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Protein

Pro-MCH

Gene

Pmch

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MCH inhibits ACTH secretion at the end of the light on period which corresponds to the peak of the circadian rhythm in ACTH. Inhibits also stress induced ACTH release during the light off period of the cycle. Involved as a neurotransmitter or neuromodulator in a broad array of neuronal functions. Stimulates sexual behavior when injected into the ventromedial nucleus, this effect is antagonized by NEI. In the medial preoptic area, stimulates anxiety and sexual behavior. Antagonizes inhibitory effect of melanotropin alpha on exploration behavior.
NEI can influence differentiation of neuronal processes in brain neurons. Affects the content of neurofilament protein in neuritogenesis (in vitro). May also be a neuromodulatory factor. In behavioral tests, it stimulates exploration and anxiety when injected into the ventromedial nucleus. Also stimulates grooming, locomotion and rearing. May antagonize the inhibitory effect of mch on ACTH release. Reduces dopamine and dopac release in the ventromedial nucleus.

GO - Molecular functioni

  • type 1 melanin-concentrating hormone receptor binding Source: RGD

GO - Biological processi

  • chemical synaptic transmission Source: InterPro
  • drinking behavior Source: RGD
  • feeding behavior Source: UniProtKB
  • glucose homeostasis Source: RGD
  • lactation Source: RGD
  • learning or memory Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of synaptic transmission, dopaminergic Source: RGD
  • neuropeptide signaling pathway Source: UniProtKB-KW
  • positive regulation of circadian sleep/wake cycle, REM sleep Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • regulation of heart rate Source: RGD
  • regulation of neuronal synaptic plasticity Source: RGD
  • response to cold Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hormone, Neuropeptide

Names & Taxonomyi

Protein namesi
Recommended name:
Pro-MCH
Cleaved into the following 3 chains:
Neuropeptide-glycine-glutamic acid
Short name:
NGE
Short name:
Neuropeptide G-E
Neuropeptide-glutamic acid-isoleucine
Short name:
NEI
Short name:
Neuropeptide E-I
Gene namesi
Name:Pmch
Synonyms:Mch
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3358. Pmch.

Subcellular locationi

GO - Cellular componenti

  • cell Source: GOC
  • extracellular space Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 165144Pro-MCHPRO_0000019116Add
BLAST
Peptidei110 – 12819Neuropeptide-glycine-glutamic acidSequence analysisPRO_0000019117Add
BLAST
Peptidei131 – 14313Neuropeptide-glutamic acid-isoleucinePRO_0000019118Add
BLAST
Peptidei147 – 16519Melanin-concentrating hormonePRO_0000019119Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei143 – 1431Isoleucine amide1 Publication
Disulfide bondi153 ↔ 162By similarity

Post-translational modificationi

Pro-MCH is processed differentially in the brain and in peripheral organs producing two neuropeptides; NEI and MCH. A third peptide, NGE, may also be produced. Preferential processing in neurons by prohormone convertase 2 (PC2) generates NEI. MCH is generated in neurons of the lateral hypothalmic area by several prohormone convertases including PC1/3, PC2 and PC5/6.2 Publications

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP14200.
PRIDEiP14200.

Miscellaneous databases

PMAP-CutDBP14200.

Expressioni

Tissue specificityi

MCH is present in all regions of the brain and in neurointermediate lobe of the pituarity gland, with highest concentrations in the hypothalamus. Also expressed to a much lesser extent in stomach, lamina propria of both duodenum and colon, ovary, thymus, pancreas, adrenal gland and testis (spermatogonia, early spermatocytes and Sertoli cells). Weak expression in heart and lung. The other peptides are expressed at least in Sertoli cells, nei being also expressed in brain, stomach and proximal duodenum. In brain exclusively mature mch and nei peptides are present. In peripheral tissues a large product, encompassing the NEI and MCH domains of the precursor, is found predominantly. At low levels fully processed MCH and NEI peptides are present in gut. No expression in peripheral blood.3 Publications

Developmental stagei

Expression is strongly increased in hypothalamus between postnatal days 12 and 20, to reach high constant values in adult.

Inductioni

Inhibited by neurogenic stress or osmotic stress.

Interactioni

GO - Molecular functioni

  • type 1 melanin-concentrating hormone receptor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006174.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IW1Y. Eukaryota.
ENOG410XWB7. LUCA.
HOGENOMiHOG000230520.
HOVERGENiHBG052429.
InParanoidiP14200.
KOiK05229.
PhylomeDBiP14200.

Family and domain databases

InterProiIPR005456. Prepro-melanin_conc_hormone.
[Graphical view]
PANTHERiPTHR12091. PTHR12091. 1 hit.
PfamiPF05824. Pro-MCH. 1 hit.
[Graphical view]
PRINTSiPR01641. PROMCHFAMILY.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14200-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKMSLSSYM LMLAFSLFSH GILLSASKSI RNVEDDIVFN TFRMGKAFQK
60 70 80 90 100
EDTAERSVVA PSLEGYKNDE SGFMKDDDDK TTKNTGSKQN LVTHGLPLSL
110 120 130 140 150
AVKPYLALKG PAVFPAENGV QNTESTQEKR EIGDEENSAK FPIGRRDFDM
160
LRCMLGRVYR PCWQV
Length:165
Mass (Da):18,482
Last modified:January 1, 1990 - v1
Checksum:i38E9F07693E77A05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29712 mRNA. Translation: AAA41580.1.
M62641 Genomic DNA. Translation: AAA41581.1.
PIRiA36237.
RefSeqiNP_036757.1. NM_012625.1.
UniGeneiRn.9675.

Genome annotation databases

GeneIDi24659.
KEGGirno:24659.
UCSCiRGD:3358. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29712 mRNA. Translation: AAA41580.1.
M62641 Genomic DNA. Translation: AAA41581.1.
PIRiA36237.
RefSeqiNP_036757.1. NM_012625.1.
UniGeneiRn.9675.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000006174.

Proteomic databases

PaxDbiP14200.
PRIDEiP14200.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24659.
KEGGirno:24659.
UCSCiRGD:3358. rat.

Organism-specific databases

CTDi5367.
RGDi3358. Pmch.

Phylogenomic databases

eggNOGiENOG410IW1Y. Eukaryota.
ENOG410XWB7. LUCA.
HOGENOMiHOG000230520.
HOVERGENiHBG052429.
InParanoidiP14200.
KOiK05229.
PhylomeDBiP14200.

Miscellaneous databases

PMAP-CutDBP14200.
PROiP14200.

Family and domain databases

InterProiIPR005456. Prepro-melanin_conc_hormone.
[Graphical view]
PANTHERiPTHR12091. PTHR12091. 1 hit.
PfamiPF05824. Pro-MCH. 1 hit.
[Graphical view]
PRINTSiPR01641. PROMCHFAMILY.
ProtoNetiSearch...

Entry informationi

Entry nameiMCH_RAT
AccessioniPrimary (citable) accession number: P14200
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.