Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CDP-paratose 2-epimerase

Gene

rfbE

Organism
Salmonella typhi
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the isomeration of CDP-paratose to CDP-tyvelose.

Catalytic activityi

CDP-3,6-dideoxy-D-glucose = CDP-3,6-dideoxy-D-mannose.

Cofactori

NAD+Note: Binds 1 NAD+ per subunit.

Pathwayi: CDP-3,6-dideoxy-D-mannose biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glucose-1-phosphate cytidylyltransferase (rfbF)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. CDP-paratose synthase (rfbS)
  5. CDP-paratose 2-epimerase (rfbE)
This subpathway is part of the pathway CDP-3,6-dideoxy-D-mannose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CDP-3,6-dideoxy-D-mannose from CTP and alpha-D-glucose 1-phosphate, the pathway CDP-3,6-dideoxy-D-mannose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei124 – 1241Substrate1 Publication
Active sitei164 – 1641Proton acceptor

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13795.
UniPathwayiUPA00055; UER00515.

Names & Taxonomyi

Protein namesi
Recommended name:
CDP-paratose 2-epimerase (EC:5.1.3.10)
Alternative name(s):
CDP-tyvelose 2-epimerase
Gene namesi
Name:rfbE
Ordered Locus Names:STY2298, t0784
OrganismiSalmonella typhi
Taxonomic identifieri90370 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000002670 Componenti: Chromosome
  • UP000000541 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338CDP-paratose 2-epimerasePRO_0000183260Add
BLAST

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi220341.STY2298.

Structurei

Secondary structure

1
338
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 65Combined sources
Turni7 – 93Combined sources
Helixi11 – 2212Combined sources
Beta strandi26 – 316Combined sources
Helixi38 – 469Combined sources
Beta strandi52 – 554Combined sources
Helixi61 – 7111Combined sources
Beta strandi74 – 785Combined sources
Helixi85 – 906Combined sources
Helixi92 – 11322Combined sources
Beta strandi118 – 1247Combined sources
Helixi125 – 1284Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi143 – 1453Combined sources
Helixi162 – 18221Combined sources
Beta strandi185 – 1917Combined sources
Helixi207 – 21913Combined sources
Beta strandi226 – 2327Combined sources
Beta strandi235 – 2373Combined sources
Helixi241 – 25313Combined sources
Helixi255 – 2584Combined sources
Beta strandi262 – 2676Combined sources
Helixi269 – 2713Combined sources
Beta strandi272 – 2743Combined sources
Helixi275 – 28612Combined sources
Beta strandi292 – 2954Combined sources
Beta strandi303 – 3053Combined sources
Helixi310 – 3167Combined sources
Helixi324 – 33714Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ORRX-ray1.50A/B/C/D2-338[»]
ProteinModelPortaliP14169.
SMRiP14169. Positions 2-338.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP14169.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IAJ. Bacteria.
COG0451. LUCA.
HOGENOMiHOG000167994.
KOiK12454.
OMAiDFANTIR.
OrthoDBiEOG6TXQT1.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P14169-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLITGGCG FLGSNLASFA LSQGIDLIVF DNLSRKGATD NLHWLSSLGN
60 70 80 90 100
FEFVHGDIRN KNDVTRLITK YMPDSCFHLA GQVAMTTSID NPCMDFEINV
110 120 130 140 150
GGTLNLLEAV RQYNSNCNII YSSTNKVYGD LEQYKYNETE TRYTCVDKPN
160 170 180 190 200
GYDESTQLDF HSPYGCSKGA ADQYMLDYAR IFGLNTVVFR HSSMYGGRQF
210 220 230 240 250
ATYDQGWVGW FCQKAVEIKN GINKPFTISG NGKQVRDVLH AEDMISLYFT
260 270 280 290 300
ALANVSKIRG NAFNIGGTIV NSLSLLELFK LLEDYCNIDM RFTNLPVRES
310 320 330
DQRVFVADIK KITNAIDWSP KVSAKDGVQK MYDWTSSI
Length:338
Mass (Da):37,958
Last modified:February 1, 1996 - v2
Checksum:i031175EDCF533888
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29682 Genomic DNA. Translation: AAB49384.1.
AL513382 Genomic DNA. Translation: CAD02451.1.
AE014613 Genomic DNA. Translation: AAO68475.1.
PIRiB33604.
RefSeqiNP_456637.1. NC_003198.1.
WP_000770936.1. NZ_LUHL01000038.1.

Genome annotation databases

EnsemblBacteriaiAAO68475; AAO68475; t0784.
CAD02451; CAD02451; CAD02451.
GeneIDi1248633.
KEGGistt:t0784.
sty:STY2298.
PATRICi18542537. VBISalEnt120419_2318.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29682 Genomic DNA. Translation: AAB49384.1.
AL513382 Genomic DNA. Translation: CAD02451.1.
AE014613 Genomic DNA. Translation: AAO68475.1.
PIRiB33604.
RefSeqiNP_456637.1. NC_003198.1.
WP_000770936.1. NZ_LUHL01000038.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ORRX-ray1.50A/B/C/D2-338[»]
ProteinModelPortaliP14169.
SMRiP14169. Positions 2-338.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi220341.STY2298.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAO68475; AAO68475; t0784.
CAD02451; CAD02451; CAD02451.
GeneIDi1248633.
KEGGistt:t0784.
sty:STY2298.
PATRICi18542537. VBISalEnt120419_2318.

Phylogenomic databases

eggNOGiENOG4108IAJ. Bacteria.
COG0451. LUCA.
HOGENOMiHOG000167994.
KOiK12454.
OMAiDFANTIR.
OrthoDBiEOG6TXQT1.

Enzyme and pathway databases

UniPathwayiUPA00055; UER00515.
BioCyciMetaCyc:MONOMER-13795.

Miscellaneous databases

EvolutionaryTraceiP14169.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF16363. GDP_Man_Dehyd. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae."
    Verma N., Reeves P.R.
    J. Bacteriol. 171:5694-5701(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.
  2. Reeves P.R.
    Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CT18.
  4. "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18."
    Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R.
    J. Bacteriol. 185:2330-2337(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700931 / Ty2.
  5. "High resolution X-ray structure of tyvelose epimerase from Salmonella typhi."
    Koropatkin N.M., Liu H.-W., Holden H.M.
    J. Biol. Chem. 278:20874-20881(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) OF 2-338 IN COMPLEX WITH NAD AND SUBSTRATE, SUBUNIT.

Entry informationi

Entry nameiRFBE_SALTI
AccessioniPrimary (citable) accession number: P14169
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.