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Protein

Protein ref(2)P

Gene

ref(2)P

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for selective autophagy activation by ubiquitinated proteins (PubMed:22622177). Implicated in sigma rhabdovirus multiplication and necessary for male fertility. Involved in activating transcription of Drs (PubMed:12446795, PubMed:2510997).3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 167ZZ-typePROSITE-ProRule annotationAdd BLAST47

GO - Molecular functioni

GO - Biological processi

  • mitochondrion organization Source: FlyBase
  • selective autophagy Source: UniProtKB
  • sperm mitochondrion organization Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • ubiquitin-dependent protein catabolic process Source: GO_Central

Keywordsi

Molecular functionDNA-binding
Biological processTranscription
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ref(2)P
Alternative name(s):
Refractory to sigma P
Gene namesi
Name:ref(2)P
ORF Names:CG10360
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0003231 ref(2)P

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Males are sterile, spermatids exhibit degenerated mitochondria. Reduced activation of the Drs promoter. Double mutants for ref2P and scny die 96 hours after egg laying (PubMed:22622177).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000972371 – 599Protein ref(2)PAdd BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei447Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP14199
PRIDEiP14199

PTM databases

iPTMnetiP14199

Expressioni

Gene expression databases

BgeeiFBgn0003231
GenevisibleiP14199 DM

Interactioni

Subunit structurei

Interacts with aPKC and Traf6.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi6122340 interactors.
DIPiDIP-18088N
IntActiP14199 9 interactors.
MINTiP14199
STRINGi7227.FBpp0080794

Structurei

3D structure databases

ProteinModelPortaliP14199
SMRiP14199
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 88PB1PROSITE-ProRule annotationAdd BLAST86
Repeati386 – 39318
Repeati399 – 40628
Repeati407 – 41337
Domaini550 – 595UBAPROSITE-ProRule annotationAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni386 – 4133 X 8 AA repeats of S-A-N-Q-S-X-X-PAdd BLAST28

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 167ZZ-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IQZA Eukaryota
ENOG410XWHN LUCA
InParanoidiP14199
OMAiQNDYEIF
OrthoDBiEOG091G08ES
PhylomeDBiP14199

Family and domain databases

CDDicd14320 UBA_SQSTM, 1 hit
InterProiView protein in InterPro
IPR000270 PB1_dom
IPR033741 SQSTM_UBA
IPR015940 UBA
IPR009060 UBA-like_sf
IPR000433 Znf_ZZ
PfamiView protein in Pfam
PF00564 PB1, 1 hit
PF16577 UBA_5, 1 hit
PF00569 ZZ, 1 hit
SMARTiView protein in SMART
SM00165 UBA, 1 hit
SM00291 ZnF_ZZ, 1 hit
SUPFAMiSSF46934 SSF46934, 1 hit
PROSITEiView protein in PROSITE
PS51745 PB1, 1 hit
PS50030 UBA, 1 hit
PS01357 ZF_ZZ_1, 1 hit
PS50135 ZF_ZZ_2, 1 hit

Sequencei

Sequence statusi: Complete.

