Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P14204

- CMPA_BACSU

UniProt

P14204 - CMPA_BACSU

Protein

Transcriptional regulatory protein ComA

Gene

comA

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Response regulator in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Regulates directly the expression of over 20 genes, including genes of the srfA operon, degQ, rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the regulation of comK transcription, the expression of late competence genes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi171 – 19020H-T-H motifPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. phosphorelay response regulator activity Source: InterPro
    3. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. establishment of competence for transformation Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Competence, Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU31680-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcriptional regulatory protein ComA
    Alternative name(s):
    Competence protein A
    Gene namesi
    Name:comA
    Synonyms:comA1, comAA
    Ordered Locus Names:BSU31680
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU31680. [Micado]

    Subcellular locationi

    Cytoplasm Curated

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 214214Transcriptional regulatory protein ComAPRO_0000081075Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei55 – 5514-aspartylphosphatePROSITE-ProRule annotation

    Post-translational modificationi

    Phosphorylated by ComP.

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP14204.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-59430N.
    IntActiP14204. 1 interaction.
    STRINGi224308.BSU31680.

    Structurei

    Secondary structure

    1
    214
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi150 – 1545
    Helixi156 – 16611
    Helixi171 – 1788
    Helixi182 – 19514
    Helixi201 – 2088

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KRFNMR-A/B146-214[»]
    3ULQX-ray2.30B146-214[»]
    ProteinModelPortaliP14204.
    SMRiP14204. Positions 2-214.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP14204.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 121119Response regulatoryPROSITE-ProRule annotationAdd
    BLAST
    Domaini147 – 21266HTH luxR-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 HTH luxR-type DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 response regulatory domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG2197.
    HOGENOMiHOG000034813.
    KOiK07691.
    OMAiQRSIEYT.
    OrthoDBiEOG69GZGV.
    PhylomeDBiP14204.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR011006. CheY-like_superfamily.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR000792. Tscrpt_reg_LuxR_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00196. GerE. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view]
    PRINTSiPR00038. HTHLUXR.
    SMARTiSM00421. HTH_LUXR. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view]
    SUPFAMiSSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEiPS00622. HTH_LUXR_1. 1 hit.
    PS50043. HTH_LUXR_2. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14204-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKILVIDDH PAVMEGTKTI LETDSNLSVD CLSPEPSEQF IKQHDFSSYD    50
    LILMDLNLGG EVNGMELSKQ ILQENPHCKI IVYTGYEVED YFEEAIRAGL 100
    HGAISKTESK EKITQYIYHV LNGEILVDFA YFKQLMTQQK TKPAPSSQKE 150
    QDVLTPRECL ILQEVEKGFT NQEIADALHL SKRSIEYSLT SIFNKLNVGS 200
    RTEAVLIAKS DGVL 214
    Length:214
    Mass (Da):24,129
    Last modified:January 1, 1990 - v1
    Checksum:i52D01A9740759072
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22856 Genomic DNA. Translation: AAA22320.1.
    Z93932 Genomic DNA. Translation: CAB07904.1.
    AL009126 Genomic DNA. Translation: CAB15156.1.
    PIRiA33591. RGBSCA.
    RefSeqiNP_391046.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15156; CAB15156; BSU31680.
    GeneIDi937179.
    KEGGibsu:BSU31680.
    PATRICi18978306. VBIBacSub10457_3316.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M22856 Genomic DNA. Translation: AAA22320.1 .
    Z93932 Genomic DNA. Translation: CAB07904.1 .
    AL009126 Genomic DNA. Translation: CAB15156.1 .
    PIRi A33591. RGBSCA.
    RefSeqi NP_391046.1. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KRF NMR - A/B 146-214 [» ]
    3ULQ X-ray 2.30 B 146-214 [» ]
    ProteinModelPortali P14204.
    SMRi P14204. Positions 2-214.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-59430N.
    IntActi P14204. 1 interaction.
    STRINGi 224308.BSU31680.

    Proteomic databases

    PaxDbi P14204.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15156 ; CAB15156 ; BSU31680 .
    GeneIDi 937179.
    KEGGi bsu:BSU31680.
    PATRICi 18978306. VBIBacSub10457_3316.

    Organism-specific databases

    GenoListi BSU31680. [Micado ]

    Phylogenomic databases

    eggNOGi COG2197.
    HOGENOMi HOG000034813.
    KOi K07691.
    OMAi QRSIEYT.
    OrthoDBi EOG69GZGV.
    PhylomeDBi P14204.

    Enzyme and pathway databases

    BioCyci BSUB:BSU31680-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P14204.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR011006. CheY-like_superfamily.
    IPR016032. Sig_transdc_resp-reg_C-effctor.
    IPR001789. Sig_transdc_resp-reg_receiver.
    IPR000792. Tscrpt_reg_LuxR_C.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00196. GerE. 1 hit.
    PF00072. Response_reg. 1 hit.
    [Graphical view ]
    PRINTSi PR00038. HTHLUXR.
    SMARTi SM00421. HTH_LUXR. 1 hit.
    SM00448. REC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46894. SSF46894. 1 hit.
    SSF52172. SSF52172. 1 hit.
    PROSITEi PS00622. HTH_LUXR_1. 1 hit.
    PS50043. HTH_LUXR_2. 1 hit.
    PS50110. RESPONSE_REGULATORY. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants."
      Weinrauch Y., Guillen N., Dubnau D.
      J. Bacteriol. 171:5362-5375(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)."
      Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T.
      Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis."
      Comella N., Grossman A.D.
      Mol. Microbiol. 57:1159-1174(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiCMPA_BACSU
    AccessioniPrimary (citable) accession number: P14204
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3