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Protein

Malate dehydrogenase, cytoplasmic

Gene

Mdh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei105NADBy similarity1
Binding sitei112NADBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei162SubstrateBy similarity1
Active sitei187Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADBy similarity7
Nucleotide bindingi129 – 131NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:Mdh1
Synonyms:Mor2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:97051 Mdh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176827

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001134102 – 334Malate dehydrogenase, cytoplasmicAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei110N6-succinyllysineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei121N6-acetyllysineBy similarity1
Modified residuei214N6-succinyllysineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei230Omega-N-methylarginineCombined sources1
Modified residuei241PhosphoserineBy similarity1
Modified residuei298N6-acetyllysine; alternateBy similarity1
Modified residuei298N6-succinyllysine; alternateCombined sources1
Modified residuei309PhosphoserineCombined sources1
Modified residuei318N6-succinyllysineCombined sources1
Modified residuei332PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1

Post-translational modificationi

ISGylated.1 Publication
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14152
MaxQBiP14152
PaxDbiP14152
PeptideAtlasiP14152
PRIDEiP14152
TopDownProteomicsiP14152

2D gel databases

COMPLUYEAST-2DPAGEiP14152
REPRODUCTION-2DPAGEiP14152
SWISS-2DPAGEiP14152
UCD-2DPAGEiP14152

PTM databases

iPTMnetiP14152
PhosphoSitePlusiP14152
SwissPalmiP14152

Expressioni

Gene expression databases

BgeeiENSMUSG00000020321
CleanExiMM_MDH1
ExpressionAtlasiP14152 baseline and differential
GenevisibleiP14152 MM

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi201468, 1 interactor
IntActiP14152, 9 interactors
MINTiP14152
STRINGi10090.ENSMUSP00000099938

Structurei

3D structure databases

ProteinModelPortaliP14152
SMRiP14152
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496 Eukaryota
COG0039 LUCA
GeneTreeiENSGT00530000063410
HOGENOMiHOG000220953
HOVERGENiHBG006340
InParanoidiP14152
KOiK00025
OMAiRPRTKGM
OrthoDBiEOG091G0BLA
PhylomeDBiP14152
TreeFamiTF105826

Family and domain databases

CDDicd01336 MDH_cytoplasmic_cytosolic, 1 hit
Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR011274 Malate_DH_NAD-dep_euk
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
TIGR01758 MDH_euk_cyt, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLQDVIA TDKEEIAFKD LDVAVLVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGTALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
ADHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA
Length:334
Mass (Da):36,511
Last modified:January 23, 2007 - v3
Checksum:i3B9F00372AA939DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92R → I in BAB23897 (PubMed:16141072).Curated1
Sequence conflicti177D → N in BAE37915 (PubMed:16141072).Curated1
Sequence conflicti288F → L (PubMed:3312200).Curated1
Sequence conflicti288F → L (PubMed:3172222).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29462 mRNA Translation: AAA39510.1
M36084 Genomic DNA Translation: AAA37423.1
AK005237 mRNA Translation: BAB23897.1
AK164785 mRNA Translation: BAE37915.1
AK168545 mRNA Translation: BAE40421.1
AL663049 Genomic DNA Translation: CAI24411.1
BC050940 mRNA Translation: AAH50940.2
CCDSiCCDS24466.1
PIRiS02654 DEMSMC
RefSeqiNP_001303604.1, NM_001316675.1
NP_032644.3, NM_008618.3
UniGeneiMm.212703

Genome annotation databases

EnsembliENSMUST00000102874; ENSMUSP00000099938; ENSMUSG00000020321
GeneIDi17449
KEGGimmu:17449
UCSCiuc007idv.2 mouse

Similar proteinsi

Entry informationi

Entry nameiMDHC_MOUSE
AccessioniPrimary (citable) accession number: P14152
Secondary accession number(s): Q3TP22, Q80Y13, Q9DB45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 170 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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