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Protein

Glycine betaine/proline betaine transport system ATP-binding protein ProV

Gene

proV

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ProU ABC transporter complex involved in glycine betaine and proline betaine uptake (PubMed:3305496, PubMed:7898450, PubMed:23249124). Probably responsible for energy coupling to the transport system (Probable).Curated3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi61 – 688ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • amino acid transmembrane transporter activity Source: EcoCyc
  • ATPase activity Source: InterPro
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • amino acid transmembrane transport Source: GOC
  • glycine betaine transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:PROV-MONOMER.
ECOL316407:JW2652-MONOMER.
MetaCyc:PROV-MONOMER.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine/proline betaine transport system ATP-binding protein ProVCurated
Gene namesi
Name:proV1 Publication
Ordered Locus Names:b2677, JW2652
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10771. proV.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 400400Glycine betaine/proline betaine transport system ATP-binding protein ProVPRO_0000092761Add
BLAST

Proteomic databases

PaxDbiP14175.
PRIDEiP14175.

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (ProV), two transmembrane proteins (ProW) and a solute-binding protein (ProX).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
proWP141762EBI-546797,EBI-8794761

Protein-protein interaction databases

BioGridi4262264. 16 interactions.
DIPiDIP-10574N.
IntActiP14175. 6 interactions.
MINTiMINT-1249790.
STRINGi511145.b2677.

Structurei

3D structure databases

ProteinModelPortaliP14175.
SMRiP14175. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 265237ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini282 – 34160CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini343 – 40058CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transporter domain.PROSITE-ProRule annotation
Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

eggNOGiENOG4108IJ7. Bacteria.
COG4175. LUCA.
InParanoidiP14175.
KOiK02000.
OMAiPLIRLEM.
OrthoDBiEOG6T7N3V.
PhylomeDBiP14175.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIKLEIKNL YKIFGEHPQR AFKYIEQGLS KEQILEKTGL SLGVKDASLA
60 70 80 90 100
IEEGEIFVIM GLSGSGKSTM VRLLNRLIEP TRGQVLIDGV DIAKISDAEL
110 120 130 140 150
REVRRKKIAM VFQSFALMPH MTVLDNTAFG MELAGINAEE RREKALDALR
160 170 180 190 200
QVGLENYAHS YPDELSGGMR QRVGLARALA INPDILLMDE AFSALDPLIR
210 220 230 240 250
TEMQDELVKL QAKHQRTIVF ISHDLDEAMR IGDRIAIMQN GEVVQVGTPD
260 270 280 290 300
EILNNPANDY VRTFFRGVDI SQVFSAKDIA RRTPNGLIRK TPGFGPRSAL
310 320 330 340 350
KLLQDEDREY GYVIERGNKF VGAVSIDSLK TALTQQQGLD AALIDAPLAV
360 370 380 390 400
DAQTPLSELL SHVGQAPCAV PVVDEDQQYV GIISKGMLLR ALDREGVNNG
Length:400
Mass (Da):44,163
Last modified:January 1, 1990 - v1
Checksum:i45A98D45F028B1B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24427.1.
U00096 Genomic DNA. Translation: AAC75724.1.
AP009048 Genomic DNA. Translation: BAA16542.1.
X52694 Genomic DNA. Translation: CAA36923.1.
PIRiJS0128. BVECPV.
RefSeqiNP_417163.1. NC_000913.3.
WP_000985494.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75724; AAC75724; b2677.
BAA16542; BAA16542; BAA16542.
GeneIDi947148.
KEGGiecj:JW2652.
eco:b2677.
PATRICi32120744. VBIEscCol129921_2769.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M24856 Genomic DNA. Translation: AAA24427.1.
U00096 Genomic DNA. Translation: AAC75724.1.
AP009048 Genomic DNA. Translation: BAA16542.1.
X52694 Genomic DNA. Translation: CAA36923.1.
PIRiJS0128. BVECPV.
RefSeqiNP_417163.1. NC_000913.3.
WP_000985494.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP14175.
SMRiP14175. Positions 1-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262264. 16 interactions.
DIPiDIP-10574N.
IntActiP14175. 6 interactions.
MINTiMINT-1249790.
STRINGi511145.b2677.

Protein family/group databases

TCDBi3.A.1.12.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP14175.
PRIDEiP14175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75724; AAC75724; b2677.
BAA16542; BAA16542; BAA16542.
GeneIDi947148.
KEGGiecj:JW2652.
eco:b2677.
PATRICi32120744. VBIEscCol129921_2769.

Organism-specific databases

EchoBASEiEB0764.
EcoGeneiEG10771. proV.

Phylogenomic databases

eggNOGiENOG4108IJ7. Bacteria.
COG4175. LUCA.
InParanoidiP14175.
KOiK02000.
OMAiPLIRLEM.
OrthoDBiEOG6T7N3V.
PhylomeDBiP14175.

Enzyme and pathway databases

BioCyciEcoCyc:PROV-MONOMER.
ECOL316407:JW2652-MONOMER.
MetaCyc:PROV-MONOMER.

Miscellaneous databases

PROiP14175.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli."
    Gowrishankar J.
    J. Bacteriol. 171:1923-1931(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Erratum
    Gowrishankar J.
    J. Bacteriol. 172:1165-1165(1990)
  3. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Molecular characterization of the proU loci of Salmonella typhimurium and Escherichia coli encoding osmoregulated glycine betaine transport systems."
    Stirling D.A., Hulton C.S.J., Waddell L., Park S.F., Stewart G.S.A.B., Booth I.R., Higgins C.F.
    Mol. Microbiol. 3:1025-1038(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-224.
    Strain: K12.
  7. "Purification and characterization of a glycine betaine binding protein from Escherichia coli."
    Barron A., Jung J.U., Villarejo W.
    J. Biol. Chem. 262:11841-11846(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCINE BETAINE TRANSPORT.
  8. "The osmoprotectant proline betaine is a major substrate for the binding-protein-dependent transport system ProU of Escherichia coli K-12."
    Haardt M., Kempf B., Faatz E., Bremer E.
    Mol. Gen. Genet. 246:783-786(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PROLINE BETAINE TRANSPORT.
  9. "Escherichia coli proteome analysis using the gene-protein database."
    VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
    Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY 2D-GEL.
  10. Cited for: SUBCELLULAR LOCATION.
    Strain: BL21-DE3.
  11. "Functional reconstitution and osmoregulatory properties of the ProU ABC transporter from Escherichia coli."
    Gul N., Poolman B.
    Mol. Membr. Biol. 30:138-148(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCINE BETAINE TRANSPORT, SUBUNIT.
    Strain: K12.

Entry informationi

Entry nameiPROV_ECOLI
AccessioniPrimary (citable) accession number: P14175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 11, 2016
This is version 154 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.