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Protein

Citrate synthase

Gene

gltA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Enzyme regulationi

Allosterically inhibited by NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei306 – 3061PROSITE-ProRule annotation
Active sitei363 – 3631PROSITE-ProRule annotation

GO - Molecular functioni

  1. citrate (Si)-synthase activity Source: InterPro
  2. citrate synthase activity Source: PseudoCAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. tricarboxylic acid cycle Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:PA1580
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1580.

Subcellular locationi

GO - Cellular componenti

  1. tricarboxylic acid cycle enzyme complex Source: PseudoCAP
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 428428Citrate synthasePRO_0000169952Add
BLAST

Proteomic databases

PRIDEiP14165.

Interactioni

Subunit structurei

Homohexamer.

Protein-protein interaction databases

STRINGi208964.PA1580.

Structurei

3D structure databases

ProteinModelPortaliP14165.
SMRiP14165. Positions 3-427.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiP14165.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.
PhylomeDBiP14165.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P14165-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADKKAQLII EGSAPVELPV LSGTMGPDVV DVRGLTATGH FTFDPGFMST
60 70 80 90 100
ASCESKITYI DGDKGVLLHR GYPIEQLAEK SDYLETCYLL LNGELPTAAQ
110 120 130 140 150
KEQFVGTIKN HTMVHEQLKT FFNGFRRDAH PMAVMCGVIG ALSAFYHDSL
160 170 180 190 200
DINNPKHREV SAHRLIAKMP TIAAMVYKYS KGEPMMYPRN DLNYAENFLH
210 220 230 240 250
MMFNTPCETK PISPVLAKAM DRIFILHADH EQNASTSTVR LAGSSGANPF
260 270 280 290 300
ACIASGIAAL WGPAHGGANE AVLRMLDEIG DVSNIDKFVE KAKDKNDPFK
310 320 330 340 350
LMGFGHRVYK NFDPRAKVMK QTCDEVLQEL GINDPQLELA MKLEEIARHD
360 370 380 390 400
PYFVERNLYP NVDFYSGIIL KAIGIPTSMF TVIFALARTV GWISHWQEML
410 420
SGPYKIGRPR QLYTGHTQRD FTALKDRG
Length:428
Mass (Da):47,695
Last modified:December 8, 2000 - v2
Checksum:i3E1875ED70266C37
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti153 – 1531N → T in AAA25769 (PubMed:2507528).Curated
Sequence conflicti159 – 1591E → Q in AAA25769 (PubMed:2507528).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29728 Genomic DNA. Translation: AAA25769.1.
AE004091 Genomic DNA. Translation: AAG04969.1.
PIRiA33596. YKPSCA.
B83448.
RefSeqiNP_250271.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04969; AAG04969; PA1580.
GeneIDi882117.
KEGGipae:PA1580.
PATRICi19837506. VBIPseAer58763_1639.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29728 Genomic DNA. Translation: AAA25769.1.
AE004091 Genomic DNA. Translation: AAG04969.1.
PIRiA33596. YKPSCA.
B83448.
RefSeqiNP_250271.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP14165.
SMRiP14165. Positions 3-427.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1580.

Proteomic databases

PRIDEiP14165.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04969; AAG04969; PA1580.
GeneIDi882117.
KEGGipae:PA1580.
PATRICi19837506. VBIPseAer58763_1639.

Organism-specific databases

PseudoCAPiPA1580.

Phylogenomic databases

eggNOGiCOG0372.
HOGENOMiHOG000021224.
InParanoidiP14165.
KOiK01647.
OMAiNKEDGVR.
OrthoDBiEOG6P8TP4.
PhylomeDBiP14165.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase-like.
IPR016141. Citrate_synthase-like_core.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
IPR010953. Citrate_synthase_typ-I.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01798. cit_synth_I. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequencing, and expression of the gene for NADH-sensitive citrate synthase of Pseudomonas aeruginosa."
    Donald L.J., Molgat G.F., Duckworth H.W.
    J. Bacteriol. 171:5542-5550(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiCISY_PSEAE
AccessioniPrimary (citable) accession number: P14165
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 8, 2000
Last modified: January 7, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.