Reviewed, UniProtKB/Swiss-Prot P14209 (CD99_HUMAN)

Names and origin

Protein names

Recommended name:
    CD99 antigen
Alternative name(s):
    T-cell surface glycoprotein E2
    E2 antigen
    Protein MIC2
    12E7
    CD_antigen=CD99

Gene names

Name:	CD99
Synonyms:	MIC2, MIC2X, MIC2Y

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes.
Subcellular location	Membrane; Single-pass type I membrane proteinPotential.
Post-translational modification	Extensively O-glycosylated.
Miscellaneous	The gene encoding for this protein is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes.
Sequence similarities	Belongs to the CD99 family.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: ProtInc integral to plasma membrane Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform I (identifier: P14209-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II (identifier: P14209-2)

The sequence of this isoform differs from the canonical sequence as follows:
     159-185: AEQGEVDMESHRNANAEPAVQRTLLEK → DG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22
	Chain	23 – 185	163	CD99 antigen		PRO_0000021726
Regions
	Topological domain	23 – 122	100	Extracellular Potential
	Transmembrane	123 – 147	25	Potential
	Topological domain	148 – 185	38	Cytoplasmic Potential
Natural variations
	Alternative sequence	159 – 185	27	AEQGE…TLLEK → DG in isoform II.		VSP_004324
	Natural variant	166	1	M → V: dbSNP rs4793.		VAR_014733
	Natural variant	173	1	N → I: dbSNP rs4717.		VAR_014734

Sequences

	Sequence		Length	Mass (Da)
	Isoform I [UniParc]. Last modified January 1, 1990. Version 1. Checksum: C302E09E6B022EAB	FASTA	185	18,848
	10 20 30 40 50 60 MARGAALALL LFGLLGVLVA APDGGFDLSD ALPDNENKKP TAIPKKPSAG DDFDLGDAVV 70 80 90 100 110 120 DGENDDPRPP NPPKPMPNPN PNHPSSSGSF SDADLADGVS GGEGKGGSDG GGSHRKEGEE 130 140 150 160 170 180 ADAPGVIPGI VGAVVVAVAG AISSFIAYQK KKLCFKENAE QGEVDMESHR NANAEPAVQR TLLEK
	Isoform II [UniParc]. Checksum: 2BA54D31B410CAA8		160	16,015
	10 20 30 40 50 60 MARGAALALL LFGLLGVLVA APDGGFDLSD ALPDNENKKP TAIPKKPSAG DDFDLGDAVV 70 80 90 100 110 120 DGENDDPRPP NPPKPMPNPN PNHPSSSGSF SDADLADGVS GGEGKGGSDG GGSHRKEGEE 130 140 150 160 ADAPGVIPGI VGAVVVAVAG AISSFIAYQK KKLCFKENDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The E2 antigen, a 32 kd glycoprotein involved in T-cell adhesion processes, is the MIC2 gene product." Gelin C., Aubrit F., Phalipon A., Raynal B., Cole S., Kaczorek M., Bernard A. EMBO J. 8:3253-3259(1989) [PubMed: 2479542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, PARTIAL PROTEIN SEQUENCE OF 23-39. Tissue: T-cell.
[2]	"An alternative splicing form of CD99 (MIC2)." Park S.H., Hahn J.H., Kim M.K., Sohn H.W., Choi E.Y., Kim S.H. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ISOFORM II).
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I). Tissue: Brain, Kidney, Skin and Uterus.
[4]	"Molecular genetics of MIC2: a gene shared by the human X and Y chromosomes." Darling S.M., Goodfellow P.J., Pym B., Banting G.S., Pritchard C., Goodfellow P.N. Cold Spring Harb. Symp. Quant. Biol. 51:205-212(1986) [PubMed: 3472717] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-105.
[5]	"Absence of methylation of a CpG-rich region at the 5' end of the MIC2 gene on the active X, the inactive X, and the Y chromosome." Goodfellow P.J., Mondello C., Darling S.M., Pym B., Little P., Goodfellow P.N. Proc. Natl. Acad. Sci. U.S.A. 85:5605-5609(1988) [PubMed: 2456574] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X16996 mRNA. Translation: CAA34863.1. U82164 mRNA. Translation: AAB58501.1. BC002584 mRNA. Translation: AAH02584.1. BC003147 mRNA. Translation: AAH03147.1. BC010109 mRNA. Translation: AAH10109.1. BC021620 mRNA. Translation: AAH21620.1. BC024310 mRNA. Translation: AAH24310.1. M16279 mRNA. Translation: AAA02999.1. J03841 Genomic DNA. Translation: AAA59848.1.
PIR	A60592. S06786.
RefSeq	NP_001116370.1. NP_002405.1.
UniGene	Hs.654354
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P14209.
Genome annotation databases
Ensembl	ENSG00000002586. Homo sapiens. [Contig view]
GeneID	4267.
KEGG	hsa:4267.
Organism-specific databases
H-InvDB	HIX0016631.
HGNC	HGNC:7082. CD99.
HPA	CAB000020.
MIM	313470. gene. 450000. gene.
PharmGKB	PA30804.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P14209.
HOVERGEN	P14209.
Gene expression databases
ArrayExpress	P14209.
CleanEx	HS_CD99.
GermOnline	ENSG00000002586. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
LinkHub	P14209.
NextBio	16815.
SOURCE	Search...

Entry information

Entry name

CD99_HUMAN

Accession

Primary (citable) accession number: P14209
Secondary accession number(s): O00518

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	November 25, 2008
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)