Reviewed, UniProtKB/Swiss-Prot P14149 (RR12_CHLRE)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S12, chloroplastic

Gene names

Name:

rps12

Encoded on

Plastid; Chloroplast

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	133 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits By similarity. HAMAP MF_00403
Subunit structure	Part of the 30S ribosomal subunit. HAMAP MF_00403
Subcellular location	Plastid › chloroplast HAMAP MF_00403.
Sequence similarities	Belongs to the ribosomal protein S12P family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Chloroplast Plastid
Ligand	RNA-binding rRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW translation Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell small ribosomal subunit Inferred from electronic annotation. Source: InterPro
Molecular function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 133	132	30S ribosomal protein S12, chloroplastic HAMAP MF_00403		PRO_0000146396
Experimental info
	Mutagenesis	14	1	K → T: Streptomycin resistance.
	Mutagenesis	91	1	P → L: Streptomycin dependence.

Sequences

	Sequence		Length	Mass (Da)
	P14149-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 22A4A5B782EAD62B	FASTA	133	14,603
	10 20 30 40 50 60 MPTIQQLIRS ARKKITKKTK SPALKSCPQR RGICLRVYTV TPKKPNSALR KVARVRLTTG 70 80 90 100 110 120 FEVTAYIPGV GHNLQEHAVV LVRGGRVKDL PGVRYHIVRG SLDTAGVKNR VQSRSKYGVK 130 MGSKTAAKTA GKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chloroplast ribosomal protein gene rps12 of Chlamydomonas reinhardtii. Wild-type sequence, mutation to streptomycin resistance and dependence, and function in Escherichia coli." Liu X.-Q., Gillham N.W., Boynton J.E. J. Biol. Chem. 264:16100-16108(1989) [PubMed: 2777778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], STREPTOMYCIN RESISTANT MUTANTS.
[2]	Hauser C.R., Boynton J.E., Gillham N.W. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide diversity in the chloroplast genome of Chlamydomonas reinhardtii." United States Department of Energy Joint Genome Institute Smith D.R. Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC-503.
[4]	"Proteomic characterization of the small subunit of Chlamydomonas reinhardtii chloroplast ribosome: identification of a novel S1 domain-containing protein and unusually large orthologs of bacterial S2, S3, and S5." Yamaguchi K., Prieto S., Beligni M.V., Haynes P.A., McDonald W.H., Yates J.R. III, Mayfield S.P. Plant Cell 14:2957-2974(2002) [PubMed: 12417713] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-9. Strain: Arg7/cw15.
[5]	"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B. Plant Cell 14:2659-2679(2002) [PubMed: 12417694] [Abstract] Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M29284 Genomic DNA. Translation: AAA84155.1. AF061851 Genomic DNA. Translation: AAC16329.1. FJ423446 Genomic DNA. Translation: ACJ50147.1. BK000554 Genomic DNA. Translation: DAA00960.1.
PIR	A34248.
RefSeq	NP_958416.1.
3D structure databases
SMR	P14149. Positions 1-120.
ModBase	Search...
Proteomic databases
PRIDE	P14149.
Genome annotation databases
GeneID	2717036.
KEGG	cre:ChreCp060.
Family and domain databases
HAMAP	MF_00403_B. Ribosomal_S12_B. [Tree]
InterPro	IPR016027. NA-bd_OB-fold-like. IPR006032. Ribosomal_S12/S23. IPR005679. Ribosomal_S12_bac-type. [Graphical view]
PANTHER	PTHR11652:SF1. Ribosom_S12_bac. 1 hit. PTHR11652. Ribosomal_S12_23. 1 hit.
Pfam	PF00164. Ribosomal_S12. 1 hit. [Graphical view]
PIRSF	PIRSF002133. Ribosomal_S12/S23. 1 hit.
PRINTS	PR01034. RIBOSOMALS12.
TIGRFAMs	TIGR00981. rpsL_bact. 1 hit.
PROSITE	PS00055. RIBOSOMAL_S12. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RR12_CHLRE

Accession

Primary (citable) accession number: P14149
Secondary accession number(s): B7U1K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)