Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P14176

- PROW_ECOLI

UniProt

P14176 - PROW_ECOLI

Protein

Glycine betaine/L-proline transport system permease protein ProW

Gene

proW

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in a multicomponent binding-protein-dependent transport system for glycine betaine/L-proline.

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transporter activity Source: EcoCyc

    GO - Biological processi

    1. amino acid transport Source: UniProtKB-KW
    2. response to osmotic stress Source: EcoCyc

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:PROW-MONOMER.
    ECOL316407:JW2653-MONOMER.
    MetaCyc:PROW-MONOMER.
    RETL1328306-WGS:GSTH-4736-MONOMER.

    Protein family/group databases

    TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycine betaine/L-proline transport system permease protein ProW
    Gene namesi
    Name:proW
    Ordered Locus Names:b2678, JW2653
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10772. proW.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 354354Glycine betaine/L-proline transport system permease protein ProWPRO_0000060191Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP14176.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    proVP141752EBI-8794761,EBI-546797

    Protein-protein interaction databases

    IntActiP14176. 1 interaction.
    STRINGi511145.b2678.

    Structurei

    3D structure databases

    ProteinModelPortaliP14176.
    SMRiP14176. Positions 148-273.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9999CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini121 – 1211PeriplasmicSequence Analysis
    Topological domaini143 – 1508CytoplasmicSequence Analysis
    Topological domaini172 – 19827PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini220 – 27051CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini292 – 3009PeriplasmicSequence Analysis
    Topological domaini322 – 35433CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei100 – 12021HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei122 – 14221HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei151 – 17121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei199 – 21921HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei271 – 29121HelicalPROSITE-ProRule annotationAdd
    BLAST
    Transmembranei301 – 32121HelicalPROSITE-ProRule annotationAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini145 – 324180ABC transmembrane type-1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4176.
    HOGENOMiHOG000279300.
    KOiK02001.
    OMAiHFNIMDP.
    OrthoDBiEOG6H4KBG.
    PhylomeDBiP14176.

    Family and domain databases

    Gene3Di1.10.3720.10. 1 hit.
    InterProiIPR000515. MetI-like.
    [Graphical view]
    PfamiPF00528. BPD_transp_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF161098. SSF161098. 1 hit.
    PROSITEiPS50928. ABC_TM1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P14176-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADQNNPWDT TPAADSAAQS ADAWGTPTTA PTDGGGADWL TSTPAPNVEH    50
    FNILDPFHKT LIPLDSWVTE GIDWVVTHFR PVFQGVRVPV DYILNGFQQL 100
    LLGMPAPVAI IVFALIAWQI SGVGMGVATL VSLIAIGAIG AWSQAMVTLA 150
    LVLTALLFCI VIGLPLGIWL ARSPRAAKII RPLLDAMQTT PAFVYLVPIV 200
    MLFGIGNVPG VVVTIIFALP PIIRLTILGI NQVPADLIEA SRSFGASPRQ 250
    MLFKVQLPLA MPTIMAGVNQ TLMLALSMVV IASMIAVGGL GQMVLRGIGR 300
    LDMGLATVGG VGIVILAIIL DRLTQAVGRD SRSRGNRRWY TTGPVGLLTR 350
    PFIK 354
    Length:354
    Mass (Da):37,620
    Last modified:January 1, 1990 - v1
    Checksum:iD35F94A74E2779D1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24856 Genomic DNA. Translation: AAA24428.1.
    U00096 Genomic DNA. Translation: AAC75725.1.
    AP009048 Genomic DNA. Translation: BAA16543.1.
    PIRiJS0129. MMECPW.
    RefSeqiNP_417164.1. NC_000913.3.
    YP_490893.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75725; AAC75725; b2678.
    BAA16543; BAA16543; BAA16543.
    GeneIDi12930455.
    947145.
    KEGGiecj:Y75_p2621.
    eco:b2678.
    PATRICi32120746. VBIEscCol129921_2770.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M24856 Genomic DNA. Translation: AAA24428.1 .
    U00096 Genomic DNA. Translation: AAC75725.1 .
    AP009048 Genomic DNA. Translation: BAA16543.1 .
    PIRi JS0129. MMECPW.
    RefSeqi NP_417164.1. NC_000913.3.
    YP_490893.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P14176.
    SMRi P14176. Positions 148-273.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P14176. 1 interaction.
    STRINGi 511145.b2678.

    Protein family/group databases

    TCDBi 3.A.1.12.1. the atp-binding cassette (abc) superfamily.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75725 ; AAC75725 ; b2678 .
    BAA16543 ; BAA16543 ; BAA16543 .
    GeneIDi 12930455.
    947145.
    KEGGi ecj:Y75_p2621.
    eco:b2678.
    PATRICi 32120746. VBIEscCol129921_2770.

    Organism-specific databases

    EchoBASEi EB0765.
    EcoGenei EG10772. proW.

    Phylogenomic databases

    eggNOGi COG4176.
    HOGENOMi HOG000279300.
    KOi K02001.
    OMAi HFNIMDP.
    OrthoDBi EOG6H4KBG.
    PhylomeDBi P14176.

    Enzyme and pathway databases

    BioCyci EcoCyc:PROW-MONOMER.
    ECOL316407:JW2653-MONOMER.
    MetaCyc:PROW-MONOMER.
    RETL1328306-WGS:GSTH-4736-MONOMER.

    Miscellaneous databases

    PROi P14176.

    Gene expression databases

    Genevestigatori P14176.

    Family and domain databases

    Gene3Di 1.10.3720.10. 1 hit.
    InterProi IPR000515. MetI-like.
    [Graphical view ]
    Pfami PF00528. BPD_transp_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF161098. SSF161098. 1 hit.
    PROSITEi PS50928. ABC_TM1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli."
      Gowrishankar J.
      J. Bacteriol. 171:1923-1931(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Erratum
      Gowrishankar J.
      J. Bacteriol. 172:1165-1165(1990)
    3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiPROW_ECOLI
    AccessioniPrimary (citable) accession number: P14176
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3