P14209 (CD99_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD99 antigen

Alternative name(s):
12E7
E2 antigen
Protein MIC2
T-cell surface glycoprotein E2
CD_antigen=CD99

Gene names

Name:	CD99
Synonyms:	MIC2, MIC2X, MIC2Y

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Post-translational modification	Extensively O-glycosylated.
Miscellaneous	The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.
Sequence similarities	Belongs to the CD99 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Traceable author statement. Source: ProtInc integral to plasma membrane Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform I (identifier: P14209-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II (identifier: P14209-2)

The sequence of this isoform differs from the canonical sequence as follows:
     159-185: AEQGEVDMESHRNANAEPAVQRTLLEK → DG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.1
	Chain	23 – 185	163	CD99 antigen		PRO_0000021726
Regions
	Topological domain	23 – 122	100	Extracellular Potential
	Transmembrane	123 – 147	25	Helical; Potential
	Topological domain	148 – 185	38	Cytoplasmic Potential
Natural variations
	Alternative sequence	159 – 185	27	AEQGE…TLLEK → DG in isoform II.		VSP_004324
	Natural variant	166	1	M → V. Corresponds to variant rs11556080 [ dbSNP | Ensembl ].		VAR_014733
	Natural variant	173	1	N → I. Corresponds to variant rs4717 [ dbSNP | Ensembl ].		VAR_014734

