Reviewed, UniProtKB/Swiss-Prot P14204 (CMPA_BACSU)

Names and origin

Protein names

Recommended name:
    Transcriptional regulatory protein comA
Alternative name(s):
    Competence protein A

Gene names

Name:	comA
Synonyms:	comA1, comAA
Ordered Locus Names:	BSU31680

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Response regulator in the two-component regulatory system comP/comA involved in a major quorum response pathway that regulates the development of genetic competence. Regulates directly the expression of over 20 genes, including genes of the srfA operon, degQ, rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the regulation of comK transcription, the expression of late competence genes. Ref.4
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by comP.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Competence Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 214	214	Transcriptional regulatory protein comA		PRO_0000081075
Regions
	Domain	3 – 121	119	Response regulatory
	Domain	147 – 212	66	HTH luxR-type
	DNA binding	171 – 190	20	H-T-H motif By similarity
Amino acid modifications
	Modified residue	55	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14204-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 52D01A9740759072	FASTA	214	24,129
	10 20 30 40 50 60 MKKILVIDDH PAVMEGTKTI LETDSNLSVD CLSPEPSEQF IKQHDFSSYD LILMDLNLGG 70 80 90 100 110 120 EVNGMELSKQ ILQENPHCKI IVYTGYEVED YFEEAIRAGL HGAISKTESK EKITQYIYHV 130 140 150 160 170 180 LNGEILVDFA YFKQLMTQQK TKPAPSSQKE QDVLTPRECL ILQEVEKGFT NQEIADALHL 190 200 210 SKRSIEYSLT SIFNKLNVGS RTEAVLIAKS DGVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants." Weinrauch Y., Guillen N., Dubnau D. J. Bacteriol. 171:5362-5375(1989) [PubMed: 2507523] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)." Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T. Microbiology 143:2769-2774(1997) [PubMed: 9274030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis." Comella N., Grossman A.D. Mol. Microbiol. 57:1159-1174(2005) [PubMed: 16091051] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M22856 Genomic DNA. Translation: AAA22320.1. Z93932 Genomic DNA. Translation: CAB07904.1. AL009126 Genomic DNA. Translation: CAB15156.1.
PIR	RGBSCA. A33591.
RefSeq	NP_391046.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937179.
GenomeReviews	Gene locus BSU31680 in contig AL009126_GR.
KEGG	bsu:BSU31680.
Organism-specific databases
SubtiList	BG10381. comA. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P14204.
OMA	P14204. AIRHAVK.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3163-MON.
Family and domain databases
InterPro	IPR001789. Sig_transdc_resp-reg_receiver. IPR000792. Tscrpt_reg_LuxR_C. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF00196. GerE. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PRINTS	PR00038. HTHLUXR.
ProDom	PD000307. HTH_LuxR. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00421. HTH_LUXR. 1 hit. SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS00622. HTH_LUXR_1. 1 hit. PS50043. HTH_LUXR_2. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CMPA_BACSU

Accession

Primary (citable) accession number: P14204

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)