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Protein

Chitin synthase 2

Gene

CHS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential for septum formation and cell division. CHS2 is required for maintaining normal cell morphology.

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).

Enzyme regulationi

Requires proteolytic activation.

GO - Molecular functioni

  • chitin synthase activity Source: SGD

GO - Biological processi

  • actomyosin contractile ring contraction Source: SGD
  • barrier septum site selection Source: GO_Central
  • cell wall chitin biosynthetic process Source: GO_Central
  • chitin biosynthetic process Source: SGD
  • fungal-type cell wall organization Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciMetaCyc:YBR038W-MONOMER.
YEAST:YBR038W-MONOMER.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitin synthase 2 (EC:2.4.1.16)
Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 2
Gene namesi
Name:CHS2
Ordered Locus Names:YBR038W
ORF Names:YBR0407
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR038W.
SGDiS000000242. CHS2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 643ExtracellularSequence analysisAdd BLAST643
Transmembranei644 – 664HelicalSequence analysisAdd BLAST21
Topological domaini665 – 677CytoplasmicSequence analysisAdd BLAST13
Transmembranei678 – 698HelicalSequence analysisAdd BLAST21
Topological domaini699 – 711ExtracellularSequence analysisAdd BLAST13
Transmembranei712 – 732HelicalSequence analysisAdd BLAST21
Topological domaini733 – 743CytoplasmicSequence analysisAdd BLAST11
Transmembranei744 – 764HelicalSequence analysisAdd BLAST21
Topological domaini765 – 775ExtracellularSequence analysisAdd BLAST11
Transmembranei776 – 796HelicalSequence analysisAdd BLAST21
Topological domaini797 – 875CytoplasmicSequence analysisAdd BLAST79
Transmembranei876 – 896HelicalSequence analysisAdd BLAST21
Topological domaini897 – 905ExtracellularSequence analysis9
Transmembranei906 – 926HelicalSequence analysisAdd BLAST21
Topological domaini927 – 963CytoplasmicSequence analysisAdd BLAST37

GO - Cellular componenti

  • cell periphery Source: GO_Central
  • cell septum Source: GO_Central
  • cellular bud neck Source: SGD
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi197N → A: 30% decrease of activity. 1 Publication1
Mutagenesisi312S → A: 20% increase of activity. 1 Publication1
Mutagenesisi355D → A: 10% decrease of activity. 1 Publication1
Mutagenesisi393D → A: 5% decrease of activity. 1 Publication1
Mutagenesisi441D → A: Loss of activity. 1 Publication1
Mutagenesisi441D → E: Loss of activity. 1 Publication1
Mutagenesisi447N → A: 80% decrease of activity. 1 Publication1
Mutagenesisi490Q → A: 70% decrease of activity. 1 Publication1
Mutagenesisi492F → A: 80% decrease of activity. 1 Publication1
Mutagenesisi493E → A: 90% decrease of activity. 1 Publication1
Mutagenesisi494Y → A: 95% decrease of activity. 1 Publication1
Mutagenesisi497S → A: 95% decrease of activity. 1 Publication1
Mutagenesisi502K → A: 90% decrease of activity. 1 Publication1
Mutagenesisi505E → A: 80% decrease of activity. 1 Publication1
Mutagenesisi506S → A: 20% increase of activity. 1 Publication1
Mutagenesisi508F → A: 60% decrease of activity. 1 Publication1
Mutagenesisi509G → A: 70% decrease of activity. 1 Publication1
Mutagenesisi514L → A: 80% decrease of activity. 1 Publication1
Mutagenesisi515P → A: 90% decrease of activity. 1 Publication1
Mutagenesisi516G → A: 95% decrease of activity. 1 Publication1
Mutagenesisi521Y → A: Loss of activity. 1 Publication1
Mutagenesisi522R → A: 60% decrease of activity. 1 Publication1
Mutagenesisi537R → A: No change in activity. 1 Publication1
Mutagenesisi550H → A: 85% decrease of activity. 1 Publication1
Mutagenesisi556N → A: 95% decrease of activity. 1 Publication1
Mutagenesisi559L → A: 95% decrease of activity. 1 Publication1
Mutagenesisi561E → A: Loss of activity. 1 Publication1
Mutagenesisi561E → D: 65% decrease of activity. 1 Publication1
Mutagenesisi561E → Q: Loss of activity. 1 Publication1
Mutagenesisi562D → A: Loss of activity. 1 Publication1
Mutagenesisi562D → E: Loss of activity. 1 Publication1
Mutagenesisi562D → N: Loss of activity. 1 Publication1
Mutagenesisi563R → A: Loss of activity. 1 Publication1
Mutagenesisi563R → K: 94% decrease of activity. 1 Publication1
Mutagenesisi565L → A: 95% decrease of activity. 1 Publication1
Mutagenesisi589T → A: 70% decrease of activity. 1 Publication1
Mutagenesisi592P → A: 70% decrease of activity. 1 Publication1
Mutagenesisi601Q → A: Loss of activity. 1 Publication1
Mutagenesisi601Q → N: Loss of activity. 1 Publication1
Mutagenesisi602 – 603RR → KK: Loss of activity. 1 Publication2
Mutagenesisi602R → A: Loss of activity. 1 Publication1
Mutagenesisi602R → K: 95% decrease of activity. 1 Publication1
Mutagenesisi603R → A: Loss of activity. 1 Publication1
Mutagenesisi603R → K: 57% decrease of activity. 1 Publication1
Mutagenesisi604R → A: Loss of activity. 1 Publication1
Mutagenesisi604R → K: Loss of activity. 1 Publication1
Mutagenesisi605W → A: Loss of activity. 1 Publication1
Mutagenesisi605W → Y: Loss of activity. 1 Publication1
Mutagenesisi607N → A: 95% decrease of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001937281 – 963Chitin synthase 2Add BLAST963

