P14194 (CTC_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
General stress protein CTC

Alternative name(s):
50S ribosomal protein L25

Gene names

Name:	ctc
Ordered Locus Names:	BSU00520

Organism

Bacillus subtilis

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Not required for exponential growth; probably functions in vegetatively growing cells, maybe required for accurate translation under stress conditions. HAMAP MF_01334
Subunit structure	Part of the ribosome (presumably the 50S subunit) under heat-stress but not control growth conditions. Binds 5S rRNA.
Developmental stage	Expressed at the end of exponential growth under conditions in which the enzymes of the TCA cycle are repressed. HAMAP MF_01334
Induction	By heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation. HAMAP MF_01334
Disruption phenotype	Cells sporulate poorly at 48 degrees Celsius. Ref.5
Sequence similarities	Belongs to the ribosomal protein L25P family. Ctc subfamily.

Ontologies

Keywords
Biological process	Stress response
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: InterPro
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5S rRNA binding Inferred from electronic annotation. Source: InterPro structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 204	203	General stress protein CTC HAMAP MF_01334		PRO_0000181512
Natural variations
	Natural variant	2	1	A → R in strain: IS58.
	Natural variant	10	1	T → Q in strain: IS58.
	Natural variant	13	1	T → I in strain: IS58.

Sequences

	Sequence		Length	Mass (Da)
	P14194 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: D72CEA9437C094E7	FASTA	204	22,056
	10 20 30 40 50 60 MATLTAKERT DFTRSSLRNI RTSGHVPGII YGKDTGNKPV SLDSVELIKT LRDEGKNAVI 70 80 90 100 110 120 TLEVSGEKHS VMVTDLQTDP LKNEITHADF QVVNMSEDIE VEVPIHLTGE AIGVKNGGVL 130 140 150 160 170 180 QQPLYALTVK AKPKAIPQTI EADISSLDVN EVLTIADLPA GGDYSFNHES DEVVASILPP 190 200 QQQEAAEVDE EESADAQPEG ENEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 180 kilobase region of the Bacillus subtilis chromosome containing the replication origin." Ogasawara N., Nakai S., Yoshikawa H. DNA Res. 1:1-14(1994) [PubMed: 7584024] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Primary structure of the tms and prs genes of Bacillus subtilis." Nilsson D., Hove-Jensen B., Arnvig K. Mol. Gen. Genet. 218:565-571(1989) [PubMed: 2555671] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-186.
[4]	"Analysis of the induction of general stress proteins of Bacillus subtilis." Voelker U., Engelmann S., Maul B., Riethdorf S., Voelker A., Schmid R., Mach H., Hecker M. Microbiology 140:741-752(1994) [PubMed: 8012595] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-14. Strain: 168 / IS58.
[5]	"Effects on growth and sporulation of inactivation of a Bacillus subtilis gene (ctc) transcribed in vitro by minor vegetative cell RNA polymerases (E-sigma 37, E-sigma 32)." Truitt C.L., Weaver E.A., Haldenwang W.G. Mol. Gen. Genet. 212:166-171(1988) [PubMed: 2836704] [Abstract] Cited for: DISRUPTION PHENOTYPE. Strain: 168 / 1A3.
[6]	"The general stress protein Ctc of Bacillus subtilis is a ribosomal protein." Schmalisch M., Langbein I., Stuelke J. J. Mol. Microbiol. Biotechnol. 4:495-501(2002) [PubMed: 12432960] [Abstract] Cited for: DETECTION IN RIBOSOMES, BINDING TO 5S RRNA. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[7]	"General stress protein CTC from Bacillus subtilis specifically binds to ribosomal 5S RNA." Korepanov A.P., Gongadze G.M., Garber M.B. Biokhimiia 69:607-611(2004) [PubMed: 15236599] [Abstract] Cited for: BINDING TO 5S RRNA.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D26185 Genomic DNA. Translation: BAA05287.1. AL009126 Genomic DNA. Translation: CAB11828.1. X16518 Genomic DNA. Translation: CAA34524.1.
PIR	S66082.
RefSeq	NP_387933.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P14194.
SMR	P14194. Positions 3-199.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBACT00000003366; EBBACP00000003366; EBBACG00000003359.
GeneID	936984.
GenomeReviews	Gene locus BSU00520 in contig AL009126_GR.
KEGG	bsu:BSU00520.
NMPDR	fig|224308.1.peg.52.
PATRIC	18971575. VBIBacSub10457_0053.
Organism-specific databases
GenoList	BSU00520. [Micado]
Phylogenomic databases
GeneTree	EBGT00050000002196.
HOGENOM	HBG731986.
OMA	YGGDKAP.
PhylomeDB	P14194.
ProtClustDB	PRK05618.
Enzyme and pathway databases
BioCyc	BSUB:BSU00520-MONOMER.
Family and domain databases
HAMAP	MF_01334. Ribosomal_L25_CTC. [Tree]
InterPro	IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. IPR011035. Ribosomal_L25/Gln-tRNA_synth. IPR020057. Ribosomal_L25_b-dom. IPR001021. Ribosomal_L25_long. IPR020055. Ribosomal_L25_short. [Graphical view]
Gene3D	G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit. G3DSA:2.170.120.20. Ribosomal_L25_b-dom. 1 hit.
KO	K02897.
Pfam	PF01386. Ribosomal_L25p. 1 hit. [Graphical view]
ProDom	PD012503. Ribosomal_L25. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF50715. Ribosomal_L25rel. 1 hit.
TIGRFAMs	TIGR00731. Ctc_TL5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CTC_BACSU

Accession

Primary (citable) accession number: P14194

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 98 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program