P14204 (CMPA_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcriptional regulatory protein ComA

Alternative name(s):
Competence protein A

Gene names

Name:	comA
Synonyms:	comA1, comAA
Ordered Locus Names:	BSU31680

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	214 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Response regulator in the two-component regulatory system ComP/ComA involved in a major quorum response pathway that regulates the development of genetic competence. Regulates directly the expression of over 20 genes, including genes of the srfA operon, degQ, rapA, rapC, rapE, rapF, etc. Regulates indirectly, through the regulation of comK transcription, the expression of late competence genes. Ref.4
Subcellular location	Cytoplasm Probable.
Post-translational modification	Phosphorylated by ComP.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Competence Transcription Transcription regulation Two-component regulatory system
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of competence for transformation Inferred from electronic annotation. Source: UniProtKB-KW intracellular signal transduction Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 214

214

Transcriptional regulatory protein ComA

PRO_0000081075

Regions

Domain

3 – 121

119

Response regulatory

Domain

147 – 212

66

HTH luxR-type

DNA binding

171 – 190

20

H-T-H motif By similarity

Amino acid modifications

Modified residue

55

1

4-aspartylphosphate By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

214

Helix Strand Turn

Details...

Beta strand

150 – 154

5

Helix

156 – 166

11

Helix

171 – 178

8

Helix

182 – 195

14

Helix

201 – 208

8

Sequences

	Sequence		Length	Mass (Da)
	P14204 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 52D01A9740759072	FASTA	214	24,129
	10 20 30 40 50 60 MKKILVIDDH PAVMEGTKTI LETDSNLSVD CLSPEPSEQF IKQHDFSSYD LILMDLNLGG 70 80 90 100 110 120 EVNGMELSKQ ILQENPHCKI IVYTGYEVED YFEEAIRAGL HGAISKTESK EKITQYIYHV 130 140 150 160 170 180 LNGEILVDFA YFKQLMTQQK TKPAPSSQKE QDVLTPRECL ILQEVEKGFT NQEIADALHL 190 200 210 SKRSIEYSLT SIFNKLNVGS RTEAVLIAKS DGVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and transcription mapping of Bacillus subtilis competence genes comB and comA, one of which is related to a family of bacterial regulatory determinants." Weinrauch Y., Guillen N., Dubnau D. J. Bacteriol. 171:5362-5375(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Bacillus subtilis genome: cloning and nucleotide sequence of a 62 kb region between 275 degrees (rrnB) and 284 degrees (pai)." Oudega B., Koningstein G., Rodrigues L., de Sales Ramon M., Hilbert H., Duesterhoeft A., Pohl T.M., Weitzenegger T. Microbiology 143:2769-2774(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Conservation of genes and processes controlled by the quorum response in bacteria: characterization of genes controlled by the quorum-sensing transcription factor ComA in Bacillus subtilis." Comella N., Grossman A.D. Mol. Microbiol. 57:1159-1174(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M22856 Genomic DNA. Translation: AAA22320.1.
Z93932 Genomic DNA. Translation: CAB07904.1.
AL009126 Genomic DNA. Translation: CAB15156.1.

PIR

RGBSCA. A33591.

RefSeq

NP_391046.1. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KRF	NMR	-	A/B	146-214	[»]
3ULQ	X-ray	2.30	B	146-214	[»]

ProteinModelPortal

P14204.

SMR

P14204. Positions 2-214.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59430N.

IntAct

P14204. 1 interaction.

STRING

224308.BSU31680.

Proteomic databases

PaxDb

P14204.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB15156; CAB15156; BSU31680.

GeneID

937179.

KEGG

bsu:BSU31680.

PATRIC

18978306. VBIBacSub10457_3316.

Organism-specific databases

GenoList

BSU31680. [Micado]

Phylogenomic databases

eggNOG

COG2197.

HOGENOM

HOG000034813.

KO

K07691.

OMA

YDVYLID.

ProtClustDB

CLSK887804.

Enzyme and pathway databases

BioCyc

BSUB:BSU31680-MONOMER.

Family and domain databases

Gene3D

1.10.10.10. 1 hit.

InterPro

IPR011006. CheY-like_superfamily.
IPR001789. Sig_transdc_resp-reg_receiver.
IPR000792. Tscrpt_reg_LuxR_C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]

Pfam

PF00196. GerE. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]

PRINTS

PR00038. HTHLUXR.

SMART

SM00421. HTH_LUXR. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]

SUPFAM

SSF52172. CheY_like. 1 hit.

PROSITE

PS00622. HTH_LUXR_1. 1 hit.
PS50043. HTH_LUXR_2. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P14204.

Entry information

Entry name

CMPA_BACSU

Accession

Primary (citable) accession number: P14204

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program