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Protein

Gap junction alpha-1 protein

Gene

GJA1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Plays an essential role in gap junction communication in the ventricles.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Gap junction alpha-1 protein
Alternative name(s):
Connexin-43
Short name:
Cx43
Gene namesi
Name:GJA1
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein Sequence analysis
  • Cell junctiongap junction By similarity

  • Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and proper localization at ICD is dependent on TMEM65.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1312CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3623HelicalSequence analysisAdd
BLAST
Topological domaini37 – 7640ExtracellularSequence analysisAdd
BLAST
Transmembranei77 – 9923HelicalSequence analysisAdd
BLAST
Topological domaini100 – 15455CytoplasmicSequence analysisAdd
BLAST
Transmembranei155 – 17723HelicalSequence analysisAdd
BLAST
Topological domaini178 – 20831ExtracellularSequence analysisAdd
BLAST
Transmembranei209 – 23123HelicalSequence analysisAdd
BLAST
Topological domaini232 – 381150CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Gap junction, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 381380Gap junction alpha-1 proteinPRO_0000057805Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 192By similarity
Disulfide bondi187 ↔ 198By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP14154.

Expressioni

Tissue specificityi

In all tissues, but mostly in lens.

Interactioni

Subunit structurei

A connexon is composed of a hexamer of connexins. Interacts with TMEM65.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000023950.

Structurei

3D structure databases

ProteinModelPortaliP14154.
SMRiP14154. Positions 254-381.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IF97. Eukaryota.
ENOG4110JTW. LUCA.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP14154.
KOiK07372.
PhylomeDBiP14154.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002261. Connexin43.
IPR013124. Connexin43_C.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF03508. Connexin43. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01132. CONNEXINA1.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14154-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS
60 70 80 90 100
AFRCNTQQPG CENVCYDKSF PISHVRFWVL QIIFVSVPTL LYLAHVFYVM
110 120 130 140 150
RKEEKLNKRE EELKVVQNDG VNVDMHLKQI ESKKFKYGIE EHGKVKMRGG
160 170 180 190 200
LLRTYIISIL FKSVFEVAFL LIQWYIYGFS LSAIYTCERD PCPHRVDCFL
210 220 230 240 250
SRPTEKTIFI VFMLVVSLVS LALNIIELFY VFFKGVKDRV KGKTDPYSHS
260 270 280 290 300
GTMSPSKDCG SPKYAYYNGC SSPTAPLSPM SPPGYKLVTG DRNNSSCRNY
310 320 330 340 350
NKQASEQNWA NYSAEQNRMG QAGSTISNSH AQPFDFADEH QNTKKLASGH
360 370 380
ELQPLTIVDQ RPPSRASSRA SSRPRPDDLE I
Length:381
Mass (Da):43,125
Last modified:January 23, 2007 - v3
Checksum:i6714ED97A17DB75F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321S → I in AAK58512 (PubMed:12487409).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29003 mRNA. Translation: AAA48715.2.
AF233738 Genomic DNA. Translation: AAK58512.1.
PIRiA48171.
RefSeqiNP_989917.1. NM_204586.2.
UniGeneiGga.3195.

Genome annotation databases

GeneIDi395278.
KEGGigga:395278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29003 mRNA. Translation: AAA48715.2.
AF233738 Genomic DNA. Translation: AAK58512.1.
PIRiA48171.
RefSeqiNP_989917.1. NM_204586.2.
UniGeneiGga.3195.

3D structure databases

ProteinModelPortaliP14154.
SMRiP14154. Positions 254-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000023950.

Proteomic databases

PaxDbiP14154.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395278.
KEGGigga:395278.

Organism-specific databases

CTDi2697.

Phylogenomic databases

eggNOGiENOG410IF97. Eukaryota.
ENOG4110JTW. LUCA.
HOGENOMiHOG000231127.
HOVERGENiHBG009576.
InParanoidiP14154.
KOiK07372.
PhylomeDBiP14154.

Miscellaneous databases

NextBioi20815366.
PROiP14154.

Family and domain databases

InterProiIPR000500. Connexin.
IPR002261. Connexin43.
IPR013124. Connexin43_C.
IPR019570. Connexin_CCC.
IPR017990. Connexin_CS.
IPR013092. Connexin_N.
[Graphical view]
PANTHERiPTHR11984. PTHR11984. 1 hit.
PfamiPF00029. Connexin. 1 hit.
PF03508. Connexin43. 1 hit.
[Graphical view]
PRINTSiPR00206. CONNEXIN.
PR01132. CONNEXINA1.
SMARTiSM00037. CNX. 1 hit.
SM01089. Connexin_CCC. 1 hit.
[Graphical view]
PROSITEiPS00407. CONNEXINS_1. 1 hit.
PS00408. CONNEXINS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Expression of the gap junction protein connexin43 in embryonic chick lens: molecular cloning, ultrastructural localization, and post-translational phosphorylation."
    Musil L.S., Beyer E.C., Goodenough D.A.
    J. Membr. Biol. 116:163-175(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Lens.
  2. Beyer E.C., Berthoud V.M.
    Submitted (DEC-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 49-50 AND 132.
  3. "The highly conserved Gln49 and Ser50 of mammalian connexin43 are present in chick connexin43 and essential for functional gap junction channels."
    Sokolova I.V., Martinez A.-M., Fletcher W.H.
    Cell Commun. Adhes. 9:75-86(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 13-146.

Entry informationi

Entry nameiCXA1_CHICK
AccessioniPrimary (citable) accession number: P14154
Secondary accession number(s): Q90ZE0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: January 20, 2016
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.