P14199-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPEKLLKITY QGAGPQKKIN AYLRMPSQNY TILRREIELY LFQERQLPKC
60 70 80 90 100
DVRTFWIDAD KDEIEIVNQN DYEIFLAKCE SNMHVQVAPL APVEEPKATK
110 120 130 140 150
QEGSSANAEA PSVDDPSNFT IHDAVECDGC GLAPLIGFRY KCVQCSNYDL
160 170 180 190 200
CQKCELAHKH PEHLMLRMPT NNGPGMVDAW FTGPGLGRRS GRRSRGHCPF
210 220 230 240 250
QETNQADPAG EPARDSRRER RQARRHAGVL TQFVEMMTNL PLNTTTATAP
260 270 280 290 300
AEPQKPKAAE QTESPPQAEP TVTAEKAAES EAKPTEPKKV NTDQSVPRTE
310 320 330 340 350
DPVTTPRSTQ PTTPVINLDN ISQIVPPEYM SAGIEILNNF SEMFSKIIDT
360 370 380 390 400
TEGGDSGIFA PSTTPSAENK KPEEQGQSSG QSGASSANQS AVPSAAPSAN
410 420 430 440 450
QSNVPSANQS ATPSISGSIP DAQLETEPLN PKPSETTTET EQERRRSDSL
460 470 480 490 500
DPEWQLIDNA YSANNSNLIN LDTTNPTAAP QEPVRDFGQL GELLRQHMNE
510 520 530 540 550
EARVEQASAN TQTAQVDTVS TSTSTTSVTT NSVGTSPAAP DDKRTVPVYH
560 570 580 590
TDESINKSIH AMMAMGFSNE GAWLTQLLES VQGNISAALD VMNVSQNRN
Length:599
Mass (Da):65,303
Last modified:January 23, 2002 - v2
Checksum:i946043CE19AAF126
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti188R → S in CAA34861 (PubMed:2510997).Curated1

