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P14165 (CISY_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Citrate synthase

EC=2.3.3.16
Gene names
Name:gltA
Ordered Locus Names:PA1580
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length428 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.

Enzyme regulation

Allosterically inhibited by NADH.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 1/2.

Subunit structure

Homohexamer.

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Sequence similarities

Belongs to the citrate synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 428428Citrate synthase
PRO_0000169952

Sites

Active site3061 By similarity
Active site3631 By similarity

Experimental info

Sequence conflict1531N → T in AAA25769. Ref.1
Sequence conflict1591E → Q in AAA25769. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P14165 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 3E1875ED70266C37

FASTA42847,695
        10         20         30         40         50         60 
MADKKAQLII EGSAPVELPV LSGTMGPDVV DVRGLTATGH FTFDPGFMST ASCESKITYI 

        70         80         90        100        110        120 
DGDKGVLLHR GYPIEQLAEK SDYLETCYLL LNGELPTAAQ KEQFVGTIKN HTMVHEQLKT 

       130        140        150        160        170        180 
FFNGFRRDAH PMAVMCGVIG ALSAFYHDSL DINNPKHREV SAHRLIAKMP TIAAMVYKYS 

       190        200        210        220        230        240 
KGEPMMYPRN DLNYAENFLH MMFNTPCETK PISPVLAKAM DRIFILHADH EQNASTSTVR 

       250        260        270        280        290        300 
LAGSSGANPF ACIASGIAAL WGPAHGGANE AVLRMLDEIG DVSNIDKFVE KAKDKNDPFK 

       310        320        330        340        350        360 
LMGFGHRVYK NFDPRAKVMK QTCDEVLQEL GINDPQLELA MKLEEIARHD PYFVERNLYP 

       370        380        390        400        410        420 
NVDFYSGIIL KAIGIPTSMF TVIFALARTV GWISHWQEML SGPYKIGRPR QLYTGHTQRD 


FTALKDRG 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa."
Donald L.J., Molgat G.F., Duckworth H.W.
J. Bacteriol. 171:5542-5550(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M29728 Genomic DNA. Translation: AAA25769.1.
AE004091 Genomic DNA. Translation: AAG04969.1.
PIRYKPSCA. A33596.
B83448.
RefSeqNP_250271.1. NC_002516.2.

3D structure databases

ProteinModelPortalP14165.
SMRP14165. Positions 3-427.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA1580.

Proteomic databases

PRIDEP14165.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG04969; AAG04969; PA1580.
GeneID882117.
KEGGpae:PA1580.
PATRIC19837506. VBIPseAer58763_1639.

Organism-specific databases

PseudoCAPPA1580.

Phylogenomic databases

eggNOGCOG0372.
HOGENOMHOG000021224.
KOK01647.
OMAKETCDEV.
OrthoDBEOG6P8TP4.
PhylomeDBP14165.

Enzyme and pathway databases

UniPathwayUPA00223; UER00717.

Family and domain databases

Gene3D1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-II.
[Graphical view]
PANTHERPTHR11739. PTHR11739. 1 hit.
PfamPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFPIRSF001369. Citrate_synth. 1 hit.
PRINTSPR00143. CITRTSNTHASE.
SUPFAMSSF48256. SSF48256. 1 hit.
TIGRFAMsTIGR01798. cit_synth_I. 1 hit.
PROSITEPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCISY_PSEAE
AccessionPrimary (citable) accession number: P14165
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 8, 2000
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways