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Protein

Glycine betaine/proline betaine transport system permease protein ProW

Gene

proW

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake (PubMed:3305496, PubMed:7898450, PubMed:23249124). Probably responsible for the translocation of the substrate across the membrane (Probable).Curated3 Publications

GO - Molecular functioni

  • transporter activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:PROW-MONOMER.
ECOL316407:JW2653-MONOMER.
MetaCyc:PROW-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine/proline betaine transport system permease protein ProWCurated
Gene namesi
Name:proW1 Publication
Ordered Locus Names:b2678, JW2653
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10772. proW.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 99Cytoplasmic1 PublicationAdd BLAST99
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Topological domaini121Periplasmic1 Publication1
Transmembranei122 – 142HelicalSequence analysisAdd BLAST21
Topological domaini143 – 148Cytoplasmic1 Publication6
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 198Periplasmic1 PublicationAdd BLAST29
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21
Topological domaini220 – 270Cytoplasmic1 PublicationAdd BLAST51
Transmembranei271 – 291HelicalSequence analysisAdd BLAST21
Topological domaini292 – 300Periplasmic1 Publication9
Transmembranei301 – 321HelicalSequence analysisAdd BLAST21
Topological domaini322 – 354Cytoplasmic1 PublicationAdd BLAST33

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000601911 – 354Glycine betaine/proline betaine transport system permease protein ProWAdd BLAST354

Proteomic databases

PaxDbiP14176.
PRIDEiP14176.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ProV), two transmembrane proteins (ProW) and a solute-binding protein (ProX).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
proVP141752EBI-8794761,EBI-546797

Protein-protein interaction databases

BioGridi4259449. 24 interactors.
IntActiP14176. 1 interactor.
STRINGi511145.b2678.

Structurei

3D structure databases

ProteinModelPortaliP14176.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini145 – 324ABC transmembrane type-1PROSITE-ProRule annotationAdd BLAST180

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108HQS. Bacteria.
COG4176. LUCA.
HOGENOMiHOG000279300.
InParanoidiP14176.
KOiK02001.
OMAiHFNIMDP.
PhylomeDBiP14176.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQNNPWDT TPAADSAAQS ADAWGTPTTA PTDGGGADWL TSTPAPNVEH
60 70 80 90 100
FNILDPFHKT LIPLDSWVTE GIDWVVTHFR PVFQGVRVPV DYILNGFQQL
110 120 130 140 150
LLGMPAPVAI IVFALIAWQI SGVGMGVATL VSLIAIGAIG AWSQAMVTLA
160 170 180 190 200
LVLTALLFCI VIGLPLGIWL ARSPRAAKII RPLLDAMQTT PAFVYLVPIV
210 220 230 240 250
MLFGIGNVPG VVVTIIFALP PIIRLTILGI NQVPADLIEA SRSFGASPRQ
260 270 280 290 300
MLFKVQLPLA MPTIMAGVNQ TLMLALSMVV IASMIAVGGL GQMVLRGIGR
310 320 330 340 350
LDMGLATVGG VGIVILAIIL DRLTQAVGRD SRSRGNRRWY TTGPVGLLTR

PFIK
Length:354
Mass (Da):37,620
Last modified:January 1, 1990 - v1
Checksum:iD35F94A74E2779D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24428.1.
U00096 Genomic DNA. Translation: AAC75725.1.
AP009048 Genomic DNA. Translation: BAA16543.1.
PIRiJS0129. MMECPW.
RefSeqiNP_417164.1. NC_000913.3.
WP_000774988.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75725; AAC75725; b2678.
BAA16543; BAA16543; BAA16543.
GeneIDi947145.
KEGGiecj:JW2653.
eco:b2678.
PATRICi32120746. VBIEscCol129921_2770.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24428.1.
U00096 Genomic DNA. Translation: AAC75725.1.
AP009048 Genomic DNA. Translation: BAA16543.1.
PIRiJS0129. MMECPW.
RefSeqiNP_417164.1. NC_000913.3.
WP_000774988.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP14176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259449. 24 interactors.
IntActiP14176. 1 interactor.
STRINGi511145.b2678.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP14176.
PRIDEiP14176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75725; AAC75725; b2678.
BAA16543; BAA16543; BAA16543.
GeneIDi947145.
KEGGiecj:JW2653.
eco:b2678.
PATRICi32120746. VBIEscCol129921_2770.

Organism-specific databases

EchoBASEiEB0765.
EcoGeneiEG10772. proW.

Phylogenomic databases

eggNOGiENOG4108HQS. Bacteria.
COG4176. LUCA.
HOGENOMiHOG000279300.
InParanoidiP14176.
KOiK02001.
OMAiHFNIMDP.
PhylomeDBiP14176.

Enzyme and pathway databases

BioCyciEcoCyc:PROW-MONOMER.
ECOL316407:JW2653-MONOMER.
MetaCyc:PROW-MONOMER.

Miscellaneous databases

PROiP14176.

Family and domain databases

CDDicd06261. TM_PBP2. 1 hit.
Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPROW_ECOLI
AccessioniPrimary (citable) accession number: P14176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: November 2, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.