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Protein

ARS-binding factor 1

Gene

ABF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

General regulatory factor (GRF) that contributes to transcriptional activation of a large number of genes, as well as to DNA replication, silencing and telomere structure. Involved in the transcription activation of a subset of ribosomal protein genes. Binds the ARS-elements found in many promoters. Binds to the sequence 5'-TCN7ACG-3'. Influences on genome-wide nucleosome occupancy and affects chromatin structure, and probably dynamics. As a component of the global genome repair (GGR) complex, promotes global genome nucleotide excision repair (GG-NER) which removes DNA damage from nontranscribing DNA. Component of the regulatory network controlling mitotic and meiotic cell cycle progression.12 Publications

GO - Molecular functioni

  • core promoter sequence-specific DNA binding Source: SGD
  • DNA replication origin binding Source: SGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  • sequence-specific DNA binding Source: SGD
  • sequence-specific DNA binding, bending Source: SGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: SGD

GO - Biological processi

  • chromatin remodeling Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • DNA-dependent DNA replication Source: SGD
  • global genome nucleotide-excision repair Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

DNA damage, DNA repair, DNA replication, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31897-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ARS-binding factor 1
Alternative name(s):
Bidirectionally acting factor 1
DNA replication enhancer-binding protein OBF1
SFB-B
Gene namesi
Name:ABF1
Synonyms:BAF1, OBF1, REB2, SBF1
Ordered Locus Names:YKL112W
ORF Names:YKL505
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL112W.
SGDiS000001595. ABF1.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • nucleotide-excision repair factor 4 complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57H → Q: Loss of DNA binding. 1 Publication1
Mutagenesisi71C → S: Loss of DNA binding. 1 Publication1
Mutagenesisi625K → I: Leads to mislocalization into the cytoplasm. 1 Publication1
Mutagenesisi720S → A: Strongly reduces phosphorylation by CK2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000648071 – 731ARS-binding factor 1Add BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki18Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei189PhosphothreonineCombined sources1
Modified residuei193PhosphoserineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei554PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1
Modified residuei624Phosphoserine; by PKCSequence analysis1
Modified residuei720Phosphoserine; by CK2Combined sources1 Publication1

Post-translational modificationi

Extensively phosphorylated on Ser and Thr residues.2 Publications

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP14164.
PRIDEiP14164.

PTM databases

iPTMnetiP14164.

Interactioni

Subunit structurei

Component of the global genome repair (GGR) complex composed of at least ABF1, RAD7 and RAD16. Interacts with PSE1.2 Publications

Protein-protein interaction databases

BioGridi34022. 40 interactors.
DIPiDIP-2199N.
IntActiP14164. 26 interactors.
MINTiMINT-2783533.

Structurei

3D structure databases

ProteinModelPortaliP14164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni624 – 628C-terminal sequence 15
Regioni639 – 662C-terminal sequence 2Add BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi94 – 294Asn-richAdd BLAST201
Compositional biasi449 – 510Asn-richAdd BLAST62

Domaini

Contains 2 important clusters of amino acid residues in the C terminus (C-terminal sequence 1 or CS1, residues 624 to 628; and CS2, residues 639 to 662). CS1 specifically participates in transcriptional silencing and/or repression in a context-dependent manner, whereas CS2 is universally required for all functions of ABF1.1 Publication

Sequence similaritiesi

Belongs to the BAF1 family.Curated

Phylogenomic databases

InParanoidiP14164.
KOiK09249.
OMAiACHLKNC.
OrthoDBiEOG092C4E2Y.

Sequencei

Sequence statusi: Complete.

