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Protein

Malate dehydrogenase, cytoplasmic

Gene

Mdh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei105NADBy similarity1
Binding sitei112NADBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei162SubstrateBy similarity1
Active sitei187Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 17NADBy similarity7
Nucleotide bindingi129 – 131NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, cytoplasmic (EC:1.1.1.37)
Alternative name(s):
Cytosolic malate dehydrogenase
Gene namesi
Name:Mdh1
Synonyms:Mor2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:97051. Mdh1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: MGI
  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
  • mitochondrion Source: MGI
  • myelin sheath Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176827.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001134102 – 334Malate dehydrogenase, cytoplasmicAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei110N6-succinyllysineCombined sources1
Modified residuei118N6-acetyllysineCombined sources1
Modified residuei121N6-acetyllysineBy similarity1
Modified residuei214N6-succinyllysineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei230Omega-N-methylarginineCombined sources1
Modified residuei241PhosphoserineBy similarity1
Modified residuei298N6-acetyllysine; alternateBy similarity1
Modified residuei298N6-succinyllysine; alternateCombined sources1
Modified residuei309PhosphoserineCombined sources1
Modified residuei318N6-succinyllysineCombined sources1
Modified residuei332PhosphoserineBy similarity1
Modified residuei333PhosphoserineBy similarity1

Post-translational modificationi

ISGylated.1 Publication
Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP14152.
MaxQBiP14152.
PaxDbiP14152.
PeptideAtlasiP14152.
PRIDEiP14152.
TopDownProteomicsiP14152.

2D gel databases

COMPLUYEAST-2DPAGEP14152.
REPRODUCTION-2DPAGEP14152.
SWISS-2DPAGEP14152.
UCD-2DPAGEP14152.

PTM databases

iPTMnetiP14152.
PhosphoSitePlusiP14152.
SwissPalmiP14152.

Expressioni

Gene expression databases

BgeeiENSMUSG00000020321.
CleanExiMM_MDH1.
ExpressionAtlasiP14152. baseline and differential.
GenevisibleiP14152. MM.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi201468. 1 interactor.
IntActiP14152. 8 interactors.
MINTiMINT-1869753.
STRINGi10090.ENSMUSP00000099938.

Structurei

3D structure databases

ProteinModelPortaliP14152.
SMRiP14152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.Curated

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiP14152.
KOiK00025.
OMAiQYPDAGQ.
OrthoDBiEOG091G0BLA.
PhylomeDBiP14152.
TreeFamiTF105826.

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P14152-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL
60 70 80 90 100
DGVLMELQDC ALPLLQDVIA TDKEEIAFKD LDVAVLVGSM PRREGMERKD
110 120 130 140 150
LLKANVKIFK SQGTALEKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE
160 170 180 190 200
NFSCLTRLDH NRAKSQIALK LGVTADDVKN VIIWGNHSST QYPDVNHAKV
210 220 230 240 250
KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL SSAMSAAKAI
260 270 280 290 300
ADHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV
310 320 330
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA
Length:334
Mass (Da):36,511
Last modified:January 23, 2007 - v3
Checksum:i3B9F00372AA939DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti92R → I in BAB23897 (PubMed:16141072).Curated1
Sequence conflicti177D → N in BAE37915 (PubMed:16141072).Curated1
Sequence conflicti288F → L (PubMed:3312200).Curated1
Sequence conflicti288F → L (PubMed:3172222).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29462 mRNA. Translation: AAA39510.1.
M36084 Genomic DNA. Translation: AAA37423.1.
AK005237 mRNA. Translation: BAB23897.1.
AK164785 mRNA. Translation: BAE37915.1.
AK168545 mRNA. Translation: BAE40421.1.
AL663049 Genomic DNA. Translation: CAI24411.1.
BC050940 mRNA. Translation: AAH50940.2.
CCDSiCCDS24466.1.
PIRiS02654. DEMSMC.
RefSeqiNP_001303604.1. NM_001316675.1.
NP_032644.3. NM_008618.3.
UniGeneiMm.212703.

Genome annotation databases

EnsembliENSMUST00000102874; ENSMUSP00000099938; ENSMUSG00000020321.
GeneIDi17449.
KEGGimmu:17449.
UCSCiuc007idv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M29462 mRNA. Translation: AAA39510.1.
M36084 Genomic DNA. Translation: AAA37423.1.
AK005237 mRNA. Translation: BAB23897.1.
AK164785 mRNA. Translation: BAE37915.1.
AK168545 mRNA. Translation: BAE40421.1.
AL663049 Genomic DNA. Translation: CAI24411.1.
BC050940 mRNA. Translation: AAH50940.2.
CCDSiCCDS24466.1.
PIRiS02654. DEMSMC.
RefSeqiNP_001303604.1. NM_001316675.1.
NP_032644.3. NM_008618.3.
UniGeneiMm.212703.

3D structure databases

ProteinModelPortaliP14152.
SMRiP14152.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201468. 1 interactor.
IntActiP14152. 8 interactors.
MINTiMINT-1869753.
STRINGi10090.ENSMUSP00000099938.

Chemistry databases

ChEMBLiCHEMBL2176827.

PTM databases

iPTMnetiP14152.
PhosphoSitePlusiP14152.
SwissPalmiP14152.

2D gel databases

COMPLUYEAST-2DPAGEP14152.
REPRODUCTION-2DPAGEP14152.
SWISS-2DPAGEP14152.
UCD-2DPAGEP14152.

Proteomic databases

EPDiP14152.
MaxQBiP14152.
PaxDbiP14152.
PeptideAtlasiP14152.
PRIDEiP14152.
TopDownProteomicsiP14152.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000102874; ENSMUSP00000099938; ENSMUSG00000020321.
GeneIDi17449.
KEGGimmu:17449.
UCSCiuc007idv.2. mouse.

Organism-specific databases

CTDi4190.
MGIiMGI:97051. Mdh1.

Phylogenomic databases

eggNOGiKOG1496. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00530000063410.
HOGENOMiHOG000220953.
HOVERGENiHBG006340.
InParanoidiP14152.
KOiK00025.
OMAiQYPDAGQ.
OrthoDBiEOG091G0BLA.
PhylomeDBiP14152.
TreeFamiTF105826.

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.

Miscellaneous databases

PROiP14152.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020321.
CleanExiMM_MDH1.
ExpressionAtlasiP14152. baseline and differential.
GenevisibleiP14152. MM.

Family and domain databases

CDDicd01336. MDH_cytoplasmic_cytosolic. 1 hit.
Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR011274. Malate_DH_NAD-dep_euk.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
TIGR01758. MDH_euk_cyt. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDHC_MOUSE
AccessioniPrimary (citable) accession number: P14152
Secondary accession number(s): Q3TP22, Q80Y13, Q9DB45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 163 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.