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Protein

CDP-paratose 2-epimerase

Gene

rfbE

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomeration of CDP-paratose to CDP-tyvelose.

Catalytic activityi

CDP-3,6-dideoxy-D-glucose = CDP-3,6-dideoxy-D-mannose.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: CDP-3,6-dideoxy-D-mannose biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glucose-1-phosphate cytidylyltransferase (rfbF)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. CDP-paratose synthase (rfbS)
  5. CDP-paratose 2-epimerase (rfbE)
This subpathway is part of the pathway CDP-3,6-dideoxy-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate, the pathway CDP-3,6-dideoxy-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124Substrate1 Publication1
Active sitei164Proton acceptor1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13795.
UniPathwayiUPA00055; UER00515.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-paratose 2-epimerase (EC:5.1.3.10)
Alternative name(s):
CDP-tyvelose 2-epimerase
Gene namesi
Name:rfbE
Ordered Locus Names:STY2298, t0784
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832601 – 338CDP-paratose 2-epimeraseAdd BLAST338

Proteomic databases

PRIDEiP14169.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi220341.STY2298.

Structurei

Secondary structure

1338
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Turni7 – 9Combined sources3
Helixi11 – 22Combined sources12
Beta strandi26 – 31Combined sources6
Helixi38 – 46Combined sources9
Beta strandi52 – 55Combined sources4
Helixi61 – 71Combined sources11
Beta strandi74 – 78Combined sources5
Helixi85 – 90Combined sources6
Helixi92 – 113Combined sources22
Beta strandi118 – 124Combined sources7
Helixi125 – 128Combined sources4
Beta strandi136 – 138Combined sources3
Beta strandi143 – 145Combined sources3
Helixi162 – 182Combined sources21
Beta strandi185 – 191Combined sources7
Helixi207 – 219Combined sources13
Beta strandi226 – 232Combined sources7
Beta strandi235 – 237Combined sources3
Helixi241 – 253Combined sources13
Helixi255 – 258Combined sources4
Beta strandi262 – 267Combined sources6
Helixi269 – 271Combined sources3
Beta strandi272 – 274Combined sources3
Helixi275 – 286Combined sources12
Beta strandi292 – 295Combined sources4
Beta strandi303 – 305Combined sources3
Helixi310 – 316Combined sources7
Helixi324 – 337Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORRX-ray1.50A/B/C/D2-338[»]
ProteinModelPortaliP14169.
SMRiP14169.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14169.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IAJ. Bacteria.
COG0451. LUCA.
HOGENOMiHOG000167994.
KOiK12454.
OMAiHERRTIT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P14169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLITGGCG FLGSNLASFA LSQGIDLIVF DNLSRKGATD NLHWLSSLGN
60 70 80 90 100
FEFVHGDIRN KNDVTRLITK YMPDSCFHLA GQVAMTTSID NPCMDFEINV
110 120 130 140 150
GGTLNLLEAV RQYNSNCNII YSSTNKVYGD LEQYKYNETE TRYTCVDKPN
160 170 180 190 200
GYDESTQLDF HSPYGCSKGA ADQYMLDYAR IFGLNTVVFR HSSMYGGRQF
210 220 230 240 250
ATYDQGWVGW FCQKAVEIKN GINKPFTISG NGKQVRDVLH AEDMISLYFT
260 270 280 290 300
ALANVSKIRG NAFNIGGTIV NSLSLLELFK LLEDYCNIDM RFTNLPVRES
310 320 330
DQRVFVADIK KITNAIDWSP KVSAKDGVQK MYDWTSSI
Length:338
Mass (Da):37,958
Last modified:February 1, 1996 - v2
Checksum:i031175EDCF533888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29682 Genomic DNA. Translation: AAB49384.1.
AL513382 Genomic DNA. Translation: CAD02451.1.
AE014613 Genomic DNA. Translation: AAO68475.1.
PIRiB33604.
RefSeqiNP_456637.1. NC_003198.1.
WP_000770936.1. NZ_LUHL01000038.1.

Genome annotation databases

EnsemblBacteriaiAAO68475; AAO68475; t0784.
CAD02451; CAD02451; CAD02451.
GeneIDi1248633.
KEGGistt:t0784.
sty:STY2298.
PATRICi18542537. VBISalEnt120419_2318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29682 Genomic DNA. Translation: AAB49384.1.
AL513382 Genomic DNA. Translation: CAD02451.1.
AE014613 Genomic DNA. Translation: AAO68475.1.
PIRiB33604.
RefSeqiNP_456637.1. NC_003198.1.
WP_000770936.1. NZ_LUHL01000038.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ORRX-ray1.50A/B/C/D2-338[»]
ProteinModelPortaliP14169.
SMRiP14169.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY2298.

Proteomic databases

PRIDEiP14169.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO68475; AAO68475; t0784.
CAD02451; CAD02451; CAD02451.
GeneIDi1248633.
KEGGistt:t0784.
sty:STY2298.
PATRICi18542537. VBISalEnt120419_2318.

Phylogenomic databases

eggNOGiENOG4108IAJ. Bacteria.
COG0451. LUCA.
HOGENOMiHOG000167994.
KOiK12454.
OMAiHERRTIT.

Enzyme and pathway databases

UniPathwayiUPA00055; UER00515.
BioCyciMetaCyc:MONOMER-13795.

Miscellaneous databases

EvolutionaryTraceiP14169.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRFBE_SALTI
AccessioniPrimary (citable) accession number: P14169
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.