P14187 (CCHL_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c heme lyase
Short name=CCHL
EC=4.4.1.17

Alternative name(s):
Holocytochrome-c synthase

Gene names

Name:	cyt-2
ORF Names:	NCU05601

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Links covalently the heme group to the apoprotein of cytochrome c.
Catalytic activity	Holocytochrome c = apocytochrome c + heme.
Subcellular location	Mitochondrion inner membrane.
Sequence similarities	Belongs to the cytochrome c-type heme lyase family. Contains 2 HRM (heme regulatory motif) repeats.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Repeat
Ligand	Heme Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	holocytochrome-c synthase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Cytochrome c heme lyase		PRO_0000121715
Regions
	Repeat	34 – 39	6	HRM 1
	Repeat	66 – 71	6	HRM 2

Sequences

	Sequence		Length	Mass (Da)
	P14187 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 9A6167BF3A3C05D7	FASTA	346	38,147
	10 20 30 40 50 60 MGWFWADGNA SAAAPVVPPS HKDLAASGAV PPPSCPMHNK TMDALSAHKP VTPAPEPTPA 70 80 90 100 110 120 AAAPSKCPVN HGAKDTLAAA AAAVAPKQPQ PENHQPAAAS EPSFFSKLNP LNYMFSSISQ 130 140 150 160 170 180 EPAPNQAIAL PTERDPSSIP KGTGDGNWEY PSPQQMYNAL LRKGYTDTDI TAVESMVAVH 190 200 210 220 230 240 NFLNEGAWNE IVEWERRFGK GLMRGWEIMK RGEENAPMML RRLEAQENDP EPQPTLIRFQ 250 260 270 280 290 300 GRPKDMTPKA ALLQVLGRIN SKYATEPPFD RHDWYVSRDE NGQKKEVRYV IDFYSAPPEP 310 320 330 340 TGEPVFYLDV RPAVTVTGAC ERLLRWGGDV WWKASGGEVR ERERSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the Neurospora crassa gene for cytochrome c heme lyase." Drygas M.E., Lambowitz A.M., Nargang F.E. J. Biol. Chem. 264:17897-17906(1989) [PubMed: 2572587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05075 Genomic DNA. Translation: AAA33583.1. AABX02000022 Genomic DNA. Translation: EAA31327.1.
PIR	A34365.
RefSeq	XP_960563.1. XM_955470.1.
UniGene	Ncr.15828.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P14187.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000005563; EFNCRP00000005557; EFNCRG00000005553.
GeneID	3876678.
KEGG	ncr:NCU05601.
NMPDR	fig|5141.1.peg.3389.
Phylogenomic databases
eggNOG	fuNOG07296.
GeneTree	EFGT00050000005300.
OMA	IVEWERR.
OrthoDB	EOG4MSH77.
Enzyme and pathway databases
BioCyc	NCRA-XX3-01:NCRA-XX3-01-009349-MONOMER.
Family and domain databases
InterPro	IPR000511. Cyt_C/C1_haem_lyase. [Graphical view]
KO	K01764.
PANTHER	PTHR12743. Cyto_heme_lyase. 1 hit.
Pfam	PF01265. Cyto_heme_lyase. 1 hit. [Graphical view]
PROSITE	PS00821. CYTO_HEME_LYASE_1. 1 hit. PS00822. CYTO_HEME_LYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCHL_NEUCR

Accession

Primary (citable) accession number: P14187
Secondary accession number(s): Q7RVB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program