Reviewed, UniProtKB/Swiss-Prot P14171 (BLAC_RHOCA)

Names and origin

Protein names	Recommended name:     Beta-lactamase     EC=3.5.2.6 Alternative name(s):     Penicillinase
Organism	Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Taxonomic identifier	1061 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	293 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes beta-lactams antibiotics. Rates of hydrolysis relative to benzylpenicillin =100: ampicillin = 27, carbenicillin = 25, cloxacillin = 0, cephaloridine = 4.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 293	266	Beta-lactamase		PRO_0000017009
Regions
	Region	238 – 240	3	Substrate binding By similarity
Sites
	Active site	74	1	Acyl-ester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P14171-1 [UniParc]. Last modified January 1, 1990. Version 1. Checksum: 60925BBE658917F1	FASTA	293	31,296
	10 20 30 40 50 60 MRFTATVLSR VATGLALGLS MATASLAETP VEALSETVAR IEEQLGARVG LSLMETGTGW 70 80 90 100 110 120 SWSHREDELF LMNSTVKVPV CGAILARWDA GRLSLSDALP VRKADLVPYA PVTETRVGGN 130 140 150 160 170 180 MTLDELCLAA IDMSDNVAAN ILIGHLGGPE AVTQFFRSVG DPTSRLDRIE PKLNDFASGD 190 200 210 220 230 240 ERDTTSPAAM SETLRALLLG DVLSPEARGK LAEWMRHGGV TGALLRAEAE DAWLILDKSG 250 260 270 280 290 SGSHTRNLVA VIQPEGGAPW IATMFISDTD AEFEVRNEAL KDLGRAVVAV VRE

References

[1]	"The phototrophic bacterium Rhodopseudomonas capsulata sp108 encodes an indigenous class A beta-lactamase." Campbell J.I.A., Scahill S., Gibson T., Ambler R.P. Biochem. J. 260:803-812(1989) [PubMed: 2788410] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SP108.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15791 Genomic DNA. Translation: CAA33795.1.
PIR	S04649.
3D structure databases
HSSP	HSSP built from PDB template 1TEM based on UniProtKB P00810.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 567.
Family and domain databases
InterPro	IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAC_RHOCA

Accession

Primary (citable) accession number: P14171

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 1, 1990
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)