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22N-linked (GlcNAc...)Sequence analysis1
Modified residuei40PhosphoserineCombined sources1
Modified residuei82PhosphoserineCombined sources1
Modified residuei86PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1
Modified residuei133PhosphoserineCombined sources1
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1
Modified residuei217PhosphoserineCombined sources1
Glycosylationi447N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP14180.
PRIDEiP14180.

PTM databases

iPTMnetiP14180.

Interactioni

Protein-protein interaction databases

BioGridi32739. 48 interactors.
DIPiDIP-3819N.
IntActiP14180. 17 interactors.
MINTiMINT-532106.

Structurei

3D structure databases

ProteinModelPortaliP14180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the chitin synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000113171.
HOGENOMiHOG000162144.
InParanoidiP14180.
KOiK00698.
OMAiPEFTEMR.
OrthoDBiEOG092C0R1Z.

Family and domain databases

InterProiIPR004835. Chitin_synth.
IPR004834. Chitin_synth_fun.
IPR013616. Chitin_synth_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22914. PTHR22914. 1 hit.
PfamiPF01644. Chitin_synth_1. 1 hit.
PF08407. Chitin_synth_1N. 1 hit.
[Graphical view]
ProDomiPD002998. Chitin_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53448. SSF53448. 3 hits.

Sequencei

Sequence statusi: Complete.

