Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P14176 (PROW_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycine betaine/L-proline transport system permease protein ProW
Gene names
Name:proW
Ordered Locus Names:b2678, JW2653
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline.

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.

Contains 1 ABC transmembrane type-1 domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

proVP141752EBI-8794761,EBI-546797

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Glycine betaine/L-proline transport system permease protein ProW
PRO_0000060191

Regions

Topological domain1 – 9999Cytoplasmic Potential
Transmembrane100 – 12021Helical; Potential
Topological domain1211Periplasmic Potential
Transmembrane122 – 14221Helical; Potential
Topological domain143 – 1508Cytoplasmic Potential
Transmembrane151 – 17121Helical; Potential
Topological domain172 – 19827Periplasmic Potential
Transmembrane199 – 21921Helical; Potential
Topological domain220 – 27051Cytoplasmic Potential
Transmembrane271 – 29121Helical; Potential
Topological domain292 – 3009Periplasmic Potential
Transmembrane301 – 32121Helical; Potential
Topological domain322 – 35433Cytoplasmic Potential
Domain145 – 324180ABC transmembrane type-1

Sequences

Sequence LengthMass (Da)Tools
P14176 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: D35F94A74E2779D1

FASTA35437,620
        10         20         30         40         50         60 
MADQNNPWDT TPAADSAAQS ADAWGTPTTA PTDGGGADWL TSTPAPNVEH FNILDPFHKT 

        70         80         90        100        110        120 
LIPLDSWVTE GIDWVVTHFR PVFQGVRVPV DYILNGFQQL LLGMPAPVAI IVFALIAWQI 

       130        140        150        160        170        180 
SGVGMGVATL VSLIAIGAIG AWSQAMVTLA LVLTALLFCI VIGLPLGIWL ARSPRAAKII 

       190        200        210        220        230        240 
RPLLDAMQTT PAFVYLVPIV MLFGIGNVPG VVVTIIFALP PIIRLTILGI NQVPADLIEA 

       250        260        270        280        290        300 
SRSFGASPRQ MLFKVQLPLA MPTIMAGVNQ TLMLALSMVV IASMIAVGGL GQMVLRGIGR 

       310        320        330        340        350 
LDMGLATVGG VGIVILAIIL DRLTQAVGRD SRSRGNRRWY TTGPVGLLTR PFIK 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli."
Gowrishankar J.
J. Bacteriol. 171:1923-1931(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]Erratum
Gowrishankar J.
J. Bacteriol. 172:1165-1165(1990)
[3]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24856 Genomic DNA. Translation: AAA24428.1.
U00096 Genomic DNA. Translation: AAC75725.1.
AP009048 Genomic DNA. Translation: BAA16543.1.
PIRMMECPW. JS0129.
RefSeqNP_417164.1. NC_000913.3.
YP_490893.1. NC_007779.1.

3D structure databases

ProteinModelPortalP14176.
SMRP14176. Positions 148-273.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP14176. 1 interaction.
STRING511145.b2678.

Protein family/group databases

TCDB3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75725; AAC75725; b2678.
BAA16543; BAA16543; BAA16543.
GeneID12930455.
947145.
KEGGecj:Y75_p2621.
eco:b2678.
PATRIC32120746. VBIEscCol129921_2770.

Organism-specific databases

EchoBASEEB0765.
EcoGeneEG10772. proW.

Phylogenomic databases

eggNOGCOG4176.
HOGENOMHOG000279300.
KOK02001.
OMAHFNIMDP.
OrthoDBEOG6H4KBG.
PhylomeDBP14176.

Enzyme and pathway databases

BioCycEcoCyc:PROW-MONOMER.
ECOL316407:JW2653-MONOMER.
MetaCyc:PROW-MONOMER.
RETL1328306-WGS:GSTH-4736-MONOMER.

Gene expression databases

GenevestigatorP14176.

Family and domain databases

Gene3D1.10.3720.10. 1 hit.
InterProIPR000515. MetI-like.
[Graphical view]
PfamPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMSSF161098. SSF161098. 1 hit.
PROSITEPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP14176.

Entry information

Entry namePROW_ECOLI
AccessionPrimary (citable) accession number: P14176
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: July 9, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene