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Protein

Osmotin

Gene

AP24

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Pathogenesis-related protein

Keywords - Biological processi

Plant defense, Stress response

Names & Taxonomyi

Protein namesi
Recommended name:
Osmotin
Gene namesi
Name:AP24
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

  • Vacuole

  • Note: Vacuolar inclusion body.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

Pathology & Biotechi

Protein family/group databases

Allergomei9618. Nic t Osmotin.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 211 PublicationAdd BLAST21
ChainiPRO_000003404322 – 246OsmotinAdd BLAST225

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 225
Disulfide bondi72 ↔ 82
Disulfide bondi87 ↔ 93
Disulfide bondi141 ↔ 213
Disulfide bondi146 ↔ 196
Disulfide bondi154 ↔ 164
Disulfide bondi168 ↔ 177
Disulfide bondi178 ↔ 183

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP14170.

Expressioni

Tissue specificityi

Highly expressed in roots and flower buds.1 Publication

Inductioni

By salt stress, abscisic acid (ABA), viral infection and wounding.1 Publication

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi23 – 28Combined sources6
Beta strandi30 – 32Combined sources3
Beta strandi34 – 39Combined sources6
Turni40 – 42Combined sources3
Beta strandi43 – 47Combined sources5
Beta strandi52 – 56Combined sources5
Beta strandi63 – 74Combined sources12
Beta strandi78 – 85Combined sources8
Beta strandi88 – 92Combined sources5
Beta strandi103 – 108Combined sources6
Turni110 – 112Combined sources3
Beta strandi113 – 120Combined sources8
Beta strandi125 – 127Combined sources3
Beta strandi129 – 135Combined sources7
Beta strandi144 – 146Combined sources3
Helixi150 – 153Combined sources4
Turni156 – 158Combined sources3
Helixi167 – 171Combined sources5
Helixi174 – 177Combined sources4
Helixi187 – 195Combined sources9
Helixi206 – 209Combined sources4
Beta strandi211 – 214Combined sources4
Beta strandi220 – 224Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PCVX-ray2.30A/B22-226[»]
ProteinModelPortaliP14170.
SMRiP14170.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14170.

Family & Domainsi

Sequence similaritiesi

Belongs to the thaumatin family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14170-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNLRSSFVF FLLALVTYTY AATIEVRNNC PYTVWAASTP IGGGRRLDRG
60 70 80 90 100
QTWVINAPRG TKMARVWGRT NCNFNAAGRG TCQTGDCGGV LQCTGWGKPP
110 120 130 140 150
NTLAEYALDQ FSGLDFWDIS LVDGFNIPMT FAPTNPSGGK CHAIHCTANI
160 170 180 190 200
NGECPRELRV PGGCNNPCTT FGGQQYCCTQ GPCGPTFFSK FFKQRCPDAY
210 220 230 240
SYPQDDPTST FTCPGGSTNY RVIFCPNGQA HPNFPLEMPG SDEVAK
Length:246
Mass (Da):26,681
Last modified:October 1, 1996 - v2
Checksum:i3CDBA991ACA2B0B1
GO

Sequence cautioni

The sequence CAA46623 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2 – 3GN → EY in CAA64620 (Ref. 5) Curated2
Sequence conflicti15L → F in CAA64620 (Ref. 5) Curated1
Sequence conflicti18Y → C in CAA64620 (Ref. 5) Curated1
Sequence conflicti62K → N in CAA43854 (PubMed:1536937).Curated1
Sequence conflicti66V → I in CAA64620 (Ref. 5) Curated1
Sequence conflicti122V → L in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti129M → I in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti132Missing in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti144 – 145IH → L in AAA34089 (PubMed:16666857).Curated2
Sequence conflicti149Missing in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti151 – 155NGECP → RRMS in CAA43854 (PubMed:1536937).Curated5
Sequence conflicti156R → A in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti181G → R in AAA34089 (PubMed:16666857).Curated1
Sequence conflicti221R → K in CAA64620 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65700 mRNA. Translation: CAA46622.1.
X65701 Genomic DNA. Translation: CAA46623.1. Different initiation.
S40046 Genomic DNA. Translation: AAB22459.2.
M29279 mRNA. Translation: AAA34089.1.
X61679 mRNA. Translation: CAA43854.1.
X95308 Genomic DNA. Translation: CAA64620.1.
S44889 mRNA. Translation: AAB23375.1.
PIRiS30157.
S34794.
RefSeqiNP_001312374.1. NM_001325445.1.
XP_016464912.1. XM_016609426.1.
UniGeneiNta.2852.
Nta.5214.

Genome annotation databases

GeneIDi107787819.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65700 mRNA. Translation: CAA46622.1.
X65701 Genomic DNA. Translation: CAA46623.1. Different initiation.
S40046 Genomic DNA. Translation: AAB22459.2.
M29279 mRNA. Translation: AAA34089.1.
X61679 mRNA. Translation: CAA43854.1.
X95308 Genomic DNA. Translation: CAA64620.1.
S44889 mRNA. Translation: AAB23375.1.
PIRiS30157.
S34794.
RefSeqiNP_001312374.1. NM_001325445.1.
XP_016464912.1. XM_016609426.1.
UniGeneiNta.2852.
Nta.5214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PCVX-ray2.30A/B22-226[»]
ProteinModelPortaliP14170.
SMRiP14170.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei9618. Nic t Osmotin.

Proteomic databases

PRIDEiP14170.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107787819.

Miscellaneous databases

EvolutionaryTraceiP14170.

Family and domain databases

Gene3Di2.60.110.10. 1 hit.
InterProiIPR001938. Thaumatin.
IPR017949. Thaumatin_CS.
[Graphical view]
PANTHERiPTHR31048. PTHR31048. 1 hit.
PfamiPF00314. Thaumatin. 1 hit.
[Graphical view]
PIRSFiPIRSF002703. Thaumatin. 1 hit.
PRINTSiPR00347. THAUMATIN.
SMARTiSM00205. THN. 1 hit.
[Graphical view]
SUPFAMiSSF49870. SSF49870. 1 hit.
PROSITEiPS00316. THAUMATIN_1. 1 hit.
PS51367. THAUMATIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOSMO_TOBAC
AccessioniPrimary (citable) accession number: P14170
Secondary accession number(s): Q40529, Q6LDD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibits the germination and growth of the fungus Phytophthora infestans.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.