P14180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRNPFMVEP SNGSPNRRGA SNLSKFYANA NSNSRWANPS EESLEDSYDQ
60 70 80 90 100
SNVFQGLPAS PSRAALRYSP DRRHRTQFYR DSAHNSPVAP NRYAANLQES
110 120 130 140 150
PKRAGEAVIH LSEGSNLYPR DNADLPVDPY HLSPQQQPSN NLFGSGRLYS
160 170 180 190 200
QSSKYTMSTT STTAPSLAEA DDEKEKYLTS TTSYDDQSTI FSADTFNETK
210 220 230 240 250
FELNHPTRQQ YVRRANSESK RRMVSDLPPP SKKKALLKLD NPIPKGLLDT
260 270 280 290 300
LPRRNSPEFT EMRYTACTVE PDDFLREGYT LRFAEMNREC QIAICITMYN
310 320 330 340 350
EDKYSLARTI HSIMKNVAHL CKREKSHVWG PNGWKKVSVI LISDGRAKVN
360 370 380 390 400
QGSLDYLAAL GVYQEDMAKA SVNGDPVKAH IFELTTQVSI NADLDYVSKD
410 420 430 440 450
IVPVQLVFCL KEENKKKINS HRWLFNAFCP VLQPTVVTLV DVGTRLNNTA
460 470 480 490 500
IYRLWKVFDM DSNVAGAAGQ IKTMKGKWGL KLFNPLVASQ NFEYKISNIL
510 520 530 540 550
DKPLESVFGY ISVLPGALSA YRYRALKNHE DGTGPLRSYF LGETQEGRDH
560 570 580 590 600
DVFTANMYLA EDRILCWELV AKRDAKWVLK YVKEATGETD VPEDVSEFIS
610 620 630 640 650
QRRRWLNGAM FAAIYAQLHF YQIWKTKHSV VRKFFLHVEF LYQFIQMLFS
660 670 680 690 700
WFSIANFVLT FYYLAGSMNL VIKHGEALFI FFKYLIFCDL ASLFIISMGN
710 720 730 740 750
RPQGAKHLFI TSMVILSICA TYSLICGFVF AFKSLASGTE SHKIFVDIVI
760 770 780 790 800
SLLSTYGLYF FSSLMYLDPW HMFTSSIQYF LTLPAFTCTL QIFAFCNTHD
810 820 830 840 850
VSWGTKGSTQ ESKQLSKAIV VQGPDGKQIV ETDWPQEVDK KFLEIKSRLK
860 870 880 890 900
EPEFEESSGN EKQSKNDYYR DIRTRIVMIW MLSNLILIMS IIQVFTPQDT
910 920 930 940 950
DNGYLIFILW SVAALAAFRV VGSMAFLFMK YLRIIVSYRN KVEGSGSWEV
960
SKLDLPNVFH KKG
Length:963
Mass (Da):109,882
Last modified:January 1, 1990 - v1
Checksum:i4D44A287C0B65B5B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23865 Genomic DNA. Translation: AAA34493.1.
Z35907 Genomic DNA. Translation: CAA84980.1.
BK006936 Genomic DNA. Translation: DAA07158.1.
PIRiS45167.
RefSeqiNP_009594.1. NM_001178386.1.

Genome annotation databases

EnsemblFungiiYBR038W; YBR038W; YBR038W.
GeneIDi852326.
KEGGisce:YBR038W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23865 Genomic DNA. Translation: AAA34493.1.
Z35907 Genomic DNA. Translation: CAA84980.1.
BK006936 Genomic DNA. Translation: DAA07158.1.
PIRiS45167.
RefSeqiNP_009594.1. NM_001178386.1.

3D structure databases

ProteinModelPortaliP14180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32739. 48 interactors.
DIPiDIP-3819N.
IntActiP14180. 17 interactors.
MINTiMINT-532106.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

iPTMnetiP14180.

Proteomic databases

MaxQBiP14180.
PRIDEiP14180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR038W; YBR038W; YBR038W.
GeneIDi852326.
KEGGisce:YBR038W.

Organism-specific databases

EuPathDBiFungiDB:YBR038W.
SGDiS000000242. CHS2.

Phylogenomic databases

GeneTreeiENSGT00730000113171.
HOGENOMiHOG000162144.
InParanoidiP14180.
KOiK00698.
OMAiPEFTEMR.
OrthoDBiEOG092C0R1Z.

Enzyme and pathway databases

BioCyciMetaCyc:YBR038W-MONOMER.
YEAST:YBR038W-MONOMER.

Miscellaneous databases

PROiP14180.

Family and domain databases

InterProiIPR004835. Chitin_synth.
IPR004834. Chitin_synth_fun.
IPR013616. Chitin_synth_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22914. PTHR22914. 1 hit.
PfamiPF01644. Chitin_synth_1. 1 hit.
PF08407. Chitin_synth_1N. 1 hit.
[Graphical view]
ProDomiPD002998. Chitin_synth. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF53448. SSF53448. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiCHS2_YEAST
AccessioniPrimary (citable) accession number: P14180
Secondary accession number(s): D6VQ38
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 160 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.