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P14171 (BLAC_RHOCA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase

EC=3.5.2.6
Alternative name(s):
Penicillinase
OrganismRhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifier1061 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeRhodobacter

Protein attributes

Sequence length293 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Hydrolyzes beta-lactams antibiotics. Rates of hydrolysis relative to benzylpenicillin =100: ampicillin = 27, carbenicillin = 25, cloxacillin = 0, cephaloridine = 4.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   Cellular componentPeriplasm
   DomainSignal
   Molecular functionHydrolase
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 293266Beta-lactamase
PRO_0000017009

Regions

Region238 – 2403Substrate binding By similarity

Sites

Active site741Acyl-ester intermediate By similarity

Sequences

Sequence LengthMass (Da)Tools
P14171 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: 60925BBE658917F1

FASTA29331,296
        10         20         30         40         50         60 
MRFTATVLSR VATGLALGLS MATASLAETP VEALSETVAR IEEQLGARVG LSLMETGTGW 

        70         80         90        100        110        120 
SWSHREDELF LMNSTVKVPV CGAILARWDA GRLSLSDALP VRKADLVPYA PVTETRVGGN 

       130        140        150        160        170        180 
MTLDELCLAA IDMSDNVAAN ILIGHLGGPE AVTQFFRSVG DPTSRLDRIE PKLNDFASGD 

       190        200        210        220        230        240 
ERDTTSPAAM SETLRALLLG DVLSPEARGK LAEWMRHGGV TGALLRAEAE DAWLILDKSG 

       250        260        270        280        290 
SGSHTRNLVA VIQPEGGAPW IATMFISDTD AEFEVRNEAL KDLGRAVVAV VRE 

« Hide

References

[1]"The phototrophic bacterium Rhodopseudomonas capsulata sp108 encodes an indigenous class A beta-lactamase."
Campbell J.I.A., Scahill S., Gibson T., Ambler R.P.
Biochem. J. 260:803-812(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SP108.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15791 Genomic DNA. Translation: CAA33795.1.
PIRS04649.

3D structure databases

ProteinModelPortalP14171.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PfamPF00144. Beta-lactamase. 1 hit.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLAC_RHOCA
AccessionPrimary (citable) accession number: P14171
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 16, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families