P14164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLVVNYYE YKHPIINKDL AIGAHGGKKF PTLGAWYDVI NEYEFQTRCP
60 70 80 90 100
IILKNSHRNK HFTFACHLKN CPFKVLLSYA GNAASSETSS PSANNNTNPP
110 120 130 140 150
GTPDHIHHHS NNMNNEDNDN NNGSNNKVSN DSKLDFVTDD LEYHLANTHP
160 170 180 190 200
DDTNDKVESR SNEVNGNNDD DADANNIFKQ QGVTIKNDTE DDSINKASID
210 220 230 240 250
RGLDDESGPT HGNDSGNHRH NEEDDVHTQM TKNYSDVVND EDINVAIANA
260 270 280 290 300
VANVDSQSNN KHDGKDDDAT NNNDGQDNNT NNDHNNNSNI NNNNVGSHGI
310 320 330 340 350
SSHSPSSIRD TSMNLDVFNS ATDDIPGPFV VTKIEPYHSH PLEDNLSLGK
360 370 380 390 400
FILTKIPKIL QNDLKFDQIL ESSYNNSNHT VSKFKVSHYV EESGLLDILM
410 420 430 440 450
QRYGLTAEDF EKRLLSQIAR RITTYKARFV LKKKKMGEYN DLQPSSSSNN
460 470 480 490 500
NNNNDGELSG TNLRSNSIDY AKHQEISSAG TSSNTTKNVN NNKNDSNDDN
510 520 530 540 550
NGNNNNDASN LMESVLDKTS SHRYQPKKMP SVNKWSKPDQ ITHSDVSMVG
560 570 580 590 600
LDESNDGGNE NVHPTLAEVD AQEARETAQL AIDKINSYKR SIDDKNGDGH
610 620 630 640 650
NNSSRNVVDE NLINDMDSED AHKSKRQHLS DITLEERNED DKLPHEVAEQ
660 670 680 690 700
LRLLSSHLKE VENLHQNNDD DVDDVMVDVD VESQYNKNTT HHNNHHSQPH
710 720 730
HDEEDVAGLI GKADDEEDLS DENIQPELRG Q
Length:731
Mass (Da):81,753
Last modified:October 5, 2010 - v4
Checksum:iBE338DA7C9514DC3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti125N → K in CAA34421 (PubMed:2686983).Curated1
Sequence conflicti128V → A in CAA35966 (PubMed:2620828).Curated1
Sequence conflicti128V → A in AAA66311 (PubMed:2511628).Curated1
Sequence conflicti128V → A in AAA34823 (PubMed:2034654).Curated1
Sequence conflicti148T → I in CAA35966 (PubMed:2620828).Curated1
Sequence conflicti148T → I in AAA66311 (PubMed:2511628).Curated1
Sequence conflicti148T → I in AAA34823 (PubMed:2034654).Curated1
Sequence conflicti279 – 280NT → TN in CAA35966 (PubMed:2620828).Curated2
Sequence conflicti279 – 280NT → TN in AAA66311 (PubMed:2511628).Curated2
Sequence conflicti279 – 280NT → TN in AAA34823 (PubMed:2034654).Curated2
Sequence conflicti690T → N in CAA35966 (PubMed:2620828).Curated1
Sequence conflicti690T → N in AAA66311 (PubMed:2511628).Curated1
Sequence conflicti690T → N in AAA34823 (PubMed:2034654).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16385 Genomic DNA. Translation: CAA34421.1.
X51654 Genomic DNA. Translation: CAA35966.1.
M29067 Genomic DNA. Translation: AAA66311.1.
M63578 Genomic DNA. Translation: AAA34823.1.
S93804 Genomic DNA. Translation: AAB22002.1.
Z28111 Genomic DNA. Translation: CAA81951.1.
X77511 Genomic DNA. Translation: CAA54647.1.
BK006944 Genomic DNA. Translation: DAA09046.1.
PIRiS29870.
RefSeqiNP_012810.1. NM_001179678.1.

Genome annotation databases

EnsemblFungiiYKL112W; YKL112W; YKL112W.
GeneIDi853748.
KEGGisce:YKL112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X16385 Genomic DNA. Translation: CAA34421.1.
X51654 Genomic DNA. Translation: CAA35966.1.
M29067 Genomic DNA. Translation: AAA66311.1.
M63578 Genomic DNA. Translation: AAA34823.1.
S93804 Genomic DNA. Translation: AAB22002.1.
Z28111 Genomic DNA. Translation: CAA81951.1.
X77511 Genomic DNA. Translation: CAA54647.1.
BK006944 Genomic DNA. Translation: DAA09046.1.
PIRiS29870.
RefSeqiNP_012810.1. NM_001179678.1.

3D structure databases

ProteinModelPortaliP14164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34022. 40 interactors.
DIPiDIP-2199N.
IntActiP14164. 26 interactors.
MINTiMINT-2783533.

PTM databases

iPTMnetiP14164.

Proteomic databases

MaxQBiP14164.
PRIDEiP14164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL112W; YKL112W; YKL112W.
GeneIDi853748.
KEGGisce:YKL112W.

Organism-specific databases

EuPathDBiFungiDB:YKL112W.
SGDiS000001595. ABF1.

Phylogenomic databases

InParanoidiP14164.
KOiK09249.
OMAiACHLKNC.
OrthoDBiEOG092C4E2Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-31897-MONOMER.

Miscellaneous databases

PROiP14164.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiABF1_YEAST
AccessioniPrimary (citable) accession number: P14164
Secondary accession number(s): D6VXH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: October 5, 2010
Last modified: November 2, 2016
This is version 148 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4820 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.