, erythroid-specific membrane protein."
    Matsui Y., Natori S., Obinata M.
    Gene 77:325-332(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 138-146.
    Strain: BALB/cJ.
  2. "Full length mouse glycophorin gene constructed using recombinant polymerase chain reaction."
    Gu H., Planas J., Gomez R., Wilson D.J.
    Biochem. Biophys. Res. Commun. 177:202-208(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION.
  3. "Glycophorin A requirement for expression of O-linked antigens on the erythrocyte membrane."
    Arimitsu N., Akimitsu N., Kotani N., Takasaki S., Kina T., Hamamoto H., Kamura K., Sekimizu K.
    Genes Cells 8:769-777(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-160 AND SER-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Liver, Lung and Spleen.

Entry informationi

Entry nameiGLPA_MOUSE
AccessioniPrimary (citable) accession number: P14220
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1995
Last modified: March 16, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.