Polymorphismi

Polymorphic for two allelic forms in natural populations of Drosophila melanogaster, ref2Po and ref2Pp. The latter allele confers resistance to the rhabdovirus sigma infecting wild populations.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti28 – 29QN → G in BPL_1D, BPL_2B, BPL_3E, BPL_3F, BPL_5A, BPL_5D, BPL_5F, BPL_6D, BPL_6E, BPL_7F, BPL_8A, BPL_8C, BPL_9H, BPL_10D, BPL_10G, BPL_11D, BPL_12B, BPL_13A, BPL_13F, Florida1Slow and Paris. 3 Publications2
Natural varianti32I → A in strain: AfricaFast, BPL_1A, BPL_1B, BPL_1D, BPL_1G, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2G, BPL_2H, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_4A, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H, BPL_7C, BPL_7E, BPL_7F, BPL_8A, BPL_8C, BPL_8D, BPL_8E, BPL_8F, BPL_9A, BPL_9C, BPL_9F, BPL_9H, BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_11B, BPL_11C, BPL_11D, BPL_11E, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C, BPL_13A, BPL_13D, BPL_13F, FloridaFast, Florida1Slow, Paris and WashingtonFast. 3 Publications1
Natural varianti32I → M in strain: JapanSlow. 1 Publication1
Natural varianti32I → V in strain: BPL_1D, BPL_2B, BPL_3E, BPL_3F, BPL_9H, BPL_10D, BPL_10G, BPL_11D, BPL_12B and BPL_13A. 1
Natural varianti43Q → L in strain: AfricaFast, BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_11B, BPL_11C, BPL_11D, BPL_11E, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C, BPL_13A, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1D, BPL_1G, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2G, BPL_2H, BPL_3A, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_4A, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H, BPL_7C, BPL_7E, BPL_7F, BPL_8A, BPL_8C, BPL_8D, BPL_8E, BPL_8F, BPL_9A, BPL_9C, BPL_9F, BPL_9H, Florida1Slow, FloridaFast, JapanSlow, Oregon-R, Paris and WashingtonFast. 4 Publications1
Natural varianti60D → Y in strain: BPL_1A, BPL_1G, BPL_2A, BPL_2G, BPL_2H, BPL_3C, BPL_4A, BPL_4C, BPL_4G, BPL_5H, BPL_6H, BPL_7C, BPL_10E, BPL_11C, BPL_11H, BPL_12A and WashingtonFast. 2 Publications1
Natural varianti79C → R in strain: BPL_1B, BPL_9F and BPL_13D. 1 Publication1
Natural varianti186 – 192Missing in strain: Paris. 2 Publications7
Natural varianti231T → S in strain: BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_10H, BPL_11B, BPL_11C, BPL_11D, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C, BPL_13A, BPL_13C, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1C, BPL_1D, BPL_1E, BPL_1G, BPL_1H, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2G, BPL_2H, BPL_3A, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_3G, BPL_3H, BPL_4A, BPL_4B, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5D, BPL_5E, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H, BPL_7B, BPL_7C, BPL_7E, BPL_7F, BPL_7H, BPL_8A, BPL_8C, BPL_8D, BPL_8E, BPL_8F, BPL_8G, BPL_9A, BPL_9C, BPL_9F, BPL_9H, FrenchSlow and Paris. 3 Publications1
Natural varianti247A → D in strain: BPL_7A, BPL_9D, BPL_10A and BPL_11E. 1 Publication1
Natural varianti274A → V in strain: BPL_1E. 1 Publication1
Natural varianti290V → D in strain: BPL_3G. 1 Publication1
Natural varianti331S → R in strain: AfricaFast, AfricaSlow, BPL_10A, BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_10H, BPL_11B, BPL_11C, BPL_11D, BPL_11E, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12B, BPL_12C, BPL_12F, BPL_13A, BPL_13B, BPL_13C, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1C, BPL_1D, BPL_1E, BPL_1F, BPL_1G, BPL_1H, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2F, BPL_2G, BPL_2H, BPL_3A, BPL_3B, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_3G, BPL_3H, BPL_4A, BPL_4B, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5B, BPL_5D, BPL_5E, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6E, BPL_6F, BPL_6H, BPL_7A, BPL_7B, BPL_7C, BPL_7E, BPL_7F, BPL_7G, BPL_7H, BPL_8A, BPL_8B, BPL_8C, BPL_8D, BPL_8E, BPL_8F, BPL_8G, BPL_8H, BPL_9A, BPL_9B, BPL_9C, BPL_9D, BPL_9F, BPL_9H, Florida1Slow, Florida2Slow, FloridaFast, FrenchFast, FrenchSlow, JapanFast, JapanSlow, Paris and WashingtonFast. 3 Publications1