Sequences

	Sequence		Length	Mass (Da)
	Isoform I [UniParc]. Last modified January 1, 1990. Version 1. Checksum: C302E09E6B022EAB	FASTA	185	18,848
	10 20 30 40 50 60 MARGAALALL LFGLLGVLVA APDGGFDLSD ALPDNENKKP TAIPKKPSAG DDFDLGDAVV 70 80 90 100 110 120 DGENDDPRPP NPPKPMPNPN PNHPSSSGSF SDADLADGVS GGEGKGGSDG GGSHRKEGEE 130 140 150 160 170 180 ADAPGVIPGI VGAVVVAVAG AISSFIAYQK KKLCFKENAE QGEVDMESHR NANAEPAVQR TLLEK
	Isoform II [UniParc]. Checksum: 2BA54D31B410CAA8	FASTA	160	16,015
	10 20 30 40 50 60 MARGAALALL LFGLLGVLVA APDGGFDLSD ALPDNENKKP TAIPKKPSAG DDFDLGDAVV 70 80 90 100 110 120 DGENDDPRPP NPPKPMPNPN PNHPSSSGSF SDADLADGVS GGEGKGGSDG GGSHRKEGEE 130 140 150 160 ADAPGVIPGI VGAVVVAVAG AISSFIAYQK KKLCFKENDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product." Gelin C., Aubrit F., Phalipon A., Raynal B., Cole S., Kaczorek M., Bernard A. EMBO J. 8:3253-3259(1989) [PubMed: 2479542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM I), PROTEIN SEQUENCE OF 23-39. Tissue: T-cell.
[2]	"An alternative splicing form of CD99 (MIC2)." Park S.H., Hahn J.H., Kim M.K., Sohn H.W., Choi E.Y., Kim S.H. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM II).
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I).
[4]	"The DNA sequence of the human X chromosome." Ross M.T., Grafham D.V., Coffey A.J., Scherer S., McLay K., Muzny D., Platzer M., Howell G.R., Burrows C., Bird C.P., Frankish A., Lovell F.L., Howe K.L., Ashurst J.L., Fulton R.S., Sudbrak R., Wen G., Jones M.C. , Hurles M.E., Andrews T.D., Scott C.E., Searle S., Ramser J., Whittaker A., Deadman R., Carter N.P., Hunt S.E., Chen R., Cree A., Gunaratne P., Havlak P., Hodgson A., Metzker M.L., Richards S., Scott G., Steffen D., Sodergren E., Wheeler D.A., Worley K.C., Ainscough R., Ambrose K.D., Ansari-Lari M.A., Aradhya S., Ashwell R.I., Babbage A.K., Bagguley C.L., Ballabio A., Banerjee R., Barker G.E., Barlow K.F., Barrett I.P., Bates K.N., Beare D.M., Beasley H., Beasley O., Beck A., Bethel G., Blechschmidt K., Brady N., Bray-Allen S., Bridgeman A.M., Brown A.J., Brown M.J., Bonnin D., Bruford E.A., Buhay C., Burch P., Burford D., Burgess J., Burrill W., Burton J., Bye J.M., Carder C., Carrel L., Chako J., Chapman J.C., Chavez D., Chen E., Chen G., Chen Y., Chen Z., Chinault C., Ciccodicola A., Clark S.Y., Clarke G., Clee C.M., Clegg S., Clerc-Blankenburg K., Clifford K., Cobley V., Cole C.G., Conquer J.S., Corby N., Connor R.E., David R., Davies J., Davis C., Davis J., Delgado O., Deshazo D., Dhami P., Ding Y., Dinh H., Dodsworth S., Draper H., Dugan-Rocha S., Dunham A., Dunn M., Durbin K.J., Dutta I., Eades T., Ellwood M., Emery-Cohen A., Errington H., Evans K.L., Faulkner L., Francis F., Frankland J., Fraser A.E., Galgoczy P., Gilbert J., Gill R., Gloeckner G., Gregory S.G., Gribble S., Griffiths C., Grocock R., Gu Y., Gwilliam R., Hamilton C., Hart E.A., Hawes A., Heath P.D., Heitmann K., Hennig S., Hernandez J., Hinzmann B., Ho S., Hoffs M., Howden P.J., Huckle E.J., Hume J., Hunt P.J., Hunt A.R., Isherwood J., Jacob L., Johnson D., Jones S., de Jong P.J., Joseph S.S., Keenan S., Kelly S., Kershaw J.K., Khan Z., Kioschis P., Klages S., Knights A.J., Kosiura A., Kovar-Smith C., Laird G.K., Langford C., Lawlor S., Leversha M., Lewis L., Liu W., Lloyd C., Lloyd D.M., Loulseged H., Loveland J.E., Lovell J.D., Lozado R., Lu J., Lyne R., Ma J., Maheshwari M., Matthews L.H., McDowall J., McLaren S., McMurray A., Meidl P., Meitinger T., Milne S., Miner G., Mistry S.L., Morgan M., Morris S., Mueller I., Mullikin J.C., Nguyen N., Nordsiek G., Nyakatura G., O'dell C.N., Okwuonu G., Palmer S., Pandian R., Parker D., Parrish J., Pasternak S., Patel D., Pearce A.V., Pearson D.M., Pelan S.E., Perez L., Porter K.M., Ramsey Y., Reichwald K., Rhodes S., Ridler K.A., Schlessinger D., Schueler M.G., Sehra H.K., Shaw-Smith C., Shen H., Sheridan E.M., Shownkeen R., Skuce C.D., Smith M.L., Sotheran E.C., Steingruber H.E., Steward C.A., Storey R., Swann R.M., Swarbreck D., Tabor P.E., Taudien S., Taylor T., Teague B., Thomas K., Thorpe A., Timms K., Tracey A., Trevanion S., Tromans A.C., d'Urso M., Verduzco D., Villasana D., Waldron L., Wall M., Wang Q., Warren J., Warry G.L., Wei X., West A., Whitehead S.L., Whiteley M.N., Wilkinson J.E., Willey D.L., Williams G., Williams L., Williamson A., Williamson H., Wilming L., Woodmansey R.L., Wray P.W., Yen J., Zhang J., Zhou J., Zoghbi H., Zorilla S., Buck D., Reinhardt R., Poustka A., Rosenthal A., Lehrach H., Meindl A., Minx P.J., Hillier L.W., Willard H.F., Wilson R.K., Waterston R.H., Rice C.M., Vaudin M., Coulson A., Nelson D.L., Weinstock G., Sulston J.E., Durbin R.M., Hubbard T., Gibbs R.A., Beck S., Rogers J., Bentley D.R. Nature 434:325-337(2005) [PubMed: 15772651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I). Tissue: Brain, Kidney, Skin and Uterus.
[7]	"Molecular genetics of MIC2: a gene shared by the human X and Y chromosomes." Darling S.M., Goodfellow P.J., Pym B., Banting G.S., Pritchard C., Goodfellow P.N. Cold Spring Harb. Symp. Quant. Biol. 51:205-212(1986) [PubMed: 3472717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-105.
[8]	"Absence of methylation of a CpG-rich region at the 5' end of the MIC2 gene on the active X, the inactive X, and the Y chromosome." Goodfellow P.J., Mondello C., Darling S.M., Pym B., Little P., Goodfellow P.N. Proc. Natl. Acad. Sci. U.S.A. 85:5605-5609(1988) [PubMed: 2456574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
[9]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X16996 mRNA. Translation: CAA34863.1. U82164 mRNA. Translation: AAB58501.1. CR450286 mRNA. Translation: CAG29282.1. AC006209 Genomic DNA. No translation available. CH471074 Genomic DNA. Translation: EAW98697.1. BC002584 mRNA. Translation: AAH02584.1. BC003147 mRNA. Translation: AAH03147.1. BC010109 mRNA. Translation: AAH10109.1. BC021620 mRNA. Translation: AAH21620.1. BC024310 mRNA. Translation: AAH24310.1. M16279 mRNA. Translation: AAA02999.1. J03841 Genomic DNA. Translation: AAA59848.1.
IPI	IPI00220117. IPI00253036.
PIR	A60592. S06786.
RefSeq	NP_001116370.1. NM_001122898.1. NP_002405.1. NM_002414.3.
UniGene	Hs.653349.
3D structure databases
ProteinModelPortal	P14209.
ModBase	Search...
Protein-protein interaction databases
IntAct	P14209. 3 interactions.
MINT	MINT-5004058.
STRING	P14209.
Polymorphism databases
DMDM	119049.
Proteomic databases
PRIDE	P14209.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000381192; ENSP00000370588; ENSG00000002586.
GeneID	4267.
KEGG	hsa:4267.
UCSC	uc004cqm.1. human. uc004cqo.1. human.
Organism-specific databases
CTD	4267.
GeneCards	GC0XP002602.
HGNC	HGNC:7082. CD99.
HPA	CAB000020. HPA035304.
MIM	313470. gene. 450000. gene.
neXtProt	NX_P14209.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG21228.
GeneTree	ENSGT00530000063944.
HOVERGEN	HBG067989.
InParanoid	P14209.
OMA	NDPVPPN.
OrthoDB	EOG40S0H8.
Gene expression databases
ArrayExpress	P14209.
Bgee	P14209.
CleanEx	HS_CD99.
Genevestigator	P14209.
GermOnline	ENSG00000002586. Homo sapiens.
Family and domain databases
InterPro	IPR022078. CD99L2. [Graphical view]
KO	K06520.
Pfam	PF12301. CD99L2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	16815.
PMAP-CutDB	P14209.
SOURCE	Search...

Entry information

Entry name

CD99_HUMAN

Accession

Primary (citable) accession number: P14209
Secondary accession number(s): O00518, Q6ICV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	January 25, 2012
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.