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P14165

- CISY_PSEAE

UniProt

P14165 - CISY_PSEAE

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Protein

Citrate synthase

Gene

gltA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Enzyme regulationi

Allosterically inhibited by NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei306 – 3061PROSITE-ProRule annotation
Active sitei363 – 3631PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro
  2. citrate synthase activity Source: PseudoCAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:PA1580
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA1580.

Subcellular locationi

GO - Cellular componenti

  1. tricarboxylic acid cycle enzyme complex Source: PseudoCAP
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Citrate synthasePRO_0000169952Add
BLAST

Proteomic databases

PRIDEiP14165.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi208964.PA1580.

Structurei

3D structure databases

ProteinModelPortaliP14165.
SMRiP14165. Positions 3-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiP14165.
KOiK01647.
OMAiKETCDEV.
OrthoDBiEOG6P8TP4.
PhylomeDBiP14165.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14165-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADKKAQLII EGSAPVELPV LSGTMGPDVV DVRGLTATGH FTFDPGFMST
60 70 80 90 100
ASCESKITYI DGDKGVLLHR GYPIEQLAEK SDYLETCYLL LNGELPTAAQ
110 120 130 140 150
KEQFVGTIKN HTMVHEQLKT FFNGFRRDAH PMAVMCGVIG ALSAFYHDSL
160 170 180 190 200
DINNPKHREV SAHRLIAKMP TIAAMVYKYS KGEPMMYPRN DLNYAENFLH
210 220 230 240 250
MMFNTPCETK PISPVLAKAM DRIFILHADH EQNASTSTVR LAGSSGANPF
260 270 280 290 300
ACIASGIAAL WGPAHGGANE AVLRMLDEIG DVSNIDKFVE KAKDKNDPFK
310 320 330 340 350
LMGFGHRVYK NFDPRAKVMK QTCDEVLQEL GINDPQLELA MKLEEIARHD
360 370 380 390 400
PYFVERNLYP NVDFYSGIIL KAIGIPTSMF TVIFALARTV GWISHWQEML
410 420
SGPYKIGRPR QLYTGHTQRD FTALKDRG
Length:428
Mass (Da):47,695
Last modified:December 8, 2000 - v2
Checksum:i3E1875ED70266C37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531N → T in AAA25769. (PubMed:2507528)Curated
Sequence conflicti159 – 1591E → Q in AAA25769. (PubMed:2507528)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29728 Genomic DNA. Translation: AAA25769.1.
AE004091 Genomic DNA. Translation: AAG04969.1.
PIRiA33596. YKPSCA.
B83448.
RefSeqiNP_250271.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04969; AAG04969; PA1580.
GeneIDi882117.
KEGGipae:PA1580.
PATRICi19837506. VBIPseAer58763_1639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29728 Genomic DNA. Translation: AAA25769.1 .
AE004091 Genomic DNA. Translation: AAG04969.1 .
PIRi A33596. YKPSCA.
B83448.
RefSeqi NP_250271.1. NC_002516.2.

3D structure databases

ProteinModelPortali P14165.
SMRi P14165. Positions 3-427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA1580.

Proteomic databases

PRIDEi P14165.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG04969 ; AAG04969 ; PA1580 .
GeneIDi 882117.
KEGGi pae:PA1580.
PATRICi 19837506. VBIPseAer58763_1639.

Organism-specific databases

PseudoCAPi PA1580.

Phylogenomic databases

eggNOGi COG0372.
HOGENOMi HOG000021224.
InParanoidi P14165.
KOi K01647.
OMAi KETCDEV.
OrthoDBi EOG6P8TP4.
PhylomeDBi P14165.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00717 .

Family and domain databases

Gene3Di 1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProi IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view ]
PANTHERi PTHR11739. PTHR11739. 1 hit.
Pfami PF00285. Citrate_synt. 1 hit.
[Graphical view ]
PIRSFi PIRSF001369. Citrate_synth. 1 hit.
PRINTSi PR00143. CITRTSNTHASE.
SUPFAMi SSF48256. SSF48256. 1 hit.
TIGRFAMsi TIGR01798. cit_synth_I. 1 hit.
PROSITEi PS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa."
    Donald L.J., Molgat G.F., Duckworth H.W.
    J. Bacteriol. 171:5542-5550(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiCISY_PSEAE
AccessioniPrimary (citable) accession number: P14165
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 8, 2000
Last modified: October 29, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3