Natural varianti356S → L in strain: BPL_6H, BPL_7C, BPL_11F and BPL_12A. 1 Publication1
Natural varianti389Q → E in strain: BPL_1C. 1 Publication1
Natural varianti393P → L in strain: BPL_1H, BPL_3H, BPL_4B, BPL_5E, BPL_7B, BPL_7H, BPL_8G, BPL_10H and BPL_13C. 1 Publication1
Natural varianti397P → S in strain: FrenchSlow. 1 Publication1
Natural varianti403 – 410Missing in strain: AfricaFast, BPL_10C, BPL_10D, BPL_10E, BPL_10G, BPL_11B, BPL_11D, BPL_11F, BPL_11G, BPL_11H, BPL_12A, BPL_12C, BPL_13A, BPL_13D, BPL_13F, BPL_1A, BPL_1B, BPL_1G, BPL_2A, BPL_2B, BPL_2C, BPL_2D, BPL_2E, BPL_2G, BPL_2H, BPL_3A, BPL_3C, BPL_3D, BPL_3E, BPL_3F, BPL_4A, BPL_4C, BPL_4E, BPL_4G, BPL_5A, BPL_5B, BPL_5D, BPL_5F, BPL_5G, BPL_5H, BPL_6A, BPL_6B, BPL_6D, BPL_6F, BPL_6H, BPL_7C, BPL_7E, BPL_7F, BPL_7G, BPL_8A, BPL_8C, BPL_8D, BPL_8E, BPL_9A, BPL_9C, BPL_9F, BPL_9H, Florida1Slow, Florida2Slow, FloridaFast, Paris and WashingtonFast. 3 Publications8
Natural varianti432K → M in strain: FrenchFast. 2 Publications1
Natural varianti444R → I in strain: BPL_3B and BPL_8F. 1 Publication1
Natural varianti542D → E in strain: AfricaSlow, AfricaFast, FloridaFast, Florida1Slow, Florida2Slow, FrenchSlow, JapanSlow, Paris and WashingtonFast. 2 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16993 Genomic DNA Translation: CAA34861.1
X69828 Genomic DNA Translation: CAA49482.1
X69829 mRNA Translation: CAA49483.1
X69830 mRNA Translation: CAA49484.1
U23923 Genomic DNA Translation: AAA98832.1
U23924 Genomic DNA Translation: AAA98833.1
U23925 Genomic DNA Translation: AAA98834.1
U23926 Genomic DNA Translation: AAA98835.1
U23927 Genomic DNA Translation: AAA98836.1
U23928 Genomic DNA Translation: AAA98837.1
U23929 Genomic DNA Translation: AAA98838.1
U23931 Genomic DNA Translation: AAA98839.1
U23932 Genomic DNA Translation: AAA98840.1
U23933 Genomic DNA Translation: AAA98841.1
AE014134 Genomic DNA Translation: AAF53824.1
AE014134 Genomic DNA Translation: AAX52671.1
AY089494 mRNA Translation: AAL90232.1
AM114104 Genomic DNA Translation: CAJ34995.2
AM114105 Genomic DNA Translation: CAJ34996.2
AM114106 Genomic DNA Translation: CAJ34997.2
AM114107 Genomic DNA Translation: CAJ34998.2
AM114108 Genomic DNA Translation: CAJ34999.2
AM114109 Genomic DNA Translation: CAJ35000.2
AM114110 Genomic DNA Translation: CAJ35001.2
AM114111 Genomic DNA Translation: CAJ35002.2
AM114112 Genomic DNA Translation: CAJ35003.2
AM114113 Genomic DNA Translation: CAJ35004.2
AM114114 Genomic DNA Translation: CAJ35005.2
AM114115 Genomic DNA Translation: CAJ35006.2
AM114116 Genomic DNA Translation: CAJ35007.2
AM114117 Genomic DNA Translation: CAJ35008.2
AM114118 Genomic DNA Translation: CAJ35009.2
AM114119 Genomic DNA Translation: CAJ35010.2
AM114120 Genomic DNA Translation: CAJ35011.1
AM114120 Genomic DNA Translation: CAJ38290.1
AM114121 Genomic DNA Translation: CAJ35012.2
AM114122 Genomic DNA Translation: CAJ35013.2
AM114123 Genomic DNA Translation: CAJ35014.2
AM114124 Genomic DNA Translation: CAJ35015.2
AM114125 Genomic DNA Translation: CAJ35016.2
AM114126 Genomic DNA Translation: CAJ35017.2
AM114127 Genomic DNA Translation: CAJ35018.2
AM114128 Genomic DNA Translation: CAJ35019.2
AM114129 Genomic DNA Translation: CAJ35020.2
AM114130 Genomic DNA Translation: CAJ35021.2
AM114131 Genomic DNA Translation: CAJ35022.2
AM114132 Genomic DNA Translation: CAJ35023.2
AM114133 Genomic DNA Translation: CAJ35024.2
AM114134 Genomic DNA Translation: CAJ35025.2
AM114135 Genomic DNA Translation: CAJ35026.2
AM114136 Genomic DNA Translation: CAJ35027.2
AM114137 Genomic DNA Translation: CAJ35028.2
AM114138 Genomic DNA Translation: CAJ35029.2
AM114139 Genomic DNA Translation: CAJ35030.2
AM114140 Genomic DNA Translation: CAJ35031.2
AM114141 Genomic DNA Translation: CAJ35032.2
AM114142 Genomic DNA Translation: CAJ35033.2
AM114143 Genomic DNA Translation: CAJ35034.2
AM114144 Genomic DNA Translation: CAJ35035.2
AM114145 Genomic DNA Translation: CAJ35036.2
AM114146 Genomic DNA Translation: CAJ35037.2
AM114147 Genomic DNA Translation: CAJ35038.2
AM114148 Genomic DNA Translation: CAJ35039.2
AM114149 Genomic DNA Translation: CAJ35040.2
AM114150 Genomic DNA Translation: CAJ35041.2
AM114151 Genomic DNA Translation: CAJ35042.2
AM114152 Genomic DNA Translation: CAJ35043.2
AM114153 Genomic DNA Translation: CAJ35044.2
AM114154 Genomic DNA Translation: CAJ35045.2
AM114155 Genomic DNA Translation: CAJ35046.2
AM114156 Genomic DNA Translation: CAJ35047.2
AM114157 Genomic DNA Translation: CAJ35048.2
AM114158 Genomic DNA Translation: CAJ35049.2
AM114159 Genomic DNA Translation: CAJ35050.2
AM114160 Genomic DNA Translation: CAJ35051.2
AM114161 Genomic DNA Translation: CAJ35052.2
AM114162 Genomic DNA Translation: CAJ35053.2
AM114163 Genomic DNA Translation: CAJ35054.2
AM114164 Genomic DNA Translation: CAJ35055.1
AM114164 Genomic DNA Translation: CAJ38334.1
AM114165 Genomic DNA Translation: CAJ35056.2
AM114166 Genomic DNA Translation: CAJ35057.2
AM114167 Genomic DNA Translation: CAJ35058.2
AM114168 Genomic DNA Translation: CAJ35059.2
AM114169 Genomic DNA Translation: CAJ35060.2
AM114170 Genomic DNA Translation: CAJ35061.2
AM114171 Genomic DNA Translation: CAJ35062.2
AM114172 Genomic DNA Translation: CAJ35063.2
AM114173 Genomic DNA Translation: CAJ35064.2
AM114174 Genomic DNA Translation: CAJ35065.2
AM114175 Genomic DNA Translation: CAJ35066.2
AM114176 Genomic DNA Translation: CAJ35067.2
AM114177 Genomic DNA Translation: CAJ35068.2
AM114178 Genomic DNA Translation: CAJ35069.2
AM114179 Genomic DNA Translation: CAJ35070.2
AM114180 Genomic DNA Translation: CAJ35071.2
AM114181 Genomic DNA Translation: CAJ35072.2
AM114182 Genomic DNA Translation: CAJ35073.2
AM114183 Genomic DNA Translation: CAJ35074.2
AM114184 Genomic DNA Translation: CAJ35075.1
AM114184 Genomic DNA Translation: CAJ38354.1
AM114185 Genomic DNA Translation: CAJ35076.2
AM114186 Genomic DNA Translation: CAJ35077.2
AM114187 Genomic DNA Translation: CAJ35078.2
AM114188 Genomic DNA Translation: CAJ35079.1
AM114188 Genomic DNA Translation: CAJ38358.1
AM114189 Genomic DNA Translation: CAJ35080.2
AM114190 Genomic DNA Translation: CAJ35081.2
AM114191 Genomic DNA Translation: CAJ35082.2
PIRiS06785
S33562 S29961
S33563 S29963
S33564 S29964
RefSeqiNP_001014491.1, NM_001014491.2
NP_476700.1, NM_057352.4
UniGeneiDm.12210

Genome annotation databases

EnsemblMetazoaiFBtr0081253; FBpp0080794; FBgn0003231
FBtr0100289; FBpp0099683; FBgn0003231
GeneIDi35246
KEGGidme:Dmel_CG10360
UCSCiCG10360-RB d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiREF2P_DROME
AccessioniPrimary (citable) accession number: P14199
Secondary accession number(s): A6PX28
, A6PX29, A6PX30, A6PX31, A6PX32, A6PX33, A6PX35, A6PX37, A6PX38, A6PX44, A6PX45, A6PX46, A6PX51, A6PX56, A6PX61, A6PX72, A6PX73, A6PX89, A6PX90, A6PXA2, A6PXA4, A6PXB0, A6PXB1, Q04576, Q04577, Q04578, Q24063, Q24064, Q24065, Q24066, Q24067, Q24068, Q24069, Q24070, Q24071, Q24072, Q541D1, Q9VIU1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2002
Last modified: April 25, 2018
This is version 156 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome